Research Ubushakashatsi bwa Cartalax
Ni ubuhe bushakashatsi bwakozwe na Cartalax?
Uburyo bwo kuvumbura: Byabanje kuvumburwa mugihe cyo kwiga impyiko. Igikoresho cya polypeptide gitandukanijwe nimpyiko cyashoboye gutera imbaraga zo kuvugurura ingirangingo zimpyiko zishaje. Binyuze mu isesengura, byagaragaye ko ibiyikubiyemo birimo peptide zitandukanye nka Cartalax (AED) na T-35 (EDL). Ubu buvumbuzi bwakuruye ubushakashatsi bwimbitse bwakozwe n'abahanga ku miterere n'imikorere ya Cartalax.
Ubushakashatsi ku buryo bwibikorwa: Ubushakashatsi bwerekanye ko Cartalax ikora cyane kuri fibroblast. Fibroblast igaragara cyane mubice nka karitsiye yabantu, uruhu, imiyoboro yamaraso, nimpyiko. Cartalax igira ingaruka ku ikwirakwizwa ry'uturemangingo na apoptose ikoresheje uburyo bwinshi bwa molekile, nko kongera urwego rw'ikimenyetso cyo gukwirakwiza selile Ki-67, kugabanya ibimenyetso bya p53, no gukora inzira ya NF-κB. Izi ngaruka ziha Cartalax imirimo yo kugabanya senescence, kugarura imikorere yingirabuzimafatizo zishaje, no gutinda gusaza kwinyama, bityo ikaba ishyirwa mubikorwa byo kurwanya ibinyabuzima birwanya gusaza.
Ubushakashatsi bwa Clinical: Mu bigeragezo byabantu byakorewe mu kigo cya St. Petersburg Institute of Bioregulation na Gerontology, Cartalax yakoreshejwe mu kuvura abarwayi barwaye osteoarthritis na osteoporose. Ibisubizo byerekanye ko Cartalax ishobora kugabanya ububabare bwa 68% by’abarwayi barwaye ivi osteoarthritis na 53% by’abarwayi barwaye spondylarthrosis, bagahindura urujya n'uruza, kandi bakerekana ibimenyetso byerekana ko karitsiye itera amashusho, kandi ikagira inyungu zimwe na zimwe ku bimenyetso bya osteoporose.
Ni ubuhe buryo bwo gukora bwa Cartalax?
Uruhare rwa Cartalax muri okiside ya sisitemu ya lipide micelle
Ingaruka kuri gahunda ya okiside
Muri sisitemu ya vitro, Cartalax igira ingaruka zikomeye kubikorwa bya okiside. Ku ruhande rumwe, mu bihe bimwe na bimwe, irashobora kubuza uburyo bwa okiside ya Fe2 + iterwa na substrate [1] . Oligopeptide ikora iyi ngaruka kubera guhuza intangiriro ya cationic. Ibi byerekana ko Cartalax ishobora kubangamira icyiciro cyo gutangiza reaction ya okiside ikorana na cations, bityo bikadindiza inzira ya okiside.
Ariko, mugihe cya okiside yatangijwe na azobis (isobutyronitrile), Cartalax izihutisha okiside. Muri ibi bihe, mubigize hamwe na antioxydants, Cartalax ikora nka antagonist hamwe nizindi peptide zububiko butandukanye bwimiti, bigabanya cyane ingaruka mbi za β-karotene na α-tocopherol. Ibi bivuze ko mugihe cyihariye cyo gutangiza okiside, kuba Cartalax ishobora guhindura uburyo bwibikorwa bya antioxydeant, bikagabanya ingaruka zayo zo kubuza okiside [1].
Imikoranire nibindi bintu
Mugihe cya okiside ya sisitemu ya lipide micelle, Cartalax ikorana nizindi oliopeptide bioactive, dipeptide ntangarugero na tripeptide, na antioxydants. Kurugero, iyo dufatanije muri sisitemu ya vitro hamwe nibintu nka Veron (Lys-Glu), Vesugen (Lys-Glu-Asp), Pineal (Glu-γ-Asp-delta-Arg), honluten (Glu-I-3-Asp-Gly), Amagi (Glu-I-3-Asp-Leu), Amagi (Glu-I-3-Asp-Leu) Epithalon (Ala-I-3-Glu-I-3-Asp-Gly), Carnosine (β-Ala-His), Glycylglycine (Gly-Gly), Glycylglycylglycine (Gly-Gly-Gly), Prolylproline (Pro-Pro), Prolylproline (gamma-Glu-Cys-Gly), imiterere ya okiside yerekana itandukaniro [1].
Ubushakashatsi bwerekanye ko imbere ya peptide, igipimo cyo gukusanya hydroperoxide cyiyongera, kandi igipimo cyo kwinjiza antioxydants nacyo kirahinduka. Ibi byerekana ko Cartalax itagira ingaruka kuri gahunda ya okiside yonyine ahubwo igira ingaruka no kwegeranya no kwinjiza ibindi bintu mugihe cya okiside.
Uruhare rwo kwigana imisemburo na inhibitori ya okiside
Icyifuzo cya Cartalax cyigana ingaruka za antioxydeant ya enzymes hamwe na inhibitori ya okiside isanzwe muri vitro, iba mu binyabuzima [1] . Ibi bivuze ko Cartalax ishobora kuba ifite imirimo isa niy'imisemburo na antioxydants karemano mu binyabuzima kandi bifite akamaro kanini mu gukomeza kuringaniza okiside y’ibinyabuzima.
Ni ubuhe buryo bukoreshwa muri Cartalax?
Gusana karitsiye hamwe nubuzima bufatanije: Cartalax ifasha kugarura ubusugire bwimitsi ya karitsiye itera poroteyine ya chondrocytes, bityo bikagabanya ububabare hamwe no gukomera no kunoza imikorere. Cartalax yerekanye ingaruka zikomeye mu kuvura indwara nka spondylarthrosis, osteoarthritis, na osteoporose. Irashobora guteza imbere synthesis ya proteine zingenzi za karitsiye nka kolagen, igashyigikira ubusugire bwimiterere ya matrise ya karitsiye, ikanayifasha gukira ibyangiritse biterwa no kwambara cyangwa gutwikwa. Byongeye kandi, Cartalax igira kandi ingaruka zo gukumira indwara zangirika z’umugongo hamwe n’ingingo ku bageze mu za bukuru, bifasha gutinda kubaho kw’ibi bisebe no kuzamura imibereho y’abasaza [2].
Ingaruka zo kurwanya inflammatory: Cartalax ifite imiti igabanya ubukana kandi irashobora kugenga igisubizo. Ihindura umusaruro wa cytokine muguhuza nabo kandi igarura igisubizo cyuzuye. Mu buryo bwo gutwikwa kwatewe, Cartalax yagabanije urugero rwa cytokine itera umuriro (nka IL-1β, TNF-α, na COX-2), kandi icyarimwe yongera imvugo yibice byingenzi bigize inzira zo gusana no gutwika imyenda nka IL-10 na TGF-β. Imbuto y'imizabibu proanthocyanidin ikuramo (GSPE) irashobora kugabanya kwiyongera k'ubugari bwuruhu rwatewe no gutwikwa no kugena itandukaniro ritandukanye rya selile yera mumaraso ya serumu yamaraso. Ugereranije n’imiti igabanya ubukana indomethacine, GSPE yerekanye ingaruka nziza zo kurwanya inflammatory (Radhi H, 2021). Izi ngaruka zo kurwanya inflammatory zituma Cartalax igira agaciro gakoreshwa mukuvura indwara zinyuranye, cyane cyane muri rubagimpande nizindi ndwara zifata ingingo [2].
Kuvugurura imyenda: Cartalax irashobora gushyigikira ubushobozi bwo kuvugurura ingirangingo zitandukanye mugukwirakwiza ingirabuzimafatizo na progenitor. Mu bushakashatsi bwa vitro bwerekanye ko Cartalax ishobora kongera umuvuduko ukabije w'ingirabuzimafatizo ya Mesenchymal stem selile (MSCs), zikaba ari ingenzi cyane mu gusana no gufata neza kuko zishobora gutandukana mu bwoko bw'utugingo ngengabuzima dusabwa kugira ngo dukomeze imikorere ya tissue. Ibi biranga bituma Cartalax ifite amahirwe menshi yo gukoreshwa mubijyanye nubuhanga bwimikorere nubuvuzi bushya, cyane cyane mu kuvugurura ingirabuzimafatizo hamwe n’amagufwa [2].
Imyitozo ngororamubiri: Cartalax ifite kandi ubuvuzi mubuvuzi bwa siporo, cyane cyane mubakinnyi cyangwa abantu bakora imyitozo ikomeye. Irashobora gushyigikira ubuzima buhuriweho, kugabanya kwangirika kwimyitozo ngororamubiri, no guteza imbere gusana karitsiye. Ku bakinnyi bakunze kwitabira imyitozo yimbaraga nyinshi, Cartalax irashobora kubafasha gukira vuba nyuma yimyitozo namarushanwa no kugabanya ibibazo bihuriweho biterwa no gukoresha cyane.
Indwara zifatika hamwe nigihe cya perioperative: Cartalax ikoreshwa no muburwayi bwa sisitemu ihuza indwara ndetse no mugihe cyo gutangira na nyuma yo kubagwa hamwe. Mu ndwara zifatika zifatika, Cartalax ifasha kugenzura ubudahangarwa bw'umubiri, kugabanya umuriro, no kurinda ingirangingo hamwe na karitsiye. Mugihe cyo gutangira na nyuma yibikorwa byo kubaga hamwe, Cartalax irashobora gufasha gukumira amagufwa no kwangirika hamwe no guteza imbere gukira nyuma yo kubagwa, bikagabanya ibibazo biterwa na nyuma yibikorwa.
Kwirinda ibisebe byangirika ku bageze mu zabukuru: Ku bageze mu zabukuru, Cartalax ifasha kwirinda ibikomere byangirika by'umugongo hamwe n'ingingo. Hamwe no kwiyongera kwimyaka, byanze bikunze kwangirika kwa karitsiye hamwe nuduce twose byanze bikunze, ariko Cartalax irashobora gutinza iki gikorwa mugukangurira ibikorwa bya chondrocytes no guteza imbere synthesis ya kolagen, kunoza imikorere ihuriweho, no kuzamura imibereho yabasaza.
Ubuzima bwuruhu: Ubushakashatsi bwerekana ko Cartalax ishobora kongera ubworoherane nuburyo bwuruhu biteza imbere synthesis ya kolagen cyangwa kunoza imikorere ya selile yuruhu. Ibi bituma Cartalax ifite agaciro gakoreshwa mubijyanye no kwita ku ruhu no kurwanya gusaza, cyane cyane mu kunoza imiterere yuruhu no kugabanya iminkanyari [2].
Kurinda impyiko: Ubushakashatsi bumwe bwerekanye ko Cartalax ishobora kugira ingaruka zo kurinda imikorere yimpyiko n'imiterere. Irashobora kurinda impyiko kwangirika bigira ingaruka kumikorere ya selile mumpyiko, bikagabanya gucana no guhagarika umutima. Izi ngaruka zo kurinda impyiko zituma Cartalax igira agaciro gakoreshwa mukuvura indwara zimpyiko nindwara zifitanye isano.
Kurwanya gusaza: Cartalax ifatwa nka peptide irwanya gusaza ishobora gutinda ibintu bimwe na bimwe byubusaza bigira ingaruka muburyo butandukanye bwa selile. Irashobora kuzamura ubuzima muri rusange hamwe nubuzima muguhindura ingirabuzimafatizo, kugabanya imihangayiko ya okiside hamwe nibisubizo bitera, no guteza imbere gusana no kuvugurura. Ibi birwanya gusaza bituma Cartalax igira agaciro gakoreshwa mugutinda indwara ziterwa no gusaza no kuzamura ubuzima muri rusange [2].
Uburyo bwibikorwa bya Cartalax biragoye, bigira ingaruka ebyiri muburyo bwa okiside. Irashobora gukorana nibintu bitandukanye no kwigana imikorere ya antioxydeant. Porogaramu zayo ni nyinshi, zerekana ubushobozi mubice byinshi nko kuvugurura ingirangingo, gukira siporo, kuvura indwara, nubuzima bwuruhu.
Ibyerekeye Umwanditsi
Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.
Umwanditsi w'ikinyamakuru
Storozhok NM ni umushakashatsi ufitanye isano na kaminuza y’ubuvuzi ya Leta ya Tyumen, NN Semenov Federal Research Center for Physical Chemical mu Ishuri Rikuru ry’Ubumenyi ry’Uburusiya, na TYUMEN INST MED. Ibikorwa bye birimo ubushakashatsi butandukanye muri Chimie, Biochemie & Molecular Biology, Biophysics, Biologiya Cell, na Physics. Yatanze umusanzu ukomeye muriyi nzego binyuze mubikorwa bye byamasomo nubushakashatsi. Storozhok NM yanditse murutonde rwibisobanuro [1].
