IGF-1 LR3 1 mg

▎ IGF-1 LR3 Structure

▎ IGF-1 LR3 Kev Tshawb Fawb

Kev tshawb fawb keeb kwm yav dhau ntawm IGF-1 LR3 yog dab tsi?

Kev tshawb fawb ntawm IGF-1 LR3 pib los ntawm qhov xav tau kom kov yeej cov kev txwv ntawm cov tshuaj insulin zoo li kev loj hlob 1 (IGF-1). Raws li tus tswj hwm tseem ceeb ntawm kev loj hlob ntawm tes, sib txawv, thiab cov metabolism, ntuj IGF-1 qhia tau hais tias muaj peev xwm kho cov ntaub so ntswg, kev loj hlob thiab kev loj hlob, thiab kev cuam tshuam cov kab mob. Txawm li cas los xij, nws muaj qhov tsis zoo tseem ceeb: Nws ib nrab-lub neej tsuas yog ob peb teev, ua rau kev tshem tawm sai hauv vivo. Tsis tas li ntawd, nws nruj nreem khi rau IGF-binding proteins, uas ua rau ib tug tsawg feem ntawm dawb, active cov ntaub ntawv uas tawm tsam kom muaj kev cuam tshuam. Cov kev txwv no tau txwv nws txoj kev ua tau zoo hauv kev sim kev tshawb fawb thiab kev siv peev xwm, tsav cov kws tshawb fawb los tshawb nrhiav cov qauv hloov kho analogues nrog kev ua tau zoo.

Nrog rau kev nce qib hauv molecular biology thiab protein engineering thev naus laus zis, pab pawg tshawb fawb tau tsom mus rau kev hloov kho IGF-1 los txhim kho nws cov khoom. Los ntawm kev tshawb fawb tob ntawm cov qauv kev ua haujlwm ntawm IGF-1, cov kws tshawb nrhiav pom tias kev hloov kho ntawm cov chaw tshwj xeeb amino acid tuaj yeem cuam tshuam nws cov kev cuam tshuam nrog kev khi cov proteins thiab cov receptors: Hloov glutamic acid ntawm txoj hauj lwm 3 nrog arginine thiab ntxiv 13 amino acids rau N-terminus txo nws cov affinity rau active binding proteins thaum lub sij hawm binding. khi rau IGF-1 receptor. Nyob rau tib lub sijhawm, kev txhim kho kev ua kom zoo tau txuas ntxiv cov molecule lub voj voog metabolic hauv vivo, thaum kawg yielding IGF-1 LR3 nrog 83 amino acids. Qhov no ua rau ib nrab-lub neej ntev mus rau 20-30 teev thiab kwv yees li peb npaug ntxiv zog.

Yuav ua li cas yog lub mechanism ntawm kev txiav txim rau IGF-1 LR3?

Cell Proliferation thiab Differentiation

Stimulation ntawm Myoblast Proliferation: Thaum lub sij hawm fetal txoj kev loj hlob, IGF-1 LR3 ho txhawb kev loj hlob ntawm skeletal leeg myoblasts. Piv txwv li, kev tshawb fawb txog kev xeeb tub menyuam yaj fetuses tau qhia tias tom qab ib lub lim tiam ntawm IGF-1 LR3 infusion, skeletal leeg myoblast proliferation tus nqi nce (P <0.05). Qhov no qhia tau hais tias IGF-1 LR3 ncaj qha ua haujlwm ntawm myoblasts txhawm rau ua kom lub voj voog ntawm tes, ua kom muaj ntau lub myoblasts nkag mus rau hauv lub xeev proliferative thiab yog li muab cov cellular ntxiv rau cov leeg nqaij thiab kev loj hlob ^[1].

Influencing Follicular Development: Thaum lub sij hawm ntawm zes qe menyuam physiology, IGF-1 LR3 koom nrog tswj follicular kev loj hlob thiab kev loj hlob. Hauv cov qauv nas superovulation, kev sib koom ua ke ntawm IGF-1 LR3 nrog gonadotropins ntxiv nce ovulation tus nqi thiab nce qhov hnyav ntawm zes qe menyuam hauv qee hom nas. Qhov no qhia tias IGF-1 LR3 tuaj yeem cuam tshuam follicular maturation thiab ovulation los ntawm kev tswj hwm granulosa cell proliferation thiab sib txawv, yog li cuam tshuam rau kev ua me nyuam ^[2].

Kev cai ntawm Metabolism

Kev Tau Txais Kev Noj Qab Haus Huv thiab Kev Siv: Ua raws li IGF-1 LR3 infusion nyob rau hauv lub cev xeeb tub menyuam yaj fetuses lig, txo qis qaum amino acid uptake tus nqi tau soj ntsuam nrog rau qis dua fetal amino acid concentrations, txawm tias zoo sib xws fetal protein turnover tus nqi. Qhov no qhia tau hais tias IGF-1 LR3 tuaj yeem cuam tshuam rau tus menyuam hauv plab kev noj qab haus huv thiab kev siv cov qauv, ua kom muaj txiaj ntsig zoo ntawm kev siv cov khoom noj tsawg dua los pab txhawb kev loj hlob ntawm lub cev es tsis yog ua kom cov khoom noj hauv plab hauv plab los ntawm cov ntshav ntws los yog hloov cov khoom noj khoom haus ^[2].

Daim duab 1 IGF-1 inhibits o thiab accelerates angiogenesis ntawm Ras / PI3K / IKK / NF-κB qhia txoj hauv kev los txhawb qhov txhab kho ^[3].

Inhibition of Insulin Secretion: Infusion of IGF-1 LR3 los yog saline rau hauv cev xeeb tub thaum cev xeeb tub menyuam yaj tau qhia tias txo cov ntshav plasma insulin ntau hauv IGF-1 LR3-infused yaj. Tsis tas li ntawd, cov tshuaj insulin secretion thaum lub sij hawm kev sim hyperglycemic clamp tau txo qis piv rau kev tswj hwm. Qhov no qhia tias IGF-1 LR3 tuaj yeem ua ncaj qha rau ntawm pancreatic β-hlwb kom inhibit insulin secretion. Cov kev tshawb fawb ntxiv ntawm cov islets cais tawm tau qhia tias cov islets los ntawm IGF-1 LR3-infused lambs tau nthuav tawm cov tshuaj insulin tsis tu ncua nyob rau hauv cov lus teb rau hauv vitro qabzib stimulation, qhia tias IGF-1 LR3 tuaj yeem ua rau muaj qhov tsis xws luag hauv pancreatic β-hlwb, ua rau muaj qhov tsis zoo ntawm insulin secretion ^[4].

Txoj cai Angiogenesis

Lub luag haujlwm hauv zes qe menyuam angiogenesis: Hauv bovine luteinized follicle angiogenesis kab lis kev cai thwmsim, cov teebmeem ntawm IGF-1 LR3 ntawm luteinized follicle endothelial cell (EC) tes hauj lwm thiab progesterone ntau lawm tau tshawb xyuas. Cov txiaj ntsig tau pom tias muaj kev cuam tshuam tsawg rau EC kev loj hlob tsis tau tab sis nce me ntsis hauv kev loj hlob ntawm tes (3-5%). Hloov pauv, IGF-1 LR3 tau ua rau muaj kev cuam tshuam sib txawv ntawm kev tsim cov tshuaj progesterone, thaum IGF-1 receptor antagonist picropodophyllin (PPP) txo qis ob qho kev loj hlob ntawm EC thiab progesterone concentrations. Qhov no qhia tias IGF-1 LR3 tuaj yeem hloov kho vascularization thiab progesterone ntau lawm hauv luteinized follicles los ntawm IGF-1 receptor signaling pathway, yog li tswj xyuas zes qe menyuam thiab fertility ^[6].

Dab tsi yog daim ntawv thov ntawm IGF-1 LR3?

Tsiaj Loj hlob thiab Kev Txhim Kho Kev Tshawb Fawb thiab Kev Siv

Txhawb Fetal Organ Growth: Hauv kev sim nrog cov menyuam yaj thaum cev xeeb tub, kev tso dej ntawm IGF-1 LR3 ua rau muaj kev loj hlob ntawm fetal hauv nruab nrog cev, txhawb kev loj hlob ntawm cov kabmob xws li lub plawv, ob lub raum, spleen, thiab qog adrenal. Qhov no qhia tau hais tias IGF-1 LR3 plays lub luag haujlwm tseem ceeb hauv kev tswj cov txheej txheem kev loj hlob ntawm tus menyuam hauv plab, ua rau muaj kev nkag siab tob txog kev loj hlob ntawm tus menyuam hauv plab thiab kev loj hlob ntawm cov txheej txheem tswj hwm. Nws muab kev txhawb nqa theoretical thiab cov lus qhia ua haujlwm rau kev txhim kho tsiaj txhu kev loj hlob thiab txhim kho cov menyuam muaj sia nyob ^[1].

Stimulation ntawm Skeletal Muscle Myoblast Proliferation: Kev tshawb fawb qhia IGF-1 LR3 txhawb nqa pob txha pob txha myoblast proliferation. Hauv kev sim fetal yaj, IGF-1 LR3 infusion tau txhim kho myoblast proliferation kev ua ^[1].

Ntshav Qab Zib thiab Kev Tshawb Fawb Txog Kab Mob

Kev ntsuas qhov cuam tshuam ntawm Insulin secretion: Kev sim nrog IGF-1 LR3 infusion hauv fetal lambs qhia qhov txo qis ntawm fetal plasma insulin concentration. Hauv kev sim hyperglycemic clamp, qib insulin hauv IGF-1 LR3-kho fetal lambs yog 66% qis dua hauv kev tswj. Qhov tshwm sim no qhia txog kev sib koom ua ke ntawm IGF-1 LR3 thiab kev tso tawm insulin, muab cov ntsiab lus tseem ceeb rau kev kawm txog cov kab mob ntshav qab zib thiab tsim cov tswv yim kho tshiab ^[5].

Kev sib raug zoo nrog kev ua kis las: Kev tshawb fawb ntawm cov neeg tseem ceeb Israeli khiav thiab cov neeg ua luam dej tau qhia tias IGF1 noob polymorphisms cuam tshuam nrog IGF1 qib, thiab IGF genetic qhab nia (IGF-GS) ntawm sprinters yog txuam nrog kev ua kis las. Cov neeg tseem ceeb sprinters tau nthuav tawm cov qhab nia nruab nrab ntawm IGF-GS ntau dua li cov sprinters hauv teb chaws thiab cov neeg ua luam dej hauv theem siab. Qhov no qhia tias IGF-1 system tuaj yeem ua lub luag haujlwm tseem ceeb hauv kev ua si hauv av. Thaum nws tseem tsis tau paub meej tias IGF-GS tuaj yeem siv rau kev xaiv ntxov ntawm cov neeg tseem ceeb sprinters, nws muaj txoj hauv kev tshiab rau kev xaiv kis las thiab kev cob qhia. Kev tshawb fawb yav tom ntej tuaj yeem pab txhim kho cov phiaj xwm kev cob qhia ntau ntxiv los txhim kho kev ua kis las los ntawm kev saib xyuas thiab tshuaj xyuas IGF-1-txog cov cim hauv cov neeg ncaws pob ^[7].

Kev tshawb fawb hauv Cell Biology thiab Basic Medicine

Cell Proliferation Regulation Research: IGF-1 LR3 muaj lub peev xwm los txhawb kev loj hlob ntawm tes. Hauv vitro kev sim ua kom pom nws qhov kev txhawb nqa zoo ntawm NIH 3T3 cell proliferation. Qhov no ua rau IGF-1 LR3 yog ib qho cuab yeej tseem ceeb rau kev tshawb xyuas cov txheej txheem ntawm kev loj hlob ntawm tes. Los ntawm kev soj ntsuam IGF-1 LR3 cov teebmeem ntawm kev loj hlob ntawm cov kab sib txawv ntawm tes, cov kws tshawb nrhiav tuaj yeem nkag siab txog cov txheej txheem lom neeg xws li kev tswj hwm lub voj voog ntawm tes thiab cov kev taw qhia, muab lub hauv paus theoretical rau kev kawm hauv oncology, tshuaj kho dua tshiab, thiab lwm yam ^[8].

Apoptosis thiab Protein Metabolism Kev Tshawb Fawb: Hauv kev tshawb fawb ntawm hydrogen peroxide-kho C2C12 hlwb, IGF-1 (suav nrog nws cov analog IGF-1 LR3) modulates cellular protein synthesis thiab degradation tshuav nyiaj li cas los ntawm upregulating Pax7, myogenic regulatory yam (MRFs), mTOR, thiab P70RF1x AF, thiab inhibiting muaj Muv. tswj qhov sib npaug ntawm cov protein synthesis thiab degradation. Qhov no ua rau muaj kev nkag siab tob ntxiv ntawm cellular ciaj sia taus thiab kev tuag mechanisms nyob rau hauv cov kev ntxhov siab, nrog rau cov kev tswj xyuas ntawm cov protein metabolism, muab lub hom phiaj tshiab thiab kev nkag siab rau kev kho mob ntawm cov kab mob ^[9].

Xaus

IGF-1 LR3, raws li cov khoom siv ntev-ua yeeb yam ntawm cov tshuaj insulin zoo li kev loj hlob zoo li 1, txhawb kev loj hlob ntawm lub cev hauv lub plawv thiab cov qog adrenal los ntawm kev ua kom muaj kev taw qhia xws li PI3K / Akt thiab MAPK. Nws nkoos skeletal leeg myoblast proliferation thiab protein synthesis, tswj cov metabolism, tswj skeletal nqaij noj qab haus huv, thiab pab rov qab los ntawm kev tawm dag zog vim raug mob.

Hais Txog Tus Sau

Cov ntaub ntawv hais saum toj no yog txhua yam tshawb fawb, kho thiab sau los ntawm Cocer Peptides.

Scientific Journal Author

Feng L yog tus kws tshawb fawb tsom mus rau kev tawm dag zog lub cev thiab kev noj qab haus huv ntawm lub plawv. Lawv txoj haujlwm kev kawm feem ntau yog tsom rau kev tshawb fawb txog kev tswj hwm ntawm cov kev tawm dag zog sib txawv ntawm kev ua haujlwm ntawm lub cev, tshwj xeeb tshaj yog kev sib cuam tshuam ntawm kev tawm dag zog thiab lub cev cov txheej txheem molecular ntsig txog cov leeg nqaij thiab cov kab mob plawv. Dr. Feng feem ntau tau txais kev sib txuas ntawm cov qauv kev tshawb fawb ua ntej thiab cov txheej txheem molecular biology los ua qhov kev tshawb fawb tob. Feng L tau teev nyob rau hauv kev siv ntawm cov ntaub ntawv pov thawj [9].

▎ Cov Lus Qhia Tseem Ceeb

[1] Stremming J, White A, Donthi A, et al. Yaj recombinant IGF-1 txhawb kev loj hlob ntawm lub cev tshwj xeeb hauv fetal yaj. Frontiers hauv Physiology 2022; 13: 954948.DOI: 10.3389/fphys.2022.954948.

[2] Khamsi F, Roberge S, Wong J. Novel ua qauv qhia ntawm lub physiologic/pharmacologic luag hauj lwm ntawm insulin-zoo li kev loj hlob factor-1 nyob rau hauv ovulation nyob rau hauv nas thiab ua rau cumulus oophorus. Endocrine 2001; 14(2): 175-180.DOI: 10.1385/ENDO: 14:2:175.

[3] Zhang X, Hu F, Li J, et al. IGF-1 inhibits o thiab accelerates angiogenesis ntawm Ras / PI3K / IKK / NF-κB qhia txoj hauv kev los txhawb kev kho qhov txhab. European Journal of Pharmaceutical Sciences 2024; 200: 106847.DOI: 10.1016/j.ejps.2024.106847.

[4] White A, Stremming J, Boehmer BH, et al. Txo cov piam thaj-stimulated insulin secretion tom qab 1-wk IGF-1 infusion nyob rau hauv lig gestation fetal yaj yog vim muaj ib tug intrinsic islet defect. American Journal of Physiology-Endocrinology thiab Metabolism 2021; 320(6): E1138-E1147. DOI: 10.1152/ajpendo.00623.2020.

[5] White A, Stremming J, Brown LD, Rozance PJ. Attenuated qabzib-stimulated insulin secretion thaum lub sij hawm mob IGF-1 LR3 infusion rau hauv fetal yaj yaj tsis nyob rau hauv cov islets cais. Phau ntawv Journal of Developmental Origins of Health and Disease 2023; 14(3): 353-361.DOI: 10.1017/S2040 17442300009 0.

[6] Nwachukwu CU, Robinson RS, Woad KJ. Cov txiaj ntsig ntawm kev loj hlob zoo li insulin zoo li ntawm luteinising angiogenesis. Kev yug me nyuam thiab Fertility 2023; 4(2).DOI: 10.1530/RAF-22-0057.

[7] Ben-Zaken S, Meckel Y, Nemet D, Eliakim A. Insulin-like Growth Factor Axis Genetic Score thiab Sports Excellence. Phau ntawv Journal of Strength and Conditioning Research 2021; 35(9): 2421-2426.DOI: 10.1519/JSC.0000000000004102.

[8] Mao W. High-theem qhia ntawm cov saw ntev Arg~3-IGF-1 hauv Pichia pastoris thiab nws purification thiab characterization. Tsab ntawv xov xwm no ntawm Academy of Military Medical Sciences 2008. https://api.semanticscholar.org/CorpusID:88212024.

[9] Feng L, Li B, Xi Y, Cai M, Tian Z. Aerobic kev tawm dag zog thiab ua haujlwm tawm dag zog ua kom cov leeg pob txha atrophy los ntawm IGF-1 / IGF-1R-PI3K / Akt txoj hauv kev hauv nas nrog myocardial infarction. American Journal of Physiology-Cell Physiology 2022; 322(2): C164-C176.DOI: 10.1152/ajpcell.00344.2021.

Tag nrho cov kab lus thiab cov ntaub ntawv ntawm cov khoom lag luam muab rau ntawm lub vev xaib no tsuas yog rau cov ntaub ntawv tshaj tawm kev tshaj tawm thiab cov hom phiaj kev kawm.  

Cov khoom muab rau hauv lub vev xaib no yog npaj tshwj xeeb rau kev tshawb fawb hauv vitro. Kev tshawb fawb hauv vitro (Latin: * hauv iav *, lub ntsiab lus hauv iav) yog ua los ntawm tib neeg lub cev. Cov khoom no tsis yog tshuaj, tsis tau txais kev pom zoo los ntawm US Food and Drug Administration (FDA), thiab yuav tsum tsis txhob siv los tiv thaiv, kho, lossis kho txhua yam mob, kab mob, lossis mob. Nws raug txwv nruj raws li txoj cai los qhia cov khoom no rau hauv tib neeg lossis tsiaj lub cev hauv txhua daim ntawv.