Peptide kunnafoni fɛ 
Awirilikalo tile 21, san 2025
BƐƐRƐ KUNNAFONIW NI FƐRƐW KUNNAFONIW BƐƐ MIN BƐ NI WEBSITE KAN NA, U BƐ KUNNAFONIW FƆLƆ DƆRƆN NI KALANSENW YE.
Fɛn minnu bɛ di nin siti in kan, olu dabɔra ɲininiw dɔrɔn kama in vitro. Sɛgɛsɛgɛli in vitro (latinkan na: *in glass*, o kɔrɔ ye gilasi minɛnw na) bɛ kɛ hadamaden farikolo kɔkan. Nin fura ninnu tɛ furaw ye, Ameriki dumuniko ni furako ɲɛmɔgɔso (FDA) ma sɔn u ma, wa u man kan ka kɛ ka furakɛli bana, bana walima bana si bali, k’a furakɛ, walima k’a furakɛ. A dagalen don kosɛbɛ sariya fɛ ka nin fɛn ninnu don hadamaden walima bagan farikolo la cogo o cogo.
Asidi aminikiw ye fɛnɲɛnamaw ye minnu kɔnɔ amino kulu ni karɔbɔli kulu bɛ fara ɲɔgɔn kan ni kɛrɛfɛ cakɛda ye min bɛ danfara.
Asidi α-amino minnu bɛ pepitiriw ni poroteyini minnu dilannen don ni ribozomi ye, olu fanba la, o kuluw bɛ siri α-karɔbɔni atomu kelen na. Jɛkuluba sigicogo bɛ se ka jira nin cogo la:
H2N–CH(R)–COOH ye fɛn ye min bɛ se ka kɛ
yɔrɔ min na R bɛ kɛrɛfɛ cakɛda jira.
Kɛrɛfɛ cakɛda de bɛ asidi aminiki kelen ni tɔ kelen danfara ani ka nɔba bila jogo dɔw la i n’a fɔ charge, polarite, hydrophobicity, steric behavior, ani kemikali reactivité.
Amino acide standard mugan bɛ kodɔn tuma caman na ni canonical genetic code ye ani u bɛ baara kɛ kosɛbɛ ribosomal protein synthesis la. Asidi aminiki wɛrɛw minnu bɛ sɔrɔ jamu fɛ, i n’a fɔ selenocysteine ani pirrolysine, olu bɛ sɔrɔ fɛnɲɛnamaw ka sigida kɛrɛnkɛrɛnnenw na.
Pepitiriw ka chimie ta fan fɛ, asidi aminikiw bɛ faamuya ka ɲɛ i n’a fɔ molekiyɔmu jɔlifɛnw, pepitiri sinsinni ɲɛfɔlenw bɛ se ka lajɛ minnu na.
Pepitiri ye fɛn ye min bɛ kɛ ni asidi aminiki tolenw ye minnu bɛ ɲɔgɔn sɔrɔ pepitiri jɛɲɔgɔnyaw fɛ.
Pepitiri jɛɲɔgɔnya ye amidi jɛɲɔgɔnya ye min bɛ kɛ tolen kelen ka karɔbɔli kulu min bɔra karɔbɔli la ani tolen nata ka azote sɔrɔlen amino cɛ.
Ni asidi aminikiw donna pepitiri cakɛda dɔ kɔnɔ, u bɛ wele ka ɲɛ kosɛbɛ ko asidi aminiki tolenw.
O la, ɲɔgɔndan nɔgɔman bɛ se ka jira i n’a fɔ:
Asidi aminiki → Peptide sirili → Asidi aminiki tolen → Peptide cakɛda
Pepitidiw bɛ se ka tolen damadɔ dɔrɔn de sɔrɔ walima u sigiyɔrɔma minnu ka jan kosɛbɛ.
Daɲɛw i n’a fɔ dipɛtidi , tripɛtidi , ani oligopɛtidi ye ɲɛfɔli nafamaw ye, nka tolen hakɛ sigilen tɛ diɲɛ bɛɛ kɔnɔ, pepitiri kelen-kelen bɛɛ ka kan ka barika sɔrɔ ka jate polipɛtidi walima poroteyini ye.
Danfara bɛ bɔ dɔnniya siratigɛ la, sigicogo labɛnni na ani bɛnkan na sanni ka da jatebɔ dan kelen kan.
Asidi aminikiw ni pepitiriw bɛ ɲɔgɔn na kosɛbɛ, nka u tɛ daɲɛw ye minnu bɛ se ka ɲɔgɔn falen.
Asidi aminiki hɔrɔnma ye molekiyɔmu suguya kelen ye.
Pepitiri ye fɛnɲɛnamafagalan ɲɛfɔlen ye min kɔnɔ asidi aminikiw tolen caman bɛ ɲɔgɔn sɔrɔ kovalɛn na ɲɔgɔndan kɛrɛnkɛrɛnnen dɔ la.
O danfara in nafa ka bon bawo asidi aminikiw sirili ɲɔgɔn na, o bɛ jogo dɔw Dabɔ minnu tɛ Se ka Fɔ ka ɲɛ ni asidi aminikiw kelen-kelen bɛɛ lajɛli ye dɔrɔn.
Ni u donna pepitiri cakɛda dɔ kɔnɔ, tolenw bɛ nɔ bila ɲɔgɔn na ni ninnu ye:
kuran-sɛnɛ-ɲɔgɔnnaw
idɔrɔzi sirili
jilafɛnw ka jɛɲɔgɔnyaw
steriki nɔ minnu bɛ sɔrɔ
aromatiki jɛɲɔgɔnyaw
konformasiyɔn dantigɛliw
fɛn caman b’a la ka fɛn caman sɛmɛntiya
O la, o ɲɔgɔndan bɛ kɛ pepitiri ka farikoloɲɛnajɛ bɛɛ lajɛlen kɛcogo dantigɛlanba ye.
Dɔnniya daɲɛw bɛ asidi aminiki hɔrɔnma ni asidi aminiki tolen danfara Bɔ pepitiri kɔnɔ.
Misali la, glycine hɔrɔnma ye asidi aminiki ye.
Ni glycine donna pepitiri kɔnɔ pepitiri-joli sɔrɔli fɛ, cakɛda yɔrɔ min bɛ sɔrɔ glycine fɛ, o bɛ wele ko glycine tolen.
Daɲɛ 'residu' bɛ a jira ko a donli pepitiri kɔnɔ, o bɛ asidi aminiki fɔlɔ ka kemikaliya cogoya Changer.
O danfara in bɛ kɛ nafama ye kɛrɛnkɛrɛnnenya la ni i bɛ molekiyɔmuw hakɛ ɲɛfɔ, ni u bɛ tugu ɲɔgɔn kɔ, ni pepitiriw dilanni, walima ni u jɔcogo sɛgɛsɛgɛli bɛ kɛ.
Pepitiri kɔkolo bɛ segin-ka-bɔ min bɛ kɛ fɔlɔ ni:
–N–Cα–C(=O)– Bamako, Mali.
unité (unit) minnu bɛ yen.
Min nɔrɔlen bɛ α-karɔbɔni kelen-kelen bɛɛ la, o ye o tolen kɛrɛfɛ cakɛda taamaʃyɛn ye.
Pepitiri kɔkolo bɛ sigicogo jɛlen di, ka sɔrɔ kɛrɛfɛ cakɛdaw bɛ kemikɛli suguya caman di.
Pepitiri sinsinni fanba fana bɛ ni dakun fila ye minnu tɛ kelen ye kemikaliya siratigɛ la:
N-terminus — laban min bɛ tali kɛ terminal amino kulu la.
C-terminus — laban min bɛ tali kɛ terminal carboxyl kulu la.
Peptide sinsinniw bɛ sɛbɛn cogoya la ka bɔ N-terminus la ka taa C-terminus la.
I n'a fo:
Ala–Gly–Ser ye baara kɛ
bɛ tripeptide dɔ ɲɛfɔ min kɔnɔ alanini ye N-terminal tolen ye ani serine ye C-terminal tolen ye.
Jɛkulu labanw fana bɛ se ka fɛn caman sɛmɛntiya kemikɛli fɛ, o la ɲinini pepitiri bɛɛ tɛ wajibiya ka amino kulu hɔrɔnma walima karɔkisili kulu hɔrɔnma sɔrɔ a dakunw na.
Pepitiri kɔkolo bɛ ɲɔgɔn ta a jɔcogo la ɲɔgɔndan bɛɛ kɔnɔ, nka asidi aminikiw kɛrɛfɛ cakɛdaw bɛ ɲɔgɔn ta kosɛbɛ.
O danfara ninnu bɛ nɔba bila pepitiriw kɛcogo la.
Fɛn tolenw i n’a fɔ leucine, isoleucine, valine, phenylalanine ani methionine, olu bɛ se ka kɛ sababu ye ka jilafɛnw ka ɲɔgɔn sɔrɔ.
Pepitiri min kɔnɔ ji-sɔrɔ-fɛn caman bɛ sɔrɔ, o bɛ se ka kɛ ni ji ye cogo ɲuman na, wa a bɛ se ka dɔ fara a yɛrɛ ka jɛɲɔgɔnya kan cogoya dɔw la.
Nka, jiko bɛ bɔ ɲɔgɔndan bɛɛ la sanni ka bɔ tolen kelen si sɔrɔli la.
Tolenw i n’a fɔ serini, treyonini, asparagine ani glutamine, kɛrɛfɛ cakɛdaw bɛ u la minnu bɛ se ka u sen don idɔrɔzi ni ɲɔgɔn cɛ jɛɲɔgɔnyaw la.
O tolenw bɛ se ka nɔ bila jiko la, molekiyɔmuw dɔnni na, sigida cogoya la, ani u ka wulicogo la.
Dɔw fana bɛ se ka u sen don kemikɛli walima anzimu caman cili la ka kɛɲɛ ni ɲɛnamaya walima kɔrɔbɔli kɛcogo ye.
Asidi aspartitiki ni asidi glutamiki bɛ kɛ kɛrɛfɛ cakɛdaw la minnu kɔnɔ karɔbɔli bɛ sɔrɔ.
U ka protonation (protonation) cogoya bɛ Bɔ pH (pH) de la.
Kɔrɔbɔli cogoya caman na minnu bɛ kɛ ka caya, o tolenw bɛ charge négative di pepitiri ma.
O la sa, u bɛ nɔ bila:
net charge, isoelectric behavior, solubility, chromatographie retention, ani molekiyɔmuw ka jɛɲɔgɔnyaw.
A ka c’a la, lisini ni arijini bɛ charge positif di laboratuwari cogoya caman na, ka sɔrɔ histidine bɛ protonation kɛcogo jira min bɛ tali kɛ cogoya la kosɛbɛ pH hakɛw kɛrɛfɛ minnu bɛ tali kɛ farikolo ni kɔrɔbɔli la.
O tolenw bɛ se ka nɔba bila pepitiri charge (pepitiri charge) la ani u ni ɲɔgɔn cɛ ni molekilɛri wɛrɛw ye walima ni fasa jɔlenw ye minnu bɛ kɛ ni chromatographie ye.
Fenilalanini, tirosini ani triptofan bɛ kɛ kɛrɛfɛ cakɛda kasadimanw na.
Tolenw kasa dumanw bɛ se ka u sen don jilafɛnw ni π-ɲɔgɔnna jɛɲɔgɔnyaw la ani u bɛ se ka kɛ sababu ye ka UV minɛ.
Nka, kuma min bɛ fɔ ko asidi aminiki kasadimanw bɛ caman kɛ ka fɛnw minɛ '280 nm' kɛrɛfɛ, o ka kan ka baara kɛ ni hakili ye.
Triptophane ni tyrosine bɛ dɛmɛ kosɛbɛ ka fɛnw minɛ ni 280 nm kɛrɛfɛ, k’a sɔrɔ fenilalanini bɛ minɛ ka dɔgɔn kosɛbɛ o yɔrɔ la.
O nafa ka bon ni UV spectroscopie bɛ kɛ ka pepitiri hakɛ jateminɛ.
Barokun fɔlɔ ye asidi aminiki kasadimanw bɛɛ furakɛ i n’a fɔ u bɛ dɛmɛ don o cogo kelen na ~280 nm minɛni na. Daɲɛ minnu labɛnna kokura, olu b’o danfara kɛ ka ɲɛ.
Pepitiri fila bɛ se ka kɛ ni asidi aminiki kelenw ye tigitigi nka u kɛcogo tɛ kelen ye ni o tolenw bɛ sɔrɔ cogo wɛrɛ la.
I n'a fo:
A–B–C–D ye
ani
D–C–B–A ye
u ka asidi aminiki hakɛ ye kelen ye nka u sigiyɔrɔma tɛ kelen ye.
Yɛlɛma min bɛ kɛ ɲɔgɔn kɔ, o bɛ se ka fɛn caman Changer:
sara tilatilali
ji-sɔrɔ-yɔrɔ cogoya
konformasiyɔn ka ɲɛnatɔmɔli
farali ŋaniya
solubility (sɔgɔli) ye
molekiyɔmuw dɔnni
chromatographie kɛcogo
O la, asidi aminiki hakɛ dɔrɔn tɛ se ka pepitiri dɔ ɲɛfɔ.
ye Tolenw sigicogo ko jɔnjɔn ye.
O labɛncogo sigilen in bɛ Weele ko pepitiri ka jɔcogo fɔlɔ.
Ni dɔ farala pepitiri janya kan, o tɛ 'tɛmɛsira nɔgɔman dɔ Dabɔ ka Bɔ 'labɛnbali' la ka Taa 'daɲɛw la.' la.
Pepitiri surunw bɛ se ka cogoya ɲumanw ta cogo dɔw la, ka sɔrɔ pepitiri janw bɛ se ka to fanga la kosɛbɛ.
Ka kɛɲɛ ni ɲɔgɔndan ni sigida ye, pepitiriw bɛ se ka kɛ ni sigicogo motifw ye walima ka u ta waati dɔɔnin kɔnɔ i n’a fɔ:
α-helical segments (yɔrɔw) minnu bɛ wele ko α-helical segments
β-strand-like conformations (daɲɛ minnu bɛ i n’a fɔ β-strand).
β-yɔrɔ-yɔrɔ-ko
loops (lupulu)w
conformations (cogow) minnu tɛ labɛn
Fɛnɲɛnɛma, funteni, pH, a hakɛ, ion fanga, ani jɛɲɔgɔnya ni molekiyɔrɔ wɛrɛw ye, olu bɛɛ bɛ se ka nɔ bila konformasiyɔn ansamblɛ la.
I ka barokun fɔlɔ y’a jira ko pepitiri surunw bɛ se ka wuli ka bɔ u nɔ na tuma caman na k’a sɔrɔ pepitiri janw bɛ sigida filananw dilan ani 'u tɛ ni sigida saba sigicogo sabatilenw ye.' O ɲɛfɔli ka gɛlɛn kojugu.
Peptide jɔcogo ka kan ka faamuya o nɔ na i n’a fɔ cogoya kɛcogo taabolo min bɛ tali kɛ kosɛbɛ ɲɔgɔndan ni sigida la.
Pepitiri bɛɛ min bɛ kɛ kemikɛli ɲininiw na, o bɛɛ tɛ kɛ ni amino asidi mugan ye minnu bɛ wele ko standard proteinogenic amino acids.
Bi pepitiri chimie bɛ se ka ninnu don a kɔnɔ:
D-amino acides (Amino acides) minnu bɛ sɔrɔ
asidi aminiki minnu tɛ farikolojɔlifɛnw ye
N-methylated tolenw
Asidi β-aminofɛnw (amino acides).
tolenw minnu sɛgɛsɛgɛra kemikɛli fɛ
asidi aminikiw minnu bɛ taamasiyɛn kɛ isotopu la
tolenw minnu kɔnɔ baarakɛjɛkulu kɛrɛnkɛrɛnnenw bɛ sɔrɔ
O fɛn caman b’a To ɲininikɛlaw bɛ Se k’a Sɛgɛsɛgɛ cogo min na fɛn minnu bɛ Yɛlɛma molekiyɔmuw jɔcogo la, olu bɛ nɔ Blà pepitiriw ka jogo la.
Nafa min b’a la, o ye ko ni tolen dɔ donna a kɔnɔ, o bɛ pepitiri dɔ dilan min ni kemikaliya tɛ kelen ye.
Fɛn min bɛ sɔrɔ o la, o bɛ se ka kɛ danfara ye molekiyɔmu hakɛ la, charge (sara) la, u ka wulicogo la, u cogoya la, u ka chromatographie (chromatographie) maracogo la, u ka se ka tiɲɛni kɛ, ani u ka sumanikɛcogo wɛrɛw la.
Asidi aminikiw fanba minnu bɛ don a la ribosomal proteine synthèse normale waati la, olu bɛ sɔrɔ L-configuration kɔnɔ.
Nka, D-amino acids fana bɛ sɔrɔ fɛnɲɛnamaw la, wa u bɛ se ka don a la ni laɲini ye pepitiri kemikaliw dilanni senfɛ.
L-tolen dɔ nɔnabila ni D-tolen ye min bɛ bɛn o ma, o bɛ stɛriyɔmu caman Changé k’a sɔrɔ a ma fɛnɲɛnɛmaw ka danfara Changé.
O fɛnɲɛnɛma-yɔrɔ-ko-yɔrɔ-ko min bɛ i n’a fɔ a ka dɔgɔ, o bɛ se ka nɔba bila sigida cogoya ni molekiyɔmuw ka jɛɲɔgɔnyaw la.
O la, stɛriyɔmu ye pepitiriw dɔnni yɔrɔ nafamaba ye.
Pepitiriw sigiyɔrɔma ɲɛfɔli ka kan ka danfara stɛriyɔmuw jira cogo ɲuman na tuma o tuma ni cogoya minnu tɛ sariya ye, olu bɛ yen.
Ribozomu bamanankan bamanankan na, asidi aminikiw bɛ nɔrɔ fɔlɔ u ka ARNw la minnu bɛ bɛn u ma, ka aminoasili-tRNAw dilan.
O kɔfɛ, ribozomu bɛ baara kɛ ni ɲɔgɔndan ye min kofɔlen don ciden ARN fɛ walasa ka o aminoacyl-tRNA ninnu labɛn ani ka pepitiri cakɛda min bɛ ka bonya, o janya.
α-amino kulu min bɛ tali kɛ aminoacyl-tRNA la A yɔrɔ la, o bɛ bin peptidyl-tRNA ka carbonyl cɛmancɛ kan P yɔrɔ la, ka cakɛda min bɛ ka bonya, o jiginni kɛ ka pepitiri jɛɲɔgɔnya nata kɛ.
Pepitiri cakɛda bɛ lajɛ ka bɔ N-yɔrɔ la ka taa C-yɔrɔ la.
Barokun fɔlɔ b'a fɔ ko 'asidi aminiki bɛ don ribozomu kɔnɔ,' o ye nɔgɔya kojugu ye. Ni an b’a fɔ ka ɲɛ, asidi aminikiw bɛ lase ribozomu ma i n’a fɔ aminoasili-tRNAw.
Pepitidiw fana bɛ se ka lajɛ ni kemikɛli ye ni ribosomali masinw tɛ.
Pepitiriw dilanni na min bɛ kɛ fasa gɛlɛn na, asidi aminikiw bɔlenw lakananenw bɛ fara ɲɔgɔn kan pepitiri cakɛda la min sirilen bɛ resin na min bɛ ka bonya.
O bɛ ɲininikɛla bila ka fanga tilennen di ɲɔgɔndan laɲininen ma ani fana ka sira di tolenw walima fɛn caman caman cili ma minnu bɛ se ka don nɔgɔya la ribozoma bamanankan baarakɛcogo jɔnjɔn fɛ.
Nafa bɛ sɔrɔ kemikaliya la kɛrɛnkɛrɛnnenya la ɲinini pepitiri ɲɛfɔlenw na, nka a gɛlɛya waleyali bɛ bɔ ɲɔgɔn kɔ kosɛbɛ.
barokun fɔlɔ y' a jira ko kemikɛli kɛcogo bɛnnen don pepitiriw ma minnu bɛ tolen 50 duguma . O man kan ka jate i n’a fɔ dan sigilen.
Siga t’a la, pepitidi minnu janya ka ca ni tolen 50 ye, olu bɛ se ka dilan ni kemikɛli ye, hali ni u janya bɛ bonya tuma caman na, o bɛ dɔ fara gɛlɛyaw kan minnu bɛ sɔrɔ ɲɔgɔndan nafama na, u dalajɛli, u saniyacogo ani u dalajɛli la minnu bɛ tali kɛ ɲɔgɔn kɔ.
Ni ɲɔgɔndan gɛlɛnw walima minnu ka jan, ɲininikɛlaw bɛ se fana ka baara kɛ ni segin-ka-bɔnyew ye, ka baara kɛ ni segin-ka-bɔnyew ye, walima ni fɛɛrɛw ye minnu bɛ kɛ ɲɔgɔn fɛ.
Pepitiri dɔw minnu bɛ sɔrɔ u yɛrɛ la, olu tɛ dilan k’a ɲɛsin ribozoma bamanankan baarakɛcogo ma.
Misali la, fɛnɲɛnama misɛnninw bɛ se ka baara kɛ ni anzimu caman ye minnu bɛ wele ko non-ribosomal peptide synthetases (NRPS) walasa ka pepitiri fɛnw lajɛ minnu jɔcogo tɛ kelen ye.
O sigida ninnu bɛ se ka tolenw ni u jɔcogo dɔw don a kɔnɔ minnu ni ribozoma pepitiri kɔrɔlenw tɛ kelen ye kosɛbɛ, i n’a fɔ:
asidi aminiki minnu tɛ farikolojɔlifɛnw ye
D-amino acides (Amino acides) minnu bɛ sɔrɔ
sikɔlɔ la
metilasi (méthylation) ye
bεε bεε bε bεn kɛrɛnkɛrɛnnenya la
O b’a jira ko pepitiriw ka chimie bɛ tɛmɛn kosɛbɛ asidi aminikiw mugan kan minnu bɛ don a la cogoya la ribozom poroteyini sɔrɔli waati.
Pepitiri ka charge net bɛ bɔ kulu ionizelenw na minnu bɛ a sinsinni na ka fara a lamini pH kan.
Jɛkulu minnu bɛ se ka ionize, olu bɛ se ka kɛ nin yɔrɔ ninnu na:
N-terminus (N-yɔrɔ) ye
C-terminus (C-terminus) ye
asidi kɛrɛfɛ cakɛdaw
kɛrɛfɛ cakɛdaw basigilenw
baarakɛjɛkulu wɛrɛw sugandira
Ni pH bɛ Changé, o kuluw ka protonation cogoya bɛ se ka Changé.
O de kosɔn, pepitiri kelen bɛ se ka charge net (sara) suguya wɛrɛw ta k’a kɛ kɔrɔbɔli cogoya wɛrɛw la.
O nafa ka bon walasa ka faamuyali sɔrɔ:
solubility, ion-exchange chromatography, electrophoretic behavior, molekiyɔmuw ka jɛɲɔgɔnyaw, ani ɲɔgɔndan.
A b’a ɲɛfɔ fana mun na pepitiri charge man kan ka ɲɛfɔ abada ni pH ma jateminɛ.
Kɛrɛfɛ cakɛda danfara fana bɛ dɛmɛba lase solubility ma.
Pepitiri min falen don chargement ni polar tolenw na, o bɛ se ka baara kɛ koɲuman ni jilama ye, ka sɔrɔ pepitiri min kɔnɔna ka bon ji mabɔ, o bɛ se ka gɛlɛya ka tɛmɛ a marali kan jilama kɔnɔ.
Nka tolenw jate nɔgɔman tɛ bɔli kɛ.
Charge ni jilafɛnw jɔyɔrɔ, pepitiri hakɛ, pH, ion fanga, conformational behavior, ani aggregation propensity, olu bɛɛ bɛ se ka nɔ bila jateminɛ kɔlɔsilen na.
O de y’a to pepitiri fila minnu girinya bɛ ɲɔgɔn ta, olu bɛ se ka wulicogo jira minnu tɛ kelen ye kosɛbɛ.
Asidi aminikiw sɔrɔli fana bɛ nɔ bila pepitiri bɛ a yɛrɛ minɛ cogo min na sɛgɛsɛgɛli sɛgɛsɛgɛli senfɛ.
I n'a fo:
RP-HPLC kɛcogo bɛ nɔba bila jiko bɛɛ lajɛlen na ani jɛɲɔgɔnya min bɛ tali kɛ ɲɔgɔndan na ni fasa jɔlen ye.
Ion-exchange chromatography bɛ bɔ pepitiri charge la tampon cogoya sugandilenw na.
Massa spectrometry bɛ molekiyɔmu masɔrɔ ni ionize kɛcogo jira.
UV spectroscopie bɛ bɔ yɔrɔ dɔ la UV-absorbing residues walima chromophores wɛrɛw sɔrɔli la.
O la, pepitiri ka asidi aminikiw sigiyɔrɔma bɛ tali kɛ sɛgɛsɛgɛli fɛɛrɛw la minnu bɛ kɛ ka a jogo jira.
Yɛlɛma bɛ se ka kɛ pepitidiw la ka fara u ka asidi aminiki fɔlɔw sigiyɔrɔma kan.
Misali dɔw ye ninnu ye:
fosforilasi (fosforilasi) ye
asetili (asetilasi) ye
amidation (amidasiyɔn) ye
metilasi (méthylation) ye
glycosylation (glycosylation) ye
disulfide (disulfide) sɔrɔli
lipidation (sɔgɔsɔgɔninjɛ).
O bεε bε Se ka bεnkansεbεn, charge, hydrophobicity, conformation ani chromatographie kɛcogo Changer.
Yɛlɛma bɛɛ tɛ Kɛ a yɛrɛ la, wa tolen bɛɛ tɛ Yɛlɛma cogoya bɛɛ la.
Barokun fɔlɔ bɛ serini ni treyoni fosforilasi ni asparagine glycosylation ɲɛfɔ i n’a fɔ pepitiriw ka jogo caman. Olu bɛ ɲɛfɔ ka ɲɛ i n’a fɔ fɛn caman b’a la ka Changements possibles dans les contextes biologiques walima synthétiques appropriés , sanni ka kɛ o tolenw ka nafaw ye minnu bɛ sɔrɔ u yɛrɛ la.
Pepitiri man kan ka jateminɛ ni ɲininkali dɔrɔn ye ko asidi aminiki hakɛ min bɛ a la.
Ɲɛfɔli dafalen dɔ bɛ yen:
Sequence
Tolen jumɛnw bɛ yen ani u bɛ kɛ cogo jumɛn na?
Stereochimie
Yala tolenw L-, D-, walima u bε bεn cogo wɛrɛ la wa?
Termini
Yala N- ni C-termini tɛ kelen ye wa walima u bɛ fɛn dɔw sɛmɛntiya kemikɛli fɛ wa?
Covalent Modifications
Yala disulfide jɛɲɔgɔnyaw, fosforilasi, amidation walima fɛn wɛrɛw bɛ yen wa?
Molecules Mass
Yala molecule mass sumanlen bɛ bɛn ni sigicogo makɔnɔnen ye wa?
Saniyali
Profil chromatographique analytique hakɛ jumɛn bɛ bɛn a yɔrɔba ma?
O jogo ninnu faralen ɲɔgɔn kan, u bɛ pepitiri ɲɛfɔli kɛ min kɔrɔ ka bon dɔnniya siratigɛ la ka tɛmɛ tolenw jate dɔrɔn kan.
Asidi aminikiw bɛ daɲɛw di kemikaliya la, pepitiriw bɛ dilan ka bɔ minnu na.
Peptide sirili bɛ o asidi aminiki tolenw labɛn ka kɛ ɲɔgɔndan ɲɛfɔlenw ye, k’a sɔrɔ u kɛrɛfɛ cakɛdaw ka kemisɛri de bɛ molekiyɔmuw kɛcogo caman latigɛ minnu bɛ sɔrɔ o la.
O jɛɲɔgɔnya bɛ se ka fɔ ka surunya cogo nɔgɔman na:
Asidi aminikiw dɔnni → A sigiyɔrɔma → Molecules structure → Physicochimique properties → Kɔrɔbɔli kɛcogo
O jɛɲɔgɔnya faamuyali ye fɛnba ye pepitiriw dilanni na, u saniyacogo la, u wulicogo la, u maracogo la, ani u sɛgɛsɛgɛli kɛcogo la.
A b’a ɲɛfɔ fana mun na a bɛ iko fɛn minnu bɛ ɲɔgɔn falen-falen misɛnninw, olu bɛ se ka danfara kɔrɔw lase pepitiri fɛn fila ni ɲɔgɔn cɛ.
O la, laboratuwari ɲininiw kama, pepitiri ka kan ka jate i n’a fɔ molekiyɔmu dafalen sanni ka jate asidi aminikiw kelen-kelen bɛɛ lajɛlen dɔrɔn ye.
Cocer Peptides bɛ fɛn minnu di, olu dabɔra laboratuwari ɲininiw dɔrɔn kama, sɛgɛsɛgɛli sɛgɛsɛgɛli, ani sɛgɛsɛgɛli in vitro. U ma dabɔ hadamadenw walima baganw ka furakɛli kama, ka u dunni, ka bana sɛgɛsɛgɛ, ka u furakɛ, ka u kunbɛn, walima ka u kɛ kɛnɛyaso la.
Dɔnniya ni kalan kunnafoniw minnu bɛ sɔrɔ nin siti in kan, olu dabɔra ɲininiw kama, wa u man kan ka kɔrɔfɔ iko furakɛli ladilikanw walima iko cikanw hadamadenw walima baganw baaracogo kan.
