Ɲɛnamaya taabolo bɛɛ kɔnɔ ani bi furakɛlicogo sintetiki, pepitiri jɛɲɔgɔnya de bɛ yen. O jɛɲɔgɔnya kovalɛn jusigilen in bɛ asidi aminikiw siri ɲɔgɔn na walasa ka fɛnɲɛnamaw ka mɔlikulu gɛlɛnw dilan. Ka taa kalan gafe ɲɛfɔli tɛmɛnenw na, ka nin kemikoro jɛɲɔgɔnya in dɔn, o bɛ jago ɲɛtaa jira biyoteknoloji la k’a ɲɛsin a yɛrɛ ma. Protéines naturelles synthèse bɛ baara kɛ cogo dafalen na selilu ribosome kɔnɔ, ka baarakɛcogo kabakoma sɔrɔ. O kɔfɛ, ka o jɛɲɔgɔnyaw ladege cogo dilannen na sɛgɛsɛgɛli la, o bɛ gɛlɛyabaw lase ɛntɛrinɛti ma. Sɛnɛfɛnw sɔrɔli dɔgɔyali, saniyako gɛlɛyaw, ani sabatili dɛsɛ bɛ to ka laboratuwari sigicogo ni jagokɛlaw ka fɛn dilanni siraw bɛɛ tɔɔrɔ. Aw ka kan ka jɛɲɔgɔnyaw jɔli ni u tigɛcogo kemikɛli tiɲɛw faamuya walasa ka sintezi fɛɛrɛw jateminɛ ka ɲɛ. An bɛna a sɛgɛsɛgɛ cogo min na sigidako gɛlɛya bɛ nɔ bila jateminɛw tiɲɛni ni u dilanni fɛɛrɛw la. A laban na, o hakilina nafamaw bɛna i bilasira ka fɛn dilanni jɛɲɔgɔnw sugandi minnu bɛ se kosɛbɛ ka kɛɲɛ ni ladamu ye pepitiriw (pepitiriw) ye.
Tablo kɔnɔkow
Structural Rigidity : Peptide jɛɲɔgɔnyaw bɛ jɛɲɔgɔnya fila cogoya yɔrɔ dɔ jira, o bɛ furakɛli ni fɛnɲɛnamafagalanw sabatili ni u cogoya 3D jira.
Sintezi Bugunw : Kondensasiyɔn kɛcogo minnu bɛ kɛ laboratuwari sigidaw la (i n’a fɔ SPPS) olu bɛ ɲɛnabɔli gɛlɛnw de wajibiya walasa ka ɲɔgɔndan tigɛlenw ni ɲɔgɔn dalajɛ bali.
Sɛgɛsɛgɛli wajibiyalenw : Ka jɛɲɔgɔnya dafalen sabati, o bɛ sɛgɛsɛgɛli barikamaw de wajibiya minnu bɛ kɛ ni chromatographie ni spectrométrie ye (HPLC, MS) walasa ka ɲininiw walima saniyacogo dɔn min bɛ se ka kɛ kɛnɛyaso la.
Jɛɲɔgɔn sugandili : Ka bɔ hakilina na ka taa jago la, o bɛ jɛɲɔgɔn sinsinni de wajibiya min jateminɛna sɛgɛsɛgɛli kɛcogo kan, QA/QC jɛlenya, ani faratiw dɔgɔyali fɛɛrɛw.
Biologique systemes bɛ farikolojɔlifɛn gɛlɛnw jɔ farikolojidɛsɛ kɛcogo tigitigi fɛ. Asidi aminiki kelen ka karɔkisili kulu bɛ baara Kɛ k’a ɲɛsin o kɛcogo ma. A bɛ siri asidi aminiki dɔ kɛrɛfɛ min bɛ amino kulu la. O kemikoro kɛrɛnkɛrɛnnen in bɛ ji molekilɛri kelen bɔ i n’a fɔ a kɔfɛfɛn. O jɛɲɔgɔnya kovalɛn min bɛ sɔrɔ o la, o bɛ kɛ bɛɛ kɔkolo fɔlɔ ye pepitiriw . A bɛ kɛ i n’a fɔ jɔli sɛgɛsɛgɛli jɔnjɔn min bɛ ɲɛnamaya minɛ ɲɔgɔn na. An bɛ o jɛɲɔgɔnya kɛrɛnkɛrɛnnen in Weele ko amidi jɛɲɔgɔnya. Kimikɛlaw y’a ka jogo kɛrɛnkɛrɛnnenw sɛgɛsɛgɛ kabini san tan caman walasa ka furaw dilanni faamuya.
Karɔbɔni ni azote jɛɲɔgɔnya tɛ jɛɲɔgɔnya kelen nɔgɔman ye. Elektɔronw bɛ delocalize (yɔrɔ wɛrɛ) kɛ tuma bɛɛ oksizɛni, karɔbɔni ani azote atomuw cɛma. O ɛlektrɔni-falen-falen in bɛ rezɔn-yɔrɔ danfaralen dɔ Dabɔ. O jɛɲɔgɔnya bɛ jɛɲɔgɔnya fila-fila cogoya dɔ sɔrɔ. A tɛ Se ka Yɛlɛma-yɛlɛma hɔrɔnya la sankolo la. Atomu wɔɔrɔ minnu bɛ o ko la, olu bɛ To u datugulen na kudayi jateminɛ gɛlɛn na, min bɛ Kɛ ni 'plan ye. Trans configuration (Trans configuration) ka di kosɛbɛ a yɛrɛ la. O bɛ steriki kɛlɛ juguw bali kɛrɛfɛ cakɛda belebelebaw ni ɲɔgɔn cɛ. Proline bɛ danfara jira min man teli ka kɛ yɔrɔ min na cis configurations bɛ kɛ. O jateminɛ-dakun in bɛ dan sigi kosɛbɛ molekiyɔmuw ka sɛgɛsɛgɛli la.
Mun na o gɛlɛya danbe in nafa ka bon kosɛbɛ? A bɛ nɔ bila furakɛli sintetikiw dilanni n’u ɲɛtaa la k’a ɲɛsin u yɛrɛ ma. Yɛlɛmabaliya bɛ dantigɛli saba kɛrɛnkɛrɛnnenw wajibiya. O cogoya minnu bɛ se ka fɔ ka ɲɛ, olu bɛ selilu minɛbagaw sirili ɲɔgɔndɛmɛ dantigɛ. Gɛlɛya fana bɛ molekiyɔmu kɔkolo tanga anzimu tiɲɛni teliya ma. A bɛ jagokɛla ka furaw dilanni waati janya. Ni ɲininikɛlaw bɛ fɛn dilannenw dilan, u bɛ u jigi da o jateminɛcogo kan min bɛ se ka fɔ ka ɲɛ. Planar nature bɛ biyoavailabilité in vivo bɛɛ lajɛlen di. Ni o sirili tun bɛ se ka wuli ka bɔ a nɔ na, fura minnu laɲininen don, olu tun tɛna se ka datugu selilɛriw kɔnɔ ka ɲɛ.
Biochimique (Biyokimiki) Fɛn min bɛ yen |
Fɛnɲɛnɛmafagalanw |
Jagokɛlaw ka kɔlɔlɔ |
|---|---|---|
Resonance Delocalisation (Jɛkulu min bɛ wele ko Resonance). |
Yɔrɔ fila-fila-yɔrɔ-ko |
A bɛ kɔkolo jiginni bali, ka jɔli sabatili wajibiya. |
Geometrie Planaire (Jɛkulu Planaire). |
Trans configuration (Trans configuration) labɛnna |
A bɛ dɔ bɔ kɛrɛfɛ-cakɛda ɲɔgɔndan na, ka jaabiw minɛbagaw ka ɲɔgɔndɛmɛ ɲɛ. |
Farikolojidɛsɛ Sintezi (Synthèse de hydratation). |
Kovalɛn kɔkolo jɛɲɔgɔnya |
A bɛ fanga doncogo kɛrɛnkɛrɛnnen de wajibiya, o bɛ sintetiki sɛgɛsɛgɛli gɛlɛya. |
Biologique translation bɛ baara kɛ ni selilɛri ribosome ye cogo dafalen na. Nin biologiki masin in bɛ baara kɛ kosɛbɛ cogo kabakoma na, wa a bɛ labɛn kosɛbɛ. A bɛ asidi aminikiw siri ɲɔgɔn na ji la sigidaw la k’a sɔrɔ a ma cɛsiri kɛ. Kemisɛnniw dilanni bɛ baara kɛ cogo wɛrɛ la kosɛbɛ laboratuwari kɔnɔ. Solid-Phase Peptide Synthesis (SPPS) bɛ kɛ bi jagokɛcogo ye. A bɛ fɛnɲɛnɛmafagalanw de wajibiya minnu ka gɛlɛn i n’a fɔ HATU walima DIC. An ka kan ka baarakɛjɛkulu kɛrɛnkɛrɛnnenw lakana a daminɛ na. Lakanali kulu minnu bɛ sɔrɔ tuma caman na, olu ye Fmoc walima Boc kemisɛriw ye. O kɔfɛ, an bɛ u lakanani bɔ a la ni basi walima asidi nɔgɔmanw ye. O sɛgɛsɛgɛli jugu in bɛ segin a kan asidi aminiki kelen-kelen bɛɛ la min farala a kan. Kemikɛli tiɲɛniw bɛ sigida lamini kɔlɔsili gɛlɛnw yamaruya minnu tɛ ji sɔrɔ. An ka kan ka force a reaction biological systems perform naturally.
Fɛnɲɛnɛmaw bɛ yen hali ni kovalɛn cogoya barika ka bon u danma. Cleavage, min ka teli ka kɛ ni hydrolyse ye, o bɛ to ka kɛ bagabagali ye tuma bɛɛ iziniw kɔnɔ. Lamini cogoya dɔw bɛ o kemikaliya tiɲɛni teliya joona. Aw ka kan ka nin tiɲɛni sira ninnu makɔnɔ furakɛli kɛtuma na.
pH caman bɛ sigida minnu na, olu bɛ balansi kɔrɔlenw tiɲɛ, o bɛ kɛ sababu ye ka jikuru teliya.
Protéolytique enzymes bɛ amino acids sequence motifs kɛrɛnkɛrɛnnenw laɲini kosɛbɛ ani ka u tigɛ.
Sumaya degunba bɛ girinya tɛmɛnen don a la, ka kɔkolo jɛɲɔgɔnyaw tiɲɛ ka kɔn a waati ɲɛ.
Mikorobiyɔmu nɔgɔli bɛ anzimu baara min ma labɛn, o don furakɛlicogo steriliw kɔnɔ.
Lamini ji tɛmɛnen bɛ jikuruw toli dɔɔni dɔɔni bila muguw la minnu datugulen don ka ɲɛ.
O sabatili danw jateminɛni bɛ sariyaw de wajibiya minnu bɛ se ka kɛ ni kuntilenna ye kosɛbɛ. Aw tɛ se ka jagofɛnw dilan fiyentɔya la. Cleavage faratiw bɛ maracogo gɛlɛnw yamaruya diɲɛ kɔnɔ. Misali la, mugu minnu bɛ kɛ ni liyofili ye, olu bɛ funteni hakɛ basigilen de wajibiya min bɛ zeru duguma. Jilafɛnw bɛ tampon kɛrɛnkɛrɛnnenw de wajibiya. Forokofɛnw ka kan ka ji ni UV yeelen bali kosɛbɛ. O gɛlɛya gɛlɛnw bɛ nɔ bila jago siratigɛ la ani kurunbokari siratigɛ la. Ni kɔkolo sirili dɔ tiɲɛna ka kɔn a waati ɲɛ, furakɛli bɛɛ tɛ se ka kɛ. Sariyasunba minnu bɛ i n’a fɔ FDA, olu bɛ sabatili kunnafoniw bɛɛ ɲini, minnu bɛ kɛ san caman kɔnɔ. Aw ka kan k’a jira ko kɔkolo bɛ to a cogo dafalen na waati tɛmɛnen kɔfɛ.
Jogoɲumanya kɔlɔsili bɛ gɛlɛyaba lase molekiyɔmuw dilanni ma. Aw ka kan ka sigicogo dafalen jira k’a jɛya sani aw ka baara kɛ. Laɲini molekiyɔrɔ ni sintezi kɔfɛfɛnw danfara ka gɛlɛn kosɛbɛ. Tɛmɛsira tigɛlenw bɛ fɛn laban ɲininen jira kosɛbɛ farikolo ta fan fɛ. Deletion nɔgɔw bɛ dogo nɔgɔya la kemikɛli ɲagaminen gɛlɛnw kɔnɔ. Jɛɲɔgɔnya kelen min tununna, o bɛ ɲɔgɔndan bɛɛ tiɲɛ. Aw mago bɛ sɛgɛsɛgɛli fɛɛrɛ barikamaw la, minnu kɛrɛnkɛrɛnnen don kosɛbɛ walasa ka ɲɛtaa sɛgɛsɛgɛ.
Sɛgɛsɛgɛli kɛcogo |
Baarakɛcogo fɔlɔ |
Laɲiniw tiɲɛni Paramɛtiri |
Bɔli cogoya |
|---|---|---|---|
HPLC (HPLC) ye |
Kɔrɔmatɔni (Chromatographie) farali ɲɔgɔn kan |
Kuma bɛɛ lajɛlen ka tugu-tugu ɲɔgɔn kɔ saniya |
Chromatogramme (kromatogramme) min bɛ kɛ a kuncɛ la |
Mass Spectrométrie (Mass Spectrométrie) ye |
Ion Massa Sumanikɛlan |
Molecules girinya tigitigi |
Masa-ka-sara-sɛbɛn |
NMR Spectroskopi (NMR) ye |
Ja min bɛ kɛ ni magɛnni ye |
Filanan sigicogo dafalen |
Kεmεkεli bεε bε Yεlεma |
FTIR Spectroskopi (Spektroskopi) ye |
Infrarouge yeelen minɛcogo |
Amide bond (amide bond) sɔrɔli |
Absorption (sɔgɔli) bɛ Kɛ a dan na |
High-Performance Liquid Chromatography (HPLC) tora diɲɛ bɛɛ ka sanu sariya ye. A bɛ fɛnɲɛnamafagalanw Bɔ ɲɔgɔn na ka Da ji-sɔgɔli kan pewu. HPLC bɛ laɲini dafalen bɔ nɔgɔ tigɛlenw na minnu ka surun. A bɛ saniya kɛmɛsarada la jɛlen ni kuntilenna di i ma. Mass Spectrometry (MS) bɛ molekiyɔmu girinya tigitigi sɛgɛsɛgɛ k’a ɲɛsin a yɛrɛ ma. ESI ni MALDI-TOF bɛ fɛɛrɛw jira minnu bɛ kɛ ka caya. MS bɛ ɲɔgɔndan dantigɛli nafama di o yɔrɔnin bɛɛ. A bɛ a to asidi aminikiw bɛɛ ka siri ɲɔgɔn na ka ɲɛ sɛnɛfɛnw dilanni waati. A bɛ jamaba danfara misɛnninw ye minnu bɛ sɔrɔ lakanajɛkuluw tununnenw fɛ nɔgɔya la.
Nucléaire magnétique résonance (NMR) ani Fourier Transform Infrared Spectroscopy (FTIR) bɛ fɛnw tiɲɛ minnu ka dun. U bɛ amidi jɛɲɔgɔnya kɛrɛnkɛrɛnnen sɔrɔli Sɛmɛntiya k’a jɛya. U bɛ ɲininikɛlaw dɛmɛ u ka sigida filananw kariti kɛ ka ɲɛ. O baarakɛminɛnw bɛ alfa-helisi walima beta-sɛbɛnw Sɔrɔ minnu bɛ Sɔrɔ cakɛda kɔnɔ. Kunnafoni faamuyali nafa ka bon kosɛbɛ fɛnɲɛnamafagalan sannikɛlaw bolo. Ni aw bɛ fɛn dilannikɛla dɔ ka sɛgɛsɛgɛli sɛbɛn (CoA) lajɛ, aw bɛ a lajɛ kosɛbɛ. Aw bɛ HPLC chromatogrammes ɲini minnu bɛ se ka sɛgɛsɛgɛ ka jɛya, minnu bɛ kuncɛ nɔgɔlenw jira. Aw ye aw jija MS kunnafoniw bɛ bɛn teori jatebɔ ma tigitigi. CoAw minnu tɛ jɛya, olu bɛ taamasiɲɛw jira minnu bɛ se ka kɛ fɛn dilanni nafama na walima nɔgɔ dogolenw. Aw mago bɛ daliluw la minnu bɛ sɔrɔ fɛn dilannenw ka ɲumanya kan sani aw ka taa ɲɛ.
