TB4 10 mg

▎ TB4 Structure

Source: PubChem

Sequence: LKKTETQ Molecular Formula: C 38H 68N 10O14 Molecular Luj: 889.0 g / mol CAS Nr .: 885340-08-9 PubChem CID: 62707662 Synonyms: QHK6Z47GTG

▎ TB4 Kev Tshawb Fawb

Kev tshawb fawb keeb kwm ntawm TB4 yog dab tsi?

TB4 yog ib qho me me peptide ua tiav los ntawm qhov chaw nquag ntawm thymosin β4. Thymosin β4 muaj peev xwm ntawm cov ntaub so ntswg rov tsim dua tshiab, tiv thaiv kab mob, thiab kho sai, thiab TB4 tau txais cov khoom zoo li no. Thaum xub thawj, hauv kev tshawb fawb ntawm thymosin β4, nws tau pom tias muaj ntau yam kev ua haujlwm lom neeg, ua lub luag haujlwm tseem ceeb hauv cov haujlwm xws li kev hloov pauv ntawm tes, kho cov ntaub so ntswg, thiab kev tswj kev mob. TB4 yog cov khoom tawg yooj yim ntawm thymosin β4. Cov kws tshawb fawb vam tias los ntawm kev kawm txog TB4, lawv tuaj yeem nkag siab tob txog nws cov txheej txheem ntawm kev ua haujlwm thiab tshawb xyuas seb nws puas tuaj yeem tsim los rau hauv cov tshuaj uas muaj cov hom phiaj kho mob tshwj xeeb.

Hauv kev kho qhov txhab thiab cov ntaub so ntswg puas los ntawm cov kab mob ntev, txoj kev kho ib txwm muaj qee yam kev txwv. Vim nws lub peev xwm muaj peev xwm los txhawb kev hloov pauv ntawm tes thiab kho cov ntaub so ntswg, TB4 tau dhau los ua qhov kev tshawb fawb hotspot, thiab tib neeg cia siab tias nws yuav muab cov tswv yim tshiab thiab txoj hauv kev los kho cov kab mob no. Piv txwv li, hauv kev tshawb fawb ntawm cov kab mob xws li myocardial infarction thiab paj hlwb raug mob, kev tshawb fawb tau ua los tshawb xyuas seb TB4 puas tuaj yeem txhawb kev kho cov ntaub so ntswg puas thiab kho lawv cov haujlwm.

Cov neeg ncaws pob muaj kev raug mob ntau yam thaum lub sij hawm kev cob qhia thiab kev sib tw, nrog rau cov leeg nqaij thiab ligament raug mob. TB4 ntseeg tau tias muaj peev xwm pab kho qhov raug mob thiab txhim kho kev rov qab sai ntawm kev raug mob kis las, yog li nws tau nyiam cov tshuaj hauv kev ua kis las. Qee qhov kev tshawb fawb sim tshawb nrhiav cov peev txheej ntawm TB4 hauv kev kho mob ntawm cov neeg ncaws pob raug mob. Txawm li cas los xij, tib lub sijhawm, nws kuj tau ua rau muaj kev tsis sib haum xeeb txog seb nws puas tuaj yeem raug tsim txom raws li kev doping. Nrog rau txoj kev loj hlob ntawm cov tshuaj, qhov kev thov rau cov tshuaj tshiab yog tas li nce. Raws li cov tshuaj peptide nrog cov txheej txheem tshwj xeeb ntawm kev ua, TB4 muaj peev xwm tsim tau rau hauv hom tshuaj tshiab, muab kev xaiv ntau dua rau kev kho mob.

Yuav ua li cas yog lub mechanism ntawm kev txiav txim ntawm TB4?

Txhawb cov ntaub so ntswg regeneration: 

TB4 yog ib qho me me peptide ua tiav los ntawm qhov chaw nquag ntawm thymosin β4. Thymosin β4 muaj peev xwm txhawb kev tsim cov ntaub so ntswg, thiab TB4 tau txais cov cuab yeej no. Nws tuaj yeem txhawb kev tsim cov ntaub so ntswg hauv cov hauv qab no:

Activating cell signaling pathways: 

Nws tuaj yeem qhib qee cov xov tooj ntawm tes taw qhia txoj hauv kev los txhawb kev loj hlob ntawm tes thiab kev sib txawv. Piv txwv li, nws tuaj yeem qhib txoj hauv kev qhia txog kev loj hlob ntawm tes thiab kho, xws li PI3K / Akt signaling pathway, thiab lwm yam, yog li txhawb kev loj hlob ntawm tes thiab sib txawv thiab txhawb kev tsim cov ntaub so ntswg ^[1].

Regulating lub extracellular matrix: 

Extracellular matrix plays lub luag haujlwm tseem ceeb hauv kev tsim cov ntaub so ntswg. TB4 tuaj yeem tswj cov synthesis thiab degradation ntawm extracellular matrix, txhawb nqa cell adhesion, tsiv teb tsaws, thiab kho cov ntaub so ntswg. Piv txwv li, nws yuav ua rau kom cov synthesis ntawm collagen thiab elastin, txhim kho cov qauv thiab kev ua haujlwm ntawm cov ntaub so ntswg ^[1].

Anti-inflammatory nyhuv: 

Kev mob yog ib qho kev tiv thaiv ntawm lub cev rau kev raug mob thiab kis kab mob, tab sis kev mob ntau dhau tuaj yeem ua rau cov ntaub so ntswg puas. TB4 muaj cov tshuaj tiv thaiv kab mob thiab tuaj yeem tiv thaiv kev tsim cov inflammatory mediators. Inflammatory mediators xws li cytokines thiab chemokines ua lub luag haujlwm tseem ceeb hauv cov lus teb inflammatory. TB4 tuaj yeem cuam tshuam kev tsim cov tshuaj tiv thaiv kab mob, yog li txo cov lus teb inflammatory. Piv txwv li, nws yuav inhibit qhov tsim ntawm inflammatory yam xws li qog necrosis factor-α (TNF-α) thiab interleukin-1β (IL-1β) ^[1].

Regulating kev ua haujlwm ntawm lub cev tiv thaiv kab mob: 

Lub cev tiv thaiv kab mob ua lub luag haujlwm tseem ceeb hauv cov lus teb inflammatory. TB4 tuaj yeem tswj hwm kev ua haujlwm ntawm lub cev tiv thaiv kab mob, xws li tswj kev ua haujlwm ntawm macrophages thiab lymphocytes, yog li txo qis cov lus teb. Piv txwv li, nws tuaj yeem txhawb kev hloov pauv ntawm macrophages mus rau hauv cov tshuaj tiv thaiv kab mob phenotype thiab inhibit qhov ua kom thiab loj hlob ntawm lymphocytes ^[1].

Accelerating cell proliferation thiab sib txawv: 

Los ntawm activating cell signaling pathways thiab tswj cov extracellular matrix, TB4 tuaj yeem ua kom cov cell proliferation thiab sib txawv, txhawb kev kho cov ntaub so ntswg puas ^[1].

Txo cov inflammatory teb: 

Cov lus teb inflammatory yuav ncua cov ntaub so ntswg kho, thiab cov nyhuv anti-inflammatory ntawm TB4 tuaj yeem txo cov lus teb inflammatory, tsim kom muaj ib puag ncig zoo rau cov ntaub so ntswg kho ^[1].

Txhawb nqa angiogenesis: 

Angiogenesis yog qhov tseem ceeb rau kev kho cov ntaub so ntswg. TB4 tuaj yeem txhawb nqa angiogenesis, ua kom cov ntshav txaus rau cov ntaub so ntswg puas, muab cov as-ham thiab oxygen rau cov hlwb, thiab txhawb cov ntaub so ntswg kho ^[1].

Kev tswj hwm ntawm MMP / TIMP ntawm kab mob siab fibrosis.

Source: PubMed ^[3]

TB4 tswj cov synthesis thiab degradation ntawm extracellular matrix li cas?

Qhov sib npaug ntawm cov synthesis thiab degradation ntawm extracellular matrix (ECM) yog qhov tseem ceeb rau kev tswj cov qauv thiab kev ua haujlwm ntawm cov ntaub so ntswg. TB-500 tuaj yeem cuam tshuam cov synthesis ntawm extracellular matrix raws li hauv qab no:

Txhawb nqa collagen deposition: 

TB-500 ntseeg tau tias muaj peev xwm txhawb nqa collagen deposition, thiab collagen yog ib qho tseem ceeb ntawm cov extracellular matrix. Cov txheej txheem tshwj xeeb ntawm kev ua haujlwm yuav suav nrog kev tswj hwm ntawm tes taw qhia txoj hauv kev koom nrog hauv collagen synthesis. Piv txwv li, nws tuaj yeem txhawb nqa kev qhia ntawm collagen noob los ntawm kev ua kom muaj qee yam kev loj hlob lossis kev hloov pauv, yog li ua kom cov synthesis ntawm collagen ^[2].

Txhawb nqa endothelial cell sib txawv thiab angiogenesis: 

Cov hlwb endothelial secrete ntau yam ntawm cov khoom siv hauv matrix thaum lub sijhawm tsim cov hlab ntsha. TB-500 txhawb nqa endothelial cell sib txawv thiab angiogenesis nyob rau hauv cov ntaub so ntswg dermal, uas yuav indirectly txhawb lub synthesis ntawm extracellular matrix. Cov hlab ntsha uas tau tsim tshiab yuav tsum tau txais kev txhawb nqa ntawm extracellular matrix, uas tuaj yeem txhawb cov hlwb los ua ke ntau cov khoom siv hauv matrix extracellular ^[2].

Kev cuam tshuam ntawm degradation ntawm extracellular matrix:

Nws tuaj yeem tswj hwm cov haujlwm ntawm matrix metalloproteinases (MMPs) thiab lawv cov inhibitors (TIMPs): 

Lub degradation ntawm extracellular matrix feem ntau yog tswj los ntawm matrix metalloproteinases thiab lawv cov inhibitors. Txawm hais tias tam sim no tsis muaj pov thawj ncaj qha tias TB-500 tswj hwm cov dej num ntawm MMPs thiab TIMPs, xav tias TB-500 muaj kev cuam tshuam los ntawm kev txhawb nqa cell tsiv teb tsaws thiab kho qhov txhab, thiab cov txheej txheem ntawm cell tsiv teb tsaws thiab kho qhov txhab feem ntau yog nrog los ntawm kev hloov kho ntawm extracellular matrix. Piv txwv li, hauv kev tshawb fawb ntawm daim siab fibrosis, matrix metalloproteinases thiab lawv cov inhibitors tshwj xeeb (piv txwv li, cov ntaub so ntswg inhibitors ntawm metalloproteinases, TIMPs) ua lub luag haujlwm tseem ceeb hauv collagen synthesis thiab dissolution. Los ntawm kev rov ua kom qhov sib npaug ntawm MMPs thiab TIMPs, tsub zuj zuj ntawm extracellular matrix tuaj yeem inhibited, yog li txo cov mob siab fibrosis ^[3].

Indirectly tswj qhov degradation ntawm extracellular matrix los ntawm kev cuam tshuam cov cwj pwm ntawm tes: 

TB-500 tuaj yeem txhawb keratinocyte tsiv teb tsaws. Thaum lub sij hawm tus txheej txheem ntawm cell migration, hlwb yuav tsum tau tswj lub degradation ntawm lub extracellular matrix kom tshem tawm txoj kev. Qhov no tej zaum yuav koom nrog kev tso tawm ntawm qee cov enzymes los yog cov yam ntxwv los ntawm cov hlwb los tswj cov degradation ntawm extracellular matrix. Piv txwv li, nyob rau hauv ib co physiological thiab pathological txheej txheem, hlwb secrete matrix metalloproteinases kom degrade lub extracellular matrix rau kev tsiv teb tsaws ^[2].

Hauv txoj kev twg TB4 cuam tshuam nrog biomaterials los txhawb cov leeg nqaij?

Kev tso tawm ntawm bioactive molecules: 

Biomaterials tuaj yeem ua tus nqa khoom thiab ua haujlwm hauv kev hais kwv txhiaj nrog TB4 tso tawm cov khoom siv bioactive, txhawb cov leeg nqaij. Piv txwv li, qee cov khoom siv biomaterials tuaj yeem tso cov tshuaj nquag xws li kev loj hlob. Cov tshuaj no ua haujlwm ua ke nrog TB4 los txhawb kev loj hlob thiab sib txawv ntawm cov leeg hlwb. TB4 nws tus kheej muaj qhov cuam tshuam ntawm kev txhawb nqa cell migration thiab angiogenesis. Ua ke nrog cov nquag molecules tso tawm los ntawm biomaterials, nws muaj peev xwm ua tau zoo dua txhawb cov leeg nqaij ^{[4, 5]}.

Lub luag haujlwm ntawm cov khoom siv biomimetic: 

Cov ntaub ntawv biomimetic ua raws li cov qauv ntuj tsim thiab kev ua haujlwm ntawm cov leeg nqaij, muab qhov tsim nyog microenvironment rau TB4. Xws li cov ntaub ntawv biomimetic tuaj yeem ua tau zoo dua nrog cov leeg nqaij, txhawb kev ua haujlwm ntawm TB4 ntawm qhov chaw puas. Piv txwv li, cov ntaub ntawv biomimetic nrog cov qauv pore tshwj xeeb tuaj yeem muab kev txhawb nqa rau kev loj hlob ntawm tes, thiab tib lub sijhawm, cia TB4 diffuse thiab ua haujlwm zoo dua ^[4].

Immunomodulatory nyhuv: 

Biomaterials tuaj yeem txhawb nqa cov leeg nqaij los ntawm kev tswj hwm lub cev tiv thaiv kab mob, hauv kev koom tes nrog TB4. Cov kev tshawb fawb tau pom tias biomaterials tuaj yeem tswj cov polarization ntawm macrophages, yog li tswj lub cev tiv thaiv kab mob thiab tsim kom muaj ib puag ncig zoo rau cov leeg nqaij. TB4 tuaj yeem txhim kho cov nyhuv immunomodulatory ntxiv los ntawm kev cuam tshuam rau kev ua haujlwm ntawm lub cev tiv thaiv kab mob. Piv txwv li, los ntawm immunomodulation kho los ntawm biomaterials, polarization ntawm macrophages tuaj yeem tswj tau los txhawb cov nqaij mos rov tsim dua tshiab ntawm cov musculoskeletal system, thiab TB4 tuaj yeem ua lub luag haujlwm sib koom ua ke hauv cov txheej txheem no ^[5].

Kev sib xyaw ntawm cov qia hlwb thiab biomaterials: 

Stem cells ua lub luag haujlwm tseem ceeb hauv kev tsim cov leeg nqaij. Kev sib xyaw nrog biomaterials thiab TB4 tuaj yeem muab kev kho mob zoo dua. Ntau cov qia hlwb, xws li mesenchymal qia hlwb thiab adipose-derived qia hlwb, koom nrog hauv cov leeg nqaij. Biomaterials tuaj yeem muab kev txhawb nqa thiab kev taw qhia rau cov qia hlwb, thaum TB4 tuaj yeem txhawb nqa kev tsiv teb tsaws, kev ciaj sia, thiab kev sib txawv ntawm cov qia hlwb. Kev sib xyaw ua ke ntawm peb tuaj yeem kov yeej cov kev txwv ntawm kev siv lawv ib leeg thiab txhawb cov leeg nqaij.

Kev txhawb nqa ntawm paj hlwb rov qab: 

Peripheral paj regeneration kuj plays lub luag haujlwm tseem ceeb hauv cov leeg nqaij. Biomaterials tuaj yeem muab cov qauv kev sib txuas los txhawb cov paj hlwb, thiab TB4 tuaj yeem txhawb nqa cov paj hlwb thiab cov leeg ua haujlwm rov qab los ntawm kev cuam tshuam cov noob qhia ntsig txog neurogenesis. Piv txwv li, qee cov kev tshawb fawb tau pom tias cov noob arrays cuam tshuam nrog neurogenesis yog upregulated, tawm tswv yim lub luag hauj lwm ntawm peripheral paj regeneration nyob rau hauv kev kho kom rov zoo ntawm cov leeg nqaij, thiab biomaterials thiab TB4 yuav koom ua ke txhawb txoj kev no ^[6].

Daim ntawv thov ntawm magnetically teb biomaterials: 

Tshiab magnetically teb biomaterials tuaj yeem txhim kho cov nqaij ntshiv los ntawm kev siv tshuaj thiab xa xov ntawm tes. TB4 tuaj yeem siv ua ke nrog cov khoom siv biomaterial los txhim kho kev kho cov leeg nqaij puas. Piv txwv li, ib qho biphasic hlau gel scaffold tuaj yeem siv los xa cov hlwb thiab cov yam ntxwv loj hlob, lub sijhawm meej hauv vivo los txhim kho cov leeg nqaij rov ua haujlwm thaum mob. TB4 tuaj yeem ua haujlwm sib koom ua ke nrog cov khoom siv biomaterial no txhawm rau txhawb nqa cov leeg nqaij ^[7].

Zuag qhia tag nrho, raws li ib qho me me peptide ua tiav los ntawm qhov chaw nquag ntawm thymosin β4, TB4 tau pom tias muaj peev xwm zoo tshaj plaws hauv kev tsim cov nqaij mos, tiv thaiv kab mob, thiab kho sai. Kev tshawb fawb tau pom tias nws tuaj yeem txhawb kev sib txawv ntawm cov cell endothelial, angiogenesis, thiab keratinocyte tsiv teb tsaws, thiab tseem tuaj yeem tswj cov synthesis thiab degradation ntawm extracellular matrix. Hauv kev kho cov leeg nqaij, TB4 tuaj yeem ua rau muaj kev cia siab tshiab rau kev kho mob kis las kis las los ntawm kev txhawb nqa kev loj hlob thiab kev sib txawv ntawm cov leeg qia hlwb, tswj cov kab mob inflammatory, thiab cuam tshuam nrog biomaterials. TB4 muaj peev xwm los ua ib qho tshuaj zoo rau kev kho mob ntawm cov nqaij mos thiab lwm yam kab mob.

Hais Txog Tus Sau

Cov ntaub ntawv hais saum toj no yog txhua yam tshawb fawb, kho thiab sau los ntawm Cocer Peptides.

Scientific Journal Author

Ye J yog tus kws tshawb fawb ntawm Zhejiang University thiab yog tus tswv cuab ntawm Tuam Tshoj Orthopedic Regenerative Medicine Group (CORMed). Nws cov kev tshawb fawb muaj xws li engineering, khoom siv science, automation thiab tswj tshuab, kev lag luam thiab kev lag luam, thiab cov txheej txheem lej hauv social sciences. Ye J tau koom tes nrog ntau lub tsev kawm ntawv thiab cov koom haum, xws li Opt Clearing Corp, CTC Holdings, University of Illinois Chicago, thiab Dalian Institute of Chemical Physics, CAS. Ye J tau teev nyob rau hauv kev siv ntawm cov ntaub ntawv pov thawj [5].

▎ Cov Lus Qhia Tseem Ceeb

[1] Rahaman K, Muresan A, Son J, et al. Kev txhim kho ntawm txoj kev tshuaj ntsuam xyuas rau TB-500 thiab nws cov metabolites los ntawm LC-MS/MS[M]. 2022.10.13140/RG.2.2.32176.02564 ib.

[2] Ho ENM, Kwok WH, Lau MY, et al. Doping tswj tsom xam ntawm TB-500, ib tug hluavtaws version ntawm ib cheeb tsam nquag ntawm thymosin β4, nyob rau hauv equine zis thiab ntshav los ntawm kua chromatography-mass spectrometry [J]. Phau ntawv Journal of Chromatography A, 2012,1265:57-69.DOI:10.1016/j.chroma.2012.09.043.

[3] Shan L, Wang F, Zhai D, et al. Matrix metalloproteinases induce extracellular matrix degradation los ntawm ntau txoj hauv kev los daws cov kab mob siab hepatic [J]. Biomedicine & Pharmacotherapy, 2023,161.DOI:10.1016/j.biopha.2023.114472.

[4] Carleton MM, Sefton M V. Txhawb kev kho endogenous ntawm cov leeg pob txha siv cov khoom siv rov ua dua tshiab[J]. Phau ntawv Journal of Biomedical Materials Research Part A, 2021,109(12):2720-2739.DOI:10.1002/jbm.a.37239.

[5] Ye J, Xie C, Wang C, et al. Txhawb nqa musculoskeletal system mos cov ntaub so ntswg regeneration los ntawm biomaterial-mediated modulation ntawm macrophage polarization [J]. Bioactive Materials, 2021,6(11):4096-4109.DOI:10.1016/j.bioactmat.2021.04.017.

[6] Roberts K, Kim JT, Huynh T, et al. Transcriptome profiling ntawm ib tug synergistic volumetric leeg tsis kho lub tswv yim[J]. Bmc Musculoskeletal Disorders, 2023,24(1).DOI:10.1186/s12891-023-06401-1.

[7] Cezar C A. Magnetically Responsive Biomaterials for Enhanced Skeletal Muscle Regeneration[M]. 2015.https://www.proquest.com/dissertations-theses/magnetically-responsive-biomaterials-enhanced/docview/1761573755/se-2.

Tag nrho cov kab lus thiab cov ntaub ntawv ntawm cov khoom lag luam muab rau ntawm lub vev xaib no tsuas yog rau cov ntaub ntawv tshaj tawm kev tshaj tawm thiab cov hom phiaj kev kawm.  

Cov khoom muab rau hauv lub vev xaib no yog npaj tshwj xeeb rau kev tshawb fawb hauv vitro. Kev tshawb fawb hauv vitro (Latin: * hauv iav *, lub ntsiab lus hauv iav) yog ua los ntawm tib neeg lub cev. Cov khoom no tsis yog tshuaj, tsis tau txais kev pom zoo los ntawm US Food and Drug Administration (FDA), thiab yuav tsum tsis txhob siv los tiv thaiv, kho, lossis kho txhua yam mob, kab mob, lossis mob. Nws raug txwv nruj raws li txoj cai los qhia cov khoom no rau hauv tib neeg lossis tsiaj lub cev hauv txhua daim ntawv.