Bya Cocer Peptides 
omwezi gumu emabega
EBINTU BYONNNA N’AMAWULIRE GW’EBINTU EBIweereddwa KU MUTINDO GUNO BIKOZESEBWA KU KUSASANYA AMAWULIRE N’EBIKOLWA BY’OKUSOMESA.
Ebintu ebiweereddwa ku mukutu guno bigendereddwamu kunoonyereza mu kisenge (in vitro research) byokka. Okunoonyereza mu kisenge (in vitro research) (Olulattini: *mu ndabirwamu*, ekitegeeza mu bikozesebwa mu ndabirwamu) kukolebwa ebweru w’omubiri gw’omuntu. Ebintu bino si bya ddagala, tebikkiriziddwa kitongole kya Amerika ekivunaanyizibwa ku by’emmere n’eddagala (FDA), era tebirina kukozesebwa kuziyiza, kujjanjaba oba kuwonya mbeera yonna ya bujjanjabi, bulwadde oba bulwadde. Kikugirwa nnyo mu mateeka okuyingiza ebintu bino mu mubiri gw’omuntu oba ebisolo mu ngeri yonna.
Repair peptides ziraga obusobozi obw’amaanyi mu kisaawe ky’okuddaabiriza n’okuzza obuggya ebitundu by’omubiri. Nga peptide eddaabiriza, Chonluten ekola kinene mu kulungamya emirimu gy’obutoffaali n’okutumbula okuddaabiriza ebitundu by’omubiri.
Ekifaananyi 1 Ensengekera y’eddagala lya Chonluten.
Omulimu gwa Chonluten mu Kukula kw’obutoffaali n’okulungamya okuzimba
Okulungamya okukula kw’obutoffaali: Chonluten n’omuddirirwa gwa peptides ogwazuulibwa Professor Khavinson okuva mu 1973 bisobola okulungamya enkola enkulu ez’okukula nga byongera ku tyrosine phosphorylation ya mitogen-activated protein kinases mu cytoplasm. Chonluten esobola okufuga enkola y’okukula kw’obutoffaali, n’etumbula okugabanya n’okukula kw’obutoffaali, bwe kityo n’ewa omusingi gw’obutoffaali ogwetaagisa okuddaabiriza ebitundu. Ng’ekyokulabirako, mu kiseera ky’okuwona ebiwundu, okukula kw’obutoffaali ddaala ddene nnyo mu kutondebwa kw’ebitundu ebipya. Chonluten etumbula enkola eno ng’etereeza emirimu gya kinases ezikwatagana.
Okulungamya okuzimba: Chonluten eraga obulungi obw’ekitalo mu kulungamya okuzimba. Eva mu butoffaali bw’omubiri obuyitibwa bronchial epithelial cells, eziyiza okukola tumor necrosis factor (TNF) okukolebwa monocytes ezikwatibwa pro-inflammatory bacterial lipopolysaccharide (LPS) mu vitro. Monocytes zikola kinene nnyo mu kuddamu kw’okuzimba; bwe zisikirizibwa LPS, zifulumya obutoffaali obuyamba okuzimba nga TNF okutandika okuzimba. Chonluten ekendeeza ku maanyi g’okuddamu kw’okuzimba ng’eziyiza okukola TNF. Peptides zonna ezikebereddwa zisobola okuziyiza okwolesebwa kwa TNF ne pro-inflammatory interleukin-6 (IL-6) cytokines mu LPS-stimulated terminally differentiated THP-1 cells, ekyongera okulaga omulimu omulungi Chonluten gw’akola mu kulungamya microenvironment okuzimba. Mu kuddamu kw’okuzimba okuleetebwa endwadde z’okuzimba oba okwonooneka kw’ebitundu, Chonluten esobola okukendeeza ku kwonooneka kw’ebitundu okusingawo okuva mu kuzimba ng’eyita mu nkola eno, okutondawo embeera ennungi ey’okuddaabiriza ebitundu.
Ekifaananyi 2 Omuwendo gw’ebikuta eby’ebweru w’obutoffaali okuyita mu kwekenneenya kwa cytofluorimetric ku monocytes (A) ne macrophages (B).
Okukozesa Chonluten mu kuddaabiriza ebitundu by’omubiri eby’enjawulo
Okuddaabiriza ebitundu by’olususu: Mu mulimu gw’okuddaabiriza ebiwundu by’olususu, eddagala lya Chonluten eriziyiza okuzimba n’okutumbula okukula kw’obutoffaali kikulu nnyo. Ng’ekitundu ky’omubiri ekisinga obunene mu mubiri gw’omuntu, olususu lutera okufuna obuvune obw’enjawulo, gamba ng’okwokya n’okusala. Ebizimba bikola mangu oluvannyuma lw’okulumwa olususu, era okuzimba okuyitiridde kuyinza okulwawo okuwona ebiwundu n’okutuuka n’okuleeta enkovu. Chonluten asobola okuziyiza okufulumya ebintu ebizimba, okukendeeza ku kuddamu kw’okuzimba, ate mu kiseera kye kimu n’okutumbula obutoffaali bw’olususu, bwe kityo n’ayanguyiza okuwona kw’ebiwundu. Bw’ogeraageranya n’ebizigo by’ebiwundu eby’ekinnansi, ebisiba ebipya ebirimu Chonluten bisobola okutereeza obulungi embeera y’ebiwundu entonotono, okutumbula okuddamu okukola n’okuddaabiriza ebitundu by’olususu, ebiyinza okukendeeza ku kutondebwa kw’enkovu, n’okutumbula omutindo gw’okuddaabiriza olususu.
Ekifaananyi 3 Okukebera okunyweza obutoffaali–obutoffaali: Okugera obungi bwa confocal microscopy n’okwekenneenya ICAM-1 Western Blot.
Okuddaabiriza ebitundu by’amagumba: Mu nsonga y’okuddaabiriza ebitundu by’amagumba, wadde ng’okunoonyereza okuliwo kati tekukwata butereevu ku nkozesa ya Chonluten mu kuddaabiriza amagumba, peptides ezifaanagana eziddaabiriza ziraga ebisuubiza. Peptide B2A ekoleddwa esobola okuleetera obutoffaali obusibuka okwawukana ne bufuuka chondrocytes n’okutumbula okuddaabiriza kw’amagumba ayonoonese mu nvunza. Chonluten, n’obusobozi bwayo okulung’amya okukula kw’obutoffaali n’okuzimba, mu ndowooza nayo eyinza okuyamba mu kuddaabiriza amagumba. Oluvannyuma lw’okulumwa amagumba, okuzimba mu kitundu kubaawo, era Chonluten esobola okuziyiza okuzimba, okukendeeza ku kwonooneka kwayo eri chondrocytes. Okutumbula kwayo okukula kw’obutoffaali kuyinza okwanguyiza okukula n’okwawukana kw’obutoffaali obuyitibwa chondrocyte, okutumbula okusengejja kwa matrix y’amagumba, era bwe kityo ne kituuka ku kuddaabiriza ebitundu by’amagumba.
Ekifaananyi 4 Enkola ya B2A ku bulwadde bw’amagumba obuleetebwa MIA mu kinywa ky’okugulu kw’envunza.
Okuddaabiriza ebitundu by’amagumba: Mu nsonga y’okuddaabiriza ebitundu by’amagumba, okunoonyereza okuliwo kati okusinga kussa essira ku bika ebirala ebya peptides eziddaabiriza n’ebintu ebiramu. Okukozesa ekyuma ekikoleddwa mu PCL ekisiigiddwako peptides ezikoppa kolagini GFOGER kitumbula okutondebwa kw’amagumba mu bulema bw’amagumba ag’ekitundu eky’obunene bw’envunza; parathyroid hormone derivatives PTHrP-1 anchored to RADA16-I-formed self-assembled peptides P1R16 ziraga emirimu nga okutumbula okukula kw’obutoffaali obusibuka, okwawula okulung’amya, okuddamu okukola amagumba, n’okuzimba emisuwa, nga ziraga obusobozi bungi obw’okukola yinginiya w’ebitundu by’amagumba mu kuddaabiriza obulema oba okumenya amagumba. Ebikolwa bya Chonluten ebiziyiza okuzimba n’okutumbula okukula kw’obutoffaali bikendeeza ku kwonooneka kw’okuzimba kw’ebitundu by’amagumba mu biseera ebisooka eby’okuddaabiriza amagumba, bitumbula okukula kw’amagumba, era biteekawo omusingi gw’okuddamu okukola ebitundu by’amagumba.
Mu bufunzi
Mu bufunze, Chonluten, nga repair peptide, eraga ebikolwa ebinene mu kulungamya okukula kw’obutoffaali n’okuzimba. Enkola yaayo ey’okukola n’okunoonyereza ebizuuliddwa ku peptides endala ezifaanagana eziddaabiriza bikwata omugaso gw’okukozesa mu kitundu ky’okuddaabiriza ebitundu, omuli olususu, eggumba, n’amagumba.
Ebiwandiiko ebikozesebwa
[1] Avolio F, Martinotti S, Khavinson VK, n’abalala. Peptides ezifuga emirimu gy’okukula n’amakubo g’okuzimba mu Monocyte/Macrophage THP-1 Cell Line[J]. Ekitabo ky’ensi yonna ekya Sayansi wa Molekyulu, 2022,23 (7).DOI:10.3390/ijms23073607.
[2] Lin X, Shanmugasundaram S, Liu Y, n’abalala. B2A peptide ereeta enjawulo ya chondrogenic mu vitro era eyongera okuddaabiriza eggumba mu nvunza[J]. Journal of Okunoonyereza ku magumba, 2012,30 (8): 1221-1228.DOI: 10.1002 / jor.22078.
[3] Wojtowicz A. M., Shekaran A., Oest M. E., n’abalala. Okusiiga ebisenge by’ebiramu n’ekirungo kya kolagini ekikoppa peptide GFOGER okuddaabiriza obulema bw’amagumba[J]. Ebiramu, 2010, 31 (9): 2574-2582.DOI: 10.1016 / j.ebiramu.2009.12.008.
Ekintu ekiriwo okukozesebwa mu kunoonyereza kwokka:
