Lyophilized, alo tsikpe me ƒu, . peptidewo hiã be woagbugbɔ wo aɖo wo nɔewo pɛpɛpɛ ale gbegbe be woate ŋu awɔ dɔ nyuie. Zi geɖe la, ne womewɔa nu nyuie o la, egblẽa nu kabakaba, eƒe dɔwɔwɔ meganɔa anyi kura o, eye wògblẽa gadede nu xɔasiwo me. Gbugbɔgaɖoanyi nye nu vevi si wowɔna tsɔ dea tsi si me wodea tsii ɖe atikekui ƒuƒu me be wòawɔ atike si li ke, si woate ŋu azã. Afɔɖeɖe sia xea mɔ na vovototo si le atike siwo le alɔ̃ dɔm kple nugbagbevi siwo le dɔ wɔm dome. Ne èwɔ kaba le ɖoɖo sia me alo zã atike si mesɔ o la, àde afɔku me be yeatsrɔ̃ molecule ƒe kadodo siwo le bɔbɔe la tegbee hafi woaɖo dodokpɔ ƒe akpaa gɔ̃ hã. Le mɔfiame blibo sia me la, àsrɔ̃ ɖoɖo siwo tututu wowɔ ɖe ɖoɖo nu hena atike siawo tsakatsaka kple wo dzadzraɖo dedie be woanɔ agbe didi wu. Míeƒoa nu tso nusianu ŋu tso diluent tiatia kple sterile dzadzraɖo dzi va ɖo agbɔsɔsɔme ƒe akɔntabubu si sɔ dzi. Adzɔgbeɖeɖe: Ðoɖo siwo woɖe fia le ete la nye hehenana, numekuku, alo nudokpɔƒe zazã si woɖo ɖi ɖeɖeko tɔ.
Emenyawo ƒe Tabla
Vidzidzi nye nusi ŋu womate ŋu awɔ ɖoɖo ɖo o: Dɔlékuiwutikewo zazã le atikewɔƒewo xea mɔ na ɖiƒoƒo kple dɔlékuiwo ƒe tsitsi.
Diluent tiatia bia be wòanɔ agbe: Tsi si me dɔlékuiwo tsia tre ɖoe nye nusi wozãna, gake peptide ƒe nɔnɔme tɔxɛwo (hydrophilic vs. hydrophobic) ate ŋu abia tsi si me dɔlékui mele o, acetic acid, alo DMSO.
Mɔ̃ɖaŋununya ƒe gbagbã: Peptidewo sea veve ɖe lãɖeɖe ƒe nuteɖeamedzi nu; mèʋuʋu nugoe la gbeɖe o. Tsɔe ƒo xlãe blewuu be molecule ƒe kadodowo nanɔ anyi.
Nudzraɖoƒe le kɔsɔkɔsɔ fafɛ me: Ne wogbugbɔ wɔ peptidewo ko la, ele be woatsɔ wo ade nufamɔ̃ me (zi geɖe la, dzoxɔxɔ si tso 36°F va ɖo 46°F) eye woakpɔ wo ta tso kekeli me be woƒe wɔwɔme nawɔ ɖeka.
Adzɔnuwo wɔlawo ɖoa meli akpa gãtɔ peptides abe atike si wotsɔa lyophilized wɔe ene. Nu sia si wotsɔna ƒua tsii le tsikpe me ɖea tsi ɖa le atike si wotsɔ wɔe me esime wòléa eƒe amino acid ƒe wɔwɔme si sesẽ la ta. Le nɔnɔme si me tsi mele o me la, atikeawo te ŋu nɔa te ɣeyiɣi didi. Woate ŋu anɔ agbe bɔbɔe le dzoxɔxɔ ƒe tɔtrɔ me le xexeame katã ƒe mɔzɔzɔ me. Tsi manɔmee la, enzyme ƒe gbegblẽ nu tsona keŋkeŋ. Aŋɔa nɔa alɔ̃ dɔm eye wòli ke ŋutɔ vaseɖe esime nàde tsi si wotsɔna tsia ami me.
Ne womete ŋu gbugbɔ atike sia wɔ nyuie o la, atikewo ƒe afɔku sesẽwo le eme. Gbugbɔgaɖoanyi si mesɔ o gblẽa kadodo siwo mebɔbɔ o siwo léa amino acidwo ɖe te la kabakaba. Ne kadodo siawo me gblẽ la, atike si wotsɔ wɔa atikekui la megate ŋu nɔa agbe le nugbagbewo me kura o. Gakpe ɖe eŋu la, dɔlékuiwutikewo ƒe mɔnu gbegblẽ hea dɔlékuiwo ƒe ɖiƒoƒo vɛ. Dɔlékuiwo tsina nyuie le tsi ƒe nɔnɔmewo me, vevietɔ le tsi siwo me protein sɔ gbɔ ɖo me. Menye ɖeko ɖiƒoƒo gblẽa nudzraɖoƒea ƒe agbenɔƒe ko o, ke ehea dedienɔnɔ ƒe afɔku gãwo hã vɛ na dɔ ɖesiaɖe si woawɔ emegbe. Ne èwɔ nu ɖe eŋu nyuie la, eɖea afɔku siawo dzi kpɔtɔna eye wòkpɔa wò gadede asi ta.
Míezãa dzidzenu vovovo etɔ̃ tsɔ dzidzea gbugbɔgaɖoanyi dzidzedzetɔe. Gbã la, ele be egbɔkpɔnu mamlɛtɔ me nakɔ keŋkeŋ. Alilikpo dodo fia be nu suesuesue siwo me mekɔ o alo atikewo ƒe dɔwɔwɔ. Evelia, ele be nàlé nɔnɔme si me dɔlékui mele o me ɖe asi tso gɔmedzedze vaseɖe nuwuwu. Mlɔeba la, ele be dɔdamɔnua nana woakpɔ agbɔsɔsɔme si sɔ pɛpɛpɛ. Agbɔsɔsɔme si sɔ pɛpɛpɛ manya wɔ ne èbu akɔnta le atike ƒe agbɔsɔsɔme ŋu vodadatɔe o.
Tiatia si sɔ si woatsɔ akɔ tsii nye nyametsotso vevi aɖe. Atike si nètia lae afia alesi gbegbe atikekui la alɔ̃ nyuie kple ɣeyiɣi didi si atike la anɔ anyi. Tsi si tsia dɔlékuiwo nu, si woyɔna zi geɖe be BAC tsi, nyea dɔwɔƒea ƒe dzidzenu. Benzyl aha sesẽ 0.9% le eme. Aha ƒe agbɔsɔsɔ sia koŋ xea mɔ na dɔlékuiwo ƒe tsitsi le goa me vevie. To mɔxexe ɖe dɔlékuiwo nu me la, BAC tsi dzia tsi siwo wogbugbɔ wɔ ƒe agbenɔƒe ɖe edzi va ɖoa ŋkeke 28. Numekulawo lɔ̃nɛ wu na nudokpɔƒe ƒe dɔwɔwɔwo katã kloe le eƒe edzadzraɖo ƒe nɔnɔme siwo ŋu kakaɖedzi le ta.
Tsi si me dɔlékui mele o si wodona ɖe lãme na ame (SWFI) na mɔnu bubu aɖe, gake seɖoƒe gã aɖewo le eŋu. To vovo na BAC tsi la, atike aɖeke kura mele SWFI me o. Tsi dzadzɛ si me dɔlékui mele o ye le eme. Esi benzyl aha mele eme o ta la, ele be nàzãe abe atike si wozãna zi ɖeka ene. Ne èdzra atikekui si wogbugbɔ ƒo ƒu ɖo ɖe SWFI me la, afɔku gã tɔxɛ aɖe le ŋuwò be dɔlékuiwo ate ŋu aƒo ɖi wò le gaƒoƒo 24 me. Ele be nàzã SWFI na dɔwɔwɔ enumake, zi ɖeka ko.
Mɔnu siwo sesẽ ŋutɔ alo esiwo me tsi mele o hiã atike tɔxɛwo. Hydrophobic fia be atikewo ƒe kɔsɔkɔsɔa nya tsi vevie. Le nɔnɔme siawo me la, tsi si wozãna ɖaa matsɔ abolo si wotsɔa aŋe wɔe la aƒu gbe o. Nudokpɔƒe ƒe mɔ̃ɖaŋudɔwɔlawo daa amino acid ƒe ɖoɖo tɔxɛa kpɔna be yewoanya mɔnu nyuitɔ kekeake. Zi geɖe la, wozãa Dimethyl Sulfoxide (DMSO) alo acetic acid si me wode tsii ŋutɔ. Atike tɔxɛ siawo gblẽa molecule ƒe mɔxenu sesẽwo. Ne ènya ƒoe ɖe DMSO sue aɖe me ko la, zi geɖe la, àte ŋu azã BAC tsi si wozãna ɖaa atsɔ aɖiɖi tsakatsaka la ɖe edzi.
Diluent Ƒomevi |
Nusiwo Wotsɔ dzraa Nu Tae Le Eme |
Zãzã Gbãtɔ ƒe Nya |
Agbenɔƒe si Wobu Akɔntabubu |
|---|---|---|---|
Tsi si Tsi Dɔlékuiwo Nu (BAC) . |
0.9% Benzyl Aha sesẽ |
Dɔwɔƒewo ƒe dzidzenu si wozãna le mɔ gbadza nu |
Vaseɖe ŋkeke 28 sɔŋ |
Tsi si me dɔlékui mele o (SWFI) . |
Ɖeke o |
Dɔdamɔnu siwo wozãna enumake siwo wozãna zi ɖeka |
Gaƒoƒo 24 teti |
DMSO / Aɖi si woyɔna be Acid |
Etoa vovo le nusiwo wotsaka nu |
Kɔsɔkɔsɔ siwo me tsi mele o alo esiwo me kɔ |
Enɔ te ɖe evelia ƒe tsinyenye dzi |
Màte ŋu aɖe asi le dɔwɔnuwo ƒe nyonyome ŋu o. Dɔwɔnu siwo sɔ nuƒoƒoƒu nana be dɔa yia edzi nyuie, si me dɔlékui mele o. Dzra wò dɔwɔƒe ɖo ɣesiaɣi hafi nàtsɔ nugoeawo awɔ dɔe. Teƒe si womewɔ ɖoɖo ɖe eŋu o naa ɖiƒoƒo ƒe afɔkua dzina ɖe edzi. Kpɔ egbɔ be nu vevi siwo gbɔna la le klalo le teƒe si le dzadzɛ:
Peptide-go ɖeka si wotsɔ lyophilized tu si wotre
Diluent si nètia (zi geɖe la, BAC tsi) .
Atikewɔwɔ ƒe dzeside 70% isopropyl aha sesẽwo
Luer-lock mixing syringe si me dɔlékui mele o (zi geɖe la, eƒe ŋutete nye 3ml va ɖo 5ml) .
Dzidzenu ƒe atikekui siwo wozãna ɖaa (1ml/100-unit) .
Dze wò dzadzɛnyenye ƒe ɖoɖoa gɔme to asikɔklɔ nyuie me. Do asigɛ siwo sɔ na atikewɔƒe. Zã dɔlékuiwutike tutu wò dɔwɔƒe bliboa ɖe anyi. Eyome, tsɔ aŋekotokuawo ƒu gbe le tsi si me wodea tsii ƒe nugoe kple atikekui la siaa ŋu. Kplɔ aha yeye aɖe eye nàtutu rababa-nulénu eveawo siaa sesĩe. Na aha la naƒu le ya me keŋkeŋ. Mègaku ya ɖe nutrenuwo dzi o, elabena amegbetɔ ƒe gbɔgbɔ tsɔa dɔlékui manyaxlẽwo.
Ne wowɔe pɛpɛpɛ le asitɔtrɔ ƒe akpaa me xea mɔ na molecule ƒe gbegblẽ. Ele be nàkpɔ tsi ƒe sisi dzi nyuie. Wɔ ɖe afɔɖeɖe siawo siwo kplɔ wo nɔewo ɖo dzi be nàkpɔ egbɔ be yewɔ nu ɖe eŋu dedie:
He ya si ƒo xlã wò la ɖe wò atike si wotsɔ tsakaa nu me si sɔ kple tsi si nèɖo be yeaɖe ƒe agbɔsɔsɔme.
To diluent vial stopper la me eye nàtu yaa ɖe eme. Esia nana nyaƒoɖeamenu sɔ eye wònana tsi la hehe nɔa bɔbɔe.
Trɔ atikekui la eye nàhe tsi agbɔsɔsɔme si tututu wobu akɔntae la ɖe wò atikekui me. Ðe aŋea ɖa.
To rababa-nutrenu si le powder-goa me la me nyuie.
Kpɔ vacuum ƒe ŋusẽkpɔɖeamedzia gbɔ enumake. Vacuum sesẽ aɖe le nugoe geɖe siwo wotsɔ lyophilized me. Na yaƒoƒo sia nahe tsi si le wò atikekui me le dzɔdzɔme nu.
He tsi si le sisim la ɖe goa ƒe ahuhɔ̃e gli ememetɔ ŋu. Mègada tsi si le eme la ɖe abolo si me wodea amii si mebɔbɔ o la dzi tẽ gbeɖe o.
Tɔsisia ƒe mɔfiafia to glass-gli la dzi nye se vevi aɖe si woatsɔ awɔ dɔe. Exea mɔ na atikekui la be wòagaxɔ ŋutilã me nuveviwɔame ŋutasesẽtɔe o. Atikea aƒo ƒu ɖe ete eye wòaxɔ atike ƒuƒu la blewuu.
Afɔku ƒe kɔkɔƒe gbɔ kpɔkpɔ le ʋuʋu ƒe akpaa me. Ele be nàƒo asa na nugoe la ʋuʋu tẽ. Ʋuʋudedi hea lãɖeɖe ƒe nuteɖeamedzi sesẽ vɛ. Ezia ya ƒe kuɖɔwo dzi be woage ɖe tsakatsaka la me eye ate ŋu avuvu amino acid kɔsɔkɔsɔawo le ŋutilã me. Ke boŋ lé nugoe la ɖe asi blewu. Miae blewu le asibidɛwo dome. Alo zã ʋuʋu blewu ŋutɔ, si yia edzi ɖaa.
Le aɖabaƒoƒo ʋɛ aɖewo ƒe ʋuʋu blewu megbe la, wɔ ŋkuléle ɖe eŋu. Lé nugoe la ɖe kekelitsoƒe aɖe gbɔ. Ele be egbɔkpɔnu mamlɛtɔ nanye esi me kɔ 100%. Mele be nàkpɔ nu suesuesue siwo le tsia dzi, alilikpo, alo gelatin ƒe ƒuƒoƒo aɖeke o. Ne tsi la gakpɔtɔ me kɔ keŋkeŋ la, ke nuwo gbugbɔgaɖɔɖo ƒe ɖoɖoa kpɔ dzidzedze. Àte ŋu ayi wò dzidzenu tɔxɛwo ƒe akɔntabubu dzi azɔ.
Ne wobi ɖe akɔntabubu siwo ku ɖe agbɔsɔsɔme si woatsɔ awɔ dɔe ŋu me ɖea vodada siwo me afɔku le le atikeawo zazã me ɖa. Ele be nàse alesi tututu atike si wɔa dɔ le wò atike yeye si nètsaka ƒe mililita ɖesiaɖe me gɔme. Míezãa numekuku ƒe didime si le tẽ tsɔ dea dzesi esia. Nu vevitɔ ƒe mɔnu bia be woama atikekui ƒe agbɔsɔsɔme bliboa ɖe atikekui ƒe agbɔsɔsɔme bliboa nu.
Mɔnu Vevitɔ: Peptide mg bliboa / Diluent ƒe ml bliboa = Agbɔsɔsɔme le ml ɖeka me.
Hafi nàwɔ mɔ̃a ŋudɔ la, ehiã be nàwɔ tɔtrɔ ƒe gɔmeɖoanyi sesẽ aɖe. Nugoewo ŋlɔa nusiwo le wo me ɖe miligram (mg) me. Zi geɖe la, atikekuiwo dzidzea agbɔsɔsɔme le microgram (mcg) me. Ele be nàtrɔ unit siawo pɛpɛpɛ. Xexeame katã ƒe dzidzenu gblɔ be miligram 1 sɔ kple microgram 1000. Trɔtrɔ sia tsɔtsɔ de ŋkuɖodzinu me xea mɔ na nudokpɔƒe ƒe dzidzedze ƒe vodada siwo bɔ wu.
Mina míadzro kpɔɖeŋu ƒe nɔnɔme nyui aɖe me. Tsɔe be nugoe aɖe si me lyophilized powder 5mg le le asiwò. Èɖoe be yeatsɔ BAC tsi 2ml atsakae. Mɔnu vevitɔa zazã: 5mg si woma ɖe 2ml me sɔ kple 2.5mg le 1ml ɖesiaɖe me. Emegbe míetrɔa emetsonua wòzua microgram. Dzi 2.5mg ɖe edzi zi gbɔ zi 1000. Ènya azɔ be atike si wɔa dɔ 2500mcg le wò tsi me le mililita ɖesiaɖe me.
Eyome ele be nàɖe agbɔsɔsɔ sia gɔme be wòanye syringe ƒe ʋuʋudedi ƒe dzesiwo. Insulin-style syringe si ƒe kpekpeme nye 1ml si wozãna ɖaa la me unit 100 (tick marks) le. Nu ɖesiaɖe tsi tre ɖi na tsi 0.01ml. Ne 1ml lé 2500mcg la, míemaa 2500 ɖe 100 nu. Esia fia be atike ɖekaɖeka ƒe dzesi ɖesiaɖe si le syringe la dzi la me 25mcg pɛpɛpɛ le atike la me. Ne wò ɖoɖowɔɖi bia be nàzã 250mcg la, àhe tsi la vaseɖe 10-unit ƒe dzesi dzi.
Syringe ƒe akpawo (1ml/100U) . |
Agbɔsɔsɔme si Woɖe (ml) . |
Dose (5mg si wotsaka le 2ml me) . |
Dose (10mg si wotsaka le 2ml me) . |
|---|---|---|---|
10 Units |
0.1 ml |
250 ƒe mcg |
500 mcg |
20 Units |
0.2 ml |
500 mcg ƒe kpekpeme |
1000 ƒe mcg |
50 Units |
0.5 ml |
1250 ƒe mcg |
2500 ƒe mcg |
Akɔntabubu si wotsɔ asi wɔe gblẽa mɔnukpɔkpɔ ɖi na amegbetɔ ƒe vodada. Míele aɖaŋu ɖom vevie be nàzã akɔntabubumɔ̃ siwo ŋu woate ŋu aɖo kpee le Internet dzi siwo wowɔ koŋ na atike siawo. Ŋlɔ wò vial ƒe lolome, diluent ƒe lolome, kple microgram agbɔsɔsɔme si nèdi. Dɔwɔnua aɖe unit xexlẽme si tututu hiã la afia. Digitál akɔntabubumɔ̃ zazã atsɔ alé ŋku ɖe wò akɔntabubu si nètsɔ asi wɔe ŋu zi eve doa ŋusẽ numekuku ƒe ɖoɖowo ƒe mɔnu si me kpododonu mele o.
Nutome ƒe nɔnɔmewoe gblɔa agbenɔƒe si woatsɔ adzrae ɖo. Ele be nàwɔ dzoxɔxɔ ŋuti ɖoɖo sesẽwo ŋudɔ atsɔ akpɔ wò nudzraɖoƒewo ta. Nudzraɖoƒe si nàdzra ɖo do ŋgɔ na gbugbɔgaɖoanyi nɔ te ɖe wò ɣeyiɣiɖoɖo dzi. Ne èdi be yeadzrae ɖo ɣeyiɣi didi ɣleti geɖe la, da tsigoe siwo me tsi le la de nufamɔ̃ si me goglo si ƒe dzoxɔxɔ nye -20°C. Ne wodzrae ɖo ɣeyiɣi kpui aɖe le kwasiɖa ʋɛ aɖewo te la, fridzi si wozãna ɖaa si ƒe dzoxɔxɔ nye 2°C va ɖo 8°C wɔa dɔ nyuie. Gake se siwo wowɔna le gbugbɔgaɖoanyi megbe la trɔna kura. Ne tsi nya ge ɖe nugoa me ko la, ele be tsi si woɖe tso eme la nanɔ nufamɔ̃ me pɛpɛpɛ le dzoxɔxɔ 2°C va ɖo 8°C. Mègatsɔ tsi siwo me tsi le la de tsikpe me o.
Kekeli kple oxidation hea afɔku gãwo vɛ na molecule ƒe blibonyenye. Ultraviolet kekeli gblẽa atikewo ƒe kadodo sesẽwo me kabakaba to foto-oxidation me. Ele be nàtsɔ nugoewo ade nugoe siwo me kekeli mate ŋu age ɖo o me ɣesiaɣi. Ne nugoe tɔxɛwo mele asiwò o la, dzra wo ɖo ɖe teƒe si do viviti kura, abe fridzi ƒe agbalẽdzraɖoƒe ƒe megbe dzogoe ene. Ðe woƒe kekeli si le xɔa me ƒe keklẽ dzi kpɔtɔ alesi nàte ŋui le etsɔtsɔ yi teƒe bubu ƒe ɖoɖoa me.
Nusiwo woate ŋu adzra ɖo ƒe agbenɔƒe ŋutɔŋutɔ gɔmesese xea mɔ na atike siwo gblẽ zazã le vo me. Dɔnɔdzikpɔƒe ƒe kpeɖodziwo na gɔmeɖoanyi si me kɔ nyuie si dzi woato agbɔ agbe. Kekiake peptide siwo wogbugbɔ wɔ to BAC tsi zazã me léa woƒe liƒo ƒe kɔkɔƒe me ɖe asi abe kwasiɖa 3 va ɖo 4 ene. Le ŋkeke 28 lia megbe la, benzyl aha la megate ŋu kpɔa ame ta o. Atikea ŋutɔ dzea gbagbã gɔme, eye megawɔa dɔ kabakaba o. Ŋlɔ ŋkeke si tututu dzi wogbugbɔ ɖo wò nugoewo dzi ɣesiaɣi. Ele be nàtsɔ egbɔkpɔnu ɖesiaɖe si ɖea alilikpo, amadede ƒe tɔtrɔ, alo nusue siwo le tsia dzi fiana la aƒu gbe enumake, ŋkeke ka kee ɖale eŋu o.
Gbugbɔgaɖoanyi dzidzedzetɔe nɔ te ɖe gɔmeɖoanyi etɔ̃ dzi bliboe: vidzidzi blibo, akɔntabubu ƒe akɔntabubu siwo sɔ pɛpɛpɛ, kple alesi wowɔa nu ɖe eŋu tufafatɔe etɔxɛe. Ne wodo kpo nu le nuto siawo dometɔ aɖe me la, egblẽa nu le atike si wotsɔ wɔa atikekui la ƒe wɔwɔme ƒe blibonyenye ŋu. Ne èse vacuum ƒe ŋusẽkpɔɖeamedzi gɔme eye nède bubu alesi atikekui siwo me tsi le ƒe gbagbãƒe ŋu la, àna dedienɔnɔ kple dɔwɔwɔ siaa nadzi ɖe edzi. Diluent tiatia kpɔa ŋusẽ ɖe agbenɔƒe didi dzi vevie, si wɔe be BAC tsi nye dzidzenu si ŋu womeʋlia nya le o na nudokpɔƒe zazã ɣeyiɣi didi.
Afɔɖeɖe siwo kplɔe ɖo enumake bia be nàlé ŋku ɖe wò nudokpɔƒe ƒe nuzazã siwo li fifia ŋu. Tsɔ tsilénu siwo ƒe ɣeyiɣi do vɔ alo atikekui siwo ŋu ɖikeke le la ƒu gbe. Dzɔtsoƒe deŋgɔ, ame etɔ̃lia ƒe dɔlékuiwutikewo dodokpɔ tso nudzrala xɔŋkɔwo gbɔ. Mlɔeba la, zã dzidzenu siwo sɔ pɛpɛpɛ kple dijitaal akɔntabubumɔ̃wo hafi nàyi tsakatsaka ƒe ɖoɖo yeye ɖesiaɖe dzi. Dzadzraɖo nyuie naa kakaɖedzi be nu siwo ŋu kakaɖedzi le, siwo woate ŋu agbugbɔ awɔ ɣesiaɣi.
A: Zi geɖe la, alilikpo dodo fia be wometsɔe ƒu gbe bliboe o, wometia atike si me wodea tsii nyuie o, alo eƒe nyonyome gblẽ. Zi geɖe la, xɔ siwo me tsi mele o gbea tsi si wozãna ɖaa eye wotrɔna zua notsi. Alo, dzoxɔxɔ ƒe tɔtrɔ sesẽwo le eɖoɖo me ate ŋu agblẽ nu le atikekui la ŋu. Ne ʋuʋu blewu meɖe tsi si le eme le miniti blaetɔ̃ megbe o la, tsɔ nugoe la ƒu gbe keŋkeŋ. Alilikpo me egbɔkpɔnuwo nye esiwo womate ŋu agblɔ ɖi o eye womele dedie na wo zazã o.
A: Ao, ele be nàƒo asa na tsi siwo wotsɔna tsia tsikpe me vevie. Tsi ƒe tsikpe kple tsi ƒe ƒuƒu ƒe tsatsam nana tsi ƒe moleculewo kekena hetsia wo nɔewo dzi sesĩe. Dzɔdzɔmeŋusẽ ƒe kekeɖenudɔ sia naa tsikpe ƒe kristalo suesuesuewo dona. Kristal siawo lãa amino acid ƒe kadodo siwo le bɔbɔe la, si gblẽa nu le xɔa ƒe wɔwɔme ŋu vevie. Na nugoe siwo wogbugbɔ wɔ la nanɔ nufamɔ̃ me ɣesiaɣi le dzoxɔxɔ si nye 36°F kple 46°F dome.
A: Ʋuʋu hea nuteɖeamedzi sesẽ vɛ le lãɖeɖe me eye wònana futukpɔna kabakaba le goa me. Mɔ̃ɖaŋununya ƒe nuveviwɔame sia ate ŋu agblẽ protein ƒe kadodowo me, si ana be woƒe dɔwɔwɔ nabu tegbee. Ne èʋuʋui le vo me la, tsɔ nugoe la de fridzi me enumake. Na wòanɔ anyi gaƒoƒo 24 sɔŋ maɖe fu na wò. De dzesi dɔwɔwɔ nyuie si ate ŋu abu le wò numekukuwo me.
A: Le atikewɔnyawo ƒe mɔfiame siwo wozãna ɖaa nu la, tsi si me dɔlékuiwo tsia dɔlékuiwo le nɔa dedie ŋkeke 28 le etutu vɔ megbe. Benzyl aha si nye 0.9% la gblẽna le ɣeyiɣi aɖe megbe ne wodo go ya kple ɖiƒoƒo siwo ƒo xlãe. Le kwasiɖa ene megbe la, megate ŋu ka ɖe edzi be woaxe mɔ ɖe dɔlékuiwo ƒe tsitsi nu o. Tsɔ BAC tsi si woʋu la ƒu gbe ɣesiaɣi le ŋkeke 29 lia dzi.
