Ort Cort-g Ubushakashatsi
Ni ubuhe bushakashatsi bwakozwe na Cort-g?
Nka tetrapeptide ya syntetique, Cort-g ikomoka mubushakashatsi bwubushakashatsi mubice byinshi. Mu rwego rwa neuroscience, bitewe n’indwara zidakira zidakira nk’indwara ya Alzheimer n’indwara ya Parkinson, abashakashatsi bagiye bashakisha ibintu bishobora kurinda neuroni no guteza imbere imitsi. Cort-g yavukiye muri ubu bushakashatsi kandi biteganijwe ko izafasha mu gusana neuron no kugabanya umuvuduko w'indwara. Mu bushakashatsi bw’ubudahangarwa, bitewe n’uko indwara z’ubudahangarwa zitera indwara nyinshi, abahanga bagiye bakora ubushakashatsi kuri molekile nto kugira ngo bagabanye ubudahangarwa bw'umubiri.
Cort-g irashobora kugenzura sisitemu yumubiri, igatera imvugo ya interleukin-2, kandi ikagabanya ibisubizo bya autoimmune, ikazana ibitekerezo bishya byo kuvura indwara zumubiri. Hamwe n’indwara nyinshi zifata umutima nimiyoboro nubwonko bwubwonko, ingaruka za Cort-g ku bipimo bifatika no kwerekana imiterere ya gene yumutima bitanga icyerekezo gishya cyo gukumira no kuvura izo ndwara. Bitewe n’inyungu zigenda ziyongera mu bushakashatsi bwo kurwanya gusaza bitewe n’ubusaza bw’abaturage, Cort-g irashobora kugenga ingirabuzimafatizo no gutuma ADN yegera igihugu gito, bikagira uruhare mu guhishura uburyo bwo gusaza no kugera ku gusaza kwiza. Byongeye kandi, kubera ko gusana ingirabuzimafatizo no kuvugurura ari byo byingenzi byibandwaho mu buvuzi, ibiranga Cort-g mu guteza imbere ingirabuzimafatizo no gukangurira imitsi kuvugurura imitsi byafunguye inzira nshya y'ubushakashatsi muri uru rwego.
Nubuhe buryo bwo gukora bwa Cort-g?
1. Ingaruka kuri Heterochromatine
Gutera Depolymerisation ya Heterochromatine Yuzuye: Peptide bioregulator Cort-g irashobora gutera ubushake bwa deheterochromatisation (depolymerisation) ya heterochromatine yose. Through the use of molecular cytogenetic methods, such as differential scanning calorimetry, analyzing the activity of ribosomal genes in the satellite stalks of acrocentric chromosome NORs, studying the polymorphism of centromeric C-heterochromatin in the peripheral structure, and observing the variability of facultative heterochromatin (FH) in the lymphocytes cultured from individuals aged 80 and above, it has been found that Cort-g has a significant regulatory effect on heterochromatin [1].
Gukora Ribosomal Gene Synthesis: Bitewe no gukora deheterochromatine yibikorwa bya satelite ya chromosomes yegeranye, Cort-g ikora synthesis ya genes ya ribosomal. Muri icyo gihe, ntabwo itera deheterochromatisation ya heterochromatine igizwe na centromere, kandi ikarekura genes zabujijwe kubera guhuza uturere twa autosomal bigize FH [1].
2. Ingaruka kuri Neurons
Kugenga ibikorwa byamashanyarazi ya Neurons: Ibiyobyabwenge cortexine na peptide ya cortexine byagaragaye ko bigenga ibikorwa byamashanyarazi ya neurone muburyo bumwe, ni ukuvuga guhindura muburyo bushoboka ubushobozi bwo kuruhuka, ubushobozi bwibikorwa, hamwe ninshuro ya impulse, bisobanurwa nkingaruka zo gukora. Glycine irashobora kandi gukora ibikorwa bya neuron, ariko kurwego ruto. Tetrapeptide Cort-g irashobora kandi hyperpolarize neuron kuri mV 2-3 kandi ikagabanya ibikorwa byayo bidatinze kuri 0.1-100 μM, byerekana ingaruka zayo (neuroprotective). Ku gipimo cya 1000 μM, Cort-g igabanya urugero rwa neuron (2-4 mV), kandi kwiyongera mubikorwa bya impulse byanditswe. Ku gipimo cya mM 10, kigaragara cyane kandi gisubiza inyuma neuron, cyongera inshuro, kandi kibuza kubyara ubushobozi bwibikorwa [1].
Ingaruka ku miyoboro ya Ion: Cort-g irashobora kandi kongera amplitude yumuvuduko wimbere ugana 3-5% kuri concentration ya 0.1 μM. Nyuma yo gutanga Cort-g, nta kwiyongera kwa amplitude (activation) yimiyoboro ya sodium yimbere na calcium byagaragaye. Nyuma yo gutanga Cort-g kuri concentration ya 100 μM no hejuru yayo, amplitude yiyi miyoboro yatangiye kwerekana urugero rushingiye kandi rwinshi. Nyuma ya peptide yibanze kuri mM 10, kubuza bishobora kugera kuri 80-90%. Muri byo, kubuza imiyoboro ya sodium irakomeye kuruta iy'imiyoboro ya calcium [1].
3. Ingaruka kuri Cardiac Gene Yerekana
Guhindura Indwara ya Cardiac: Mu gusesengura inyandiko-mvugo 15,247 mu mitima y’imbeba z’umugore w’amezi 6 CBA zatewe inshinge zihoraho za Cort-g mu gihe cy’iminsi 5, byagaragaye ko ugereranije n’itsinda rishinzwe kugenzura, CDNA microarray ivanga ingero z'umutima mu itsinda ry’ubushakashatsi ryerekanye ko imvugo ya clone 234 (ihwanye na 1.53% ya clon ikora) yari ifite clon zose zamenyekanye kandi zifite ibice 110 bya clon izwi). Ntarengwa hejuru-kugenzura no kumanura byari +5.42 na -2.86 bikurikiranye [2].
Ugereranije nizindi Peptide na Hormone: Kugereranya impinduka mumwirondoro yumutima wumutima uterwa na peptide ya peptide (Cort-g, Vilon, Epitalon) hamwe na pineine peptide hormone melatonin igaragaza ingaruka rusange kandi zihariye za Cort-g kumagambo ya gene yumutima [2].
4. Uruhare Mubindi Bice
Ingaruka ku mitsi yangiritse ya Periferique: Mu bantu, Cort-g igira ingaruka zigaragara zo kuvura ku miterere n'imikorere yo gukira kw'imitsi yangirika ya peripheri. Ni peptide ya sintetike yabonetse binyuze muri synthesis yerekanwe hashingiwe ku isesengura rya aminide acide ya cerebral cortex peptide itegura Cortexin [1].
Uruhare muri Hip Osteoarthritis: Ubushakashatsi bumwe bwerekanye ko inshinge zo mu bwoko bwa corticosteroid zatewe mu myaka mirongo. Nka peptide bioregulator ifite ingaruka zisa, Cort-g igira uruhare murwego runaka muguhindura ibisubizo byumuriro nizindi nzira [3].
Ni ubuhe buryo bukoreshwa bwa Cort-g?
1. Kurwanya gusaza
Gutinda gusaza: Nka peptide irwanya gusaza, Cort-g irashobora kugenga imvugo ya gene, igasubiza ADN kumuto, bityo bikagabanya gusaza no kongera gukora genes runaka. Ubusaza busanzwe buherekezwa nimpinduka mumagambo ya gene. Cort-g irashobora gutinza gusaza muguhindura imvugo ya genes. Kurugero, irashobora kugera ku ngaruka zo kurwanya gusaza mugukora ibikorwa bya telomerase, kugabanya kwangirika kwa okiside, cyangwa kugenzura ihinduka rya epigenetike.
Kugenga Metabolism ya Cellular: Irashobora guhagarika imitekerereze ya metabolike mu bwonko iterwa na ischemia idakira, ifite inyungu zishobora kubungabunga ubuzima bwubwonko n'imikorere [2] . Indwara ya metabolike mu bwonko ifitanye isano n'indwara zitandukanye zifata ubwonko n'indwara ziterwa no gusaza. Cort-g irashobora kugarura imikorere isanzwe ya metabolike muguhindura inzira ya metabolike mubwonko, bityo bikarinda ubuzima bwubwonko.
1. Neuroprotection no Gusana Ubwonko
Guteza imbere imikorere ya Neuron: Cort-g irashobora gukangura imikorere nigikorwa cya neuron, bigira uruhare runini mugusubirana no kubungabunga imikorere ya neuron. Ubushakashatsi bwerekanye ko bushobora guteza imbere imikurire n’iterambere rya neurone mu guhuza n’imikurire y’imitsi [4] . Ubu buryo bwibikorwa bushobora kuba bukubiyemo inzira zerekana ibimenyetso byihariye, bityo bikazamura ibikorwa bya metabolike hamwe nogukwirakwiza synaptic ya neuron.
Gufasha mu kugarura imitsi ya Nervice: Cort-g yerekanye ubushobozi muburyo bwo gukira kwimikorere no mumikorere yimitsi yinyuma ya periferique, ifasha mugukosora ibikomere byimitsi. Mugutunganya ibice n'imiterere ya matrice idasanzwe, itanga ibidukikije bikwiye kugirango imikurire ya selile. Matrisa idasanzwe igira uruhare runini mukubungabunga selile homeostasis no gukura kwinyama. Cort-g irashobora guteza imbere kuvugurura no gusana ingirangingo zifata ibice bigize imikorere ya matrice idasanzwe.
Ubushakashatsi ku ndwara zifata ubwonko: Cort-g ni imwe mu ngingo zishyushye mu bushakashatsi bw’indwara zifata ubwonko nk'indwara ya Alzheimer n'indwara ya Parkinson. Ifasha kugabanya umuvuduko cyangwa gukumira izo ndwara. Indwara zifata ubwonko zisanzwe zifitanye isano nurupfu no kudakora neza kwa neuron. Cort-g irashobora kugabanya kwangirika no gupfa kwa neurone binyuze mu ngaruka zayo zifata ubwonko, bityo bikadindiza iterambere ry’indwara. Kurugero, irashobora kugira uruhare muguhuza urwego rwa neurotransmitter, kuzamura sisitemu yo kwirinda antioxydeant, cyangwa guteza imbere ikwirakwizwa no gutandukanya ingirabuzimafatizo.
2. Kongera ubumenyi
Gutezimbere Kwibuka no Kwitonda: Ubushakashatsi bwerekanye ko Cort-g ishobora kunoza kwibuka, kwitondera, hamwe nubushobozi bwubwenge, byongera imikorere yubwenge. Uburyo bwibikorwa byabwo bushobora kuba bukubiyemo kugenzura imikorere ya neurotransmitter mu bwonko, nko kongera irekurwa rya neurotransmitter nka dopamine na norepinephrine [5] . Izi neurotransmitter zigira uruhare runini mubikorwa byo kumenya. Kurugero, dopamine ifitanye isano na sisitemu yo guhemba no kwiga no kwibuka, na norepinephrine ifitanye isano no kwitonda no kuba maso.
Kugabanya Imyitwarire Ihangayitse: Cort-g igira ingaruka zo kurwanya amaganya kandi irashobora gufasha kugabanya imyitwarire ijyanye no guhangayika, bityo igatera amarangamutima. Mu bushakashatsi bwimbeba, Cort-g yerekanye ingaruka zigenga imyitwarire ijyanye no guhangayika [4] . Irashobora kugabanya ibimenyetso byo guhangayika bigira ingaruka kumitsi yubwonko, nko kugenzura ibikorwa byakarere nka amygdala na hippocampus.
3. Gukingira indwara
Gukangura imvugo ya Interleukin-2: Cort-g irashobora gukangura imvugo ya interleukin-2, bityo igenga imikorere yubudahangarwa no kongera ubudahangarwa bw'umubiri. Interleukin-2 ni ikintu cyingenzi cyo gukingira indwara gishobora guteza imbere ikwirakwizwa ry’imikorere ya T ndetse no kongera imikorere y’umubiri w’umubiri [1] . Cort-g irashobora gutuma imvugo ya interleukin-2 ikora inzira yerekana ibimenyetso, bityo igahindura ubudahangarwa bw'umubiri.
Kugabanya Ibisubizo bya Autoimmune: Ifasha kugenzura imikorere yumubiri kugabanya ibisubizo bya autoimmune kandi birashobora kugira ingaruka runaka kuburwayi bwa autoimmune. Indwara za Autoimmune ziterwa na sisitemu yumubiri yumubiri yibeshya yibasira ingirangingo zayo. Cort-g irashobora kugabanya ibibazo bya autoimmune ibisubizo muguhuza ibikorwa bya sisitemu yumubiri, bityo bikagira uruhare runini rwo kuvura indwara ziterwa na autoimmune.
4. Gusana imyenda no kuvugurura
Kwihutisha gukira ibikomere: Cort-g irashobora kwihutisha gukira nyuma yimvune, harimo gukomeretsa ingirangingo no gukira nyuma yo kubagwa, biteza imbere ingirabuzimafatizo. Matrice idasanzwe ifite ibyiza byihariye muguhindura no gusana. Cort-g irashobora guteza imbere ikwirakwizwa no gutandukanya ingirabuzimafatizo muguhindura ibice n'imikorere ya matrice idasanzwe, kwihutisha gusana no kuvugurura.
Guteza imbere imitsi mishya: Cort-g irashobora gutera imbaraga kuvugurura imitsi kandi ikagira ingaruka nziza mugusana no kuvugurura ingirabuzimafatizo za periferique. Kuvugurura imitsi ni inzira igoye irimo gukwirakwira, gutandukana, no kwimuka kwingirangingo ngengabuzima, kimwe no gukura kwa axon no gukora synaps. Cort-g irashobora guteza imbere imitekerereze mishya muguhuza inzira nyamukuru yerekana inzira muriki gikorwa.
5. Ubuzima bwumutima nimiyoboro yubwonko
Ingaruka ku bipimo byerekana umutima n'ubwonko: Ubushakashatsi bumwe bwerekanye ko Cort-g ishobora kugira ingaruka ku bipimo bimwe na bimwe byerekana sisitemu y'umutima n'imitsi, nubwo kariya gace kagikeneye ubundi bushakashatsi. Kurugero, irashobora kugira ingaruka kubipimo nkumuvuduko wamaraso, umuvuduko wumutima, hamwe nubworoherane bwimitsi (Anisimov S, 2004). Uburyo bwibikorwa bushobora kuba bukubiyemo kugenzura imiyoboro ya neurotransmitter, imisemburo, hamwe ninzira zerekana inzira mumitsi yumutima nimiyoboro yubwonko.
Kugena imiterere yumutima wa Cardiac: Cort-g irashobora cyane kugenzura-kugenzura imiterere ya genes zimwe na zimwe mumutima, nka Pass1, Hsc70, Bmp2, Wnt4, Eps15, na Eps15-rs. Izi ngirabuzima fatizo zifitanye isano no kubaho kw'uturemangingo tw'umutima, gukora ingirangingo z'umutima, no kugenzura imfu ziterwa na selile [2] . Mugutunganya imiterere yumutima wumutima, Cort-g irashobora kugira ingaruka nziza kumiterere nimikorere yumutima, bityo igafasha kubungabunga ubuzima bwumutima.
6. Amabwiriza agenga imyifatire
Gutezimbere Amarangamutima: Cort-g irashobora kugira ingaruka muguhindura imyumvire, kunoza amarangamutima, no kugabanya ibimenyetso byo kwiheba no guhangayika. Uburyo bwibikorwa byabwo bushobora kuba bujyanye no kugenzura imikorere ya neurotransmitter na sisitemu yo mu bwonko [4] . Kurugero, irashobora guteza imbere amarangamutima mukongera urwego rwa neurotransmitter nka serotonine na dopamine; cyangwa irashobora kugabanya ibimenyetso byo guhangayika no kwiheba muguhuza ibikorwa byakarere nka amygdala na hippocampus.
Kuvura Adjuvant Kuvura Indwara: Ifite agaciro gakoreshwa muburyo bwo kuvura ibimenyetso byamarangamutima / bihebye kandi birashobora gukoreshwa nkumuti wongeyeho. Indwara mbi ni indwara isanzwe yo mu mutwe, kandi uburyo bwo kuvura burimo ahanini kuvura imiti, kuvura indwara zo mu mutwe, no kuvura umubiri. Cort-g irashobora gukoreshwa nkimiti ivura, ihujwe nuburyo gakondo bwo kuvura kugirango tunoze ingaruka zo kuvura.
Mu gusoza, nka tetrapeptide ya sintetike, Cort-g ifite agaciro katagereranywa murwego rwibinyabuzima. Ku bijyanye no kurwanya gusaza, bifasha ADN gusubira mu rubyiruko mu kugenzura imiterere ya gene, bidindiza neza gusaza, kandi birashobora no kunoza imitekerereze ya metabolike mu bwonko iterwa na ischemia idakira. Mu rwego rwa neuroscience, irashobora guteza imbere ibikorwa bya neuron, bifite akamaro kanini mu gusana imvune no gukora ubushakashatsi ku ndwara zifata ubwonko. Igihe kimwe, irashobora kongera ubumenyi no kugabanya amaganya. Kubijyanye n'ubudahangarwa, birashobora gukangura imvugo ya interleukin-2 no kugenzura imikorere yubudahangarwa. Mubice byumutima nimiyoboro yubwonko, nubwo hakenewe ubundi bushakashatsi, birashobora kugira ingaruka kubipimo bifatika no kugenzura ingirabuzimafatizo z'umutima. Cort-g yafunguye icyerekezo gishya cyubuzima bwabantu kandi biteganijwe ko izagira uruhare runini mukuvura indwara hamwe no kunoza imikorere yumubiri.
Ibyerekeye Umwanditsi
Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.
Umwanditsi w'ikinyamakuru
Dr. Joseph I. Friedman ni umushakashatsi w'inzobere akaba n'inzobere mu by'ubuvuzi zifatanije n'ibigo bikomeye nka Mt. Sinayi Sisitemu y'ibitaro, Ishuri ry'ubuvuzi rya Icahn ku musozi wa Sinayi, n'ikigo cyita ku barwayi bo mu mutwe cya Piligrim. Ubushakashatsi bwe bwibanze mu bice bitandukanye birimo indwara zo mu mutwe, Neurosciences & Neurology, Pharmacology & Pharmacy, Cardiovascular System & Cardiology, na Medicine rusange. Yatanze umusanzu ukomeye mu gusobanukirwa no kuvura indwara zo mu mutwe, indwara z'umutima n'imitsi, hamwe n'ubumuga bwo kutamenya, hamwe n'ibitabo byinshi mu binyamakuru byasuzumwe. Ibikorwa bye byagize uruhare runini mu guteza imbere ubumenyi nubuvuzi muri ibi bice. Muganga Joseph I. Friedman yanditse kurutonde rwerekana [5].
