▎ Vilon Research
Dab tsi yog qhov kev tshawb fawb keeb kwm ntawm Vilon?
1. Kev Tshawb Fawb Txog Kev Laus thiab Kab Mob
Nrog rau kev nrawm ntawm cov txheej txheem kev laus hauv ntiaj teb, ntau cov teeb meem cuam tshuam txog kev laus, xws li cellular senescence, poob ntawm cov ntaub so ntswg ua haujlwm, thiab qhov tshwm sim siab ntawm cov kab mob geriatric, tau dhau los ua cov teeb meem kho mob ceev kom daws tau. Cellular senescence yog nrog los ntawm kev maj mam poob ntawm lub peev xwm los tswj homeostasis thiab kho cov ntaub so ntswg puas, ua rau nws tseem ceeb heev kom nrhiav tau cov tshuaj uas tuaj yeem ncua cov txheej txheem ntawm cellular senescence thiab txhim kho cov ntaub so ntswg.
Tib lub sijhawm, cov kab mob loj xws li mob qog noj ntshav, ntshav qab zib, kab mob plawv, thiab kab mob siab ua rau tib neeg noj qab haus huv. Cov kev kho mob ib txwm muaj qee yam kev txwv hauv kev daws cov kab mob no, thiab muaj qhov xav tau sai los tsim cov kev kho tshiab thiab tshuaj. Piv txwv li, cov neeg mob qog noj ntshav laus muaj kev ua siab ntev rau kev siv hluav taws xob thiab tshuaj khomob, thiab kev kho mob tsis txaus siab. Nws yog qhov ceev kom nrhiav kev nyab xeeb thiab zoo adjuvant txoj kev kho mob. Nyob rau hauv qhov xwm txheej nyuaj, cov kws tshawb fawb tau tig lawv lub siab rau bioactive peptides, vam tias yuav nrhiav tau lub ntsiab lus tawg, thiab kev tshawb fawb ntawm Vilon tau los ua.
2. Kev Loj Hlob ntawm Kev Tshawb Fawb Txog Bioactive Peptides
Cov tshuaj Peptide muaj dav nyob rau hauv cov kab mob uas muaj sia nyob thiab koom nrog ntau cov txheej txheem physiological tseem ceeb, xws li kev taw qhia ntawm tes, kev tiv thaiv kab mob, thiab kev tswj cov metabolism. Nyob rau hauv xyoo tas los no, nrog rau kev txhim kho sai ntawm cov thev naus laus zis xws li molecular biology thiab biochemistry, kev nce qib tseem ceeb tau ua tiav hauv kev tshawb fawb ntawm bioactive peptides.
Cov kws tshawb fawb tuaj yeem sib xyaw ua ke, cais, thiab txheeb xyuas ntau yam peptides, thiab tshawb nrhiav lawv cov txheej txheem ntawm kev ua tiav. Ntau qhov kev tshawb fawb tau pom tias peptides nrog cov qauv sib txawv muaj ntau hom kev ua haujlwm lom neeg, ua rau muaj kev cia siab tshiab rau kev daws teeb meem kev noj qab haus huv thiab kab mob. Hauv cov txheej txheem no, cov kws tshawb fawb tau kawm ntau tus peptides los ntawm txoj hauv kev xws li kev tshuaj ntsuam xyuas siab thiab kev ua haujlwm pov thawj. Vilon, raws li dipeptide nrog cov qauv tshwj xeeb thiab muaj peev xwm ua haujlwm, tau maj mam nkag mus rau hauv tib neeg txoj kev pom.
3. Kev tshawb nrhiav qhov tob ntawm cov txheej txheem ntawm cov ntaub so ntswg kho thiab rov tsim dua tshiab
Kev kho cov ntaub so ntswg thiab rov tsim dua tshiab yog cov txheej txheem tseem ceeb rau kev tswj hwm lub cev lub cev ua haujlwm ntawm cov kab mob thiab teb rau kev raug mob. Txawm li cas los xij, tseem muaj ntau qhov tsis sib xws hauv kev nkag siab tam sim no ntawm cov txheej txheem no, thiab yuav ua li cas los txhawb kev kho cov ntaub so ntswg kom zoo thiab rov tsim dua tshiab tau dhau los ua qhov tseem ceeb ntawm kev tshawb fawb. Stem cells ua lub luag haujlwm tseem ceeb hauv kev kho cov ntaub so ntswg thiab rov tsim dua tshiab, thiab kev tshawb fawb txog lawv cov kev ua kom muaj zog, kev loj hlob, thiab kev sib txawv ntawm cov txheej txheem tau txais kev mloog ntau.
Tsis tas li ntawd, yam xws li kev sib txuas thiab kev puas tsuaj ntawm extracellular matrix thiab intercellular signaling kuj tseem muaj feem cuam tshuam rau kev kho cov ntaub so ntswg. Hauv kev tshawb nrhiav cov txheej txheem ntawm kev kho cov ntaub so ntswg thiab rov tsim dua tshiab, cov kws tshawb fawb tau pom tias qee qhov peptides tuaj yeem tswj cov txheej txheem tseem ceeb no. Raws li qhov no, kev tshawb fawb ntawm peptides nrog lub peev xwm los tswj cov ntaub so ntswg kho thiab rov tsim dua tshiab tau txuas ntxiv mus. Vilon tau dhau los ua ib qho kev tshawb fawb tseem ceeb hauv daim teb no vim nws muaj peev xwm cuam tshuam rau kev txhawb nqa qia cell ua kom muaj zog thiab kev loj hlob thiab tswj kev ua haujlwm fibroblast.
Yuav ua li cas yog lub mechanism ntawm kev txiav txim ntawm Vilon?
1. Qhov cuam tshuam ntawm Chromosomes thiab Gene Expression
Txhawb kev Unwinding ntawm Heterochromatin: Cov kev tshawb fawb tau pom tias Vilon tuaj yeem ua rau tsis muaj zog (deheterochromatization) ntawm tag nrho cov heterochromatin nyob rau hauv cov kab lis kev cai lymphocytes ntawm cov neeg laus [1] . Qhov no txhais tau hais tias nws tuaj yeem qhib cov txheej txheem synthesis ntawm ribosomal genes tshwm sim los ntawm deheterochromatization ntawm nucleolar organizer cheeb tsam thiab tso cov noob uas yog inhibited vim lub condensation ntawm euchromatin cheeb tsam los tsim facultative heterochromatin. Nyob rau tib lub sij hawm, Vilon tsis ua rau lub unwinding ntawm lub constitutive heterochromatin nyob ib ncig ntawm lub centromere. Cov txiaj ntsig tau pom tias Vilon yuav maj mam qhib cov lus qhia heterochromatin (deheterochromatization) nrog hnub nyoog [1].
2. Lub luag haujlwm ntawm Kev Kho Mob
Kev Kho Kom Zoo rau Cov Neeg Mob Cancer: Hauv kev kho mob rau cov neeg mob qog noj ntshav laus, Vilon tau suav nrog hauv txoj kev kho mob raws li immunomodulator. Cov txiaj ntsig kev tshawb fawb ua ntej qhia tau hais tias daim ntawv thov Vilon tuaj yeem txhim kho 2-xyoo kev ciaj sia ntawm cov neeg mob, tiv thaiv cov teeb meem tom qab phais, qhov teeb meem nyob deb, rov tshwm sim, thiab qog nqaij hlav, thiab txhim kho lub neej zoo tom qab kev kho mob [2].
Antitumor Effects: Hauv kev sim tshuaj vitro, Vilon muaj cov tshuaj tiv thaiv kab mob rau kev loj hlob ntawm peb hom qog hlwb, uas yog tib neeg mob qog noj ntshav LOVO, tib neeg mob plab hnyuv MKN45, thiab tib neeg lub siab mob qog noj ntshav QGY7703, tab sis tsis muaj qhov pom tseeb inhibitory cuam tshuam rau tib neeg cov qe ntshav dawb [3] . Hauv vivo qog inhibition thwmsim qhia tias Vilon muaj cov nyhuv inhibitory ntawm kev loj hlob ntawm nas mob qog noj ntshav H22, thiab cov koob tshuaj zoo yog 15mg・Kg-1. Thaum siv cov koob tshuaj siab ntawm 30mg・Kg-1, cov qog inhibition ntawm cov qog nqaij hlav qog nqaij hlav H22 hauv cov nas nce mus txog ntau dua 60% [3].
Cov txiaj ntsig ntawm Cov Neeg Mob Ntshav Qab Zib: Hauv cov neeg laus hom I ntshav qab zib mellitus, Vilon, ua ib feem ntawm kev kho mob dav dav, tuaj yeem txhim kho cov coagulation thiab hemostasis muaj nuj nqi, tshwm sim los ntawm kev nce hauv cov ntsiab lus ntawm ntuj anticoagulants (antithrombin III thiab protein C) thiab stimulation ntawm fibrinolysis [4] . Nyob rau tib lub sijhawm, feem ntau, Vilon tuaj yeem txo cov koob tshuaj insulin uas yuav tsum tau ua kom ruaj khov carbohydrate metabolism. Tsis tas li ntawd, nws tseem tuaj yeem txo cov ntsiab lus ntawm T pab hlwb, T-dependent thiab tsis-T-nyob ntawm NK hlwb, normalize cov theem ntawm cov nquag T lymphocytes, B lymphocytes, thiab IgA, thiab muaj ib tug stabilizing nyhuv ntawm lub cev thiab hemostasis muaj nuj nqi [4].
3. Qhov cuam tshuam rau ntawm tes thiab lub cev ua haujlwm
Stimulating Hepatocyte Regeneration: Nyob rau hauv cov qauv nas ntawm daim siab mob siab vim yog cov pa roj carbon tetrachloride lom, Vilon muaj qee yam cuam tshuam rau kev rov ua haujlwm ntawm hepatocytes thiab kev tsim tawm ntawm daim siab hauv cov nas nrog daim siab cirrhosis. Ob lub lis piam tom qab siv cov tshuaj, cov kev ua ntawm qabzib-6-phosphatase (G6P) nyob rau hauv daim siab ntawm nas nrog daim siab cirrhosis txo, thiab Vilon tuaj yeem nce nws. Hauv cov nas tsis kho, cov ntsiab lus ntawm tag nrho glycogen thiab nws cov khoom thiab cov haujlwm ntawm G6P tseem nyob rau theem ua ntej cirrhosis. Thoob plaws hauv qhov kev sim, cov dej num ntawm glycogen phosphorylase (GP) thiab glycogen synthase (GS) nyob rau hauv daim siab ntawm nas nrog daim siab cirrhosis nyob rau hauv ob pawg tsis muaj qhov sib txawv ntawm kev tswj hwm qhov tseem ceeb. Vilon muaj qhov tsis muaj zog stimulating nyhuv ntawm kev tsim ntawm daim siab nyob rau hauv nas nrog daim siab cirrhosis, manifested raws li tag nrho cov protein cov ntsiab lus thiab ploidy theem nyob rau hauv lub hepatocytes ntawm pab pawg neeg thib ob ntawm nas yog 4.7% thiab 11.5% siab dua cov thawj pab pawg neeg feem [5].
Cov teebmeem ntawm Radiosensitive Organs: Vilon nkoos proliferative kev ua ntawm thymocytes thiab txhim khu lub proliferative muaj peev xwm ntawm txoj hnyuv kab mob, li no stimulating lub post-radiation rov qab ntawm lub cev tseem ceeb. Piv txwv li, kev tshawb fawb ntawm cov nas thiab nas uas tsis muaj zog uas tau txais ib lub cev tag nrho lub cev gamma-ray irradiation (6Gy) tau pom tias Vilon muaj kev cuam tshuam rau kev ua haujlwm morphology ntawm thymus, spleen, thiab duodenum [6].
4. Lub luag haujlwm ntawm qib Molecular
Ua tau Mechanism of Binding to the Membrane: Molecular dynamics trajectory kev tshawb fawb tau pom tias ob qho tib si Vilon (lysylglutamic acid dipeptide) thiab thymopoietin (Glu-Trp) muaj intramolecular ntsev txuas rau hauv lawv cov qauv, txo lawv cov conformational yooj. Vim lub aliphatic sab saw ntawm Lys, Vilon yog qhov hloov tau yooj yim dua. Ib qho ua tau rau lub ligand-receptor binding ntawm dipeptide rau lub excitable daim nyias nyias tau raug npaj, uas yog, khi los ntawm nitrogen thiab oxygen atoms uas tsim cov ntsev choj [7].
Cov teebmeem ntawm Protein Expression nyob rau hauv Nucleolar Organizing Cheeb Tsam: Vilon txhawb thiab inhibits qhov kev qhia ntawm AIDS proteins nyob rau hauv lub nucleolar koom cheeb tsam ntawm cov ntshav cov ntaub so ntswg thiab epithelial cell nuclei, ntsig txog tsim los yog txo cov tsim, sib sau ua ke, thiab thauj ribosomes mus rau lub cytoplasm, yog li txiav txim siab lub synthesis ntawm cov protein. Tsis tas li ntawd, peptide no tseem txhawb kev hloov pauv ntawm thymocytes rau hauv cov hlwb tawg [8].
Cov ntawv thov Vilon yog dab tsi?
1. Anti-aging
Vilon yog suav tias yog lub peev xwm los tiv thaiv kev laus peptide uas tuaj yeem ncua sijhawm cellular senescence los ntawm kev txhim kho plab hnyuv, txhim kho kev ua haujlwm enzyme, thiab txhawb kev nqus cov as-ham. Tsis tas li ntawd, Vilon kuj tau pom tias tuaj yeem txhim kho kev qhia ntawm daim tawv nqaij collagen thiab txo cov cim ntawm daim tawv nqaij laus. Cov kev tshawb fawb tau pom tias peptide KE (Lys-Glu, Vilon) tuaj yeem nce qhov kev nthuav qhia ntawm collagen 1 hauv kab lis kev cai ntawm senescent tawv nqaij fibroblasts los ntawm 83%; nws kuj nce qhov kev nthuav qhia thaj tsam ntawm sirtuin 6 hauv kab lis kev cai ntawm cov tub ntxhais hluas thiab laus ntawm daim tawv nqaij fibroblasts los ntawm 1.6 thiab 2.6 npaug ntawm [11].
2. Cov ntaub so ntswg kho thiab rov tsim dua tshiab
Vilon qhia txog lub peev xwm tseem ceeb hauv kev kho cov ntaub so ntswg thiab rov tsim dua tshiab. Nws tuaj yeem pab txhawb kev ua kom thiab loj hlob ntawm qia hlwb, ua kom lub qhov txhab zoo thiab kho cov ntaub so ntswg. Cov kev tshawb fawb tseem pom tau tias Vilon tuaj yeem txhawb kev kho ntawm daim tawv nqaij, plab hnyuv, thiab lwm yam ntaub so ntswg los ntawm kev tswj cov haujlwm fibroblast. Cov kev tshawb fawb tau pom tias cov hluavtaws dipeptide Vilon tau ntxiv rau cov kab lis kev cai nruab nrab ntawm cov nas ntawm cov hnub nyoog sib txawv. Cov txiaj ntsig tau pom tias Vilon tuaj yeem ua rau cov ntaub so ntswg morphological ruaj khov, ua kom rov tsim dua tshiab thiab ua haujlwm ntawm cov hlwb, thiab muaj kev cuam tshuam zoo dua rau kev cog cov nas laus, qhia tias Vilon muaj peev xwm kho cov ntaub so ntswg [9].
3. Kev tiv thaiv kab mob plawv thiab lub raum
Vilon tseem muaj txiaj ntsig zoo rau lub plawv thiab lub raum noj qab haus huv. Nws tuaj yeem txhim kho cov hlab plawv ua haujlwm los ntawm kev hloov pauv cov qauv qhia cov noob thiab txhim kho microvascular permeability hauv cov kab mob raum, txhawb kev ua kom zoo ntawm cov ntshav coagulation.
4. Kev siv hauv kev kho mob qog noj ntshav
Kev Kho Kom Zoo rau Cov Neeg Laus Cancer: Hauv kev kho mob rau cov neeg laus mob qog noj ntshav, Vilon tau suav nrog hauv txoj kev kho mob raws li immunomodulator. Piv txwv li, nyob rau hauv kev kho mob ntawm cov neeg laus cov neeg mob uas muaj mob cancer ntawm lub qhov quav thiab txoj hnyuv, cov kev tshawb fawb ua ntej qhia tau hais tias daim ntawv thov ntawm Vilon tuaj yeem txhim kho cov neeg mob 2 xyoos, tiv thaiv cov teeb meem tom qab phais, qhov teeb meem nyob deb, rov tshwm sim, thiab qog nqaij hlav [2].
Patient Stratification for Multiple Cancers: Qhov Variation of information fused Layers of Networks algorithm (ViLoN) saws los ntawm Vilon yog ib tug tshiab network-raws li txoj kev uas yuav siv tau los integrate ntau molecular maps. Nyob rau hauv cov nqe lus ntawm tus neeg mob stratification, txoj kev no tau raug txheeb xyuas ntawm ntau yam kev sib txuas ntawm cov ntaub ntawv hom (gene expression, methylation, copy number), thiab muaj kev txhim kho tseem ceeb ntawm tus neeg mob stratification, thiab muaj kev sib tw zoo sib xws hauv txhua qhov xwm txheej. Hauv cov pawg me me (qhov quav adenocarcinoma: 90 tus neeg mob, mob qog noj ntshav: 180 tus neeg), suav nrog kev paub txog kev ua haujlwm ua ntej (KEGG, GO) yog qhov tseem ceeb rau kev ua tiav cov txiaj ntsig zoo [10].
5. Kev siv hauv kev kho mob siab
Kev cuam tshuam rau daim siab ntawm nas nrog daim siab ua haujlwm siab: Cov teebmeem ntawm kev npaj dipeptide 'Vilon' rau kev rov ua haujlwm ntawm hepatocytes thiab kev tsim tawm ntawm daim siab hauv cov nas nrog daim siab cirrhosis tau kawm. Cov nas uas muaj daim siab mob siab vim yog cov pa roj carbon tetrachloride lom rau 4 lub hlis tau muab Vilon (1.7 micrograms / kg) thiab txhaj tshuaj txhua hnub rau 5 hnub. Cov txiaj ntsig tau pom tias ob lub lis piam tom qab siv cov tshuaj, kev ua haujlwm ntawm cov piam thaj-6-phosphatase (G6P), uas tau txo qis los ntawm 1.2 npaug ntawm daim siab mob siab, nce ntxiv raws li kev ua ntawm Vilon. Vilon muaj qhov tsis muaj zog stimulating nyhuv ntawm kev tsim ntawm daim siab nyob rau hauv nas nrog daim siab cirrhosis, manifested raws li tag nrho cov protein cov ntsiab lus thiab ploidy theem nyob rau hauv lub hepatocytes ntawm pab pawg neeg thib ob ntawm nas yog 4.7% thiab 11.5% siab dua cov thawj pab pawg neeg feem [5].
6. Cov ntawv thov hauv Kev Kho Mob Ntshav Qab Zib
Kev cuam tshuam rau cov neeg laus mob ntshav qab zib mellitus: Vilon, raws li thymic mimetic, yog siv los ua cov tshuaj adjuvant hauv kev kho mob ntawm cov neeg laus mob ntshav qab zib hom I. Cov txiaj ntsig tau pom tias daim ntawv thov Vilon optimizes coagulation thiab hemostasis muaj nuj nqi, manifested raws li ib tug nce nyob rau hauv cov ntsiab lus ntawm ntuj anticoagulants (antithrombin III thiab protein C) thiab stimulation ntawm fibrinolysis. Feem ntau, Vilon txo cov koob tshuaj insulin uas yuav tsum tau ua kom ruaj khov carbohydrate metabolism. Nyob rau tib lub sijhawm, Vilon kuj txo cov ntsiab lus ntawm T pab hlwb, T-dependent thiab tsis-T-nyob ntawm NK hlwb, thiab normalizes cov qib ntawm cov nquag T lymphocytes, B lymphocytes, thiab IgA, qhia tias Vilon muaj ib tug stabilizing nyhuv ntawm lub cev thiab hemostasis muaj nuj nqi [4].
7. Kev siv hauv kev kho mob ntawm hluav taws xob raug mob
Cov teebmeem ntawm Radiosensitive Organs: Cov teebmeem ntawm vilon thiab epithalon ntawm kev ua haujlwm morphology ntawm thymus, spleen, thiab duodenum ntawm cov nas tsis zoo thiab cov nas uas tau txais ib lub cev tag nrho γ-ray irradiation ntawm 6Gy tau kawm. Cov txiaj ntsig tau pom tias vilon txhawb nqa kev ua haujlwm loj ntawm thymocytes thiab txhim kho lub peev xwm loj hlob ntawm cov kab mob hauv plab hnyuv, yog li txhawb kev rov qab hluav taws xob rov qab ntawm cov kabmob tseem ceeb [6].
Hauv kev xaus, raws li ib qho tshwj xeeb dipeptide, Vilon tuaj yeem txhim kho txoj hnyuv ua haujlwm, ncua kev laus, pab kho cov ntaub so ntswg thiab rov tsim dua tshiab, thiab tswj cov hlab plawv thiab lub raum noj qab haus huv. Nws kuj muaj txiaj ntsig zoo hauv kev kho mob qog noj ntshav, kab mob siab, ntshav qab zib, thiab hluav taws xob raug mob.
Hais Txog Tus Sau
Cov ntaub ntawv hais saum toj no yog txhua yam tshawb fawb, kho thiab sau los ntawm Cocer Peptides.
Scientific Journal Author Kańduła MM yog tus kws tshawb fawb nrog kev koom tes rau ntau lub koom haum muaj koob npe, suav nrog Janssen Pharmaceuticals, BOKU University, BOKU Vienna, Boston University, thiab Johannes Kepler University Linz. Nws txoj kev tshawb fawb nthuav dav ntau thaj chaw, qhia txog nws txoj kev txawj ntse. Hauv Biochemistry & Molecular Biology, nws tau pab txhawb kev nkag siab txog cov txheej txheem ntawm tes thiab kev sib cuam tshuam ntawm molecular.
Nws txoj haujlwm hauv Cell Biology suav nrog kev kawm txog cov qauv thiab kev ua haujlwm ntawm cov hlwb, uas yog qhov tseem ceeb rau kev tsim cov tswv yim kho tshiab. Hauv Biotechnology & Applied Microbiology, Kańduła MM tau tshawb nrhiav txoj hauv kev tshiab rau kev siv cov kab mob microbial los daws cov teeb meem siv tau. Nws txoj kev tshawb fawb hauv Life Sciences & Biomedicine - Lwm Cov Ntsiab Lus qhia txog nws txoj kev koom tes nrog cov txheej txheem txiav thiab thev naus laus zis uas dhau los ua ib thaj tsam ntawm kev tshawb fawb. Tsis tas li ntawd, nws txoj haujlwm hauv Engineering qhia nws lub peev xwm los siv cov qauv kev tshawb fawb rau kev siv tswv yim. Los ntawm nws txoj kev tshawb fawb ntau yam, Kańduła MM tau ua rau muaj txiaj ntsig zoo rau lub zej zog kev tshawb fawb, cuam tshuam rau ob qho tib si theoretical kev nce qib thiab kev siv tswv yim hauv tshuaj thiab biology. Kańduła MM tau teev nyob rau hauv qhov kev siv ntawm cov ntaub ntawv pov thawj [10].
