Lyophilized, los yog khov-qhuav, peptides xav tau kev hloov kho qhov tseeb tsis txaus ntseeg kom ua haujlwm tau zoo. Kev tuav tsis raug feem ntau ua rau kev puas tsuaj sai, ua tiav kev ua tau zoo, thiab kev nqis peev hauv cov khoom muaj txiaj ntsig. Reconstitution yog cov txheej txheem tseem ceeb ntawm kev ntxiv cov kua diluent rau cov hmoov qhuav los tsim kom muaj kev ruaj khov, siv tau. Cov kauj ruam no txuas qhov sib txawv ntawm dormant chemical structures thiab active biological compounds. Yog tias koj maj cov txheej txheem no los yog siv cov kuab tshuaj tsis raug, koj yuav raug rhuav tshem cov ntawv cog lus zoo molecular mus tas li ua ntej lawv txawm mus txog theem kev sim. Hauv phau ntawv qhia dav dav no, koj yuav kawm txog cov txheej txheem tsim nyog kom muaj kev nyab xeeb thiab khaws cov khoom sib xyaw no kom ua rau lawv lub neej ntev. Peb npog txhua yam los ntawm kev xaiv diluent thiab kev npaj tsis muaj menyuam mus rau kev suav tshuaj kom raug. Tsis lees paub: Cov kev cai uas tau teev tseg hauv qab no yog nruj me ntsis rau kev kawm, kev tshawb fawb, lossis lub hom phiaj ntawm daim ntawv thov kev kuaj mob nkaus xwb.
Cov Lus Qhia
Sterility tsis tuaj yeem sib tham tau: Kev siv tshuaj kho mob qib sterile prep tiv thaiv kev kis kab mob thiab kev loj hlob ntawm cov kab mob.
Kev xaiv diluent dictates viability: Bacteriostatic dej yog tus qauv, tab sis cov peptide profile tshwj xeeb (hydrophilic vs. hydrophobic) yuav xav tau cov dej tsis huv, acetic acid, lossis DMSO.
Mechanical fragility: Peptides rhiab heev rau shear stress; tsis txhob co lub vial. Swirl maj mam khaws cia molecular bonds.
Txias-chain cia: Thaum rov tsim dua, peptides yuav tsum tau khaws cia hauv tub yees (feem ntau 36 ° F txog 46 ° F) thiab tiv thaiv los ntawm lub teeb kom muaj kev ncaj ncees.
Manufacturers feem ntau xa khoom peptides raws li lyophilized hmoov. Cov txheej txheem khov-ua kom qhuav no tshem tawm cov dej los ntawm qhov sib xyaw thaum khaws cia nws cov qauv amino acid complex. Nyob rau hauv ib lub xeev qhuav, cov tshuaj sib xyaw ua kom muaj kev ruaj ntseg mus ntev. Lawv tuaj yeem yooj yim muaj sia nyob qhov kub hloov pauv thaum lub sijhawm hla ntiaj teb. Yog tsis muaj dej, enzymatic degradation nres kiag li. Cov hmoov tseem nyob dormant thiab ruaj khov heev kom txog thaum koj qhia txog cov kua ua kua.
Kev ua tsis tiav cov hmoov no kom raug ua rau muaj kev phom sij loj heev. Kev hloov kho tsis raug sai sai denatures cov ntaub ntawv tsis yooj yim tuav cov amino acids ua ke. Thaum cov ntawv cog lus no tawg, lub compound poob nws cov khoom noj muaj txiaj ntsig zoo. Tsis tas li ntawd, cov txheej txheem tsis zoo tsis zoo qhia txog cov kab mob sib kis. Cov kab mob loj hlob hauv cov dej ib puag ncig, tshwj xeeb tshaj yog cov kev daws teeb meem muaj protein ntau. Kev kis kab mob tsis yog tsuas yog rhuav tshem lub txee lub neej tab sis kuj ua rau muaj kev nyab xeeb loj heev rau txhua daim ntawv thov tom ntej. Kev tuav tswj kom zoo txo cov kev piam sij no thiab tiv thaiv koj cov peev txheej.
Peb ntsuas qhov kev tsim kho kom zoo siv peb qhov kev sib txawv. Ua ntej, qhov kev daws teeb meem kawg yuav tsum tau meej meej. Cloudiness qhia hais tias undissolved hais los yog ib tug tshuaj tiv thaiv. Qhov thib ob, koj yuav tsum tswj hwm ib puag ncig kom tsis muaj menyuam txij thaum pib mus rau qhov kawg. Thaum kawg, cov txheej txheem yuav tsum ua kom muaj qhov tseeb concentration piv. Precise dosing yog tsis yooj yim sua yog tias koj miscalculate lub ntim hnyav.
Xaiv qhov tseeb diluent yog qhov kev txiav txim siab tseem ceeb. Cov kuab tshuaj uas koj xaiv txiav txim siab seb cov hmoov yaj puas zoo thiab ntev npaum li cas cov tshuaj tseem siv tau. Bacteriostatic dej, feem ntau hu ua BAC dej, ua haujlwm raws li tus qauv kev lag luam. Nws muaj 0.9% benzyl cawv. Qhov tshwj xeeb cawv concentration nquag tiv thaiv kab mob loj hlob hauv lub vial. Los ntawm inhibiting cov kab mob, BAC dej txuas ntxiv lub txee lub neej ntawm cov kev daws teeb meem mus txog 28 hnub. Cov kws tshawb fawb nyiam nws rau yuav luag txhua daim ntawv thov kev sim dav dav vim nws txoj kev khaws cia zoo.
Sterile Water for Injection (SWFI) muaj lwm txoj hauv kev, tab sis nws muaj cov kev txwv tseem ceeb. Tsis zoo li BAC dej, SWFI muaj kiag li tsis muaj preservatives. Nws muaj cov dej ntshiab, tsis muaj menyuam. Vim tias nws tsis muaj benzyl cawv, koj yuav tsum siv nws ua ib qho tshuaj diluent. Yog tias koj khaws lub vial reconstituted hauv SWFI, koj ntsib ib qho kev pheej hmoo siab ntawm cov kab mob sib kis hauv 24 teev. Koj tsuas yog yuav tsum siv SWFI rau tam sim, ib zaug thov.
Cov qauv siv ntau heev lossis hydrophobic xav tau cov kuab tshuaj tshwj xeeb. Hydrophobic txhais tau hais tias cov tshuaj siv tshuaj nquag tawm tsam dej. Nyob rau hauv cov ntaub ntawv no, txheem dej yuav tsis yaj lub hmoov ncuav mog qab zib. Cov kws tshaj lij hauv chav ntsuas ntsuas qhov tshwj xeeb amino acid ib ntus txhawm rau txiav txim siab txog qhov zoo tshaj plaws. Lawv feem ntau siv Dimethyl Sulfoxide (DMSO) los yog diluted acetic acid heev. Cov kuab tshuaj tshwj xeeb no rhuav tshem cov tawv nqaij molecular teeb meem. Ib zaug yaj hauv ib qho me me ntawm DMSO, koj tuaj yeem dilute qhov sib tov ntxiv siv cov dej BAC.
Hom Tshuaj Diluent |
Preservative suav nrog |
Thawj Siv Case |
Kwv yees lub neej txee |
|---|---|---|---|
Dej Bacteriostatic Dej (BAC) |
0.9% Benzyl cawv |
Cov qauv kev lag luam dav dav |
Txog li 28 hnub |
Sterile Dej (SWFI) |
Tsis muaj |
Ib zaug siv tam sim ntawd |
Hauv qab 24 teev |
DMSO / Acetic Acid |
Nws txawv ntawm qhov sib tov |
Hydrophobic lossis complex chains |
Nyob ntawm theem nrab dilution |
Koj tsis tuaj yeem cuam tshuam txog cov khoom siv zoo. Kev sib sau cov cuab yeej raug ua kom muaj kev sib tw, tsis muaj menyuam. Ib txwm npaj koj qhov chaw ua haujlwm ua ntej tuav cov vials. Ib qhov chaw tsis sib haum xeeb ua rau muaj kev pheej hmoo ntawm kev kis kab mob. Xyuas kom koj muaj cov khoom siv tseem ceeb hauv qab no npaj rau ntawm qhov chaw huv:
Ib lub hnab ntim lyophilized peptide vial
Koj xaiv diluent (feem ntau BAC dej)
Kev kho mob qib 70% isopropyl cawv swabs
Ib lub sterile Luer-lock mixing syringe (feem ntau yog 3ml txog 5ml muaj peev xwm)
Txheem ntsuas syringes (1ml / 100-unit)
Pib koj txoj cai huv huv los ntawm kev ntxuav koj txhais tes kom huv si. Muab cov hnab looj tes kho mob qib. So koj qhov chaw ua haujlwm tag nrho siv cov tshuaj tua kab mob. Tom ntej no, rub lub kaus mom yas tawm ob lub diluent vial thiab hmoov vial. Siv ib lub pas dej tshiab tshiab thiab siv zog so ob qho tib si roj hmab stoppers. Cia cov cawv kom cua qhuav tag. Tsis txhob tshuab rau cov nres, vim tib neeg ua pa muaj cov kab mob suav tsis txheeb.
Precision thaum lub sij hawm hloov theem tiv thaiv molecular puas. Koj yuav tsum tswj cov dej ntws zoo meticulously. Ua raws li cov kauj ruam hauv qab no kom paub meej tias kev nyab xeeb:
Kos cov huab cua ambient rau hauv koj lub koob txhaj tshuaj sib npaug ntawm qhov ntim ntawm diluent koj npaj yuav rho tawm.
Pierce lub diluent vial stopper thiab thawb cua hauv. Qhov no sib npaug ntawm lub siab thiab ua rau kev kos cov kua yooj yim dua.
Hloov lub vial thiab kos cov lej suav ntawm cov kua rau hauv koj lub koob txhaj tshuaj. Tshem cov koob.
Ua tib zoo tho cov roj hmab stopper ntawm cov hmoov vial.
Hais txog lub tshuab nqus tsev tam sim ntawd. Ntau lub lyophilized vials muaj lub tshuab nqus tsev muaj zog. Cia lub tshuab nqus tsev no rub cov kua los ntawm koj lub syringe lawm.
Direct cov kua dej tawm tsam lub iav phab ntsa ntawm lub vial. Tsis txhob tua cov kua ncaj qha rau ntawm lub ncuav mog qab zib uas tsis muaj zog.
Kev taw qhia cov kwj deg hauv phab ntsa iav yog ib txoj cai tseem ceeb rau kev siv. Nws tiv thaiv cov hmoov los ntawm kev raug mob hnyav ntawm lub cev. Cov kuab tshuaj yuav tso dej rau hauv qab thiab maj mam nqus cov khoom xyaw qhuav.
Tuav qhov kev pheej hmoo siab tshaj plaws thaum lub sijhawm tawg. Koj yuav tsum qhia meej kom tsis txhob co lub vial. Shaking qhia kev nyuaj siab shear. Nws yuam cua npuas rau hauv qhov sib tov thiab tuaj yeem rhuav tshem cov amino acid chains sib nrug. Hloov chaw, tuav lub vial maj mam. Yob nws maj mam nruab nrab ntawm koj ob txhais tes. Xwb, siv ib qho qeeb heev, nruam swirling motion.
Tom qab ob peb feeb ntawm maj mam swirling, ua ib qho kev kuaj pom. Tuav lub vial mus txog qhov chaw teeb pom kev zoo. Qhov kawg daws yuav tsum yog 100% pob tshab. Koj yuav tsum pom tsis muaj cov teeb meem ntab ntab, huab cua, lossis gelatinous clumps. Yog tias cov kua dej tseem meej meej, cov txheej txheem rov tsim dua tau ua tiav. Tam sim no koj tuaj yeem txav mus rau kev suav koj cov kev ntsuas tshwj xeeb.
Mastering concentration xam xam tshem tawm txaus ntshai dosing yuam kev. Koj yuav tsum nkag siab raws nraim li cas cov tshuaj nquag muaj nyob hauv txhua milliliter ntawm koj cov tshuaj tov tshiab. Peb txiav txim siab qhov no siv qhov ntsuas ncaj nraim. Cov qauv tseem ceeb yuav tsum faib cov hmoov tag nrho los ntawm tag nrho cov kuab tshuaj ntim.
Cov qauv tseem ceeb: Tag nrho mg ntawm Peptide / Tag Nrho ml ntawm Diluent = Concentration ib ml.
Ua ntej siv cov mis, koj xav tau ib lub hauv paus hloov dua siab tshiab. Vials teev lawv cov ntsiab lus hauv milligrams (mg). Cov koob txhaj tshuaj feem ntau ntsuas cov koob tshuaj hauv micrograms (mcg). Koj yuav tsum hloov cov units no kom raug. Tus qauv universal dictates tias 1 milligram sib npaug 1000 micrograms. Ua qhov kev hloov dua siab tshiab no rau lub cim xeeb tiv thaiv qhov kev ntsuas kev ntsuas ntau tshaj plaws.
Cia peb tshawb txog qhov ua piv txwv qhov xwm txheej. Xav txog tias koj muaj ib lub vial uas muaj 5mg ntawm lyophilized hmoov. Koj txiav txim siab sib tov nws siv 2ml ntawm BAC dej. Siv cov qauv tseem ceeb: 5mg muab faib los ntawm 2ml sib npaug 2.5mg ib 1ml. Tom qab ntawd peb hloov cov txiaj ntsig mus rau micrograms. Muab 2.5mg los ntawm 1000. Tam sim no koj paub tias koj cov tshuaj muaj 2500mcg ntawm cov tshuaj nquag hauv txhua milliliter.
Tom ntej no, koj yuav tsum tau txhais qhov concentration no rau cov cim zuam syringe. Tus qauv 1ml insulin-style syringe muaj 100 units (tick marks). Txhua chav nruab nrab yog 0.01ml ntawm cov kua. Yog tias 1ml tuav 2500mcg, peb faib 2500 los ntawm 100 units. Qhov no txhais tau hais tias txhua tus neeg zuam cim ntawm lub koob txhaj tshuaj muaj 25mcg ntawm cov tshuaj. Yog tias koj tus txheej txheem xav tau daim ntawv thov 250mcg, koj yuav kos cov kua mus txog 10-unit mark.
Syringe Units (1ml / 100U) |
Cov khoom ntim (ml) |
Dose (5mg sib xyaw hauv 2ml) |
Dose (10mg sib xyaw hauv 2ml) |
|---|---|---|---|
10 units |
0.1ml ib |
250 mcg |
500 mcg |
20 units |
0.2 ml ib |
500 mcg |
1000 mcg |
50 units |
0.5 ml yog ' |
12 50 mcg |
2500 mcg |
Phau ntawv lej yoojyim rau tib neeg yuam kev. Peb pom zoo kom siv lub laij lej online uas tau lees paub tau tsim tshwj xeeb rau cov khoom sib txuas no. Nkag mus rau koj lub vial loj, ntim ntim, thiab qhov xav tau microgram tus nqi. Lub cuab yeej yuav tso tawm qhov ntsuas ntsuas qhov xav tau. Siv lub laij lej digital los kuaj xyuas koj phau ntawv qhia lej txhawb txoj hauv kev tsis nyab xeeb rau kev tshawb fawb cov txheej txheem.
Environmental factor dictate txee lub neej. Koj yuav tsum ua raws li cov txheej txheem ntsuas kub nruj los tiv thaiv koj cov khoom muag. Pre-reconstitution cia nyob ntawm koj ncua sij hawm. Rau kev khaws cia ntev ntev li ob peb lub hlis, khaws cov lyophilized vials nyob rau hauv lub freezer tob ntawm -20 ° C. Rau lub sijhawm luv luv cia nyob rau hauv ob peb lub lis piam, lub tub yees txias ntawm 2 ° C txog 8 ° C ua haujlwm zoo kawg nkaus. Txawm li cas los xij, cov cai tom qab hloov kho tshiab hloov tag nrho. Thaum cov kua nkag mus rau hauv lub vial, cov tshuaj yuav tsum nyob twj ywm nruj me ntsis rau hauv tub yees ntawm 2 ° C mus rau 8 ° C. Tsis txhob khov cov kua dej.
Lub teeb thiab oxidation ua rau muaj kev pheej hmoo loj heev rau molecular kev ncaj ncees. Lub teeb ultraviolet rhuav tshem cov tshuaj lom neeg sib txuas sai sai los ntawm kev yees duab-oxidation. Koj yuav tsum khaws cov vials nyob rau hauv lub teeb-pov thawj ntim. Yog tias koj tsis muaj cov thawv tshwj xeeb, khaws cia rau hauv qhov chaw tsaus nti, zoo li lub kaum sab xis ntawm lub tub rau khoom tub yees. Txwv tsis pub lawv raug rau ambient chav teeb kom ntau li ntau tau thaum lub sij hawm hloov mus.
Kev nkag siab txog qhov tseeb ntawm txee lub neej tiv thaiv kev siv tsis raug ntawm cov khoom siv degraded. Cov ntaub ntawv pov thawj kho mob muab lub hauv paus meej meej rau kev tas sij hawm. Feem ntau peptides reconstituted siv BAC dej tuav lub ncov stability rau kwv yees li 3 mus rau 4 lub lis piam. Tom qab hnub 28, benzyl cawv poob nws qhov kev tiv thaiv muaj zog. Lub compound nws tus kheej pib tawg, poob sai sai. Nco ntsoov sau koj lub vials nrog rau hnub kawg ntawm kev kho dua tshiab. Koj yuav tsum muab pov tseg ib qho kev daws teeb meem uas qhia tau tias muaj huab cua, tsis muaj xim, lossis cov khoom ntab tam sim ntawd, tsis hais hnub twg.
Kev txhim kho kev ua tiav vam meej yog nyob ntawm peb lub hauv paus ncej: kev ua kom tsis muaj menyuam, kev suav lej lej, thiab kev tuav tes tshwj xeeb. Ua tsis tiav nyob rau hauv ib qho ntawm cov chaw no cuam tshuam cov txheej txheem kev ncaj ncees ntawm lub compound. Los ntawm kev nkag siab txog lub tshuab nqus tsev thiab saib xyuas qhov tsis muaj zog ntawm cov hmoov lyophilized, koj ua kom muaj kev nyab xeeb thiab ua tau zoo. Kev xaiv Diluent hnyav cuam tshuam rau lub neej ntev, ua BAC dej tus qauv tsis sib haum xeeb rau kev siv lub sijhawm ntev.
Koj cov kauj ruam tom ntej tam sim no suav nrog kev tshuaj xyuas koj cov khoom siv hauv chav kuaj tam sim no. Muab pov tseg ib qho uas tas sij hawm diluents lossis questionable vials. Qhov zoo tshaj plaws, tus neeg thib peb tau kuaj cov dej bacteriostatic los ntawm cov neeg muag khoom muaj npe nrov. Thaum kawg, siv cov cuab yeej ntsuas qhov tseeb thiab lub laij lej digital ua ntej pib nrog cov txheej txheem sib xyaw tshiab. Kev npaj kom zoo guarantees txhim khu kev qha, rov tsim tau cov txiaj ntsig txhua txhua lub sijhawm.
A: Cloudiness feem ntau qhia tias tsis tiav kev tawg, tsis raug xaiv diluent, lossis tsis zoo. Cov qauv hydrophobic feem ntau tsis lees txais cov qauv dej thiab tig cov kua mis. Xwb, qhov kub thiab txias hloov thaum lub sij hawm xa khoom tuaj yeem ua rau cov hmoov puas. Yog tias maj mam swirling tsis tshem cov kua tom qab peb caug feeb, muab pov tseg lub vial tag nrho. Cov kev daws teeb meem huab cua tsis tuaj yeem pom thiab tsis zoo rau kev thov.
A: Tsis yog. Koj yuav tsum nruj me ntsis zam cov kua dej khov. Lub voj voog khov-thaw ua rau cov dej molecules nthuav dav thiab cog lus aggressively. Qhov kev nthuav dav ntawm lub cev no tsim cov dej khov nab kuab microscopic. Cov crystals no shear cov ilv amino acid bonds, ua rau muaj kev puas tsuaj loj heev. Nco ntsoov khaws cov vials reconstituted tub yees ntawm 36 ° F thiab 46 ° F.
A: Kev co qhia txog kev ua phem ntawm kev ntxhov siab thiab ua rau muaj npuas sai hauv lub vial. Qhov kev raug mob txhua yam tuaj yeem ua rau cov khoom sib txuas ntawm cov protein, ua rau tsis muaj txiaj ntsig mus tas li. Yog tias koj ua yuam kev, muab lub vial tso rau hauv lub tub yees tam sim ntawd. Cia nws nyob twj ywm undisturbed rau 24 teev. Nco ntsoov qhov ua tau zoo poob thaum koj qhov kev ntsuam xyuas.
A: Raws li cov txheej txheem kho mob, cov dej bacteriostatic tseem muaj kev nyab xeeb rau 28 hnub tom qab puncture. 0.9% benzyl cawv degrades lub sij hawm thaum raug rau huab cua thiab ambient contaminants. Tom qab plaub lub lis piam, nws tsis tuaj yeem lav qhov kev tiv thaiv kab mob loj hlob ntxiv lawm. Ib txwm muab pov tseg BAC qhib dej rau hnub 29.
