By Cocer Peptides 
Watanni 1 da suka gabata
DUKKANIN LABARI DA BAYANIN KYAUTATAWA DA AKE BAYAR A WANNAN SHAFIN KAWAI DOMIN WATSA BAYANI NE DA MANUFOFIN ILIMI.
Kayayyakin da aka bayar akan wannan gidan yanar gizon an yi niyya ne kawai don binciken in vitro. Binciken in vitro (Latin: *a cikin gilashi*, ma'ana a cikin gilashin gilashi) ana gudanar da shi a wajen jikin mutum. Waɗannan samfuran ba magunguna ba ne, Hukumar Abinci da Magunguna ta Amurka (FDA) ba ta amince da su ba, kuma dole ne a yi amfani da su don hanawa, magani, ko warkar da duk wani yanayi na likita, cuta, ko cuta. Doka ta haramta shigar da waɗannan samfuran cikin jikin mutum ko dabba ta kowace hanya.
peptides gyare-gyare sun nuna gagarumin tasiri a fagen gyaran nama da farfadowa. A matsayin peptide gyare-gyare, Chonluten yana taka rawa wajen daidaita ayyukan salula da inganta gyaran nama.
Hoto 1 Tsarin sinadaran Chonluten.
Matsayin Chonluten a cikin Yaduwan Tantanin halitta da Tsarin Kumburi
Ka'idar Yaduwa ta Tantanin halitta: Chonluten da jerin peptides da Farfesa Khavinson ya gano tun 1973 na iya daidaita maɓalli na yaduwa ta hanyar haɓaka tyrosine phosphorylation na kinases na furotin da ke kunna mitogen a cikin cytoplasm. Chonluten na iya rinjayar tsarin yaduwar kwayar halitta, inganta rarrabuwar tantanin halitta da girma, ta haka ne samar da tushe mai mahimmanci don gyaran nama. Misali, yayin warkar da rauni, yaduwar kwayar halitta muhimmin mataki ne a sabbin samuwar nama. Chonluten yana haɓaka wannan tsari ta hanyar daidaita ayyukan kinases masu alaƙa.
Tsarin Kumburi: Chonluten yana nuna ingantaccen tasiri a cikin tsarin kumburi. An samo shi daga ƙwayoyin epithelial na bronchial, yana hana samar da ƙwayar necrosis factor (TNF) ta hanyar monocytes da aka fallasa zuwa pro-inflammatory kwayoyin lipopolysaccharide (LPS) a cikin vitro. Monocytes suna taka muhimmiyar rawa a cikin martani mai kumburi; lokacin da LPS ta motsa su, suna sakin cytokines masu kumburi irin su TNF don fara kumburi. Chonluten yana rage ƙarfin amsawar kumburi ta hanyar hana samar da TNF. Duk peptides da aka gwada za su iya hana maganganun TNF da pro-inflammatory interleukin-6 (IL-6) cytokines a cikin LPS-stimulated ajali bambance-bambancen THP-1 Kwayoyin, kara nuna Chonluten ta tabbatacce rawa a daidaita kumburi microenvironment. A cikin martani mai kumburi wanda cututtuka masu kumburi ko lalacewar nama suka haifar, Chonluten na iya rage ƙarin lalacewar nama da ke haifar da kumburi ta wannan hanyar, samar da yanayi mai kyau don gyaran nama.
Hoto 2 Ƙididdigar ƙwayar ƙwayar ƙwayar cuta ta hanyar bincike na cytofluorimetric akan monocytes (A) da macrophages (B).
Aikace-aikacen Chonluten a cikin Gyaran Nama daban-daban
Gyaran Naman Fata: A fagen gyaran raunukan fata, Chonluten's anti-inflammatory and cell proliferating Properties na da muhimmiyar mahimmanci. A matsayinsa na mafi girma a cikin jikin mutum, fata yana da saukin kamuwa da raunuka daban-daban, kamar konewa da yankewa. Ana kunna martanin kumburi da sauri bayan rauni na fata, kuma yawan kumburi na iya jinkirta warkar da rauni har ma ya haifar da samuwar tabo. Chonluten na iya hana sakin abubuwan da ke haifar da kumburi, rage martani mai kumburi, kuma a lokaci guda yana haɓaka haɓakar ƙwayoyin fata, ta haka yana hanzarta warkar da rauni. Idan aka kwatanta da rigunan rauni na gargajiya, riguna na zamani wanda ke ɗauke da Chonluten zai iya daidaita microenvironment mai rauni, inganta haɓakar ƙwayar fata da gyare-gyare, yuwuwar rage samuwar tabo, da haɓaka ingancin gyaran fata.
Hoto 3 Cell-Cell Adhesion Assay: Ƙididdigar microscopy na Confocal da bincike na ICAM-1 Western Blot.
Gyaran nama na guringuntsi: Dangane da gyaran gyare-gyare na guringuntsi, kodayake bincike na yanzu bai magance aikace-aikacen Chonluten kai tsaye ba a cikin gyaran guringuntsi, irin wannan peptides gyare-gyare sun nuna sakamako mai ban sha'awa. peptide B2A na roba na iya haifar da sel mai tushe don bambanta cikin chondrocytes da haɓaka gyaran guringuntsi da aka lalace a cikin berayen. Chonluten, tare da ikonsa na daidaita yaduwar kwayar halitta da kumburi, a ka'idar kuma zai iya taimakawa wajen gyara guringuntsi. Bayan raunin guringuntsi, kumburi na gida yana faruwa, kuma Chonluten na iya kashe kumburi, rage lalacewarsa ga chondrocytes. Its promotion of cell proliferation may facilitate chondrocyte proliferation and differentiation, promote the synthesis of cartilage matrix, and thereby achieve cartilage tissue repair.
Hoto 4 Tasirin B2A akan Osteoarthritis mai haifar da MIA a cikin haɗin gwiwa na berayen.
Gyaran nama na kasusuwa: A fagen gyaran nama na kashi, bincike na yanzu ya fi mayar da hankali kan wasu nau'ikan gyaran peptides da biomaterials. Yin amfani da ɓangarorin roba na PCL wanda aka lulluɓe da collagen-mimicking peptides GFOGER yana haɓaka haɓakar ƙashi a cikin lahani mai girman girman bera; parathyroid hormone derivatives PTHrP-1 anchored to RADA16-I-kafa kai-taro peptides P1R16 nuna ayyuka kamar inganta kara cell yaduwa, directed bambance-bambancen, kashi gyare-gyaren, da kuma vascularization, nuna babban m ga kasusuwa nama injiniya a gyara kashi lahani ko karaya. Chonluten's anti-inflammatory and cell proliferation-proliferating effects rage kumburi lalacewa ga kasusuwa nama a farkon matakai na gyaran kashi, inganta yaduwar osteoblast, da kuma kafa harsashin farfadowa na nama na kashi.
Kammalawa
A taƙaice, Chonluten, a matsayin peptide na gyare-gyare, yana nuna tasiri mai mahimmanci wajen daidaita yaduwar kwayar halitta da kumburi. Hanyar aikinta da binciken bincike akan sauran peptides gyare-gyare irin wannan suna riƙe ƙimar aikace-aikacen a fagen gyaran nama, ciki har da fata, guringuntsi, da kashi.
Nassoshi
[1] Avolio F, Martinotti S, Khavinson VK, et al. Peptides Yana Gudanar da Ayyukan Yaduwa da Hanyoyi masu kumburi a cikin Monocyte/Macrophage THP-1 Cell Line[J]. Jarida ta Duniya na Kimiyyar Kwayoyin Halitta, 2022,23(7).DOI:10.3390/ijms23073607.
[2] Lin X, Shanmugasundaram S, Liu Y, et al. B2A peptide yana haifar da bambancin chondrogenic a cikin vitro kuma yana inganta gyaran guringuntsi a cikin berayen [J]. Jaridar Nazarin Orthopedic, 2012,30 (8): 1221-1228.DOI: 10.1002 / jor.22078.
[3] Wojtowicz AM, Shekaran A, Oest ME, et al. Rubutun ɓangarorin halittu tare da collagen-mimetic peptide GFOGER don gyara lahani na kashi[J]. Biomaterials, 2010,31 (9):2574-2582.DOI:10.1016/j.biomaterials.2009.12.008.
Samfura akwai don amfani da bincike kawai:
