▎ Cartalax Ŋuti Numekuku
Nukae nye Cartalax ƒe numekuku?
Alesi woke ɖe eŋu: Woke ɖe eŋu gbã le nyitsu ƒe ayiku ŋuti numekuku me. Polypeptide si woɖe tso ayiku me la te ŋu do ŋusẽ ayiku me lãmenugbagbeviwo ƒe yeyewɔwɔ le sisiblisi tsitsiwo me. To numekuku me la, wokpɔe be peptide vovovowo abe Cartalax (AED) kple T-35 (EDL) le nusi woɖe tso eme la me. Nu sia si ŋu woke ɖo la na dzɔdzɔmeŋutinunyalawo wɔ numekuku deto le Cartalax ƒe nɔnɔme kple dɔwɔwɔ ŋu.
Numekuku le alesi wòwɔa dɔe ŋu: Numekukuwo ɖee fia be Cartalax wɔa dɔ ɖe fibroblasts koŋ dzi. Fibroblasts le lãmekawo abe amegbetɔ ƒe ƒunukpeƒetetedɔ, ŋutigbalẽ, ʋukawo, kple ayiku me geɖe. Cartalax kpɔa ŋusẽ ɖe lãmenugbagbeviwo ƒe dzidziɖedzi kple woƒe tsɔtsrɔ̃ dzi to molecule ƒe mɔnu vovovowo dzi, abe lãmenugbagbeviwo ƒe dzidziɖedzi ƒe dzesi Ki-67 ƒe agbɔsɔsɔ dzi ɖeɖe kpɔtɔ, p53 ƒe dzesiwo dzi ɖeɖe kpɔtɔ, kple NF-κB ƒe dzesimɔ ƒe dɔwɔwɔ ene. Dɔwɔwɔ siawo naa Cartalax wɔa dɔ siwo nye lãmenugbagbeviwo ƒe tsitsi dzi ɖeɖe kpɔtɔ, lãmenugbagbevi tsitsiwo ƒe dɔwɔwɔ gbugbɔgaɖoanyi, kple lãmenugbagbeviwo ƒe tsitsi hehe ɖe megbe, eyata wobui be enye nugbagbewo ƒe ɖoɖowɔla si tsia tsitsi nu.
Dɔdamɔnu ŋuti numekuku: Le amegbetɔwo ƒe dodokpɔ siwo wowɔ le St. Petersburg Institute of Bioregulation and Gerontology me la, wozã Cartalax tsɔ da dɔ na dɔnɔ siwo ŋu ƒunukpeƒetetedɔ kple ƒumeŋuɖui le. Nusiwo do tso eme ɖee fia be Cartalax ate ŋu aɖe dɔnɔ siwo ŋu klo ƒe ƒunukpeƒetetedɔ le ƒe 68% kple dɔnɔ siwo ŋu ƒunukpeƒetetedɔ le ƒe 53% ƒe vevesese dzi akpɔtɔ, ana ƒunukpeƒetetedɔ nanyo ɖe edzi, eye wòaɖe ƒunukpeƒetetedɔ ƒe dzesiwo afia le nɔnɔmetatawo ɖeɖe me, eye viɖe aɖewo hã le eŋu na ƒumeŋuɖui ƒe dzesiwo.
Nukae nye Cartalax ƒe dɔwɔwɔ ƒe mɔnu?
Akpa si Cartalax wɔna le lipid micelle system ƒe oxidation me
Ŋusẽkpɔɖeamedzi ɖe oxidation ƒe dɔwɔwɔ dzi
Le in vitro ɖoɖo me la, Cartalax kpɔa ŋusẽ gã aɖe ɖe oxidation ƒe dɔwɔwɔ dzi. Le go ɖeka me la, le nɔnɔme aɖewo me la, ate ŋu axe mɔ ɖe Fe2+-si hena substrate oxidation process [1] . Oligopeptide la wɔa ŋusẽ sia le cationic initiator ƒe kadodo ta. Esia ɖee fia be Cartalax ate ŋu ado kplamatse oxidation ƒe dɔwɔwɔ ƒe gɔmedzedze to nuwɔwɔ kple cations me, si ana oxidation ƒe dɔwɔwɔ nagbɔdzɔ.
Ke hã, le oxidation nɔnɔme siwo azobis(isobutyronitrile) dze egɔme me la, Cartalax ana oxidation la nawɔ kabakaba. Le nɔnɔme siawo me la, le nusi wowɔ kple antioxidants me la, Cartalax wɔa dɔ abe mɔxenu ene kple peptide bubu siwo ƒe atikewo ƒe wɔwɔme to vovo, si ɖea β-carotene kple α-tocopherol ƒe mɔxexeɖedɔléle nu dzi kpɔtɔna ŋutɔ. Esia fia be le oxidation gɔmedzedze ƒe nɔnɔme tɔxɛwo me la, Cartalax ƒe anyinɔnɔ ate ŋu atrɔ antioxidant ƒe dɔwɔwɔ ƒe mɔnu, si ana eƒe mɔxexeɖedɔléle ƒe ŋusẽkpɔɖeamedzi ɖe oxidation dzi nagbɔdzɔ [1]..
Nuwɔwɔ aduadu kple nu bubuwo
Le lipid micelle system ƒe oxidation dɔwɔwɔ me la, Cartalax wɔa dɔ kple bioactive oligopeptide bubuwo, model dipeptides kple tripeptides, kple antioxidants. Le kpɔɖeŋu me, ne wole ɖeka le vitro ɖoɖo aɖe me kple nusiwo nye Veron (Lys-Glu), Vesugen (Lys-Glu-Asp), Pineal (Glu-γ-Asp-delta-Arg), honluten (Glu-I-3-Asp-Gly), Azi (Glu-I-3-Asp-Leu), kristagen (Glu-I-3-Asp-Pro), Epita (Ala-I-3-Glu-I-3-Asp-Gly), Carnosine (β-Ala-His), Glycylglycine (Gly-Gly), Glycylglycylglycine (Gly-Gly-Gly), Prolylproline (Pro-Pro), Prolylproline (Pro-Pro-Pro), kple Glutathione (gamma-Glu-Cys-Gly), eƒe oxidation ƒe nɔnɔmewo ɖea vovototo fiana [1] ..
Dodokpɔwo ɖee fia be ne peptidewo li la, hydroperoxides ƒe ƒuƒoƒo dzina ɖe edzi, eye antioxidants ƒe xɔxlɔ̃ ƒe agbɔsɔsɔme hã trɔna. Esia ɖee fia be menye ɖeko Cartalax kpɔa ŋusẽ ɖe oxidation ƒe dɔwɔwɔ ŋutɔ dzi ko o, ke ekpɔa ŋusẽ ɖe nu bubuwo ƒe ƒuƒoƒo kple woƒe xɔxlɔ̃ dzi le oxidation ƒe dɔwɔwɔ me hã.
Akpa si enzymes kple dzɔdzɔme oxidation inhibitors sɔsrɔ̃ wɔna
Cartalax ƒe wɔwɔme si wodo ɖa la srɔ̃a enzymewo kple dzɔdzɔme oxidation inhibitors ƒe antioxidant ŋusẽkpɔɖeamedzi le vitro, siwo le nugbagbe gbagbewo me [1] . Esia fia be Cartalax ate ŋu awɔ dɔ abe enzyme kple dzɔdzɔme antioxidants ene le nugbagbewo me eye wòate ŋu anye vevienyenye na nugbagbewo ƒe oxidation dadasɔ me léle ɖe asi.
Nukae nye Cartalax ƒe dɔwɔwɔ?
Ƒunukpeƒewo ɖɔɖɔɖo kple ƒunukpeƒewo ƒe lãmesẽ: Cartalax kpena ɖe ƒunukpeƒeŋusẽ ƒe lãkusiwo ƒe blibonyenye ŋu to protein ƒe wɔwɔme si le ƒunukpeƒewo me, si wɔnɛ be ƒunukpeƒewo ƒe vevesese kple sesẽ dzi ɖena kpɔtɔna eye wònaa ƒunukpeƒewo ƒe dɔwɔwɔ nyona ɖe edzi. Cartalax ɖee fia be ewɔa dɔ ɖedzesi aɖe le dɔlélewo abe ƒunukpeƒetetedɔ, ƒunukpeƒetetedɔ, kple ƒumeŋuɖui ene dada me. Ate ŋu ado ƒunukpeƒetetedɔ me protein veviwo abe kolagen ene ƒe wɔwɔ ɖe ŋgɔ, ado alɔ ƒunukpeƒea ƒe wɔwɔme ƒe blibonyenye, eye wòakpe ɖe eŋu wòahaya tso nusiwo gblẽ le eƒe vuvu alo dɔdzẽ ta me. Tsɔ kpe ɖe eŋu la, Cartalax kpɔa ŋusẽ ɖe ame tsitsiwo ƒe akɔta kple ƒunukpeƒewo ƒe abi siwo gblẽna hã dzi, si kpena ɖe ame tsitsiwo ƒe abi siawo ƒe dzɔdzɔ ɖe megbe eye wònaa ame tsitsiwo ƒe agbenɔnɔ nyona ɖe edzi [2] ..
Dɔlékuiwutike: Dɔlékuiwutike le Cartalax ŋu eye ate ŋu aɖɔ dɔdzẽ ƒe ŋuɖoɖo ɖo. Ekpɔa ŋusẽ ɖe cytokines ƒe wɔwɔ dzi to nuwɔwɔ kpli wo me eye wòganaa dɔdzẽ ƒe ŋuɖoɖo si da sɔ la gaɖoa wo me. Le dɔdzẽ si wodo ɖa ƒe kpɔɖeŋu me la, Cartalax ɖe pro-inflammatory cytokines (abe IL-1β, TNF-α, kple COX-2) ƒe agbɔsɔsɔ dzi kpɔtɔ, eye le ɣeyiɣi ma ke me la, edzi dɔlékuiwutike kple lãmenugbagbeviwo ɖɔɖɔɖomɔwo ƒe akpa veviwo abe IL-10 kple TGF-β ene ƒe ɖeɖefia ɖe edzi. Weintsetse ƒe nuku proanthocyanidin extract (GSPE) ate ŋu aɖe ŋutigbalẽ ƒe titrime ƒe dzidziɖedzi si tsoa dɔdzẽ gbɔ dzi akpɔtɔ eye wòaɖɔ ʋumenugbagbevi ɣiwo ƒe xexlẽme si to vovo le ʋumenugbagbeviɣi ƒe kpɔɖeŋuwo me ɖo. Ne wotsɔe sɔ kple atike si tsia dzodzodzoetsitsi nu si nye indomethacin la, GSPE ɖe dɔlékuiwutike nyuiwo fia (Radhi H, 2021). Dɔlékuiwutike sia na be asixɔxɔ si ate ŋu anɔ Cartalax ŋu le dɔdzẽ ƒe dɔléle vovovowo dada me, vevietɔ le ƒunukpeƒetetedɔ kple ƒunukpeƒetetedɔ bubuwo me [2] ..
Lãmetsiwo ƒe gbugbɔgadzɔ: Cartalax ate ŋu ado alɔ lãkusi vovovowo ƒe gbugbɔgadzɔ ƒe ŋutete to lãmenugbagbeviwo kple tɔgbui lãmenugbagbeviwo ƒe dzidziɖedzi dodo ɖe ŋgɔ me. Numekuku siwo wowɔ le vitro ɖee fia be Cartalax ate ŋu ana mesenchymal stem cells (MSCs) ƒe dzidziɖedzi ƒe agbɔsɔsɔ nadzi ɖe edzi, siwo le vevie ŋutɔ na lãmenugbagbeviwo dzadzraɖo kple wo dzi kpɔkpɔ elabena woate ŋu ato vovo azu lãmenugbagbevi ƒomevi tɔxɛ siwo hiã be woalé lãmenugbagbeviwo ƒe dɔwɔwɔ ɖe te. Nɔnɔme sia na be Cartalax ƒe dɔwɔwɔ ƒe mɔkpɔkpɔ keke ta le lãmenugbagbeviwo ƒe dɔwɔwɔ kple gbugbɔgawɔ ƒe atikewɔwɔ me, vevietɔ le ƒunukpeƒe kple ƒu ƒe lãkusiwo gbugbɔgawɔ me [2]..
Kamedede ƒe hayahaya: Cartalax wɔa dɔ le kamedefefewo ƒe atikewɔwɔ hã me, vevietɔ le kamedefefewɔlawo alo amesiwo ƒoa wo ɖokui ɖe kamedede sesẽwo me dome. Ate ŋu ado alɔ ƒunukpeƒewo ƒe lãmesẽ, aɖe ƒunukpeƒewo ƒe gbegblẽ si kamedede hena vɛ dzi akpɔtɔ, eye wòado ƒunukpeƒewo ɖɔɖɔɖo ɖe ŋgɔ. Le kamedefefewɔla siwo ƒoa wo ɖokui ɖe hehexɔxɔ sesẽ me zi geɖe gome la, Cartalax ate ŋu akpe ɖe wo ŋu woahaya kabakaba le hehexɔxɔ kple hoʋiʋliwo megbe eye wòaɖe ƒunukpeƒekuxi siwo dona tso eŋudɔwɔwɔ fũu akpa hena vɛ dzi akpɔtɔ.
Dɔléle siwo tsoa dɔlékuiwo ƒe dɔwɔwɔ me kple ɣeyiɣi si wowɔna le dɔwɔwɔ nɛ vɔ megbe: Wozãa cartalax hã le ƒunukpeƒetetedɔ ƒe dɔlélewo me kple le ɣeyiɣi siwo do ŋgɔ na dɔwɔwɔ na ame kple esiwo wowɔna le ƒunukpeƒewo ƒe dɔwɔwɔ vɔ megbe me. Le kadodoŋusẽ ƒe dɔlélewo me la, Cartalax kpena ɖe ame ŋu wòɖɔa dɔlélenutsiŋutete ƒe dɔwɔwɔ ɖo, eɖea dɔdzẽ dzi kpɔtɔna, eye wòkpɔa ƒunukpeƒewo kple ƒunukpeƒewo ta. Le ƒunukpeƒedɔwɔwɔ do ŋgɔ na dɔwɔwɔ kple dɔwɔwɔ vɔ megbe la, Cartalax ate ŋu akpe ɖe ame ŋu be wòaxe mɔ ɖe ƒu kple ƒunukpeƒewo ƒe gbegblẽ nu eye wòana ame nahaya le dɔwɔwɔ vɔ megbe, si aɖe dɔwɔwɔ vɔ megbe kuxiwo ƒe dzɔdzɔ dzi akpɔtɔ.
Mɔxexe ɖe abi siwo gblẽna le ame tsitsiwo ŋu nu: Le ame tsitsiwo gome la, Cartalax kpena ɖe ame ŋu be woaxe mɔ ɖe abi siwo gblẽna le akɔta kple ƒunukpeƒewo nu. Ne ame tsi la, ƒunukpeƒetetedɔ kple ƒunukpeƒeŋusẽ ƒe gbegblẽ le dzɔdzɔme nu nye nusi womate ŋu aƒo asa na godoo, gake Cartalax ate ŋu ahe nuwɔna sia ɖe megbe to ʋumenugbagbevi dzĩwo ƒe dɔwɔwɔ dodo ɖe ŋgɔ kple collagen ƒe wɔwɔme dodo ɖe ŋgɔ me, ƒunukpeƒewo ƒe dɔwɔwɔ nanyo ɖe edzi, eye ame tsitsiwo ƒe agbenɔnɔ nanyo ɖe edzi.
Ŋutilã ƒe lãmesẽ: Numekukuwo ɖee fia be Cartalax ate ŋu ana ŋutigbalẽa nate ŋu aʋã kple eƒe wɔwɔme nanyo ɖe edzi to collagen ƒe wɔwɔme dodo ɖe ŋgɔ alo ŋutigbalẽ ƒe lãmenugbagbeviwo ƒe dɔwɔwɔ nyuie wu me. Esia na be asixɔxɔ si ate ŋu anɔ Cartalax ŋu le ŋutigbalẽdzikpɔkpɔ kple tsitsi nutsitsi ƒe akpawo me, vevietɔ le ŋutigbalẽ ƒe nɔnɔme ƒe nyonyome kple flitete dzi ɖeɖe kpɔtɔ me [2]..
Ayikuwo takpɔkpɔ: Numekuku aɖewo ɖee fia be Cartalax ate ŋu akpɔ ŋusẽ ɖe ayiku ƒe dɔwɔwɔ kple eƒe wɔwɔme ta. Ate ŋu akpɔ ayikuwo ta tso nusiwo agblẽ le wo ŋu me to ŋusẽkpɔkpɔ ɖe lãmenugbagbeviwo ƒe dɔwɔwɔ le ayikuawo me dzi, si aɖe dɔdzẽ kple oxidative stress dzi akpɔtɔ. Ayiku takpɔnu sia na be asixɔxɔ si woate ŋu azã le Cartalax ŋu le ayikudɔlélewo kple dɔléle siwo do ƒome kplii dada me.
Tsitretsitsi ɖe tsitsi ŋu: Wobua cartalax be enye peptide si tsia tsitsi nu si ate ŋu ana tsitsi ƒe akpa aɖewo nagbɔdzɔ to ŋusẽkpɔkpɔ ɖe lãmenugbagbeviwo ƒe dɔwɔwɔ vovovowo dzi me. Ate ŋu ana ŋusẽ kple lãmesẽ bliboa nanyo ɖe edzi to lãmenugbagbeviwo ƒe tsatsam dzi kpɔkpɔ, oxidative stress kple inflammatory responses dzi ɖeɖe kpɔtɔ, kple lãmenugbagbeviwo ɖɔɖɔɖo kple gbugbɔgadzɔ dodo ɖe ŋgɔ me. Nɔnɔme sia si tsi tre ɖe tsitsi ŋu na be asixɔxɔ si ate ŋu azã Cartalax le dɔléle siwo do ƒome kple tsitsi hehe ɖe megbe kple lãmesẽ bliboa ƒe nyonyo me [2]..
Alesi Cartalax wɔa dɔe la sesẽ, eye wòkpɔa ŋusẽ eve ɖe oxidation ƒe dɔwɔwɔ dzi. Ate ŋu awɔ nu kple nu vovovowo eye wòawɔ abe antioxidant ƒe dɔwɔwɔwo ene. Eƒe zazã keke ta, si ɖe ŋutete si le eŋu fia le go geɖe abe lãmenugbagbeviwo ƒe gbugbɔgadzɔ, kamedefefewo ƒe hayahaya, dɔlélewo dada, kple ŋutigbalẽ ƒe lãmesẽ me.
Le Agbalẽŋlɔla La Ŋu
Nusiwo míeyɔ ɖe etame la katã nye esiwo ŋu Cocer Peptides wɔ numekuku le, trɔ asi le wo ŋu eye woƒo wo nu ƒu.
Dzɔdzɔmeŋutinunya ƒe Agbalẽŋlɔla
Storozhok NM nye numekula si do ƒome kple Tyumen Dukɔa ƒe Atikewɔwɔ Yunivɛsiti, N. N. Semenov Dukɔa ƒe Numekuku Dɔwɔƒe si Kpɔa Dzɔdzɔmeŋusẽŋununya ƒe Dzɔdzɔmeŋusẽŋununya le Russia Dzɔdzɔmeŋutinunya Suku, kple TYUMEN INST MED. Eƒe dɔa ku ɖe numekuku siwo tso nusɔsrɔ̃ vovovowo me le Dzɔdzɔmeŋusẽŋununya, Nu gbagbewo ƒe Dzɔdzɔmeŋusẽŋununya & Molecular Biology, Biophysics, Cell Biology, kple Physics me. Ewɔ akpa vevi aɖe le go siawo me to eƒe sukudede kple numekukudɔwo me. Woŋlɔ Storozhok NM ɖe nyayɔyɔ ƒe numekugbalẽ me [1].