Kemikɛli dilanni sɛgɛsɛgɛli bɛ laboratuwari gɛlɛya juguw lase diɲɛ kɔnɔ. Sɛnɛfɛnw sɔrɔta bɛ dɔgɔya kosɛbɛ ɲɔgɔndan janw na minnu tɛ kelen ye. Jɛɲɔgɔnya dɛsɛw bɛ juguya joona ni asidi aminikiw bɛɛ farala a kan. Jatebla lakika min bɛ sɔrɔ sinsinni ɲɔgɔn kɔ, o tɛ yafa.
Ni 99% ye ɲɔgɔndan nafa ye sen kelen na, o bɛ saniya 60% ɲɔgɔn di senfɛ 50 kɔnɔ.
Ni 95% ye baarakɛcogo ɲuman ye, o bɛ jigin ka kɛ ɲɔgɔndan bɛɛ lajɛlen sɔrɔta ye ka se 7% dɔrɔn ma.
Dɛsɛ cakɛda tigɛlenw bɛ kɛ fɛn ye min tɛ se ka faranfasi ka bɔ laɲini masala laban na kemikɛli siratigɛ la.
Saniya musakaw bɛ bonya kosɛbɛ ni ɲɔgɔndan janya bɛ bonya ka tɛmɛ tolen 30 kan.
Steric hindrance bɛ gɛlɛya ka t’a fɛ synthesis cɛsiribaw la. Kɛrɛfɛ cakɛda belebelebaw bɛ farikoloɲɛnajɛ kɛyɔrɔ bali farikolo la. Jɛɲɔgɔnya bɛ Kɛ tuma caman na ɲɔgɔndanw na minnu bɛ ji-dɛsɛ kosɛbɛ. Kemikɛli cakɛda min bɛ ka bonya, o bɛ foli kɔfɛ a yɛrɛ kan. O foli in bɛ amino kulu min bɛ se ka kɛ, o dogo pewu. Reactifs de couplage standard tɛ se ka don mass aggregated dense kɔnɔ. Walasa ka se sɔrɔ o kan, furakɛlikɛlaw bɛ baara kɛ ni fɛnɲɛnamafagalan kɛrɛnkɛrɛnnenw ye minnu bɛ kɛ ni fɛnɲɛnamaw ye. U bɛ fɛn barikamaw don minnu bɛ fɛnw ɲagami ɲɔgɔn na walasa ka idɔrɔzi jɛɲɔgɔnya tiɲɛ. Tuma dɔw la, u bɛ mikro-onde (mikɔrɔ-onde) funteni min bɛ kɔrɔsi, o kɛ walasa ka cakɛdaw wajibiya ka bɔ ɲɔgɔn na. Tuma caman na, ɲininikɛlaw bɛ pseudo-prolines (porolini pseudo-prolines) bila u nɔ na walasa ka o foli in bali ka kɔn a waati ɲɛ pewu.
Sɛgɛsɛgɛli fana bɛ wariko latigɛ gɛlɛnw wajibiya, minnu bɛ se ka kɛ. I bɛ musaka ni saniya jagokɛɲɔgɔnya kɛ tuma bɛɛ don o don. Walasa ka saniya 99% sɔrɔ min ka bon kosɛbɛ, o bɛ HPLC saniya boli caman de wajibiya. O kɛcogo kɛrɛnkɛrɛnnen bɛ furaji sɔngɔ gɛlɛnw caman dun, farati bɛ minnu na. A bɛ fɛnɲɛnamafagalanw sɔrɔta caman fana saraka farali waati. Kɛnɛyaso kɔnɔ furakɛli minnu bɛ kɛ in vivo, olu bɛ o saniya gɛlɛn in de wajibiya k’a sɔrɔ a ma kɛ ni sariya ye. O ni ɲinini kɛcogo jɔnjɔn minnu bɛ kɛ in vitro, olu bɛ se ka saniya 85% muɲu lafiya la. Aw ka kan ka wariko tiɲɛkow balansi ni timinandiya ye ni kɔrɔbɔli wajibiyalenw gɛlɛnw ye. Saniya hakɛ jugu sugandili bɛ baarakɛnafolo tiɲɛ ani ka kunnafonidilan nafamaw tiɲɛ.
Ka bɔ hakilinata la ka taa jago dafalen na, o bɛ jɛɲɔgɔnya fɛɛrɛw de wajibiya. Aw ka kan ka fɛɛrɛko sekow jateminɛ kosɛbɛ sani aw ka bolonɔ bila bɛnkansɛbɛnw na. Yala feerekɛla sugandilen bɛ se ka ɲɔgɔndan gɛlɛnw ɲɛnabɔ minnu ka gɛlɛn kosɛbɛ wa? Aw bɛ dɔnniya sɛgɛsɛgɛlen ɲini asidi aminikiw kan minnu tɛ u yɛrɛ ta ye. Aw ye u farikolo seko lajɛ walasa ka cyclique structures constrained (sɛrɛkiliw) labɛn. Aw bɛ a dɔn ni u bɛ baara kɛ ni fasa solide walima fasa jilama fɛɛrɛw ye fɔlɔ. Tuma caman na, LPPS bɛ baara kɛ ka ɲɛ jagokɛyɔrɔw la minnu ka surun kosɛbɛ, minnu hakɛ ka ca. SPPS bɛ ɲinini kɛcogo janw ɲɛnabɔ ka ɲɛ, minnu ka gɛlɛn kosɛbɛ. Feerekɛla ka kan k’u ka fɛɛrɛ kuraw bɛn i ka mɔlikulu kɛrɛnkɛrɛnnen ma.
Jogoɲumanya sabatili bɛ jɛɲɔgɔn tɔgɔbatigiw faranfasi ka bɔ sintezi laboratuwari jɔnjɔnw na. Sɛgɛsɛgɛli-dafalen dilanni bɛ danfaraba don ni cGMP labatoli gɛlɛn ye. Sisan, fɛn dilanni kɛcogo ɲumanw bɛ sigida kɔlɔsili gɛlɛnw de wajibiya. Feerekɛla ka kan ka baarakɛcogo jɔnjɔnw (SOP) sɛbɛnw mara tuma bɛɛ. U mago bɛ saniyaso kɛrɛnkɛrɛnnenw na, minnu kɔlɔsira kosɛbɛ, walasa ka baara kɛ. Lamini fiɲɛ hakɛ kɔlɔsili ka kan ka kɛ tuma bɛɛ k’a sɔrɔ a ma tigɛ. Aw ka kan ka jatebɔ sɛbɛnw ɲini minnu bɛ u ka yɔrɔw labatoli sariyaw ɲɛfɔ ka ɲɛ.
Donanw jɛlenya ye fɛn ye min tɛ se ka fɔ sannikɛla ŋanaw fɛ fewu. Aw ye dantigɛli jigiyaw sigi sen kan barow daminɛ na. Demand raw analytical reporting min bɛ di ni batch kelen-kelen bɛɛ ye. HPLC taamasiyɛnw ni MS spectres minnu bɛ se ka sɛgɛsɛgɛ, olu ye wajibi ye kosɛbɛ. Aw bɛ se ka tugu-tugu ɲɔgɔn kɔ dantigɛli kunnafoniw bɛɛ lajɛlen ɲini ka kɔn o ɲɛ. Ni feerekɛla dɔ bɛ sigasiga ka kunnafonidilan raw tila ɲɔgɔn na, i ka taa o yɔrɔnin bɛɛ. A ka c’a la, a bɛ sigida ka jogoɲini dɛsɛw jira.
Aw bɛ faratiw dɔgɔyali fɛɛrɛw ni fɛn caman tigɛli waatiw jateminɛ. Sintezi dɛsɛw bɛ Kɛ tuma caman na chimie complexe (kimisɛ) gɛlɛnw na. Jɛɲɔgɔn ɲumanba dɔ bɛ o fɛɛrɛko koɲɛw fɔ o yɔrɔnin bɛɛ. U bɛ coupling steps gɛlɛnw ɲɛnabɔ ka ɲɛ ni proactively ye k’a sɔrɔ u ma prompting kɛ. U bɛ kɔnɔna protocolw ladilan ni fanga ye walasa ka poroze waatibolodacogo gɛlɛnw dafa. U b’o kɛ k’a sɔrɔ u ma fɛn laban saniya saraka abada. Feerekɛlaw ka tariku barikama bɛ se ka fɛn dilanni dannaya nataw fɔ ka ɲɛ. Aw mago bɛ jɛɲɔgɔn na min bɛ kumaɲɔgɔnya kɛ kosɛbɛ, min bɛ kemikɛli gɛlɛyaw ɲɛnabɔ ni dɔnniya ye.
