Cocer Peptides ye 
ŋlɔe ɣleti 1 enye sia
NYATAKAKAWO KPLE NUSIWO WOTSƆ ƑE NYATAKAKA SIWO KAtã WOÐO LE NYATAKAKADZRAÐOƑE SIA ME LA KPLE NYATAKAKAWO kaka KPLEE NUFIAFIA ƑE TAÐOÐOWO KO.
Wotrɔ asi le atike siwo wona le nyatakakadzraɖoƒe sia ŋu na numekuku le vitro ɖeɖeko. Wowɔa numekuku le vitro (Latingbe me: *le ahuhɔ̃e me*, si gɔmee nye le ahuhɔ̃e me) le amegbetɔ ƒe ŋutilã godo. Atike siawo menye atikewo o, United States ƒe Nuɖuɖu Kple Atike Ŋuti Dɔwɔƒe (FDA) meda asi ɖe wo dzi o, eye mele be woazã wo atsɔ axe mɔ ɖe atikewɔwɔ, dɔléle, alo dɔléle aɖeke nu, adae, alo adae o. Se de se vevie be woatsɔ atike siawo ade amegbetɔ alo lãwo ƒe ŋutilã me le mɔ ɖesiaɖe nu.
Dzadzraɖo peptidewo ɖe ŋutete gã aɖe fia le lãmenugbagbeviwo ɖɔɖɔɖo kple wo gbugbɔgawɔ ƒe akpa dzi. Abe peptide si dzraa nu ɖo ene la, Chonluten wɔa akpa aɖe le lãmenugbagbeviwo ƒe dɔwɔwɔ dzi kpɔkpɔ kple lãmenugbagbeviwo ɖɔɖɔɖo dodo ɖe ŋgɔ me.
Nɔnɔmetata 1 Chonluten ƒe atikewo ƒe wɔwɔme.
Akpa Si Chonluten Wɔna Le Lãmenugbagbeviwo ƒe Adziɖedzi Kple Dzoxɔxɔ Dzi Kpɔkpɔ Me
Lãmenugbagbeviwo ƒe Adziɖedzi Ŋuti Ðoɖowɔwɔ: Chonluten kple peptide siwo kplɔ wo nɔewo ɖo siwo Nufialagã Khavinson de dzesii tso ƒe 1973 me ate ŋu aɖɔ dzidziɖedzi ƒe ɖoɖo veviwo ɖo to tyrosine phosphorylation si mitogen-activated protein kinases le cytoplasm me dzi ɖeɖe kpɔtɔ me. Chonluten ate ŋu akpɔ ŋusẽ ɖe lãmenugbagbeviwo ƒe dzidziɖedzi ƒe ɖoɖoa dzi, ado lãmenugbagbeviwo ƒe mama kple tsitsi ɖe ŋgɔ, si ana lãmenugbagbeviwo ƒe gɔmeɖoanyi si hiã na lãmenugbagbeviwo ɖɔɖɔɖo. Le kpɔɖeŋu me, le abi ƒe dɔyɔɣi la, lãmenugbagbeviwo ƒe dzidziɖedzi nye afɔɖeɖe vevi aɖe le lãkusi yeyewo wɔwɔ me. Chonluten doa nuwɔna sia ɖe ŋgɔ to kinase siwo do ƒome kplii ƒe dɔwɔwɔ dzi kpɔkpɔ me.
Dzoxɔxɔ ƒe Ðoɖowɔwɔ: Chonluten ɖea dɔwɔwɔ ɖedzesi aɖe fiana le dɔdzẽ dzi kpɔkpɔ me. Woɖee tso aɖuɖɔtoe me lãmenugbagbeviwo me, eye wòxea mɔ na tumor necrosis factor (TNF) ƒe wɔwɔ to monocytes siwo wodo go pro-inflammatory bacterial lipopolysaccharide (LPS) le vitro me. Monocytes wɔa akpa vevi aɖe le dɔdzẽ ƒe ŋuɖoɖowo me; ne LPS do ŋusẽ wo la, woɖea cytokines siwo doa alɔ dɔdzẽ abe TNF ene doa goe tsɔ dzea dɔdzẽ gɔme. Chonluten ɖea dɔdzẽ ƒe ŋuɖoɖo ƒe sesẽ dzi kpɔtɔna to mɔxexe ɖe TNF ƒe wɔwɔ nu me. Peptide siwo katã wodo kpɔ ate ŋu axe mɔ ɖe TNF kple pro-inflammatory interleukin-6 (IL-6) cytokines ƒe ɖeɖefia nu le LPS-stimulated terminally differentiated THP-1 cells me, si gaɖe akpa nyui si Chonluten wɔna le inflammatory microenvironment dzi kpɔkpɔ me fia. Le dɔdzẽ ƒe ŋuɖoɖo siwo dɔdzẽ ƒe dɔlélewo alo lãmenugbagbeviwo ƒe gbegblẽ hena vɛ me la, Chonluten ate ŋu aɖe lãmenugbagbeviwo ƒe gbegblẽ bubu si dɔdzẽ hena vɛ dzi akpɔtɔ to mɔnu sia dzi, si ana nɔnɔme nyui aɖe nanɔ anyi na lãmenugbagbeviwo ɖɔɖɔɖo.
Nɔnɔmetata 2 Lãmenugbagbeviwo godo ƒe ʋukawo ƒe xexlẽme to cytofluorimetric numekuku le monocytes (A) kple macrophages (B) dzi.
Chonluten Zazã le Lãmetsi Vovovowo Dzadzraɖo Me
Ŋutilã ƒe Lãmetsiwo Dzadzraɖo: Le ŋutigbalẽ ƒe abi dzadzraɖo gome la, Chonluten ƒe dɔlékuiwutikewo kple lãmenugbagbeviwo ƒe dzidziɖedzi dodo ɖe ŋgɔ ƒe ŋutetewo le vevie ŋutɔ. Esi ŋutigbalẽa nye ŋutinu gãtɔ kekeake le amegbetɔ ƒe ŋutilã me ta la, exɔa abi vovovowo, abe dzobibi kple lãɖeɖe ene bɔbɔe. Dzoxɔxɔ ƒe ŋuɖoɖowo wɔa dɔ kabakaba le ŋutigbalẽ ƒe abixɔxɔ megbe, eye abi si gbɔ eme ate ŋu ahe abi ƒe hayahaya ɖe megbe eye wòana abi nadze gɔ̃ hã. Chonluten ate ŋu axe mɔ na dɔdzẽ ƒe dɔwɔwɔ, aɖe dɔdzẽ ƒe ŋuɖoɖowo dzi akpɔtɔ, eye le ɣeyiɣi ma ke me la, ana ŋutigbalẽ me lãmenugbagbeviwo nadzi ɖe edzi, si ana abi nahaya kabakaba. Ne wotsɔe sɔ kple abiblaka siwo wozãna tsã la, abiblaka yeye siwo me Chonluten le ate ŋu aɖɔ abi ƒe nɔnɔme suewo ɖo nyuie wu, ado ŋutigbalẽ ƒe lãkusiwo ƒe gbugbɔgadzɔ kple edzadzraɖo ɖe ŋgɔ, ate ŋu aɖe abi ƒe dzɔdzɔ dzi akpɔtɔ, eye wòana ŋutigbalẽa ɖɔɖɔɖo ƒe nyonyome nanyo ɖe edzi.
Nɔnɔmetata 3 Lãmenugbagbevi–Lãmenugbagbeviwo ƒe Adhesion Dodokpɔ: Confocal microscopy ƒe agbɔsɔsɔme kple ICAM-1 Western Blot numekuku.
Ƒumeŋuɖui ƒe lãkusiwo dzadzraɖo: Le ƒunukpeƒewo dzadzraɖo gome la, togbɔ be numekuku siwo wowɔ fifia meƒo nu tso Chonluten zazã le ƒunukpeƒewo dzadzraɖo me ŋu tẽ o hã la, peptide mawo tɔgbe siwo wotsɔ dzraa ƒunukpeƒewo ɖo la ɖe emetsonu nyuiwo fia. Peptide B2A si wowɔ ate ŋu ana lãmenugbagbeviwo nato vovo azu chondrocytes eye wòana ƒunukpeƒe siwo gblẽ ƒe dzadzraɖo nanyo ɖe edzi le sisiblisiwo me. Le nukpɔsusu nu la, chonluten, si te ŋu ɖɔa lãmenugbagbeviwo ƒe dzidziɖedzi kple dɔdzẽ ɖo hã ate ŋu awɔ akpa aɖe le ƒunukpeƒewo ɖɔɖɔɖo me. Le ƒunukpeƒetetedɔ megbe la, teƒea ƒe dɔdzẽ dzɔna, eye Chonluten ate ŋu axe mɔ ɖe dɔdzẽ nu, si wɔnɛ be eƒe nugbegblẽ le ƒunukpeƒewo ŋu dzi ɖena kpɔtɔna. Eƒe lãmenugbagbeviwo ƒe dzidziɖedzi dodo ɖe ŋgɔ ate ŋu ana ƒunukpeƒeŋusẽ ƒe dzidziɖedzi kple vovototodedeameme nanɔ bɔbɔe, ado ƒunukpeƒeŋusẽ ƒe wɔwɔme ɖe ŋgɔ, eye wòato esia me ana ƒunukpeƒeŋusẽ ƒe lãkusiwo naɖɔ ɖo.
Nɔnɔmetata 4 Ŋusẽ si B2A kpɔna ɖe ƒumeŋuɖui si MIA hena vɛ dzi le sisiblisiwo ƒe klo ƒe ƒunukpeƒe.
Ƒumeŋuɖuiwo dzadzraɖo: Le ƒumeŋuɖuiwo dzadzraɖo gome la, numekuku siwo wowɔ fifia ku ɖe peptide kple biomaterials ƒomevi bubuwo koŋ ŋu. PCL scaffold si wowɔ si wotsɔ peptide siwo srɔ̃a kolagen si nye GFOGER zazã doa ƒu ƒe wɔwɔ ɖe ŋgɔ le sisiblisi ƒe ƒu ƒe akpa aɖewo ƒe gbɔdzɔgbɔdzɔ siwo ƒe lolome le vevie me; parathyroid hormone derivatives PTHrP-1 si wotsɔ seke bla ɖe RADA16-I-formed self-assembled peptides P1R16 ŋu ɖea dɔwɔwɔwo abe lãmenugbagbeviwo ƒe dzidziɖedzi dodo ɖe ŋgɔ, vovototodedeameme si wofia mɔe, ƒu ƒe gbugbɔgaɖɔɖo, kple ʋukawo ƒe dɔwɔwɔ ɖe ŋgɔ ene fiana, si ɖea ŋutete gã aɖe fiana le ƒumeŋuɖui ƒe mɔ̃ɖaŋununya me le ƒu ƒe gbagbã alo ƒu gbagbãwo dzadzraɖo me. Chonluten ƒe dɔlékuiwutike kple lãmenugbagbeviwo ƒe dzidziɖedzi dodo ɖe ŋgɔ ɖea dɔdzẽ ƒe nugbegblẽ le ƒumeŋuɖui ŋu dzi kpɔtɔna le ƒuwo ɖɔɖɔɖo ƒe gɔmedzedze, edoa ƒumeŋuɖuiwo ƒe dzidziɖedzi ɖe ŋgɔ, eye wòɖoa gɔmeɖoanyi na ƒumeŋuɖuiwo ƒe gbugbɔgadzɔ.
Nyanuwuwuw
Kpuie ko la, Chonluten, abe peptide si dzraa nu ɖo ene, ɖea ŋusẽkpɔɖeamedzi veviwo fiana le lãmenugbagbeviwo ƒe dzidziɖedzi kple dɔdzẽ dzi kpɔkpɔ me. Eƒe dɔwɔwɔ ƒe mɔnu kple numekuku siwo ŋu woke ɖo le peptide bubu siwo sɔ kple wo nɔewo ŋu la xɔ asixɔxɔ le eŋudɔwɔwɔ le lãmenugbagbeviwo ɖɔɖɔɖo ƒe akpa dzi, siwo dometɔ aɖewoe nye ŋutigbalẽ, ƒunukpeƒetetedɔ, kple ƒu.
Nusiwo ŋu woke ɖo
[1] Avolio F, Martinotti S, Khavinson V. K., kple ame bubuwo. Peptides Siwo Kpɔa Dzidziɖedzi ƒe Dɔwɔna Kple Dzoxɔxɔ Mɔwo Dzi le Monocyte/Macrophage THP-1 Lãmenugbagbeviwo Me[J]. Dukɔwo Dome Molekyulãŋutinunya ƒe Agbalẽ, 2022,23 (7).DOI: 10.3390 / ijms23073607.
[2] Lin X, Shanmugasundaram S, Liu Y, kple ame bubuwo. B2A peptide hea chondrogenic vovototodedeameme vɛ le vitro eye wòdoa ŋusẽ ƒunukpeƒetetedɔ ɖɔɖɔɖo le sisiblisiwo me[J]. Ƒumeŋutinunya ƒe Numekuku ƒe Agbalẽ, 2012,30 (8): 1221-1228.DOI: 10.1002 / jor.22078.
[3] Wojtowicz A. M., Shekaran A., Oest M. E., kple ame bubuwo. Wotsɔ collagen-mimetic peptide GFOGER tsyɔ biomaterial scaffolds dzi hena ƒu ƒe gbagbãwo dzadzraɖo[J]. Nu gbagbewo, 2010, 31 (9): 2574-2582.DOI: 10.1016 / j. nugbagbewo.2009.12.008.
Adzɔnu si li na numekuku zazã ɖeɖeko:
