Lyophilized, cyangwa gukonjesha-yumye, peptide isaba kwiyubaka bidasanzwe kugirango ikore neza. Gukemura nabi akenshi biganisha ku kwangirika byihuse, gutakaza burundu imikorere, no gushora ubusa mubikoresho byagaciro. Kwiyubaka ni inzira yingenzi yo kongeramo amazi ya poro yumye kugirango habeho igisubizo gihamye, cyakoreshwa. Iyi ntambwe ikuraho itandukaniro riri hagati yimiterere yimiti idasinziriye hamwe nibinyabuzima bikora. Niba wihutisha protocole cyangwa ugakoresha igisubizo kitari cyo, ushobora guhungabanya imiyoboro ya molekile yoroheje burundu mbere yuko igera mukigeragezo. Muri ubu buyobozi bwuzuye, uziga protocole nyayo isanzwe yo kuvanga neza no kubika ibyo bikoresho kugirango ubeho igihe kirekire. Dutwikiriye ibintu byose kuva guhitamo neza no gutegura sterile kugeza kubara neza. Inshingano: Porotokole yavuzwe hepfo ni iy'uburezi, ubushakashatsi, cyangwa intego za laboratoire ziteganijwe gusa.
Imbonerahamwe
Kutabyara ntibishobora kuganirwaho: Gushyira mubikorwa ivuriro-sterile itegura birinda kwanduza no gukura kwa bagiteri.
Guhitamo umwete bisaba kubaho neza: Amazi ya bacteriostatike niyo asanzwe, ariko imyirondoro yihariye ya peptide (hydrophilique na hydrophobique) irashobora gusaba amazi meza, aside acike, cyangwa DMSO.
Kugabanuka kwa mashini: Peptide yunvikana no guhagarika umutima; ntuzigere uhungabanya inkono. Kuzenguruka witonze kugirango ubungabunge ingirabuzimafatizo.
Ububiko bukonje bukonje: Bumaze gusubirwamo, peptide igomba guhora ikonjeshwa (mubisanzwe 36 ° F kugeza 46 ° F) kandi ikarindwa urumuri kugirango igumane ubusugire bwimiterere.
Ababikora bohereza byinshi peptide nkifu ya lyofilize. Ubu buryo bwo gukonjesha bukuraho amazi mu kigo mugihe cyo kubungabunga imiterere ya aside amine igoye. Mugihe cyumye, imiti yimiti igera kumurongo muremure. Birashobora kurokoka byoroshye ihindagurika ryubushyuhe mugihe cyo gutambuka kwisi. Nta mazi, kwangirika kwa enzymatique guhagarara rwose. Ifu ikomeza gusinzira kandi ihagaze neza kugeza utangije umusemburo wamazi.
Kunanirwa gusubiramo iyi poro neza bitwara ingaruka zikomeye za chimique. Kwiyubaka bitari byo byerekana byihuse imigozi yoroheje ifata aside amine hamwe. Iyo ubwo bucuti bwacitse, urugimbu rutakaza ubuzima bwibinyabuzima rwose. Byongeye kandi, tekinike mbi ya sterile itangiza kwanduza bagiteri. Indwara ya bagiteri itera imbere mubidukikije, cyane cyane ibisubizo bikungahaye kuri poroteyine. Kwanduza ntabwo byangiza ubuzima bwubuzima gusa ahubwo binateza umutekano muke kubisabwa byose. Gukemura neza bigabanya izo ngaruka kandi bikarinda ishoramari ryawe.
Dupima kwiyubaka neza dukoresheje ibintu bitatu bitandukanye. Icya mbere, igisubizo cyanyuma kigomba kuba gisobanutse rwose. Igicu cyerekana ibice bidakemutse cyangwa reaction ya chimique. Icya kabiri, ugomba gukomeza kubungabunga ibidukikije kuva utangiye kugeza urangiye. Hanyuma, inzira igomba gutanga igipimo nyacyo cyo kwibanda. Gukoresha neza ntibishoboka niba ubara nabi ingano ya solvent.
Guhitamo neza imvugo nicyemezo gikomeye. Umuti wahisemo ugena uburyo ifu ishonga neza nigihe igisubizo gikomeza kuba cyiza. Amazi ya bacteriostatike, azwi kwizina rya BAC, akora nkurwego rwinganda. Harimo 0,9% inzoga ya benzyl. Iyi alcool yihariye yibuza cyane gukura kwa bagiteri imbere muri vial. Muguhagarika bagiteri, amazi ya BAC yongerera igihe cyubuzima bwibisubizo byubatswe kugeza kumunsi 28. Abashakashatsi bahitamo hafi ya laboratoire rusange muri rusange kubera imiterere yizewe yo kubungabunga.
Amazi ya Sterile yo gutera inshinge (SWFI) atanga ubundi buryo, ariko butwara imbogamizi zikomeye. Bitandukanye n'amazi ya BAC, SWFI irimo rwose kubika ibintu. Igizwe n'amazi meza, meza. Kuberako ibuze inzoga ya benzyl, ugomba kuyikoresha nkumuti umwe gusa. Niba ubitse vial yongeye gushyirwaho muri SWFI, uhura ningaruka zidasanzwe zo kwandura bagiteri mugihe cyamasaha 24. Ugomba gukoresha SWFI gusa kubintu byihuse, inshuro imwe.
Byinshi cyane cyangwa hydrophobique formulaire ikenera umwihariko. Hydrophobique bisobanura urunigi rwa chimique rwirukana amazi. Muri ibi bihe, amazi asanzwe ntashobora gushonga ifu yifu. Abatekinisiye ba laboratoire basuzuma aminide acide yihariye kugirango bamenye inzira nziza. Bakunze gukoresha Dimethyl Sulfoxide (DMSO) cyangwa acide acike cyane. Iyi mikorere yihariye isenya inzitizi zinangiye. Iyo bimaze gushonga mukantu gato ka DMSO, mubisanzwe urashobora kuvanga imvange ukoresheje amazi asanzwe ya BAC.
Ubwoko bw'ingutu |
Kubungabunga |
Ikoreshwa ryibanze |
Bigereranijwe Ubuzima bwa Shelf |
|---|---|---|---|
Amazi ya bacteriostatike (BAC) |
0,9% Inzoga ya Benzyl |
Inganda rusange |
Kugera ku minsi 28 |
Amazi meza (SWFI) |
Nta na kimwe |
Koresha inshuro imwe |
Munsi yamasaha 24 |
DMSO / Acide Acike |
Biratandukanye bivanze |
Hydrophobic cyangwa iminyururu igoye |
Biterwa na dilution ya kabiri |
Ntushobora guteshuka ku bwiza bwibikoresho. Gukusanya ibikoresho byiza bituma inzira igenda neza. Buri gihe utegure aho ukorera mbere yo gukora vial. Umwanya udahwitse wongera ibyago byo kwanduza. Menya neza ko ufite ibikoresho bikurikira bikurikira byiteguye hejuru yisuku:
Umuyoboro umwe wafunzwe na peptide
Ururimi wahisemo (mubisanzwe amazi ya BAC)
Ubuvuzi-70% isopropyl inzoga swabs
Sterile Luer-lock ivanga syringe (mubisanzwe 3ml kugeza 5ml)
Siringi yo gupima bisanzwe (1ml / 100-unit)
Tangira protocole yawe yisuku ukaraba intoki neza. Shira uturindantoki two mu rwego rwa clinique. Ihanagura ubuso bwawe bwose bukora ukoresheje disinfectant. Ubukurikira, shyira hejuru ya capitike ya pulasitike hejuru yikibindi cyoroshye. Fata inzoga nshya hanyuma uhanagure cyane ibyuma byombi. Emerera inzoga guhumeka neza. Ntugatere hejuru ahagarara, kuko umwuka wabantu utwara bagiteri zitabarika.
Icyitonderwa mugihe cyo kwimura birinda kwangirika kwa molekile. Ugomba kugenzura neza amazi. Kurikiza izi ntambwe zikurikiranye kugirango umenye neza umutekano:
Shushanya umwuka wibidukikije muri mixe yawe ivanze ihwanye nubunini bwa diluent uteganya gukuramo.
Gutobora vial ihagarara hanyuma usunike umwuka imbere. Ibi binganya igitutu kandi bituma gushushanya byoroshye.
Hindura vial hanyuma ushushanye ingano yuzuye yabazwe mumazi muri syringe. Kuraho urushinge.
Witonze utobore reberi ihagarika ifu yifu.
Kemura ikibazo cya vacuum ako kanya. Ibikoresho byinshi bya lyofilize birimo icyuho gikomeye imbere. Reka iyi vacuum ikure amazi muri syringe bisanzwe.
Kuyobora umugezi wamazi kurukuta rwimbere rwikirahure. Ntuzigere urasa amazi kuri cake yoroheje.
Kuyobora umugezi munsi yurukuta rwikirahure ni itegeko ryingenzi ryo gushyira mubikorwa. Irinda ifu guhura nihungabana ryumubiri. Umuti uzahuza hepfo hanyuma winjize witonze ibice byumye.
