Na Cocer Peptides 
ukwezi gushize
INGINGO ZOSE N'AMAKURU Y’IBICURUZWA BITANZWE KURI URU RUBUGA BISANZWE KUBONA AMAKURU N'INTEGO Z'UBUREZI.
Ibicuruzwa byatanzwe kururu rubuga bigenewe gusa mubushakashatsi bwa vitro. Mu bushakashatsi bwa vitro (Ikilatini: * mu kirahure *, bisobanura mu bikoresho by'ibirahure) bikorwa hanze y'umubiri w'umuntu. Ibicuruzwa ntabwo ari imiti, ntabwo byemejwe n’ikigo cy’Amerika gishinzwe ibiryo n’ibiyobyabwenge (FDA), kandi ntigomba gukoreshwa mu gukumira, kuvura, cyangwa gukiza indwara iyo ari yo yose y’ubuvuzi, indwara, cyangwa indwara. Birabujijwe rwose n'amategeko kwinjiza ibyo bicuruzwa mumubiri wabantu cyangwa inyamaswa muburyo ubwo aribwo bwose.
Incamake
TB 500, izwi kandi nka Thymosin Beta-4, ni peptide igizwe na acide 43 amine. Ifite uruhare runini mubikorwa bitandukanye bya physiologique mumubiri wumuntu, cyane cyane mugukiza imitsi. TB 500 yabanje gutandukanywa na thymus tissue, kandi ubushakashatsi bwerekanye ko bufite imirimo yingenzi yo kugenzura kwimuka kwimuka, angiogenez, no gusana ingirangingo.
Urebye kuri molekuliyumu, TB 500 idasanzwe ya aminide acide itanga ibikorwa byibinyabuzima byihariye. Imiterere yimiterere yayo ituma ikomeza gukora mumikorere itandukanye ya physiologique, igashyiraho urufatiro rwuruhare rwayo mugukiza imitsi. Iyi peptide ikwirakwizwa cyane mu mubiri, hamwe ninshi igaragara mumitsi yimitsi, byerekana isano ihuza imikorere yimitsi.
Igicapo 1 Kuvura TB4 ikurikira coronary ligation itezimbere imikorere ya myocardial muri vivo. Ikwirakwizwa ryibumoso bugabanya ibice (FS).
Uburyo bukoreshwa na TB 500 butera imitsi gukira
(1) Kwimuka kwakagari hamwe nuburyo bushya
Gutezimbere kwimuka rya myoblast
Nyuma yo gukomeretsa imitsi, kwimuka kwa myoblast ni ngombwa mu gusana ahangiritse. TB 500 irashobora guhuza ibyakirwa byihariye kuri selile, gukora inzira zerekana ibimenyetso, kandi bigatera kwimuka kwa myoblast aho yakomeretse. Ubushakashatsi bwerekanye ko mubushakashatsi bwa vitro selile, kongeraho TB 500 byihutisha cyane umuvuduko wimuka wa myoblast kandi byongera intera yimuka. Ubu buryo bukubiyemo kuvugurura cytoskeleton, kandi TB 500 irashobora kugenga polymerisation na depolymerisation ya actin, bigatuma myoblast igenda neza cyane mumitsi yangiritse kandi igatanga isoko ya selile ikenewe kugirango imitsi isubirane.
Gukora Satelite Gukora no Gutandukanya
Utugingo ngengabuzima ni ingirabuzimafatizo mu ngingo z'imitsi zishobora gukora kandi zigatandukanywa muri myoblast nyuma yo gukomeretsa imitsi, hanyuma igahuza gukora imitsi mishya. TB 500 yongerera ibimenyetso ibimenyetso byo gukora ingirabuzimafatizo, biteza imbere kuva muri leta ituje ikajya muri leta ikwirakwira. Igenga imvugo yibintu bifatika byandikirwa, nka MyoD na Myf5, kugirango bayobore ingirabuzimafatizo zigana itandukaniro rya myoblast. Mu bushakashatsi bw’inyamaswa, gutera inshinge TB 500 mu mitsi yakomeretse byatumye ubwiyongere bugaragara bw’imikorere ya selile ikora, ndetse no kongera ubwinshi nubwiza bwimitsi mitsi mishya yashizweho, byerekana ko igituntu 500 kigira uruhare runini mugutezimbere imitsi ya selile.
(2) Uburyo bwa Angiogenezi
Kwinjiza Imikurire ya Endoteliyale Ikura (VEGF) Kugaragaza
Angiogenezi ni ingenzi cyane mu kugarura imitsi, kuko itanga ogisijeni nintungamubiri ku ngingo zakomeretse mu gihe ikuraho imyanda ya metabolike. TB 500 irashobora gukangura ingirabuzimafatizo n'imitsi ikikije mesenchymal kugirango igaragaze imikurire y'amaraso (VEGF). VEGF ningingo nyamukuru igenga angiogenez, iteza imbere ikwirakwizwa, kwimuka, hamwe na tubule ya selile endothelia selile. Ubushakashatsi bwerekana ko mubice by'imitsi bivurwa na TB 500, mRNA na proteyine byerekana urugero rwa VEGF byazamutse cyane. Iyi induction igerwaho mugukora inzira yihariye yerekana inzira, nka PI3K-Akt na MAPK yerekana inzira. Gukora izi nzira biteza imbere kwanduza no guhindura gene ya VEGF, bityo bikongerera ururenda rwa VEGF no koroshya imitsi mishya y'amaraso, kunoza itangwa ryamaraso kumitsi yakomeretse.
Igishushanyo cya 2 Guhindura muri gahunda yo gusama gene yo gusama, ifatanije no guhura kenshi nimpamvu zo hanze n’imbere, zitangiza impinduka zitandukanye zindwara mumutima ukuze mubuzima bwacu bwa nyuma yo kubyara.
Gutezimbere imiterere yimitsi mishya
Usibye gutera angiogenez, TB 500 igira uruhare runini muguhindura imiterere yimitsi mishya. Igenga imiterere noguhindura matrice idasanzwe (ECM), ituma imiyoboro mishya yamaraso ishobora kwinjiza neza mumyanya ikikije. TB 500 iteza imbere guhuza ibice bya ECM nka kolagen na fibronectine mugihe uringaniza matrix metalloproteinase (MMPs) hamwe na inhibitor za tissue (TIMPs), bikarinda imiyoboro mishya y'amaraso kwangirika no kwangirika gukabije. Mugihe cyanyuma cya angiogenez, iki gikorwa cya TB 500 gifasha kugumana ubusugire nimirimo yimitsi mishya yamaraso, bigatuma imitsi yakomeretse ikomeza kubona intungamubiri zihagije no guteza imbere imitsi.
(3) Uburyo bwo Kurwanya inflammatory na Immune Igenzura
Kubuza kwerekana ibintu byerekana ibintu
Gukomeretsa imitsi bitera igisubizo, kandi gutwika cyane birashobora kwangiza cyane imitsi. Igituntu 500 gifite imiti igabanya ubukana, ikabuza kwerekana ibintu bitandukanye bitera umuriro nka tumorosi nekrosis-α (TNF-α), interleukin-1β (IL-1β), na interleukin-6 (IL-6). Mugihe cyo gutwika, igituntu 500 gishobora guhuza ibyakirwa hejuru yingirangingo z'umubiri, gukora inzira zerekana ibimenyetso birwanya anti-inflammatory inzira, kandi bikabuza kwandukura no guhindura ingirabuzimafatizo za cytokine. Mu buryo bwo gutwika inyamaswa, nyuma yo gukoresha igituntu 500, urwego rwa TNF-α, IL-1β, na IL-6 mu ngingo z’imitsi rwaragabanutse ku buryo bugaragara, kandi kwinjira mu ngirabuzimafatizo na byo byagabanutse. Ibi byerekana ko igituntu 500 kigabanya ubukana nyuma yo gukomeretsa imitsi kubuza kwerekana ibintu bitera umuriro, bityo bigatuma imitsi ikira.
Kugenga imikorere yumubiri
TB 500 irashobora kandi kugenzura imikorere ya selile yumubiri, igateza imbere ubudahangarwa bw'umubiri bufasha gusana ingirangingo. Irashobora guhindura polarisiyasi ya macrophage, ikabatera kuva mubwoko bwa M1-inflammatory-anti-inflammatory na pro-repair M2. M2 yo mu bwoko bwa macrophage irashobora gusohora ibintu bitandukanye bikura hamwe na cytokine, nka insuline imeze nkikura-1 (IGF-1) no guhindura ibintu bikura-β (TGF-β), bifasha guteza imbere ingirabuzimafatizo no gusana. Byongeye kandi, TB 500 irashobora kugenga imikorere ya Lymphocyte T, igahagarika ubudahangarwa bukabije bwumubiri kugirango birinde kwangirika kwimitsi yumubiri. Muguhindura imikorere yumubiri, TB 500 ihuza ubudahangarwa bw'umubiri nyuma yo gukomeretsa imitsi, bityo bigatera gusana imitsi no gukira.
Uruhare rwa TB 500 mugusubirana imitsi
(1) Kwihutisha gusana imitsi
Kugabanya Igihe cyo Gusana
Yaba imitsi ikaze cyangwa umunaniro udashira, TB 500 irashobora kugabanya cyane igihe cyo gusana kwangirika kwimitsi. Mu bushakashatsi bw’inyamaswa, nyuma yo kwangiza imitsi, itsinda ry’ubushakashatsi ryavuwe na TB 500 ryerekanye umuvuduko wihuse wo gukira aho imitsi yangiritse. Isesengura ry’amateka ryerekanye ko itsinda ry’ubushakashatsi ryerekanye imitsi mishya n’imitsi y’amaraso mu gihe gito, kandi kwinjira mu ngirabuzimafatizo na byo byagabanutse vuba. Mu bushakashatsi bw’abantu, ku bakinnyi bafite imitsi y’imitsi iterwa n’imyitozo ikaze, nyuma yo gutera inshinge cyangwa ubuyobozi bwa sisitemu ya TB 500, abarwayi bavuze ko mbere yo kugabanya ububabare bw’imitsi ndetse no kugabanuka gukabije mu gihe gikenewe kugira ngo imikorere y’imitsi ikure, bibafashe gusubira mu myitozo no mu marushanwa vuba.
Kunoza ubwiza bwo gusana
TB 500 ntabwo yihutisha gusa gusana ibikomere byimitsi ahubwo inatezimbere ubwiza bwo gusana. Binyuze muburyo nko guteza imbere kwimuka kwa myoblast, gukora selile ya satelite no gutandukanya, hamwe na angiogenez, TB 500 ituma ingirangingo zimitsi zasanwe muburyo kandi bukora hafi yimitsi isanzwe. Imitsi yo mumitsi yasanwe itunganijwe neza, kandi imbaraga zimitsi yimitsi hamwe no kwihangana bigarurwa neza. Nyuma yo gukoresha igituntu 500 kugirango usane imitsi yakomeretse, itandukaniro ryingufu ntarengwa zandura nigihe cyo kwihanganira umunaniro hagati yimitsi yasanwe kandi idakomeretse ni nto, byerekana ko igituntu 500 kizamura neza ireme ryimvune yimitsi kandi bikagabanya ingaruka ndende zo gukomeretsa imitsi kumikorere ya moteri.
(2) Kugabanya umunaniro wimitsi
Amabwiriza yo Guhindura Ingufu
Umunaniro wimitsi ufitanye isano rya bugufi ningufu ziterwa na metabolism. TB 500 irashobora kugenga imikorere ya metabolisme yingirabuzimafatizo mu mitsi, ikongera imbaraga zo gutanga ingufu bityo bikagabanya umunaniro wimitsi. Itera mitochondial biogenezi n'imikorere, ikongera umubare wa mito-iyambere ya mito-iyambere, igafasha ingirabuzimafatizo gukora guhumeka neza mu kirere kandi ikabyara adenosine triphosphate (ATP). Byongeye kandi, TB 500 irashobora kugenga imikorere yimisemburo igira uruhare muri metabolisme ya glucose na metabolisme ya aside irike, bigahindura imikoreshereze yingufu zingufu. Mugihe c'imyitozo ngororamubiri, TB 500 irashobora gutuma okiside igabanuka ya aside irike, igaha imitsi imbaraga nyinshi, kugabanya aside ya lactique, no gutinda gutangira umunaniro wimitsi.
Oxidative Stress Regulation
Mugihe c'imyitozo ngororamubiri, hakorwa ubwoko bwinshi bwa ogisijeni ikora (ROS), biganisha ku guhagarika umutima, iyi ikaba ari imwe mu mpamvu nyamukuru zitera umunaniro w'imitsi. TB 500 ifite antioxydeant, yongerera imbaraga imisemburo ya antioxydants yo mu nda nka superoxide dismutase (SOD) na glutathione peroxidase (GPx), kugabanya kwirundanya kwa ROS, no kugabanya kwangirika kwa okiside iterwa n'ingirabuzimafatizo z'imitsi. Mu bushakashatsi bw’inyamaswa, inyamaswa zapimwe na TB 500 zerekanye ibimenyetso bigabanya imbaraga za oxydeide mu ngingo z’imitsi ndetse n’umunaniro woroshye ugereranije nitsinda rishinzwe kugenzura nyuma yo gukora imyitozo myinshi. Ubu bushakashatsi bwerekana ko igituntu 500 kigabanya kwangirika kwimitsi yimitsi kandi kigabanya umunaniro wimitsi muguhindura imyuka ya okiside, bityo bikomeza imikorere yimitsi isanzwe.
(3) Guteza imbere imikurire niterambere
Gukangura imitsi ya poroteyine
TB 500 iteza imbere intungamubiri za poroteyine mu gukora mTOR yerekana inzira muri selile. mTOR ni molekile yingenzi igenga intungamubiri za poroteyine, iteza imbere biosynthesis ya ribosomal hamwe nigikorwa cyo gutangiza poroteyine, bityo bikongera umuvuduko wa sintezamubiri ya poroteyine. TB 500 kandi igenga imvugo yibintu byandikirwa, harimo NF-κB, bigira uruhare rutaziguye intungamubiri za poroteyine. Mu bushakashatsi bw’umuco wa vitro bwerekanye ko kongera igituntu 500 byongereye cyane intungamubiri za poroteyine mu ngirabuzimafatizo. Mu bushakashatsi bw’inyamaswa, inyamaswa zavuwe na TB 500 mugihe kirekire zerekanye ubwiyongere bukabije bwimitsi nimbaraga. TB 500 iteza imbere imikurire niterambere mu gutera intungamubiri za poroteyine.
Kugena ubwoko bwimitsi ya fibre ihinduka
Ubwoko bwa fibre fibre igabanijwemo fibre yihuta kandi itinda-buhoro, itandukana mumyitozo ngororamubiri n'ibiranga metabolike. TB 500 irashobora kugenga ubwoko bwimitsi ihindagurika, ikayerekeza ku cyerekezo cyiza cyo kuzamura imikorere ya siporo. Ubushakashatsi bwerekanye ko igituntu 500 giteza imbere imiterere ya fibre iterwa na fibre gahoro gahoro, nka gene yo mu bwoko bwa myosin itinda cyane (MyHC), mu gihe ibuza imvugo ya fibre yihuta.
Gukoresha igituntu 500 mugusubirana imitsi
(1) Gusaba muri siporo
Kwirinda Imvune z'abakinnyi no gukira
Muri siporo ihiganwa, abakinnyi bahura nimyitozo ikomeye nimbaraga nyinshi, bikaviramo gukomeretsa imitsi. Mugutanga igituntu 500 muburyo bukwiye mbere yimyitozo, imitsi irwanya imvune irashobora kongerwa, bikagabanya ibyago byo gukomeretsa nko kunanirwa imitsi no kurwara. Iyo abakinnyi bakomeretse imitsi, gukoresha igituntu 500 mugihe cyo kuvura birashobora kwihutisha gusana imvune, kugabanya igihe cyo gukira, kandi bigafasha abakinnyi gusubira mumarushanwa vuba. Mu birori bimwe byo gusiganwa ku maguru no mu mupira w'amaguru, abakinnyi batangiye kugerageza na TB 500 kugirango bateze imbere imvune kandi bageze ku musaruro mwiza.
Gutezimbere Imikino ngororamubiri
TB 500 irashobora kandi kongera imikorere yimikino igabanya umunaniro wimitsi no guteza imbere imikurire niterambere. Muri siporo yo kwihangana, TB 500 igenga ingaruka ziterwa ningufu za metabolisme hamwe na stress ya okiside irashobora gutinda gutangira umunaniro wimitsi, bityo bikazamura urwego rwo kwihangana. Muri siporo ikomeye, TB 500 itera intungamubiri za poroteyine yimitsi no kugenzura imitsi ya fibre fibre irashobora kongera imbaraga zimitsi nimbaraga ziturika.
(2) Gusaba mubuvuzi bwo gusubiza mu buzima busanzwe
Kuvura ibikomere byimitsi
Mu buvuzi busubiza mu buzima busanzwe, igituntu 500 gishobora gukoreshwa nk'ubuvuzi bujyanye no kuvura imitsi. Ku barwayi bafite ibikomere by'imitsi biterwa n'ihahamuka, kubagwa, cyangwa izindi mpamvu, guhuza imiti isanzwe yo gusubiza mu buzima busanzwe hamwe no gukoresha igituntu 500 bishobora kongera umusaruro wo gusubiza mu buzima busanzwe. Ku barwayi bafite imvune yimitsi nyuma yo kuvunika, gutera inshinge cyangwa uburyo bwa sisitemu ya TB 500 hamwe nubuvuzi bwumubiri hamwe namahugurwa yo gusubiza mu buzima busanzwe bishobora kwihutisha kugarura imbaraga zimitsi n'imikorere, bityo bikagabanya igihe cyo gusubiza mu buzima busanzwe. Abaganga basubiza mu buzima busanzwe barashobora guhuza gahunda yo kuvura igituntu 500 hashingiwe ku miterere yihariye y’umurwayi, harimo urugero, inshuro, n'inzira z'ubuyobozi, kugira ngo bagere ku musaruro mwiza wo gusubiza mu buzima busanzwe.
Igishushanyo 3: Igitekerezo cyo gukoresha molekile ikora mbere yo gukora kugirango igarure imikorere yumubiri mubasaza.
Kuvura imitsi iterwa no kurwara imitsi
Indwara zimwe na zimwe zifata ubwonko, nko gukomeretsa uruti rwumugongo hamwe na amyotrophique latal sclerose (ALS), bishobora gutera imitsi. Igituntu 500 gishobora kugira ingaruka zo kuvura imitsi iterwa n'indwara zifata ubwonko mu guteza imbere ikwirakwizwa ry'imitsi no guhagarika imitsi ya apoptose. Mu bushakashatsi bw’inyamaswa, kubijyanye n’imitsi y’imitsi iterwa n’imitsi, gukoresha igituntu 500 byatumye habaho iterambere ryinshi mu rwego rwa atrophy yimitsi, ubwinshi bwimitsi n'imbaraga byagaruwe igice.
Umwanzuro
TB 500 irashobora guteza imbere imitsi. Binyuze muburyo nko kwimuka kwakagari no kuvuka bushya, angiogenez, hamwe no kurwanya inflammatory no kurwanya indwara, bigira uruhare runini mugusana imitsi, kugabanya umunaniro, no gukura no gutera imbere. Mu rwego rwa siporo, irashobora gufasha abakinnyi kwirinda no kuvura ibikomere byimitsi no kunoza imikorere ya siporo; mu rwego rw'ubuvuzi busubiza mu buzima busanzwe, burashobora gukoreshwa mu kuvura ibikomere byo kuvura imitsi no kuvura imitsi iterwa n'imitsi ifitanye isano n'indwara zifata ubwonko.
Inkomoko
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Ibicuruzwa biboneka mubushakashatsi bukoreshwa gusa:
