Na Cocer Peptides 
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INGINGO ZOSE N'AMAKURU Y’IBICURUZWA BITANZWE KURI URU RUBUGA BISANZWE KUBONA AMAKURU N'INTEGO Z'UBUREZI.
Ibicuruzwa byatanzwe kururu rubuga bigenewe gusa mubushakashatsi bwa vitro. Mu bushakashatsi bwa vitro (Ikilatini: * mu kirahure *, bisobanura mu bikoresho by'ibirahure) bikorwa hanze y'umubiri w'umuntu. Ibicuruzwa ntabwo ari imiti, ntabwo byemejwe n’ikigo cy’Amerika gishinzwe ibiryo n’ibiyobyabwenge (FDA), kandi ntigomba gukoreshwa mu gukumira, kuvura, cyangwa gukiza indwara iyo ari yo yose y’ubuvuzi, indwara, cyangwa indwara. Birabujijwe rwose n'amategeko kwinjiza ibyo bicuruzwa mumubiri wabantu cyangwa inyamaswa muburyo ubwo aribwo bwose.
Peptide yo gusana yerekanye imbaraga zikomeye murwego rwo gusana ingirabuzima fatizo. Nka peptide yo gusana, Chonluten igira uruhare mukugenzura ibikorwa bya selile no guteza imbere gusana ingirangingo.
Igishushanyo 1 Imiterere yimiti ya Chonluten.
Uruhare rwa Chonluten mugukwirakwiza selile no kugenzura umuriro
Amabwiriza yo gukwirakwiza selile: Chonluten hamwe nuruhererekane rwa peptide yagaragajwe na Porofeseri Khavinson kuva mu 1973 birashobora kugena uburyo bw’ikwirakwizwa ry’imyororokere mu kongera fosifori ya tirozine ya fosifora ya poroteyine ya poroteyine ikora muri cytoplazme. Chonluten irashobora guhindura uburyo bwo gukwirakwiza ingirabuzimafatizo, igatera amacakubiri no gukura, bityo igatanga umusingi ukenewe wa selile yo gusana ingirangingo. Kurugero, mugihe cyo gukira ibikomere, gukwirakwiza selile nintambwe yingenzi muburyo bushya bwimitsi. Chonluten iteza imbere iki gikorwa muguhuza ibikorwa bya kinase bifitanye isano.
Amabwiriza yo gutwika: Chonluten yerekana imikorere igaragara mugutunganya umuriro. Bikomoka ku ngirabuzimafatizo ya epiteliyale, ibuza kubyara ikibyimba cya nérosose (TNF) na monocytes ihura na bagiteri yitwa lipopolysaccharide (LPS) muri vitro. Monocytes igira uruhare runini mubisubizo byumuriro; iyo bashutswe na LPS, barekura cytokine pro-inflammatory nka TNF kugirango batangire gutwika. Chonluten igabanya ubukana bwibisubizo byokubuza umusaruro wa TNF. Peptide zose zapimwe zirashobora kubuza imvugo ya TNF na por-inflammatory interleukin-6 (IL-6) cytokine muri LPS-iterwa na selile THP-1 itandukanijwe, bikagaragaza uruhare runini rwa Chonluten mukugenzura ibidukikije byangiza. Mubisubizo byokongoka biterwa nindwara ziterwa no kwangirika kwinyama, Chonluten irashobora kugabanya iyangirika ryinyama zatewe no gutwikwa hakoreshejwe ubu buryo, bigatuma habaho ibidukikije byiza byo gusana ingirangingo.
Igishushanyo cya 2 Ibibyimba bidasanzwe bibarwa binyuze muri cytofluorimetric isesengura kuri monocytes (A) na macrophage (B).
Porogaramu ya Chonluten muburyo butandukanye bwo gusana
Gusana imyenda y'uruhu: Mu rwego rwo gusana ibikomere by'uruhu, imiti ya Chonluten yo kurwanya no gukwirakwiza ingirabuzimafatizo ifite akamaro kanini. Nkurugingo runini mumubiri wumuntu, uruhu rushobora kwibasirwa nibikomere bitandukanye, nko gutwika no gukata. Ibisubizo bitwika bikora vuba nyuma yo guhahamuka kwuruhu, kandi gutwika cyane birashobora gutinza gukira ibikomere ndetse bikanatera inkovu. Chonluten irashobora kubuza kurekura ibintu bitera umuriro, kugabanya ibisubizo byokongoka, kandi icyarimwe igatera ikwirakwizwa ryingirangingo zuruhu, bityo kwihutisha gukira ibikomere. Ugereranije no kwambara ibikomere gakondo, imyambarire mishya irimo Chonluten irashobora kugenga neza ibidukikije bikomeretsa ibikomere, bigatera imbaraga kuvugurura uruhu no gusana, bishobora kugabanya inkovu, no kuzamura ireme ryuruhu.
Igishushanyo cya 3 Akagari - Akagari ko gufatira hamwe: Kugereranya microscopi ya Confocal hamwe na ICAM-1 Isesengura ryiburengerazuba.
Gusana ingirangingo za karitsiye: Kubijyanye no gusana ingirabuzimafatizo, nubwo ubushakashatsi buriho butigeze bukemura ikibazo cya Chonluten mugusana karitsiye, peptide yo gusana yerekanye ibisubizo bitanga icyizere. Peptide ya peptide B2A irashobora gutera ingirabuzimafatizo gutandukanya chondrocytes no kongera gusana karitsiye yangiritse ku mbeba. Chonluten, hamwe nubushobozi bwayo bwo kugenzura ikwirakwizwa ryingirabuzimafatizo no gutwika, mubyukuri birashobora no kugira uruhare mugusana karitsiye. Nyuma yo gukomeretsa kwa karitsiye, gutwika kwaho, kandi Chonluten irashobora guhagarika umuriro, bikagabanya kwangirika kwa chondrocytes. Iterambere ryayo ryo gukwirakwiza selile irashobora koroshya ikwirakwizwa rya chondrocyte no gutandukana, guteza imbere synthesis ya matrise ya karitsiye, bityo bikagera no gusana ingirangingo.
Igicapo 4 Ingaruka za B2A kuri MIA iterwa na osteoarthritis mu ivi ryimbeba.
Gusana ingirangingo z'amagufa: Mu rwego rwo gusana ingirangingo z'amagufwa, ubushakashatsi buriho bwibanze ahanini ku bundi bwoko bwo gusana peptide na biomaterial. Ikoreshwa rya sintetike ya PCL isizwe hamwe na peptide ya kolagen-yigana peptide GFOGER iteza imbere amagufwa mumbeba zikomeye-zifite ubunini bw'amagufwa; imisemburo ya parathiyide ikomoka kuri PTHrP-1 yometse kuri RADA16-I yakozwe na peptide P1R16 yerekana imikorere nko guteza imbere ikwirakwizwa ry'uturemangingo, gutandukanya icyerekezo, kuvugurura amagufwa, no kuvura imitsi, byerekana imbaraga zikomeye zo gukora ingirangingo z'amagufwa mu gusana inenge cyangwa kuvunika. Ingaruka za Chonluten zo kurwanya no gukwirakwiza ingirabuzimafatizo zigabanya kwangirika kwangirika kwinyama zamagufwa mugihe cyambere cyo gusana amagufwa, guteza imbere ikwirakwizwa rya osteoblast, no gushyiraho urufatiro rwo kuvugurura amagufwa.
Umwanzuro
Muri make, Chonluten, nka peptide yo gusana, yerekana ingaruka zikomeye muguhuza ikwirakwizwa ry'uturemangingo no gutwika. Uburyo bwibikorwa nubushakashatsi bwakozwe kubindi bisa na peptide yo gusana bifite agaciro gakoreshwa mubijyanye no gusana ingirangingo, harimo uruhu, karitsiye, n'amagufwa.
Reba
[1] Avolio F, Martinotti S, Khavinson VK, n'abandi. Peptide igenga ibikorwa byogukwirakwiza n'inzira zitwika muri Monocyte / Macrophage THP-1 Umurongo w'akagari [J]. Ikinyamakuru mpuzamahanga cya siyansi yubumenyi, 2022,23 (7) .DOI: 10.3390 / ijms23073607.
[2] Lin X, Shanmugasundaram S, Liu Y, n'abandi. B2A peptide itera itandukaniro rya chondrogeneque muri vitro kandi ikanongera gusana karitsiye mu mbeba [J]. Ikinyamakuru cy’ubushakashatsi bw’amagufwa, 2012,30 (8): 1221-1228.DOI: 10.1002 / jor.22078.
[3] Wojtowicz AM, Shekaran A, Oest ME, n'abandi. Gupfundikanya ibiti bya biomaterial hamwe na peptide ya kolagen-mimetike GFOGER yo gusana amagufwa [J]. Ibinyabuzima, 2010,31 (9): 2574-2582.DOI: 10.1016 / j.ibinyabuzima.2009.12.008.
Ibicuruzwa biboneka mubushakashatsi bukoreshwa gusa:
