▎ Okunoonyereza ku Chonluten
Chonluten kye ki?
Chonluten ye peptide bioregulator okusinga eva mu bitundu by’ennyindo, erina emirimu mingi egy’ebiramu egisobola okukolebwa n’emiwendo gy’okukozesa.
Ensibuko y’okunoonyereza ku Chonluten eri etya?
1. Macro Background y’okunoonyereza ku Bioactive Peptides
Okuva mu kyasa eky’amakumi abiri, olw’okukulaakulana okw’amangu mu misomo nga molekyu biology ne biochemistry, bannassaayansi bakoze okunoonyereza okw’obwegendereza ku nsengekera n’emirimu gya molekyu ez’enjawulo mu biramu. Puloteeni, nga ebikulu ebisitula emirimu gy’obulamu, ensengekera za peptide ennyimpi ezikolebwa yuniti zazo ezikola - amino asidi zizuuliddwa nga zirimu amawulire amangi ag’ebiramu era nga zirina emirimu egy’enjawulo egy’omubiri. Mu nkola nnyingi enkulu ez’obulamu nga obubonero bw’obutoffaali, okulungamya enkyukakyuka mu mubiri, n’okuddamu kw’abaserikale b’omubiri, peptides ennyimpi zitera okukola omulimu oguteetaagisa. Okutegeera kuno kuleetedde ttiimu z’abanoonyereza mu nsi yonna okwenyigira ennyo mu kunoonyereza ku kusima peptides ennyimpi ezirina emirimu egy’enjawulo okuva mu bitundu by’ebiramu eby’enjawulo, nga basuubira okuleeta enkyukakyuka ez’obuyiiya mu bintu ebingi ng’obusawo, ssaayansi w’emmere, n’ebyobulimi. Okunoonyereza ku Chonluten kukolebwa ddala mu mbeera eno.
2. Omukisa gw’okuzuula Chonluten mu Bronchus
Mu kunoonyereza ku bitundu by’ebisolo bingi, ekitundu ky’ennyindo eky’enkola y’okussa kisikiriza bannassaayansi okufaayo okw’enjawulo. Ng’omukutu omukulu ggaasi okuyingira n’okufuluma mu mawuggwe, ekitundu ky’omubiri ekiyitibwa mucosal tissue of the bronchus kikwatagana butereevu n’obutonde obw’ebweru era kikola kinene nnyo mu kuziyiza okulumbibwa obuwuka obuleeta endwadde n’okukuuma obutebenkevu bw’embeera ey’omunda ey’enkola y’okussa.
Okusinziira ku ndowooza y’omubiri, obutoffaali bw’omubiri obuyitibwa epithelial cells of the bronchial mucosa buli kiseera buzzibwa buggya era ne buddaabirizibwa, era busobola okufulumya ebintu eby’enjawulo ebikola obulamu okulungamya obusimu obuziyiza endwadde obw’omu kitundu n’okuddamu kw’okuzimba, ekitegeeza nti waliwo emikisa mingi egy’okubeerawo kwa peptides ennyimpi ezirina emirimu egy’enjawulo mu kitundu kino. Okusinziira ku kino, ttiimu y’abanoonyereza yatwala ennywanto ng’ekifo we bayingira okunoonyereza era ne bakozesa tekinologiya ow’omulembe ow’ebiramu mu molekyu ne biochemistry okukola okunoonyereza okw’obwegendereza ku biva mu bitundu by’ennyindo. Oluvannyuma lw’okugezesa n’okwekenneenya okumala ebbanga eddene, Chonluten yasembayo okuzuulibwa obulungi. Okuzuula kuno tekukoma ku kuwa bubonero bupya ku kutegeera mu bujjuvu enkola y’okulungamya omubiri gw’ennyindo wabula era kuteekawo omusingi gw’okunoonyereza okuddirira ku nkozesa ya Chonluten mu kuziyiza n’okujjanjaba endwadde z’okussa n’ebyobulamu ebirala ebikwatagana nabyo.
Enkola ya Chonluten y’ekola etya?
1. Enkola y’okulungamya ku kuzimba
Okuziyiza okukola ekirungo ekiyitibwa Tumor Necrosis Factor (TNF) .
Tripeptide Chonluten esobola okuziyiza okukola tumor necrosis factor (TNF) okukolebwa monocytes oluvannyuma lw’okukwatibwa pro-inflammatory bacterial lipopolysaccharide (LPS) [1] . Okufulumya emiwendo emitono egya TNF kikwatagana n’enkola y’okugumira TNF, ekiyamba okukendeeza ku kuddamu kw’okuzimba.
Okusingira ddala, Chonluten eyinza okukosa okulaga kw’obuzaale oba emirimu gya puloteyina egyekuusa ku kukola TNF mu monocytes okuyita mu kkubo erimu ery’obubonero oba enkolagana ya molekyu, bwe kityo ne kikendeeza ku kusengejja n’okufulumya TNF.
Okuziyiza Okwolesebwa kwa Pro-inflammatory Cytokines
Khavinson Peptides zonna ettaano (nga mw’otwalidde ne Chonluten) zisobola okuziyiza okwolesebwa kwa TNF ne pro-inflammatory interleukin-6 (IL-6) cytokines mu butoffaali bwa THP-1 obwawuddwamu mu nkomerero obusikirizibwa LPS [1] . Kino kiraga nti Chonluten erina omulimu munene mu kuziyiza pro-inflammatory cytokines mu kuddamu kw’okuzimba.
Enkola esoboka eri nti Chonluten etaataaganya okusiba kwa LPS ku bikwata ku ngulu w’obutoffaali oba okulungamya ekkubo ly’okukyusa obubonero wansi, bwe kityo ne kikendeeza ku kukola kwa cytokines eziyamba okuzimba.
Okukendeeza ku kwekwata kw’obutoffaali
Nga tukuma obutoffaali bwa THP1 obujjanjabiddwa ne Chonluten ku layeri y’obutoffaali bw’omubiri (HUVECs) obukozesebwa LPS, okukendeera mu kwekwata kw’obutoffaali kwalabiddwa [1] . Okunywerera kw’obutoffaali nkola ya bulijjo eyamba okuzimba, era okukendeeza ku kwekwata kw’obutoffaali nga Chonluten kuyinza okuyamba okukendeeza ku kuddamu kw’okuzimba.
Kino kiyinza okuba nga kiva ku Chonluten ekyusa okwolesebwa oba emirimu gya molekyo z’okungulu kw’obutoffaali, bwe kityo n’ekosa enkolagana wakati w’obutoffaali oba wakati w’obutoffaali ne matrix.
2. Enkola y’okulungamya ku nkola y’okusaasaana
Okwongera ku Tyrosine Phosphorylation
Okufaananako ne Khavinson Peptides endala, Chonluten esobola okwongera ku tyrosine phosphorylation ya mitogen-activated cytoplasmic kinases [1] . Kino kiraga nti Chonluten esobola okulungamya ekkubo ly’obubonero obukulu mu nkola y’okukula kw’obutoffaali. Tyrosine phosphorylation y’emu ku nkola enkulu ez’okukyusa obubonero mu butoffaali, eyinza okukola oba okuziyiza emirimu gya puloteyina ez’enjawulo, bwe kityo ne kikosa okukula kw’obutoffaali, okwawukana, n’okuwangaala. Chonluten eyinza okwongera ku tyrosine phosphorylation ya cytoplasmic kinases nga etereeza emirimu gya kinases oba phosphatases ebitongole, n’oluvannyuma n’ekosa enkola y’okukula kw’obutoffaali.
3. Omulimu mu Cervical Mucus
Mu kunoonyereza okwakolebwa ku bakyala 14 abajjanjabwa ne Conluten (kiyinza okuba nga yeekuusa ku Chonluten) n’abakyala 9 abajjanjabwa ne Conlunett, kyazuulibwa nti bwe kyazuulibwa mu ngeri ya nuclear magnetic resonance (NMR), mu kiseera ky’obujjanjabi, eddagala lyombi liyinza okukyusa omusulo gw’omumwa gwa nnabaana okuva mu kika kya estrogen (ekika kya E) ekisobozesa ensigo okusenguka okudda mu kika kya progesterone (ekika kya G) ekiziyiza ensigo okusenguka mu kiseera ky’okuggyamu eggi. Enkyukakyuka eno yabaddewo mu bakyala 74% mu kibinja kya Conluten ne 64% ku bakyala mu kibinja kya Conlunett [2]..
Biki ebikozesebwa mu Chonluten?
Obujjanjabi bw’obulwadde bw’amawuggwe obutawona (COPD) ne Asima: Chonluten eraga obusobozi bw’okujjanjaba mu ndwadde z’okussa nga obulwadde bw’amawuggwe obutawona n’asima. Okunoonyereza kulaga nti asobola okukendeeza ku buzimba [1] . Okuzimba nsonga nkulu mu ntandikwa y’obulwadde bwa COPD ne asima. Nga akendeeza ku kuzimba, Chonluten asobola okumalawo obubonero ng’okusannyalala kw’emikutu gy’empewo n’okusannyalala. Mu kiseera kye kimu, ekizibu kino ekya peptide complex era kisobola okutumbula okuddaabiriza n’okuzza obuggya ebitundu by’amawuggwe. Okwonooneka kw’ebitundu by’amawuggwe kujja kuleetera enkola y’okussa okukendeera, era ekikolwa ky’okuddaabiriza ekya Chonluten kisobola okulongoosa ensengekera n’enkola y’amawuggwe, bwe kityo ne kyongera ku nkola y’okussa.
Okuddaabiriza obuvune mu lubuto: Chonluten eraga obusobozi mu kuddaabiriza obuvune bw’omu lubuto. Kiyinza okulungamya okulaga kw’obuzaale obukwatagana n’enziyiza eziziyiza obuwuka obuleeta obulwadde (nga superoxide dismutase) [1] . Enkola y’okuziyiza obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obuwuka obuleeta obulwadde mu lubuto. Enkola y’okulungamya eya Chonluten esobola okuyamba okuzzaawo bbalansi y’enkola y’okuziyiza obuwuka obuleeta obuwuka n’okukendeeza ku kwonooneka kw’okunyigirizibwa kw’okwokya (oxidative stress) ku mucosa y’olubuto. Okugatta ku ekyo, ekizibu kino ekya peptide kisobola n’okutumbula okuddamu okuzimba obutoffaali n’okwanguyiza enkola y’okuddaabiriza obuvune bw’omubiri gw’omu lubuto.
Mu kumaliriza, Chonluten eraga emiwendo egisobola okukozesebwa mu bujjanjabi mu by’okulwanyisa okuzimba, okuddaabiriza ebitundu, n’okulungamya abaserikale b’omubiri.
Ebikwata ku Muwandiisi
Ebintu ebyogeddwako waggulu byonna binoonyerezeddwako, birongooseddwa era ne bikung’aanyizibwa kkampuni ya Cocer Peptides.
Omuwandiisi wa Scientific Journal
Francesco Avolio musomesa akwatagana ne G. d’Annunzio University of Chieti-Pescara, naddala mu kitongole ekikola ku by’okunoonyereza eby’omulembe ne tekinologiya (Ctr Adv Studies & Technol). Okunoonyereza kwe kukwata ku bintu bya ssaayansi ebiwerako, omuli Research & Experimental Medicine, Biochemistry & Molecular Biology, Physiology, Immunology, ne Endocrinology & Metabolism. Ng’omu ku banoonyereza mu yunivasite eno, ayambako mu kussa essira ku ttendekero lino okutumbula okumanya n’obuyiiya mu bya ssaayansi. Francesco Avolio awandiikiddwa mu kiwandiiko ekijuliziddwa [1].
