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1kits （10Ibikoresho）
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Imiterere ya Testagen Structure
Inkomoko: PepDraw |
Inzira ya molekulari: C 22H 30N 6O.6 Uburemere bwa molekuline: 474.53g / mol |
Research bwa Testagen Ubushakashatsi
Ni ubuhe bushakashatsi bwakozwe na Testagen?
Kuruhande rwiterambere ryibinyabuzima bigezweho nubushakashatsi bwibinyabuzima, ubushakashatsi bwa peptide bioactive bwabaye icyerekezo cyingenzi cyubushakashatsi murwego. Nka peptide ya sintetike, Testagen ifite akamaro gakomeye mubushakashatsi bitewe nagaciro kayo mumikorere ya selile, kuvugurura ingirabuzimafatizo, inzira ya metabolike, hamwe nubundi buryo bwa physiologique na biohimiki. Hafi yicyiciro cya bioactive peptide, Testagen igizwe
ya aside amine ikurikirana ishobora kugira ingaruka kumikoranire ya selile. Imiterere yacyo yagenewe kwigana cyangwa gushyigikira inzira y'ibinyabuzima ya endogenous, bityo ikerekana ubushobozi nk'isomo ry'ubushakashatsi muri biologiya ya molekuline, ibinyabuzima, n'ubushakashatsi bushya.
Igishushanyo 1. Sisitemu ya Bioregulation yibinyabuzima byinshi.
Inkomoko: MDPI [1]
Ni ubuhe buryo bwo gukora bwa Testagen?
Kwinjira mu ngirabuzimafatizo: Ubushakashatsi bwerekanye ko nyuma yo gutera ingirabuzimafatizo za HeLa hamwe na fluorescein isothiocyanate yanditseho Testagen, fluorescence itandukanye yagaragaye muri cytoplazme, nucleus selile, na nucleolus, byerekana ko Testagen ifite ubushobozi bwo kwinjira mu ngirabuzimafatizo no muri nuclei. Ubushobozi bwabwo bwo kunyura mu ngirabuzimafatizo no mu kirere imbere mu ngirabuzimafatizo bishobora kuba bifitanye isano na poroteyine zimwe na zimwe zitwara abantu ku ngirabuzimafatizo cyangwa amazi ya membrane. Ingirabuzimafatizo ntago ari imiterere yuzuye; ikubiyemo imiyoboro hamwe nuburyo bwo gutwara abantu. Testagen irashobora kwinjira mu ngirabuzimafatizo ikorana na poroteyine zitwara abantu cyangwa ikoresheje uburyo busa na endocytose ikoresha amazi ya membrane, igashyiraho urufatiro rw'ingaruka zayo nyuma ..
Imikoranire yihariye na Acide Nucleic: Peptide zitandukanye zidafite inkomoko zerekana ingaruka zitandukanye kuri fluorescence ya 5,6-carboxyfluorescein yanditseho deoxyoligonucleotide hamwe na ADN-etidium bromide. Mugupima imiterere ya Stern-Volmer, byagaragaye ko Testagen, ugereranije nizindi peptide ngufi, itera impamyabumenyi zitandukanye za kuzimya fluorescence mumurongo umwe kandi ufite imirongo ibiri ya fluoresc yanditseho deoxyoligonucleotide, ukurikije imiterere yibanze ya peptide. Ibi birerekana imikoranire yihariye hagati ya Testagen na acide nucleic. Iyo uhuza aside nucleic, Testagen irashobora gutandukanya nucleotide zitandukanye ndetse ikanamenya imiterere ya cytosine. Kurugero, Testagen isa nkaho ihuza na deoxyoligonucleotide irimo CAG ikurikirana. Ihuza ryihariye rishobora kugerwaho hifashishijwe imikoranire idahwitse nka hydrogène ya hydrogène n’imikoranire ya electrostatike hagati y’ibisigisigi bya aside amine kuri molekile ya Testagen hamwe n’ibishingwe cyangwa umugongo wa fosifike ya acide nucleique. Ubu buryo bwihariye butuma Testagen ihinduka neza mukarere ka acide nucleique, bityo bikagira ingaruka kumikorere nka gene imvugo [2].
Epigenetike igenga imikorere ya selile ya selile: Bitewe nubushobozi bwayo bwo guhuza cyane na ADN, imikoranire yihariye ya Testagen na ADN irashobora kugenzura imikorere yimikorere ya selile kurwego rwa epigenetike. Epigenetike igenga ntabwo ihindura urutonde rwa ADN ahubwo igira uruhare mu mvugo ya gene binyuze mu guhindura ADN (urugero, methylation) no guhindura amateka ya histone. Testagen irashobora guhuza uturere tumwe na tumwe twa ADN, ikagira ingaruka ku miterere ya chromatine muri utwo turere cyangwa gushaka ibintu bya poroteyine bigenga poroteyine kugira ngo bihindure ibikorwa byo kwanduza gene. Uku kugenzura ibikorwa bya gene bishobora kuba byaragize uruhare runini mubyiciro byambere byubuzima bwabayeho ndetse nubwihindurize bwibinyabuzima, bifasha ibinyabuzima kugenga neza imiterere ya gene ahantu hatandukanye kugirango ihuze nimpinduka nubuzima bwuzuye [2].
Ingaruka ku mikorere ya Endocrine mu barwayi ba Prostatite idakira idakira: Mu bushakashatsi bw’ubuvuzi, ubushakashatsi bwakozwe ku barwayi barwaye prostatite idakira ya bagiteri (IIIA) bwerekanye ko nyuma y’ukwezi kumwe bavuwe mu buryo bwa conservateur hamwe na gahunda harimo na Testagen (α1-adrenergic blockers + suppositories zirimo steroidal anti-inflammatory) byagabanutse cyane ibipimo bya urodynamic. Ibi birerekana ko Testagen ishobora kugenga uburinganire bwa endocrine binyuze muburyo bumwe. Uburyo bushoboka ni uko Testagen igira ingaruka zerekana inzira zijyanye na synthesis ya testosterone muri selile ya Leydig. Synthesis ya testosterone ninzira igoye irimo enzymes nyinshi hamwe na molekile zerekana. Testagen irashobora gukora cyangwa ikabuza inzira zerekana ibimenyetso bifitanye isano no kwakirwa kwa reseptor selile, bityo bigatera synthesis ya testosterone, kunoza imiterere ya endocrine yumurwayi, no kugabanya ibimenyetso bya prostatite idakira idakira ..
Ni ubuhe buryo bukoreshwa muri Testagen?
Kuvura Prostatite idakira idakira: Prostatite idakira (IIIA), akenshi iherekejwe n'ibimenyetso byo mu nkari zo hasi, bigira ingaruka mbi ku mibereho y'abarwayi. Testagen igira uruhare runini mu kuvura ibintu nk'ibi [4] (Niu D, 2023). Ubushakashatsi bwifashishije uburyo bwo kuvura indwara zifatika zihuza α1-adrenergic blokers, suppositories rectal hamwe n’imiti itari steroidal anti-inflammatory, na Testagen byageze ku musaruro ushimishije nyuma yukwezi kumwe. Ibipimo bya Urodynamic byateye imbere cyane, byerekana imikorere ya micturition no kugabanya ibimenyetso byo guhagarika inkari zo hasi. Hagati aho, intraprostatic inflammation yagabanutse, bigabanya kwangirika kwinyama. Icyingenzi cyane, serumu yuzuye testosterone yiyongereye. Testosterone ni ingenzi cyane mu gukomeza imirimo isanzwe y’imyororokere y’umugabo, imikorere y’imibonano mpuzabitsina, hamwe na metabolism muri rusange, kandi kuzamuka kwayo bifasha kunoza indwara ziterwa na endocrine ziterwa n'indwara [3].
Kwinjira mu ngirabuzimafatizo hamwe na Acide Nucleic Acide: Nka peptide ngufi ya bioaktike, Testagen yerekana imiterere yihariye ya selile igize ishingiro ryubuvuzi bwayo [2] (Fedoreyeva LI, 2011). Mu bushakashatsi bwakozwe na selile ya HeLa, fluorescence yagaragaye cyane muri cytoplazme, nucleus, na nucleolus nyuma yo gukorerwa na fluorescein isothiocyanate yanditseho Testagen, byerekana ubushobozi bwayo bwo kwinjira mu ngirabuzimafatizo no muri nuclei. Ibi bituma Testagen yinjira muri selile kandi igahuza nibice bigize ingirabuzimafatizo, bigafasha imikorere yubuyobozi muri selile.
Ubushakashatsi bwerekanye ko peptide ngufi itandukanye igira ingaruka zitandukanye kuri fluorescence ya 5,6-carboxyfluorescein yanditseho deoxyribooligonucleotide na ADN-etidium bromide. Urwego rwo kuzimya Fluorescence, rwaranzwe na Stern-Volmer ihagaze, iratandukanye muri peptide ngufi nka Testagen bitewe nuburyo bwabo bwibanze iyo ikorana na floresores imwe imwe kandi ikubye kabiri yanditseho deoxyribooligonucleotide, byerekana imikoranire yihariye nuburyo bwa acide nucleique. Ubundi bushakashatsi bwerekana ko Testagen ihuza cyane na deoxyribooligonucleotide irimo CAG ikurikirana. Ubu bushobozi bwo guhuza aside nucleique ikurikirana byerekana ko Testagen ishobora kugenzura imikorere yimikorere ya selile kurwego rwa epigenetike binyuze mumikoranire yihariye ya ADN, ikagira uruhare runini mugutunganya ibikorwa bya gene. Ibi bifite ubushobozi bwo guteza imbere imiti igenga imiti-urugero, mu ndwara zijyanye no kwerekana imiterere idasanzwe. Nubwo amavuriro ataziguye ataratangazwa, ubushakashatsi bwubukanishi kurwego rwa selile na molekile bwerekana ubuvuzi buzaza.
Umwanzuro
Muri make, Testagen yerekanye uburyo bwiza bwo kuvura mu kuvura prostatite idakira ya bagiteri, mu gihe imiterere yayo yinjira mu ngirabuzimafatizo hamwe na aside nucleic itanga imbaraga zishobora gukoreshwa mu buvuzi bwagutse. Hamwe n’ubushakashatsi bwakozwe, biteganijwe ko buzagira uruhare runini mu kuvura indwara nyinshi no kwivuza.
Ibyerekeye Umwanditsi
Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.
Umwanditsi w'ikinyamakuru
Niu, Dun ni intiti ikomeye mubijyanye n'ubuvuzi n'ubumenyi bw'ubuzima. Afatanije n’ibigo bizwi nka kaminuza y’ubuvuzi ya Gisirikare na kaminuza y’Amajyepfo, yibanze ku bushakashatsi bwe kuri Pharmacology & Farumasi, Biologiya Cell, Immunology, na Biochemistry & Molecular Biology. Izi disipuline ni ingenzi mu kuvumbura uburyo bw’indwara, guteza imbere imiti mishya, no kuzamura ubuzima bw’abantu. Byongeye kandi, Niu, Dun akora ubushakashatsi muri sisitemu yumutima nimiyoboro yumutima & Cardiology, akora ubushakashatsi ku gutera no kuvura indwara z'umutima. Ibikorwa bye bitanga urufatiro rukomeye nubuyobozi bufatika bwo gusuzuma indwara no kuvura indwara zifata umutima-mitsi, bikagaragaza ubuhanga bwe bwimbitse ndetse n’uruhare runini yagize mu bushakashatsi bw’ubuvuzi .. Niu, Dun yanditse ku rutonde rwavuzwe [4].