Pepitiri jɛɲɔgɔnya ka chimie jɔnjɔn dɔnni ye daminɛ dɔrɔn de ye. Ka o chimie nɔgɔlen baara ka kɛ fɛn dilanni ye min bɛ se ka da a kan, min bɛ se ka sɛgɛsɛgɛ, o bɛ jago gɛlɛya lakika jira. An ye sɛgɛsɛgɛli kɛ cogo min na jɔli gɛlɛya bɛ sabatili ni ɲɛnamaya baara fila bɛɛ fɔ. An y’a sɛgɛsɛgɛ mun na sinsinni kɛli ɲɔgɔn kɔ bɛ jatebɔ sɔrɔta dɔgɔyali don senkan min tɛ se ka bali.
Sɛgɛsɛgɛli barikama bɛ to i ka lafasali barikama ye ka ɲɛsin tiɲɛni ni sinsinni dɛsɛ ma. Feerekɛlaw ka jɛɲɔgɔnya fɛɛrɛw bɛ faratiba minnu bɛ sɔrɔ kemikɛli sɛgɛsɛgɛli la, olu nɔgɔya. I n’a fɔ fɛɛrɛ sannikɛla walima ɲininikɛla, i ka kan ka jɛlenya dafalen ɲini. Aw ye aw ka sisan sinsinnanw jateminɛ ka kɛɲɛ ni bi jateminɛ sariyaw ye. Aw bɛ fɛɛrɛko lajɛ caman ɲini ni ɲɔgɔndan o ɲɔgɔndan tɛmɛna tolen bisaba kan. Aw ye misali CoAw lajɛ kosɛbɛ sani aw ka aw yɛrɛ bila bɛnkanbaw la minnu bɛ kɛ ka kɛɲɛ ni mɔgɔw sago ye.
A: Hali ni pepitiri jɛɲɔgɔnyaw bɛɛ ye amidi jɛɲɔgɔnyaw ye fɛɛrɛko siratigɛ la, u bɛ yen kɛrɛnkɛrɛnnenya la asidi alfa-aminow ka ɲɛnamaya kɔnɔ. U bɛ asidi aminiki dɔ ka alfa-karɔkisili kulu ni dɔ wɛrɛ ka alfa-amino kulu siri ɲɔgɔn na. O biologiki labɛn kɛrɛnkɛrɛnnen in bɛ rezɔn sabatili kɛrɛnkɛrɛnnen Dabɔ, ka jateminɛ gɛlɛn, planar wajibiya min nafa ka bon poroteyini foli la.
A: Jiko ye jirisunw tiɲɛni sababu fɔlɔ ye. Ji bɛ kɛ i n’a fɔ fɛn min bɛ kɛ sababu ye, ka kovalɛn jɛɲɔgɔnya tigɛ. O wale in bɛ teliya kosɛbɛ pH jiginni kojugu waatiw la, ni a bɛ funteni caman na, walima ni banakisɛfagalanw nɔgɔli kasaara la, o bɛ poroteyolitiki anzimuw don a marayɔrɔ la minnu ma labɛn.
A: Sinsinnan bɛ farali de wajibiya ɲɔgɔn kɔ. Ni ɲɔgɔndan taabolo bɛɛ bɛ baara kɛ ni 99% ye, 1% dɛsɛ hakɛ bɛ fara ɲɔgɔn kan jatebɔ siratigɛ la. Ni sen 50 tugura ɲɔgɔn na, sɔrɔ bɛɛ lajɛlen bɛ jigin ka se 60% ɲɔgɔn ma. Stɛriki balili ni cakɛda farali bɛ yɔrɔw bali ka taa a fɛ minnu bɛ baara kɛ, o bɛ a to ɲɔgɔndan kɛcogo dafabaliw ka kɛ ni cakɛda bɛ janya.
A: I ka kan ka Sɛgɛsɛgɛli Sɛbɛn dɔ ɲini min kɔnɔ, sɛgɛsɛgɛli rapɔɔri fila-fila bɛ sɔrɔ. Aw bɛ HPLC chromatogrammew ɲini minnu ka bon kosɛbɛ walasa ka sample saniya bɛɛ lajɛlen jira ani ka nɔgɔ tigɛlenw dɔn. O waati kelen na, aw bɛ Mass Spectrometry (MS) kunnafoniw ɲini walasa ka molekiyɔmu girinya tigitigi sɛgɛsɛgɛ, ka sinsinni kɛcogo ɲuman sabati ani ka lakana kulu bɔli kɛcogo ɲuman sabati.
