IGF-1 LR3 1 mg

▎ IGF-1 LR3 Ọdịdị

▎ IGF-1 LR3 Nchọpụta

Kedu ihe ndabere nyocha nke IGF-1 LR3?

Nnyocha na IGF-1 LR3 sitere na mkpa ọ dị imeri njedebe nke ihe na-eto eto insulin-dị ka 1 (IGF-1). Dị ka isi ihe na-achịkwa uto cell, dị iche iche, na metabolism, IGF-1 eke na-egosi ike imezi anụ ahụ, ọganihu na ọmụmụ ihe mmepe, na ntinye ọrịa. Agbanyeghị, ọ nwere nnukwu ndọghachi azụ: ọkara ndụ ya na-ewe naanị awa ole na ole, na-eduga na mkpochapụ ngwa ngwa na vivo. Ọzọkwa, ọ na-ejikọta ya na protein ndị na-ejikọta IGF, na-ebute ọnụ ọgụgụ dị ala nke ụdị efu, na-arụ ọrụ nke na-agbasi mbọ ike inwe mmetụta na-adịgide adịgide. Mgbochi ndị a kpachibidoro ịdị irè ya na nyocha nnwale yana ngwa ndị nwere ike ime, na-eme ka ndị ọkà mmụta sayensị na-enyocha analogues emezigharịrị ahazi na arụmarụ ọrụ.

Site n'ọganihu na usoro ihe omimi ihe omimi na teknụzụ injinia protein, ndị otu nyocha lekwasịrị anya na imezi IGF-1 nke ọma iji melite akụrụngwa ya. Site na nyocha miri emi nke njikọ-arụ ọrụ nke IGF-1, ndị nchọpụta chọpụtara na mgbanwe na saịtị amino acid dị iche iche nwere ike imetụta mmekọrịta ya na protein na ndị na-anabata ya: Dochie acid glutamic na ọnọdụ 3 na arginine na-agbakwunye amino acid 13 na N-terminus na-ebelata njikọ ya maka IGF-njikọ protein na-adịghị arụ ọrụ, na-ebelata 1. onye nnata. N'otu oge ahụ, njikarịcha nhazi ahụ gbatịpụrụ usoro metabolic nke molekul na vivo, n'ikpeazụ na-enye IGF-1 LR3 nwere amino acid 83. Nke a mere ka ọkara ndụ dị ogologo ruo awa 20-30 na ihe dị ka okpukpu atọ mụbara ike.

Gịnị bụ usoro nke edinam maka IGF-1 LR3?

Mmụba cell na ọdịiche

Mkpali nke Myoblast Proliferation: N'oge mmepe nwa ebu n'afọ, IGF-1 LR3 na-akwalite mmụba nke myoblast muscle skeletal. Dịka ọmụmaatụ, ọmụmụ banyere nwa ebu n'afọ n'oge ime na-egosi na mgbe otu izu nke IGF-1 LR3 infusion, skeletal muscle myoblast proliferation rate abawanyela (P <0.05). Nke a na-egosi IGF-1 LR3 na-arụ ọrụ ozugbo na myoblasts iji kwalite usoro cell, na-eme ka ndị ọzọ myoblasts banye na steeti proliferative ma si otú ahụ na-enye ndị ọzọ cellular isi mmalite maka muscle anụ ahụ na mmepe ^[1].

Na-emetụta mmepe Follicular: N'oge physiology ovarian, IGF-1 LR3 na-ekere òkè n'ịhazi uto na mmepe follicular. N'ụdị superovulation oke oke, nkwado nke IGF-1 LR3 na gonadotropins na-abawanye ọnụego nwa nwa na ibuli ibu ovarian na ụfọdụ ụdị oke oke. Nke a na-egosi na IGF-1 LR3 nwere ike imetụta follicular maturation na ovulation site n'ịchịkwa granulosa cell proliferation na iche, si otú na-emetụta ọmụmụ ọrụ ^[2].

Usoro nke Metabolism

Nnweta na itinye n'ọrụ na-edozi ahụ: N'ịgbaso ntinye IGF-1 LR3 n'ime nwa ebu n'afọ ime n'oge ime, a na-ahụta ọnụ ọgụgụ nnabata amino acid dị ntakịrị n'akụkụ ọnụ ọgụgụ amino acid dị ala nke nwa ebu n'afọ, n'agbanyeghị ọnụego mgbanwe protein nwa ebu n'afọ. Nke a na-egosi na IGF-1 LR3 nwere ike imetụta oriri nwa ebu n'afọ na usoro iji ya eme ihe, na-eme ka ojiji nke ọma na-edozi ahụ nke ọma iji kwado uto nke akụkụ ahụ kama ịba ụba nke ihe oriri nwa ebu n'afọ site na ntinye ọbara placental ma ọ bụ mkpali nyefe nri ^[2].

Ọgụgụ 1 IGF-1 na-egbochi mbufụt ma na-eme ka angiogenesis dịkwuo elu site na ụzọ akara Ras / PI3K / IKK / NF-κB iji kwalite ọgwụgwọ ọnya ^[3].

Mgbochi nke nzuzo insulin: Ntinye nke IGF-1 LR3 ma ọ bụ saline n'ime ụmụ ebu n'afọ nke ime ime n'oge ekpughere mbelata mkpokọta insulin plasma na ụmụ atụrụ etinyere IGF-1 LR3. Na mgbakwunye, mmepụta insulin n'oge nnwale mwekota hyperglycemic belatara ma e jiri ya tụnyere njikwa. Nke a na-egosi na IGF-1 LR3 nwere ike ime ihe ozugbo na mkpụrụ ndụ β-pancreatic iji gbochie nzuzo insulin. Nnyocha ndị ọzọ na agwaetiti nwa ebu n'afọ dịpụrụ adịpụ kpughere na agwaetiti sitere na ụmụ atụrụ IGF-1 LR3 na-egosiputa insulin nzuzo na-adịgide adịgide na nzaghachi na mkpali glucose na vitro, na-atụ aro IGF-1 LR3 nwere ike ịkpata ntụpọ dị n'ime β-cell pancreatic, na-emebi ọrụ nzuzo insulin nkịtị ^[4,5]..

Usoro nke Angiogenesis

Ọrụ na ovarian angiogenesis: Na bovine luteinized follicle angiogenesis omenala nnwale, a na-enyocha mmetụta nke IGF-1 LR3 na netwọk follicle endothelial cell (EC) luteinized na mmepụta progesterone. Nsonaazụ gosiri obere mmetụta na oke uto EC mana mmụba ntakịrị na mmụba cell (3–5%). N'aka nke ọzọ, IGF-1 LR3 rụpụtara mmetụta dị iche iche na mmepụta progesterone, ebe IGF-1 onye na-anabata antagonist picropodophyllin (PPP) belatara nke ukwuu ma oke uto EC na mkpokọta progesterone. Nke a na-egosi IGF-1 LR3 nwere ike gbanwee vascularization na progesterone mmepụta na luteinized follicles site na IGF-1 receptor ụzọ akara, si otú na-ejigide ovarian ọrụ na ọmụmụ ^[6]..

Gịnị bụ ngwa nke IGF-1 LR3?

Nnyocha na ngwa mmepe nke anụmanụ na mmepe

Na-akwalite uto akụkụ nke nwa ebu n'afọ: N'ime nyocha nke nwa ebu n'afọ ime n'oge ime, ntinye nke IGF-1 LR3 na-abawanye ụba ngwa ngwa nwa ebu n'afọ, na-akwalite mmepe nke akụkụ ahụ dị ka obi, akụrụ, splin, na adrenal glands. Nke a na-egosi IGF-1 LR3 na-arụ ọrụ dị oke mkpa n'ịhazi usoro mmepe akụkụ nwa ebu n'afọ, na-enye aka n'ịghọta omimi nke uto nwa ebu n'afọ na usoro nhazi mmepe. Ọ na-enye nkwado usoro mmụta na ntụzịaka bara uru maka imeziwanye arụmọrụ ọmụmụ anụmanụ yana ịkwalite ọnụego nlanarị ụmụ ^[1].

Mkpali nke Skeletal Muscle Myoblast Proliferation: Nnyocha na-egosi IGF-1 LR3 na-akpali skeletal muscle myoblast proliferation. N'ime nnwale atụrụ nwa ebu n'afọ, infusion IGF-1 LR3 mere ka ọrụ myoblast dịkwuo elu nke ọma ^[1].

Ọrịa shuga na nyocha ọrịa metụtara

Ntụle mmetụta na nzuzo insulin: Nnwale metụtara ntinye IGF-1 LR3 n'ime ụmụ atụrụ nwa ebu n'afọ gosiri mbelata mkpokọta insulin plasma nwa ebu n'afọ. N'oge nnwale mwekota hyperglycemic, ọkwa insulin n'ime ụmụ atụrụ IGF-1 LR3 a na-agwọ dị obere 66% karịa na njikwa. Ihe omume a na-egosi njikọ dị n'etiti IGF-1 LR3 na insulin secretion, na-enye ihe ngosi dị mkpa maka ịmụ banyere pathogenesis nke ọrịa shuga na ịmepụta usoro ọgwụgwọ ọhụrụ ^[5]..

Mmekọrịta na arụmọrụ egwuregwu: Nnyocha na ndị na-agba ọsọ Israel na ndị na-egwu mmiri na-egosi na IGF1 gene polymorphisms jikọtara ya na ọkwa IGF1 na-ekesa, yana na akara mkpụrụ ndụ IGF (IGF-GS) nke ndị na-agba ọsọ na-ejikọta ya na arụmọrụ egwuregwu. Ndị na-agba ọsọ Elite gosipụtara akara IGF-GS dị elu nke ukwuu karịa ndị na-agba ọsọ mba na ndị na-egwu mmiri dị mkpụmkpụ. Nke a na-egosi na usoro IGF-1 nwere ike ịrụ ọrụ dị mkpa na egwuregwu ọsọ ụwa. Ọ bụ ezie na ọ ka na-ejighị n'aka ma enwere ike iji IGF-GS mee ihe maka nhọrọ mmalite nke ndị sprinters, ọ na-enye ụzọ ọhụrụ maka nhọrọ ndị na-eme egwuregwu na ọzụzụ ọzụzụ. Nnyocha n'ọdịnihu nwere ike ime ka mmepe nke mmemme ọzụzụ ezubere iche iji kwalite arụmọrụ egwuregwu site na nlekota na nyochaa akara ndị metụtara IGF-1 na ndị egwuregwu ^[7].

Nnyocha na Cell Biology na ọgwụ bụ isi

Nnyocha Iwu Mgbasa Cell: IGF-1 LR3 nwere ikike ịkpalite mmụba cell. Nnwale in vitro na-egosi mkpali ya dị irè nke mmụba cell NIH 3T3. Nke a na-enye IGF-1 LR3 ngwá ọrụ bara uru maka nyocha usoro nhazi mgbasawanye cellular. Site na ilele mmetụta IGF-1 LR3 na mmụba n'ofe mkpụrụ ndụ dị iche iche, ndị na-eme nchọpụta nwere ike nweta nghọta n'ime usoro usoro ndu dị ka usoro okirikiri cell na ụzọ akara, na-enye ntọala usoro maka ọmụmụ ihe na oncology, ọgwụ na-emegharị ahụ, na mpaghara ndị metụtara ya ^[8].

Apoptosis na Protein Metabolism Research: N'ime nnyocha nke hydrogen peroxide-mere C2C12 cell, IGF-1 (gụnyere ya analog IGF-1 LR3) modulates cellular protein synthesis na deradation itule site upregulating Pax7, myogenic regulatory ihe (MRFs), mTOR, na P70S6K na-eme ka apoptosis na-ebelata, na-ebelata apoptosis. nguzozi n'etiti njikọ protein na mmebi. Nke a na-enye aka na nghọta miri emi nke ndụ cellular na usoro ọnwụ n'okpuru ọnọdụ nrụgide, yana netwọk nchịkwa nke protein metabolism, na-enye ebumnuche ọhụrụ na nghọta maka ọgwụgwọ ọrịa ndị metụtara ya ^[9]..

Mmechi

IGF-1 LR3, dị ka synthetic na-arụ ọrụ ogologo oge analog nke insulin-dị ka ihe na-eto eto 1, na-akwalite uto akụkụ ahụ kpọmkwem na obi nwa ebu n'afọ na gland adrenal site na ịgbalite ụzọ mgbaàmà dịka PI3K/Akt na MAPK. Ọ na-akpali skeletal muscle myoblast proliferation na protein njikọ, na-achịkwa metabolism, na-ejigide ahụ ike akwara, na-enyere aka mgbake site na mmega ahụ kpatara mmerụ ahụ.

Banyere Onye dere ya

Ihe niile a kpọtụrụ aha n'elu bụ Cocer Peptides na-enyocha, dezie ma chịkọta ya.

Onye ode akwụkwọ akụkọ sayensị

Feng L bụ onye na-eme nchọpụta na-elekwasị anya na ngalaba nke mmega ahụ physiology na ahụike obi. Ọrụ agụmakwụkwọ ha gbadoro ụkwụ n'ịchọgharị mmetụta nchịkwa nke ụdị mmega ahụ dị iche iche na ọrụ physiological, ọkachasị mmekọrịta dị n'etiti mmega ahụ na usoro molekụla ahụ metụtara ahụike akwara na mgbake ọrịa obi. Dr. Feng na-anabatakarị ngwakọta nke ụdị nyocha nke izizi na usoro ihe ọmụmụ ihe omimi iji mee ọmụmụ ihe omimi. Edepụtara Feng L na ntụaka nke ntụaka [9].

▎ Okwu ndị dị mkpa

[1] Stremming J, White A, Donthi A, et al. Recombinant atụrụ IGF-1 na-akwalite uto akụkụ ahụ kpọmkwem na atụrụ nwa ebu n'afọ. Oke na Physiology 2022; 13: 954948.DOI: 10.3389/fphys.2022.954948.

[2] Khamsi F, Roberge S, Wong J. Ngosipụta ọhụụ nke ọrụ physiologic/pharmacoloji nke insulin-dị ka ihe na-eto eto-1 na ovulation na oke na ime ihe na cumulus oophorus. Endocrine 2001; 14(2): 175-180.DOI: 10.1385/ENDO:14:2:175 .

[3] Zhang X, Hu F, Li J, et al. IGF-1 na-egbochi mbufụt ma na-eme ka angiogenesis dịkwuo elu site na ụzọ akara Ras / PI3K / IKK / NF-κB iji kwalite ọgwụgwọ ọnya. European Journal of Pharmaceutical Sciences 2024; 200: 106847.DOI: 10.1016/j.ejps.2024.106847.

[4] White A, Stremming J, Boehmer BH, et al. Mbelata insulin na-akpali akpali nke glucose na-eso ntinye 1-wk IGF-1 n'ime atụrụ ime ime n'oge ikpeazụ bụ n'ihi ntụpọ n'ime ime agwaetiti. Akwụkwọ akụkọ American nke Physiology-Endocrinology na Metabolism 2021; 320(6): E1138-E1147. DOI:10.1152/ajpendo.00623.2020.

[5] White A, Stremming J, Brown LD, Rozance PJ. Mkpokọta insulin na-akpali akpali nke glucose n'oge nnukwu ntinye IGF-1 LR3 n'ime atụrụ nwa ebu n'afọ anaghị adịgide n'agwaetiti ndị dịpụrụ adịpụ. Akwụkwọ akụkọ mmalite mmepe nke ahụike na ọrịa 2023; 14(3): 353-361.DOI: 10.1017/S2040 17442300009 0.

[6] Nwachukwu CU, Robinson RS, Woad KJ. Mmetụta nke sistemu ihe uto dị ka insulin na luteinising angiogenesis. Mmepụta na ọmụmụ 2023; 4(2).DOI: 10.1530/RAF-22-0057.

[7] Ben-Zaken S, Meckel Y, Nemet D, Eliakim A. Insulin dị ka Growth Factor Axis Genetic Score and Sports Excellence. Akwụkwọ akụkọ nke ike na nyocha ọnọdụ 2021; 35(9): 2421-2426.DOI: 10.1519/JSC.0000000000004102.

[8] Mao W. Ngosipụta dị elu nke ogologo yinye Arg ~ 3-IGF-1 na Pichia pastoris na ịdị ọcha na njirimara ya. Akwụkwọ akụkọ nke Academy of Military Medical Sciences 2008. https://api.semanticscholar.org/CorpusID:88212024.

[9] Feng L, Li B, Xi Y, Cai M, Tian Z. Aerobic mmega ahụ na mmegide mmega ahụ na-ebelata atrophy skeletal muscle site na IGF-1 / IGF-1R-PI3K / Akt ụzọ na ụmụ oke na myocardial infarction. Akwụkwọ akụkọ American nke Physiology-Cell Physiology 2022; 322(2): C164-C176.DOI: 10.1152/ajpcell.00344.2021.

Edemede na ozi ngwaahịa niile enyere na webụsaịtị a bụ naanị maka mgbasa ozi na ebumnuche mmụta.  

Ngwaahịa ndị enyere na webụsaịtị a bụ naanị maka nyocha in vitro. A na-eme nyocha in vitro (Latin: * na iko *, nke pụtara na ihe eji enyo) na mpụga ahụ mmadụ. Ngwaahịa ndị a abụghị ọgwụ, ndị US Food and Drug Administration (FDA) akwadoghị ya, ha agaghịkwa eji ya gbochie, gwọọ, ma ọ bụ gwọọ ọnọdụ ahụike ọ bụla, ọrịa, ma ọ bụ ọrịa. Iwu amachibidoro nke ọma iwebata ngwaahịa ndị a n'ime ahụ mmadụ ma ọ bụ anụmanụ n'ụdị ọ bụla.