IGF-1 LR3 1mg

Imiterere ya IGF-1 LR3

▎ IGF-1 LR3 Ubushakashatsi

Ni ubuhe bushakashatsi bwakozwe na IGF-1 LR3?

Ubushakashatsi kuri IGF-1 LR3 bwaturutse ku gukenera gutsinda imbogamizi ziterwa na insuline karemano yo gukura 1 (IGF-1). Nkumuyobozi wingenzi witerambere ryimikorere, gutandukanya, hamwe na metabolism, IGF-1 karemano yerekana ubushobozi mugusana ingirangingo, gukura no kwiga iterambere, hamwe no gutabara indwara. Ariko, ifite ibibi byingenzi: Igice cyacyo cyigihe cyamasaha make, biganisha kumurongo wihuse muri vivo. Ikigeretse kuri ibyo, ihuza cyane na poroteyine za IGF zihuza, bikavamo umubare muto wubusa, ibikorwa bikora birwanira gukora ingaruka zirambye. Izi mbogamizi zabujije cyane imikorere yazo mubushakashatsi bwubushakashatsi hamwe nibishobora gukoreshwa, bituma abahanga mu bya siyansi bashakisha ibigereranyo byahinduwe muburyo bunoze.

Hamwe niterambere ryibinyabuzima bya molekuline na tekinoroji yubuhanga, amatsinda yubushakashatsi yibanze ku guhindura neza IGF-1 kugirango atezimbere imitungo yayo. Binyuze mu bushakashatsi bwimbitse ku mibanire n’imikorere ya IGF-1, abashakashatsi bavumbuye ko guhindura ahantu hihariye aside aside amine bishobora kugira ingaruka ku mikoranire yayo na poroteyine zihuza hamwe na reseptor: Gusimbuza aside glutamic ku mwanya wa 3 na arginine no kongeramo aside amine 13 kuri N-terminus bigabanya isano ifitanye na poroteyine IGF-1. Icyarimwe, uburyo bwiza bwo kwubaka bwongereye molekile ya metabolike ya vivo, amaherezo itanga IGF-1 LR3 hamwe na acide 83 amine. Ibi byaviriyemo igice cyubuzima bwongerewe amasaha 20-30 kandi imbaraga zigera kuri eshatu ziyongera.

Nubuhe buryo bwo gukora kuri IGF-1 LR3?

Gukwirakwiza Akagari no Gutandukana

Gukangura Myoblast Gukwirakwiza: Mugihe cyo gukura kw'inda, IGF-1 LR3 iteza imbere cyane ikwirakwizwa ry'imitsi myoblast. Kurugero, ubushakashatsi bwakozwe ku mwana wintama utinze-gesta bwerekanye ko nyuma yicyumweru kimwe cyo kwinjiza IGF-1 LR3, igipimo cyimitsi ya myoblast ikwirakwizwa cyane (P <0.05). Ibi byerekana ko IGF-1 LR3 ikora kuri myoblast kugirango iteze imbere ingirabuzimafatizo, ituma myoblast nyinshi yinjira mumyororokere bityo bigatanga andi masoko ya selile yo gukura kw'imitsi no gukura ^[1].

Kugira uruhare mu iterambere rya Follicular: Mugihe cya ovarian physiology, IGF-1 LR3 igira uruhare mukugenzura imikurire niterambere. Muburyo bwimbeba yimbeba, gufatanya kuyobora IGF-1 LR3 hamwe na gonadotropine byongereye umuvuduko wintanga ngore kandi byongera uburemere bwintanga ngore. Ibi byerekana ko IGF-1 LR3 ishobora kugira uruhare mu gukura no gukura kwa ovulation muguhuza ikwirakwizwa rya selile granulosa no gutandukana, bityo bikagira ingaruka kumikorere yimyororokere ^[2].

Amabwiriza ya Metabolism

Kubona intungamubiri no kuyikoresha: Nyuma yo kwinjizwa kwa IGF-1 LR3 mu nda ya nyuma yo gutwita, kugabanuka k'umugozi wa aminide aside aside byagaragaye hamwe no kugabanuka kwa aside amine acide, nubwo ibipimo bya poroteyine bisa. Ibi byerekana ko IGF-1 LR3 ishobora kugira uruhare mu gufata intungamubiri z’inda no mu buryo bwo kuyikoresha, bigafasha gukoresha neza intungamubiri nke kugira ngo zifashe imikurire yihariye aho kongera intungamubiri z’inda ziva mu maraso y’amaraso cyangwa gutera intungamubiri ^[2].

Igishushanyo 1 IGF-1 ibuza gucana kandi yihutisha angiogeneze ikoresheje Ras / PI3K / IKK / NF-κB inzira yerekana inzira yo gukiza ibikomere ^[3].

Kubuza gusohora kwa insuline: Kwinjiza IGF-1 LR3 cyangwa saline mu mwana w'intama utwite utinze gusama byagaragaje ko plasma insuline yagabanutse mu ntama za IGF-1 LR3. Byongeye kandi, gusohora kwa insuline mugihe cya hyperglycemic clamp ubushakashatsi bwagabanutse ugereranije no kugenzura. Ibi birerekana ko IGF-1 LR3 ishobora gukora muburyo butaziguye pancreatic β-selile kugirango ibuze insuline. Ubushakashatsi bwakozwe ku kirwa cyitaruye bwerekanye ko ibirwa biva mu ntama zatewe na IGF-1 LR3 byerekanaga intanga ngabo za insuline nkeya mu rwego rwo gusubiza ibibazo bya vitro glucose, byerekana ko IGF-1 LR3 ishobora gutera inenge imbere mu ngirabuzimafatizo ya pancreatic β-selile, bikangiza imikorere isanzwe ya insuline ^[4,5].

Amabwiriza ya Angiogenez

Uruhare muri ovarian angiogenez: Mu bushakashatsi bwakozwe na bovine luteinized follicle angiogenezi, ubushakashatsi bwakozwe ku ngaruka za IGF-1 LR3 ku miyoboro ya luteinized follicle endothelial selile (EC) n’umusaruro wa progesterone. Ibisubizo byagaragaje ingaruka nke ku bipimo byo gukura kwa EC ariko kwiyongera gake mu gukwirakwiza selile (3-5%). Ku rundi ruhande, IGF-1 LR3 yagize ingaruka zitandukanye ku musaruro wa progesterone, mu gihe IGF-1 reseptor antagonist picropodophyllin (PPP) yagabanije cyane ibipimo byo gukura kwa EC hamwe na progesterone. Ibi byerekana ko IGF-1 LR3 ishobora guhindura vasculaire hamwe na progesterone mu musemburo wa luteinize binyuze mu nzira ya reseptor ya IGF-1 yerekana inzira, bityo igakomeza imikorere yintanga nuburumbuke ^[6].

Ni ubuhe buryo bukoreshwa na IGF-1 LR3?

Gukura kwinyamaswa niterambere ryubushakashatsi nubushakashatsi

Guteza Imbere Gukura kw'Ingingo: Mu bushakashatsi bwakorewe ku mwana w'intama utinze-gutwita, kwinjiza IGF-1 LR3 byongereye cyane imikurire y'ingingo z'umwana, biteza imbere iterambere ry'ingingo nk'umutima, impyiko, impyiko, na glande ya adrenal. Ibi byerekana ko IGF-1 LR3 igira uruhare runini muguhindura imikorere yiterambere ryimyanya myibarukiro, bigira uruhare mugusobanukirwa byimazeyo imikurire yimikorere nuburyo bwo kugenzura iterambere. Itanga ubufasha bufatika hamwe nubuyobozi bufatika bwo kunoza imikorere yimyororokere yinyamanswa no kuzamura imibereho yabana ^[1].

Gukangura Imitsi ya Skeletal Myoblast Ikwirakwizwa: Ubushakashatsi bwerekana ko IGF-1 LR3 itera imitsi ya skeletale myoblast ikwirakwizwa. Mu bushakashatsi bw'intama z'inda, IGF-1 LR3 kwinjiza byongereye cyane ibikorwa byo gukwirakwiza myoblast ^[1].

Diyabete n'ubushakashatsi bujyanye n'indwara

Gusuzuma Ingaruka ku Gusohora kwa Insuline: Ubushakashatsi bujyanye no kwinjiza IGF-1 LR3 mu mwana w'intama w'inda bwerekanye ko igabanuka rya plasma insuline. Mugihe cyibigeragezo bya hyperglycemic clamp, urugero rwa insuline muri IGF-1 LR3 yintama zavuwe zintama zari munsi ya 66% ugereranije no kugenzura. Iki kintu cyerekana isano iri hagati ya IGF-1 LR3 na insuline isohoka, itanga ibimenyetso byingenzi byo kwiga indwara ya diyabete no gushyiraho ingamba nshya zo kuvura ^[5].

Isano hamwe n’imikino ngororamubiri: Ubushakashatsi bwakozwe ku basiganwa bakomeye bo muri Isiraheli biruka ndetse no koga byerekanye ko IGF1 gene polymorphism ifitanye isano no kuzenguruka urwego rwa IGF1, kandi ko amanota ya IGF (IGF-GS) ya siporo ajyanye no kwitwara neza. Elite sprinters yerekanaga cyane amanota yo hejuru ya IGF-GS kurusha amanota yo murwego rwigihugu ndetse no koga murwego rwo hejuru. Ibi birerekana ko sisitemu ya IGF-1 ishobora kugira uruhare runini muri siporo yihuta kwisi. Nubwo bitazwi neza niba IGF-GS ishobora gukoreshwa muguhitamo hakiri kare intore zidasanzwe, itanga inzira nshya zo gutoranya abakinnyi no kwitabira imyitozo. Ubushakashatsi bw'ejo hazaza bushobora gutuma gahunda zamahugurwa zigamije kuzamura imikorere ya siporo mugukurikirana no gusesengura ibimenyetso bifitanye isano na IGF-1 mu bakinnyi ^[7].

Ubushakashatsi muri Biologiya selile nubuvuzi bwibanze

Ubushakashatsi bwo Gukwirakwiza Ingirabuzimafatizo Ubushakashatsi: IGF-1 LR3 ifite ubushobozi bwo gukangura ingirabuzimafatizo. Mubushakashatsi bwa vitro bwerekana imbaraga zayo zo gukwirakwiza selile ya NIH 3T3. Ibi bihindura IGF-1 LR3 igikoresho cyingenzi cyo gukora iperereza ryuburyo bwo gukwirakwiza selile. Mu kwitegereza ingaruka za IGF-1 LR3 ku ikwirakwizwa ry’imirongo itandukanye, abashakashatsi barashobora kungukirwa nubuzima bwibanze bwibinyabuzima nko kugenzura ingirabuzimafatizo no kwerekana inzira, bitanga umusingi w’amahame y’ubushakashatsi kuri onkologiya, ubuvuzi bushya, hamwe n’ibindi bifitanye isano ^[8].

Ubushakashatsi bwa Apoptose na Protein Metabolism: Mu bushakashatsi bwakozwe na selile ya hydrogène peroxide ivurwa na C2C12, IGF-1 (harimo na analogi yayo IGF-1 LR3) ihindura intungamubiri za poroteyine ngengabuzima hamwe no gutesha agaciro igenzura Pax7, ibintu bigenga myogenic (MRFs), mTOR, na P70S6K, bikagabanya uburinganire bwa MuRF1 na MAFbx, kandi bikabuza kwangiza kwa MuRF1 na MAFbx. Ibi bigira uruhare mu gusobanukirwa byimbitse uburyo bwo kubaho kwingirabuzimafatizo hamwe nuburyo bwo gupfa mu bihe bigoye, ndetse n’imiyoboro igenga metabolisme ya poroteyine, itanga intego nshya n’ubushishozi bwo kuvura indwara zifitanye isano ^[9].

Umwanzuro

IGF-1 F Itera imitsi ya skeletal myoblast ikwirakwizwa na proteyine ya proteyine, igenga metabolisme, ikomeza ubuzima bwimitsi ya skeletale, kandi igafasha gukira ibikomere biterwa na siporo.

Ibyerekeye Umwanditsi

Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.

Umwanditsi w'ikinyamakuru

Feng L ni umushakashatsi wibanze kubijyanye na siporo physiologie nubuzima bwumutima. Ibikorwa byabo byamasomo byibanda cyane cyane ku ngaruka ziterwa nuburyo butandukanye bwimyitozo ngororamubiri ku mikorere ya physiologiya, cyane cyane imikoranire hagati yimyitozo ngororamubiri hamwe na molekulike yumubiri ijyanye nubuzima bwimitsi no gukira indwara zifata umutima. Muganga Feng akunze gukoresha uburyo bwubushakashatsi bwibanze nubuhanga bwibinyabuzima bwa molekuline kugirango akore ubushakashatsi bwimbitse. Feng L yanditse kurutonde rwerekana [9].

Imirongo ijyanye

[1] Gukubita J, Umweru A, Donthi A, n'abandi. Intama recombinant IGF-1 ituma imikurire yihariye yintama zintama. Imipaka muri Physiology 2022; 13: 954948.DOI: 10.3389 / fphys.2022.954948.

2 Endocrine 2001; 14(2): 175-180.DOI: 10.1385 / ENDO: 14: 2: 175.

[3] Zhang X, Hu F, Li J, n'abandi. IGF-1 ibuza gucana kandi yihutisha angiogeneze ikoresheje Ras / PI3K / IKK / NF-κB inzira yerekana inzira yo gukira ibikomere. Ikinyamakuru cyo mu Burayi cy’ubumenyi bwa farumasi 2024; 200: 106847.DOI: 10.1016 / j.ejps.2024.106847.

[4] Umweru A, Gukubita J, Boehmer BH, n'abandi. Kugabanya glucose-iterwa na insuline isohoka nyuma yo kwinjiza 1-wk IGF-1 mugihe cyo gutwita intama zinda zitwite biterwa nubusembwa bwikirwa. Ikinyamakuru cyo muri Amerika cya Physiology-Endocrinology na Metabolism 2021; 320(6): E1138-E1147. DOI: 10.1152 / ajpendo.00623.2020.

[5] Umweru A, Gukubita J, Brown LD, Rozance PJ. Indwara ya glucose iterwa na insuline isohoka mugihe cya IGF-1 LR3 ikabije mu ntama z'inda ntiziguma mu birwa byitaruye. Ikinyamakuru cyiterambere ryiterambere ryubuzima nindwara 2023; 14(3): 353-361.DOI: 10.1017 / S2040 17442300009 0.

[6] Nwachukwu CU, Robinson RS, Woad KJ. Ingaruka ya insuline isa na sisitemu yo gukura kuri luteinising angiogenez. Imyororokere n'uburumbuke 2023; 4(2) .DOI: 10.1530 / RAF-22-0057.

[7] Ben-Zaken S, Meckel Y, Nemet D, Eliakim A. Insuline imeze nkikura ryikura rya Axis Amanota ya genetike na siporo nziza. Ikinyamakuru cyimbaraga nubushakashatsi 2021; 35(9): 2421-2426.DOI: 10.1519 / JSC.0000000000004102.

[8] Mao W. Imvugo yo murwego rwohejuru rwurunigi rurerure Arg ~ 3-IGF-1 muri pasitori ya Pichia no kwezwa no kuranga. Amatangazo y'Ishuri Rikuru ry'Ubuvuzi bwa Gisirikare 2008. https://api.semanticscholar.org/CorpusID:88212024.

[9] Feng L, Li B, Xi Y, Cai M, Tian Z. Imyitozo yo mu kirere no kurwanya imyitozo ngororamubiri igabanya imitsi ya skeletale binyuze muri IGF-1 / IGF-1R-PI3K / Akt inzira mu mbeba hamwe na infirasiyo ya myocardial. Ikinyamakuru cyo muri Amerika cya Physiology-Cell Physiology 2022; 322(2): C164-C176.DOI: 10.1152 / ajpcell.00344.2021.

INGINGO ZOSE N'AMAKURU Y’IBICURUZWA BITANZWE KURI URU RUBUGA BISANZWE KUBONA AMAKURU N'INTEGO Z'UBUREZI.  

Ibicuruzwa byatanzwe kururu rubuga bigenewe gusa mubushakashatsi bwa vitro. Mu bushakashatsi bwa vitro (Ikilatini: * mu kirahure *, bisobanura mu bikoresho by'ibirahure) bikorwa hanze y'umubiri w'umuntu. Ibicuruzwa ntabwo ari imiti, ntabwo byemejwe n’ikigo cy’Amerika gishinzwe ibiryo n’ibiyobyabwenge (FDA), kandi ntigomba gukoreshwa mu gukumira, kuvura, cyangwa gukiza indwara iyo ari yo yose y’ubuvuzi, indwara, cyangwa indwara. Birabujijwe rwose n'amategeko kwinjiza ibyo bicuruzwa mumubiri wabantu cyangwa inyamaswa muburyo ubwo aribwo bwose.