Gukemura ibibazo biri hejuru mugihe cyo gusesa. Ugomba kwirinda byimazeyo kunyeganyeza vial. Kunyeganyeza bizana impagarara zikomeye. Ihatira umwuka mubi muvanga kandi irashobora gutandukanya iminyururu ya aside amine. Ahubwo, fata inkono witonze. Kuzunguruka buhoro hagati y'intoki zawe. Ubundi, koresha gahoro gahoro, uhoraho uzunguruka.
Nyuma yiminota mike yo kuzunguruka witonze, kora igenzura. Fata inkono kugeza isoko yumucyo. Igisubizo cyanyuma kigomba kuba mucyo 100%. Ntugomba kubona ntakintu kireremba hejuru, ibicu, cyangwa gelatinous clumps. Niba amazi akomeje kugaragara neza, inzira yo kwiyubaka yagenze neza. Urashobora noneho kwimuka kubara ibipimo byihariye.
Kumenya kubara kwibanda gukuraho amakosa yo kunywa. Ugomba gusobanukirwa neza nuburyo ibintu bifatika bibaho muri mililitiro yumuti wawe mushya uvanze. Turabigena dukoresheje igipimo cyo gusuzuma neza. Inzira yibanze isaba kugabanya ifu yuzuye hamwe nubunini bwuzuye.
Inzira yibanze: mg yose ya Peptide / ml yose ya Diluent = Kwishyira hamwe kuri ml.
Mbere yo gukoresha formulaire, ukeneye ihinduka rikomeye. Viali yerekana ibiri muri miligarama (mg). Siringes isanzwe ipima dosiye muri microgrammes (mcg). Ugomba guhindura ibice neza. Igipimo rusange gitegeka ko miligarama 1 ihwanye na microgramo 1000. Gukora iyi mpinduramatwara birinda amakosa yo gupima laboratoire.
Reka dusuzume urugero rufatika. Tekereza ufite vial irimo 5mg yifu ya lyofilize. Uhisemo kubivanga ukoresheje 2ml y'amazi ya BAC. Gukoresha formulaire yibanze: 5mg igabanijwe na 2ml ihwanye na 2,5mg kuri 1ml. Hanyuma duhindura ibisubizo kuri microgrammes. Kugwiza 2.5mg kuri 1000. Ubu uzi ko igisubizo cyawe kirimo 2500mcg yingirakamaro ikora muri mililitiro imwe.
Ibikurikira, ugomba guhindura iyi concentration kubimenyetso bya singe. Siringi isanzwe ya 1ml ya insuline irimo ibice 100 (amatiku). Buri gice kigereranya 0.01ml y'amazi. Niba 1ml ifite 2500mcg, tugabanya 2500 kubice 100. Ibi bivuze ko buri tike yikimenyetso kuri syringe irimo 25mcg yikigo. Niba protocole yawe isaba 250mcg ya progaramu, washobora gushushanya amazi kugeza kumurongo 10.
Ibice bya Syringe (1ml / 100U) |
Umubumbe wakuweho (ml) |
Dose (5mg ivanze muri 2ml) |
Dose (10mg ivanze muri 2ml) |
|---|---|---|---|
Ibice 10 |
0.1 ml |
250 mcg |
500 mcg |
Ibice 20 |
0.2 ml |
500 mcg |
1000 mcg |
50 Units |
0.5 ml |
1250 mcg |
2500 mcg |
Imibare yintoki isiga umwanya wikosa ryabantu. Turasaba cyane ko dukoresha ibarwa yo kuri interineti igenzurwa byabugenewe. Shyiramo ubunini bwa vial, ingano ya diluent, hamwe na microgramu wifuza. Igikoresho kizasohoka umubare wuzuye usabwa. Gukoresha ibara rya digitale kugirango ugenzure kabiri imibare y'intoki ishimangira uburyo bwananiwe umutekano kuri protocole yubushakashatsi.
Ibidukikije bigena ubuzima bubi. Ugomba gushyira mubikorwa protocole yubushyuhe kugirango urinde ububiko bwawe. Kubika mbere yo kwiyubaka biterwa nigihe cyawe. Kubika igihe kirekire mumezi menshi, shyira viali ya lisofili muri firigo yimbitse kuri -20 ° C. Kubika igihe gito mugihe cyibyumweru bike, firigo isanzwe kuri 2 ° C kugeza 8 ° C ikora neza. Ariko, amategeko nyuma yo kwiyubaka arahinduka rwose. Amazi amaze kwinjira muri vial, igisubizo kigomba kuguma gikonjeshwa cyane kuri 2 ° C kugeza 8 ° C. Ntugahagarike ibisubizo byamazi.
Umucyo na okiside bitera ingaruka zikomeye kubunyangamugayo. Umucyo Ultraviolet usenya imiti igoye byihuse binyuze mumafoto-okiside. Ugomba buri gihe kubika ibibindi mubikoresho bitarimo urumuri. Niba ubuze ibikoresho byabugenewe, ubibike ahantu hijimye rwose, nkimfuruka yinyuma ya firigo. Gabanya imurikagurisha ryumucyo wicyumba gishoboka mugihe cyo kwimura.
Gusobanukirwa nubuzima bwubuzima burinda ikoreshwa ryimpanuka ibintu byangiritse. Ibimenyetso byamavuriro bitanga urufatiro rusobanutse rwo kurangira. Benshi peptide yongeye gukoreshwa hifashishijwe amazi ya BAC igumya guhagarara neza mugihe cibyumweru 3 kugeza 4. Nyuma yumunsi wa 28, inzoga ya benzyl itakaza imbaraga zo kurinda. Urusange ubwacyo rutangira gusenyuka, gutakaza imikorere byihuse. Buri gihe andika ibikono byawe nitariki nyayo yo kwiyubaka. Ugomba guta ibisubizo byose byerekana ibicu, ibara, cyangwa ibice bireremba ako kanya, utitaye kumunsi.
Kwiyubaka bigenda neza bishingiye gusa ku nkingi eshatu zifatizo: sterité absolute, kubara neza imibare, no gufata neza bidasanzwe. Kunanirwa muri kimwe muribi bibangamira uburinganire bwimiterere yikigo. Mugusobanukirwa n'ingaruka za vacuum no kubaha intege za porojeri ya lyofilize, uragabanya umutekano ndetse ningirakamaro. Guhitamo gukorana umwete bigira uruhare runini kuramba, bigatuma amazi ya BAC atavuguruzwa mugukoresha laboratoire igihe kirekire.
Intambwe zawe zikurikira zirimo kugenzura ibikoresho byawe bya laboratoire. Hagarika ibyaribyo byose byarangiye cyangwa inzabya zikemangwa. Inkomoko yujuje ubuziranenge, igice cya gatatu cyapimwe amazi ya bacteriostatike kubacuruzi bazwi. Hanyuma, koresha ibikoresho byo gupima neza hamwe na calculatrice mbere yo gukomeza na protocole nshya ivanze. Gutegura neza byemeza ibisubizo byizewe, byororoka buri gihe.
Igisubizo: Igicu mubisanzwe byerekana guseswa kutuzuye, guhitamo nabi, cyangwa ubuziranenge bwangiritse. Hydrophobique yubatswe akenshi yanga amazi asanzwe kandi ihinduka amata. Ubundi, ihindagurika rikabije ryubushyuhe mugihe cyo kohereza rishobora kwangiza ifu. Niba kuzunguruka byoroheje bidasiba amazi nyuma yiminota mirongo itatu, fata vial burundu. Ibisubizo byigicu ntibiteganijwe kandi bifite umutekano kubisabwa.
Igisubizo: Oya. Ugomba kwirinda rwose gukonjesha ibisubizo byamazi. Inzira ya freze-thaw itera molekile zamazi kwaguka no kwandura bikabije. Uku kwaguka kumubiri kurema microscopique ice kristal. Izi kristu zogosha aminide acide ya aminike yoroheje, itera kwangirika kwimiterere. Buri gihe ujye ubika firigo zongeye gukonjeshwa hagati ya 36 ° F na 46 ° F.
Igisubizo: Kunyeganyeza bizana impagarara zogosha kandi bitera ifuro ryihuse imbere muri vial. Ihahamuka ryimashini rishobora gutandukanya poroteyine, biganisha ku gutakaza burundu imikorere. Niba uhungabanije kubwimpanuka, shyira vial muri firigo ako kanya. Reka bikemuke bidahungabanye mumasaha 24. Reba igihombo gishobora guterwa mugihe cyo gusuzuma.
Igisubizo: Ukurikije amabwiriza asanzwe yubuvuzi, amazi ya bacteriostatike akomeza kuba umutekano muminsi 28 nyuma yo gutoborwa. Inzoga ya 0.9% ya benzyl yangirika mugihe cyo guhura numwuka hamwe nibidukikije. Nyuma yibyumweru bine, ntishobora kongera kwemeza gukumira imikurire ya bagiteri. Buri gihe ujugunye amazi ya BAC kumunsi wa 29.
