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1kits (10Vials) .
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▎ Testagen ƒe Dɔwɔɖoɖo
Dzɔtsoƒe: PepDraw |
Molekyulãwo ƒe Mɔfiame: C 22H 30N 6O6 Molekyulã ƒe Kpekpeme: 474.53g/mol |
▎ Testagen ƒe Numekuku
Nukae nye Testagen ƒe numekuku?
Le egbegbe nugbagbeŋutinunya kple atikewɔwɔ ŋuti numekuku siwo le ŋgɔ yim ta la, peptide siwo wɔa dɔ le nugbagbewo me didi va zu numekuku ƒe mɔfiame vevi aɖe le dɔa me. Abe peptide si wowɔ ene la, Testagen xɔ vevienyenye ɖedzesi aɖe le numekuku me le asixɔxɔ si ate ŋu anɔ eŋu le lãmenugbagbeviwo ƒe dɔwɔwɔ, lãmenugbagbeviwo ƒe gbugbɔgadzɔ, lãmenugbagbeviwo ƒe dɔwɔwɔ ƒe mɔnuwo, kple ŋutilã kple nugbagbeŋusẽ ƒe ɖoɖo bubuwo me ta. Esi Testagen le bioactive peptides ƒe hatsotso me ta la, wowɔe
ƒe amino acid ƒe ɖoɖo siwo ate ŋu akpɔ ŋusẽ ɖe lãmenugbagbeviwo kple molecule ƒe kadodo dzi. Wotrɔ asi le eƒe wɔwɔme ŋu be wòasrɔ̃ nugbagbewo ƒe dɔwɔwɔ siwo le lãmenugbagbeviwo me alo ado alɔ wo, si wɔe be wòaɖe ŋutete afia abe numekuku ƒe nyati ene le molecule biology, biochemistry, kple regenerative research me.
Nɔnɔmetata 1. Nu gbagbe si me lãmenugbagbevi geɖe le ƒe bioregulation system.
Dzɔtsoƒe: MDPI [1] .
Nukae nye Testagen ƒe dɔwɔwɔ ƒe mɔnu?
Lãmenugbagbeviwo ƒe gege ɖe lãme: Numekukuwo ɖee fia be esi wotsɔ Testagen si wotsɔ fluorescein isothiocyanate de dzesii la de HeLa lãmenugbagbeviwo me vɔ la, wokpɔ keklẽ vovovowo le lãmenugbagbeviwo, lãmenugbagbeviwo ƒe nucleus, kple nucleolus me, si ɖee fia be ŋutete le Testagen si be wòage ɖe lãmenugbagbeviwo kple woƒe nucleus me. Ŋutete si le esi be wòato lãmenugbagbeviwo ƒe lãkusiwo kple nuklia ƒe lãkusiwo me ayi lãmenugbagbeviwo ƒe eme ate ŋu atso protein aɖewo siwo tsɔa nu yia lãmenugbagbevi ƒe lãkusi dzi alo lãmenugbagbevi ƒe tsi si le lãme na ame gbɔ. Lãmenugbagbevi ƒe lãkusi menye nusi me tsi mate ŋu age ɖo kura o; tɔdzisasrãwo kple ʋuɖoɖo ƒe mɔ̃wo le eme. Testagen ate ŋu age ɖe lãmenugbagbeviwo me to nuwɔwɔ kple mɔ̃ siawo siwo tsɔa lãwo alo to mɔnu siwo le abe endocytosis ene dzi to lãkusi ƒe tsi si le lãmenugbagbeviwo me zazã me, si aɖo gɔmeɖoanyi na eƒe ŋusẽkpɔɖeamedzi siwo kplɔe ɖo [2]..
Kadodo Tɔxɛ Kple Nucleic Acids: Peptide vovovo siwo me gblẽ o siwo dzɔ tso la ɖea ŋusẽkpɔɖeamedzi vovovowo fiana ɖe 5,6-carboxyfluorescein-labeled deoxyoligonucleotides kple DNA-ethidium bromide complexes ƒe keklẽ dzi. To Stern-Volmer ƒe madzudzɔmadzudzɔe dzidzedze me la, wokpɔe be Testagen, ne wotsɔe sɔ kple peptide kpui bubuwo la, enaa fluorescence tsitsi ƒe seƒe vovovowo le deoxyoligonucleotides siwo wotsɔ fluorescent de dzesii siwo ŋu ka ɖeka kple ka eve le me, le peptide la ƒe wɔwɔme vevitɔ nu. Esia ɖe kadodo tɔxɛ siwo le Testagen kple nucleic acid ƒe wɔwɔmewo dome fia. Ne Testagen bla ɖe nucleic acid ŋu la, ate ŋu ade vovototo nucleotide ƒe ɖoɖo vovovowo dome eye wòade dzesi woƒe cytosine methylation ƒe nɔnɔme gɔ̃ hã. Le kpɔɖeŋu me, edze abe Testagen lɔ̃a kadodo kple deoxyoligonucleotides siwo me CAG ƒe ɖoɖowo le ene. Woateŋu akpɔ kadodo tɔxɛ sia to kadodo siwo menye covalent o abe haidrodzin kadodo kple elektrikŋusẽ ƒe kadodo le amino acid ƒe nugbagbevi siwo le Testagen molecule dzi kple nucleic acid ƒe gɔmeɖoanyi alo phosphate megbeƒuwo dome. Nu tɔxɛ sia na Testagen te ŋu nɔa nucleic acid ƒe nuto aɖewo koŋ me pɛpɛpɛ, si wɔnɛ be wòkpɔa ŋusẽ ɖe dɔwɔwɔwo abe domenyiŋusẽfianu ƒe ɖeɖefia ene dzi [2]..
Epigenetic Regulation of Cellular Genetic Functions: Le ŋutete si le esi be wòabla DNA koŋ ta la, Testagen ƒe kadodo kple DNA le teƒe tɔxɛ aɖe ate ŋu akpɔ lãmenugbagbeviwo ƒe domenyiŋusẽfianu ƒe dɔwɔwɔ dzi le epigenetic ƒe ɖoɖo nu. Epigenetic ƒe ɖoɖowɔwɔ metrɔa DNA ƒe gɔmeɖoanyi ƒe ɖoɖo o ke boŋ ekpɔa ŋusẽ ɖe domenyiŋusẽfianu ƒe ɖeɖefia dzi to DNA ƒe tɔtrɔ (le kpɔɖeŋu me, methylation) kple histone ƒe tɔtrɔ me. Testagen ate ŋu abla ɖe DNA ƒe nuto aɖewo koŋ ŋu, akpɔ ŋusẽ ɖe chromatin ƒe wɔwɔme dzi le nuto mawo me alo axɔ epigenetic regulatory protein factors be woatrɔ asi le domenyiŋusẽfianu ƒe nuŋɔŋlɔ ƒe dɔwɔwɔ ŋu. Domenyiŋusẽfianu ƒe dɔwɔwɔ dzi kpɔkpɔ alea ate ŋu awɔ akpa vevi aɖe le agbe ƒe gɔmedzedze kple nugbagbewo ƒe nɔnɔmetɔtrɔ ƒe gɔmedzedze, si kpena ɖe nugbagbewo ŋu be woaɖɔ domenyiŋusẽfianu ƒe dɔwɔwɔ ɖo pɛpɛpɛ le nuto vovovowo me be woatrɔ ɖe tɔtrɔwo kple agbenɔnɔ ƒe ɖoɖo siwo de blibo ŋu [2] ..
Ŋusẽkpɔɖeamedzi ɖe Dɔlékuiwutikewo ƒe Dɔwɔwɔ Dzi le Dɔnɔ Siwo Mexɔ Dɔlékuiwo O Me: Le atikewɔwɔ ŋuti numekukuwo me la, numekuku siwo wowɔ le dɔnɔ siwo ŋu dɔlékuiwutikewo Mele O (IIIA) ƒe dɔléle si nɔa anyi didi (IIIA) ŋu ɖee fia be le atikewɔwɔ si me womezãa dɔlékuiwo le o ɣleti ɖeka megbe kple atikewɔmɔnu si me Testagen (α1-adrenergic blockers + rectal suppositories siwo me non-steroidal anti-inflammatory drugs) le la, urodynamic parameters nyona ɖe edzi ŋutɔ, prostatic inflammation ƒe agbɔsɔsɔ dzi ɖe kpɔtɔ, eye serum total testosterone ƒe agbɔsɔsɔ dzi ɖe edzi. Esia fia be Testagen ate ŋu aɖɔ lãmenugbagbeviwo ƒe dadasɔ ɖo to mɔnu aɖewo dzi. Mɔnu si ŋu kakaɖedzi le enye be Testagen kpɔa ŋusẽ ɖe dzesimɔ siwo do ƒome kple testosterone ƒe wɔwɔme le Leydig lãmenugbagbeviwo me dzi. Testosterone ƒe wɔwɔme nye dɔ sesẽ aɖe si me enzyme kple dzesi molecule geɖe le. Testagen ate ŋu ana dzesimɔ siwo do ƒome kplii nawɔ dɔ alo axe mɔ na wo to kadodo kple lãmenugbagbeviwo me xɔlawo me, si ana testosterone ƒe wɔwɔme nado ŋgɔ, ana dɔnɔa ƒe lãmenugbagbeviwo ƒe dɔwɔwɔ nanyo ɖe edzi, eye wòaɖe dzesi siwo nye prostatitis si me dɔlékui mele o si nɔa anyi didi dzi akpɔtɔ [3]..
Nukae nye Testagen ƒe dɔwɔwɔ?
Dɔlékui Si Mexɔa Dɔlékuiwo O ƒe Atikewɔwɔ: Aɖuɖɔtoedɔléle si me dɔlékui mele o (IIIA) si nɔa anyi didi, si zi geɖe la, aɖuɖɔtoe ƒe dzesi siwo le ete kpena ɖe eŋu la kpɔa ŋusẽ gbegblẽ ɖe dɔnɔwo ƒe agbenɔnɔ dzi. Testagen wɔa akpa vevi aɖe le nɔnɔme mawo tɔgbe dada me [4] (Niu D, 2023). Numekuku siwo wowɔ tsɔ zã atikewɔwɔmɔnu si me womezãa α1-adrenergic blockers, rectal suppositories kple non-steroidal anti-inflammatory drugs, kple Testagen tsɔ wɔ dɔ ɖedzesiwo le ɣleti ɖeka megbe. Urodynamic parameters nyo ɖe edzi ŋutɔ, si fia be tsinyegoe ƒe dɔwɔwɔ dzi ɖe edzi eye aɖuɖɔtoe ƒe mɔxexeɖedɔléle ƒe dzesi siwo le ete la dzi ɖe kpɔtɔ. Le ɣeyiɣi ma ke me la, dɔdzẽ si le ʋuka me dzi ɖe kpɔtɔ, si na lãmenugbagbeviwo ƒe gbegblẽ dzi ɖe kpɔtɔ. Vevietɔ wu la, testosterone ƒe agbɔsɔsɔ si le ʋumenugbagbevi dzĩ me dzi ɖe edzi. Testosterone le vevie ŋutɔ na ŋutsu ƒe vidzidzi ƒe dɔwɔwɔ nyuie, gbɔdɔdɔ ƒe dɔwɔwɔ, kple lãmenugbagbeviwo ƒe dɔwɔwɔ bliboa me léle ɖe asi, eye eƒe kɔkɔme kpena ɖe ame ŋu be lãmenugbagbeviwo ƒe dɔléle siwo dɔlélea hena vɛ la nyona ɖe edzi [3]..
Lãmenugbagbeviwo ƒe gege ɖe eme kple Nucleic Acid ƒe kadodo: Abe bioactive peptide kpui aɖe ene la, Testagen ɖea lãmenugbagbeviwo ƒe nɔnɔme tɔxɛ siwo nye gɔmeɖoanyi na eƒe atikewɔwɔ me dɔwɔwɔwo fiana [2] (Fedoreyeva LI, 2011). Le HeLa lãmenugbagbeviwo dodokpɔwo me la, wokpɔ keklẽ ɖedzesi aɖe le lãmenugbagbeviwo, nucleus, kple nucleolus me le Testagen si wotsɔ fluorescein isothiocyanate de dzesii vɔ megbe, si ɖe eƒe ŋutete be wòage ɖe lãmenugbagbeviwo kple nucleus me fia. Esia wɔnɛ be Testagen te ŋu gena ɖe lãmenugbagbeviwo me eye wòwɔa nu kple lãmenugbagbeviwo me akpawo, si wɔnɛ be woate ŋu awɔ ɖoɖo ɖe lãmenugbagbeviwo ŋu.
Numekukuwo ɖee fia be peptide kpui vovovo siwo wɔa dɔ le agbe me kpɔa ŋusẽ vovovowo ɖe 5,6-carboxyfluorescein-labeled deoxyribooligonucleotides kple DNA-ethidium bromide complexes ƒe keklẽ dzi. Fluorescence quenching levels, si ƒe dzesi enye Stern-Volmer constants, toa vovo le peptide kpuiwo abe Testagen ene dome le woƒe wɔwɔme gbãtɔ nu ne wole nu wɔm kple deoxyribooligonucleotide siwo wotsɔ fluorescent de dzesii kple ka eve, si fia be kadodo tɔxɛwo le wo kple nucleic acid ƒe wɔwɔmewo dome. Numekuku bubuwo ɖee fia be Testagen lɔ̃a kadodo kple deoxyribooligonucleotides siwo me CAG ƒe ɖoɖowo le. Ŋutete sia si le esi be wòabla nucleic acid ƒe ɖoɖo tɔxɛwo ɖee fia be Testagen ate ŋu aɖɔ lãmenugbagbeviwo ƒe domenyiŋusẽfianu ƒe dɔwɔwɔ ɖo le epigenetic ƒe ɖoɖo nu to DNA ƒe kadodo siwo le teƒe tɔxɛ aɖe me, si awɔ akpa vevi aɖe le domenyiŋusẽfianu ƒe dɔwɔwɔ dzi kpɔkpɔ me. Esia ate ŋu ato atikewɔmɔnu siwo wotu ɖe domenyiŋusẽfianu ƒe ɖoɖowɔwɔ dzi vɛ—le kpɔɖeŋu me, le dɔléle siwo do ƒome kple domenyiŋusẽfianu ƒe ɖeɖefia si mesɔ o me. Togbɔ be womegblɔ alesi wozãnɛ tẽ le atikewɔnyawo me haɖe o hã la, mɔ̃ɖaŋununya ŋuti numekuku siwo wowɔ le lãmenugbagbeviwo kple molecule ƒe ɖoɖowo me fia be woazãe le atikewɔwɔ me le etsɔme.
Nyanuwuwuw
Kpuie ko la, Testagen ɖe atikewɔwɔ ƒe ŋutete fia le prostatitis si menye dɔlékui o si nɔa anyi didi dada me, evɔ eƒe nɔnɔme siwo nye lãmenugbagbeviwo ƒe gege ɖe lãme kple nucleic acid ƒe kadodo kple wo nɔewo na ŋutete le atikewɔwɔ me le mɔ si keke ta wu nu. Ne wogawɔ numekuku geɖe wu la, wole mɔ kpɔm be awɔ akpa vevi aɖe le dɔléle geɖe dada kple atikewɔwɔ ƒe nudede nyawo me me.
Le Agbalẽŋlɔla La Ŋu
Nusiwo míeyɔ ɖe etame la katã nye esiwo ŋu Cocer Peptides wɔ numekuku le, trɔ asi le wo ŋu eye woƒo wo nu ƒu.
Dzɔdzɔmeŋutinunya ƒe Agbalẽŋlɔla
Niu, Dun nye agbalẽnyala xɔŋkɔ aɖe le atikewɔwɔ kple agbeŋutinunya me. Esi wòdo ƒome kple dɔwɔƒe xɔŋkɔwo abe Army Medical University kple University of South , eye wòtsɔa eƒe numekukuwo ɖoa Atikeŋutinunya & Atikeŋutinunya, Lãmenugbagbeviŋutinunya, Dɔlélenutsiŋutete, kple Biochemistry & Molecular Biology ŋu. Tohehe siawo le vevie ŋutɔ le dɔléle ƒe mɔnuwo didi, atike yeyewo dodo ɖe ŋgɔ, kple amegbetɔ ƒe lãmesẽ dodo ɖe ŋgɔ me. Tsɔ kpe ɖe eŋu la, Niu, Dun wɔa numekuku le Cardiovascular System & Cardiology me, eye wòdzroa dzitodzito me dɔlélewo ƒe dzɔdzɔ kple wo dada me. Eƒe dɔwɔwɔ naa nukpɔsusu ƒe gɔmeɖoanyi veviwo kple mɔfiame ŋutɔŋutɔwo hena dzitodzito me dɔlélewo didi le kɔdzi kple wo dada, si ɖe eƒe nunya deto kple ŋusẽkpɔɖeamedzi gbadzaa le atikewɔwɔ ŋuti numekuku me fia.. Niu, Dun is listed in the reference of citation [4].
