Los ntawm Cocer Peptides 
1 month ago
Tag nrho cov kab lus thiab cov ntaub ntawv ntawm cov khoom lag luam muab rau ntawm lub vev xaib no tsuas yog rau cov ntaub ntawv tshaj tawm kev tshaj tawm thiab cov hom phiaj kev kawm.
Cov khoom muab rau hauv lub vev xaib no yog npaj tshwj xeeb rau kev tshawb fawb hauv vitro. Kev tshawb fawb hauv vitro (Latin: * hauv iav *, lub ntsiab lus hauv iav) yog ua los ntawm tib neeg lub cev. Cov khoom no tsis yog tshuaj, tsis tau txais kev pom zoo los ntawm US Food and Drug Administration (FDA), thiab yuav tsum tsis txhob siv los tiv thaiv, kho, lossis kho txhua yam mob, kab mob, lossis mob. Nws raug txwv nruj raws li txoj cai los qhia cov khoom no rau hauv tib neeg lossis tsiaj lub cev hauv txhua daim ntawv.
Kho peptides tau pom muaj peev xwm tseem ceeb hauv kev kho cov ntaub so ntswg thiab rov tsim dua tshiab. Raws li kev kho peptide, Chonluten plays lub luag haujlwm hauv kev tswj hwm kev ua haujlwm ntawm tes thiab txhawb kev kho cov ntaub so ntswg.
Daim duab 1 Cov qauv tshuaj ntawm Chonluten.
Lub luag haujlwm ntawm Chonluten hauv Cell Proliferation thiab Inflamation Regulation
Cell Proliferation Regulation: Chonluten thiab ntau cov peptides txheeb xyuas los ntawm xibfwb Khavinson txij li xyoo 1973 tuaj yeem tswj cov qauv kev loj hlob tseem ceeb los ntawm kev nce tyrosine phosphorylation ntawm mitogen-activated protein kinases hauv cytoplasm. Chonluten tuaj yeem cuam tshuam cov txheej txheem ntawm cov cell proliferation, txhawb kev faib cell thiab kev loj hlob, yog li muab lub hauv paus tsim nyog cellular rau cov ntaub so ntswg kho. Piv txwv li, thaum kho qhov txhab, cell proliferation yog ib kauj ruam tseem ceeb hauv kev tsim cov ntaub so ntswg tshiab. Chonluten txhawb cov txheej txheem no los ntawm kev tswj cov haujlwm ntawm cov kinases.
Kev Tswj Kabmob: Chonluten qhia tau hais tias muaj txiaj ntsig zoo hauv kev tswj kev mob. Muab los ntawm bronchial epithelial hlwb, nws inhibits zus tau tej cov qog necrosis factor (TNF) los ntawm monocytes raug rau pro-inflammatory kab mob lipopolysaccharide (LPS) nyob rau hauv vitro. Monocytes ua lub luag haujlwm tseem ceeb hauv cov lus teb inflammatory; Thaum txhawb nqa los ntawm LPS, lawv tso tawm cov cytokines pro-inflammatory xws li TNF los pib mob. Chonluten txo qhov kev siv ntawm inflammatory teb los ntawm inhibiting TNF ntau lawm. Txhua qhov kuaj peptides tuaj yeem cuam tshuam qhov kev qhia ntawm TNF thiab pro-inflammatory interleukin-6 (IL-6) cytokines hauv LPS-stimulated terminally differentiated THP-1 hlwb, ntxiv qhia Chonluten lub luag hauj lwm zoo hauv kev tswj cov kab mob microenvironment. Nyob rau hauv cov lus teb inflammatory tshwm sim los ntawm inflammatory kab mob los yog cov ntaub so ntswg puas, Chonluten tuaj yeem txo cov ntaub so ntswg puas tsuaj ntxiv los ntawm o los ntawm cov txheej txheem no, tsim kom muaj ib puag ncig zoo rau kev kho cov ntaub so ntswg.
Daim duab 2 Extracellular vesicle suav los ntawm cytofluorimetric tsom xam ntawm monocytes (A) thiab macrophages (B).
Daim ntawv thov ntawm Chonluten nyob rau hauv txawv cov ntaub so ntswg kho
Kho daim tawv nqaij daim tawv nqaij: Nyob rau hauv daim tawv nqaij kho qhov txhab, Chonluten's anti-inflammatory thiab cell proliferation-txhawb zog yog tseem ceeb heev. Raws li lub cev loj tshaj plaws nyob rau hauv tib neeg lub cev, daim tawv nqaij raug mob ntau yam, xws li kub hnyiab thiab txiav. Inflammatory teb yog sai sai tom qab ntawm daim tawv nqaij raug mob, thiab ntau o tuaj yeem ncua lub qhov txhab zoo thiab txawm ua rau caws pliav. Chonluten tuaj yeem cuam tshuam qhov kev tso tawm ntawm cov kab mob inflammatory, txo cov lus teb inflammatory, thiab ib txhij txhawb kev loj hlob ntawm daim tawv nqaij, yog li ua kom lub qhov txhab zoo. Piv nrog rau cov khaub ncaws hnav tsoos tsho, hnav khaub ncaws tshiab uas muaj Chonluten tuaj yeem tswj hwm lub qhov txhab microenvironment zoo dua, txhawb nqa cov ntaub so ntswg rov tsim dua tshiab thiab kho dua tshiab, tuaj yeem txo qis kev tsim caws pliav, thiab txhim kho cov tawv nqaij zoo.
Daim duab 3 Cell-Cell Adhesion Assay: Confocal microscopy quantification thiab ICAM-1 Western Blot analysis.
Kev kho cov pob txha mos: Hais txog kev kho pob txha pob txha, txawm hais tias kev tshawb fawb tam sim no tsis tau hais ncaj qha rau daim ntawv thov Chonluten hauv kev kho pob txha mos, zoo li peptides kho tau pom tias muaj txiaj ntsig zoo. Synthetic peptide B2A tuaj yeem tsim cov qia hlwb kom sib txawv rau hauv chondrocytes thiab txhim kho cov pob txha puas hauv nas. Chonluten, nrog nws lub peev xwm los tswj cov cell proliferation thiab o, theoretically kuj tuaj yeem pab kho pob txha mos. Tom qab cov pob txha raug mob, qhov mob hauv zos tshwm sim, thiab Chonluten tuaj yeem txo qis qhov mob, txo nws txoj kev puas tsuaj rau chondrocytes. Nws txhawb kev loj hlob ntawm tes yuav pab txhawb chondrocyte proliferation thiab sib txawv, txhawb cov synthesis ntawm pob txha mos matrix, thiab yog li ua tiav cov pob txha pob txha.
Daim duab 4 Cov nyhuv ntawm B2A ntawm MIA-induced osteoarthritis nyob rau hauv lub hauv caug sib koom ntawm nas.
Kev kho pob txha: Hauv kev kho pob txha pob txha, kev tshawb fawb tam sim no feem ntau tsom rau lwm hom kho peptides thiab biomaterials. Kev siv cov hluavtaws PCL scaffold coated nrog collagen-mimicking peptides GFOGER txhawb kev tsim cov pob txha hauv nas tseem ceeb-loj segmental pob txha tsis xws luag; Parathyroid hormone derivatives PTHrP-1 anchored rau RADA16-I-tsim nws tus kheej-assembled peptides P1R16 nthuav tawm cov haujlwm xws li txhawb cov qia cell proliferation, qhia kev sib txawv, pob txha kho, thiab vascularization, uas qhia tau zoo heev rau cov pob txha cov ntaub so ntswg engineering hauv kev kho cov pob txha tsis xws luag lossis pob txha. Chonluten's anti-inflammatory thiab cell proliferation-txhawb cov teebmeem mitigate inflammatory kev puas tsuaj rau cov pob txha cov ntaub so ntswg nyob rau hauv thaum ntxov ua sawv ntawm cov pob txha kho, txhawb osteoblast proliferation, thiab nteg lub hauv paus rau cov pob txha regeneration.
Xaus
Hauv cov ntsiab lus, Chonluten, raws li kev kho peptide, nthuav tawm cov teebmeem tseem ceeb hauv kev tswj cov cell proliferation thiab o. Nws cov txheej txheem ntawm kev nqis tes ua thiab kev tshawb fawb tshawb pom ntawm lwm yam zoo sib xws kho peptides tuav daim ntawv thov nqi hauv kev kho cov ntaub so ntswg, suav nrog tawv nqaij, pob txha, thiab pob txha.
Cov ntaub ntawv
[1] Avolio F, Martinotti S, Khavinson VK, et al. Peptides Regulating Proliferative Activity thiab Inflammatory Pathways in Monocyte/Macrophage THP-1 Cell Line[J]. International Journal of Molecular Sciences, 2022,23(7).DOI:10.3390/ijms23073607.
[2] Lin X, Shanmugasundaram S, Liu Y, et al. B2A peptide induces chondrogenic sib txawv hauv vitro thiab txhim kho pob txha mos hauv nas[J]. Phau ntawv Journal of Orthopedic Research, 2012, 30(8): 1221-1228.DOI: 10.1002/jor.22078.
[3] Wojtowicz AM, Shekaran A, Oest ME, et al. Txheej ntawm biomaterial scaffolds nrog cov collagen-mimetic peptide GFOGER rau kev kho pob txha tsis xws luag [J]. Biomaterials, 2010,31(9):2574-2582.DOI:10.1016/j.biomaterials.2009.12.008.
Cov khoom muaj rau kev tshawb fawb siv nkaus xwb:
