Research Ubushakashatsi bwa Vilon
Ni ubuhe bushakashatsi bwakozwe na Vilon?
1. Birakenewe byihutirwa ubushakashatsi kubusaza n'indwara
Hamwe nihuta ryibikorwa byogusaza kwabaturage kwisi yose, urukurikirane rwibibazo bijyanye no gusaza, nka senescence selile, kugabanuka kwimikorere yimitsi, hamwe n’indwara nyinshi z’indwara zikuze, byabaye ibibazo byihutirwa by’ubuvuzi bigomba gukemurwa. Senescence ya selile iherekejwe no gutakaza buhoro buhoro ubushobozi bwo kubungabunga homeostasis no gusana ingirangingo zangiritse, bityo bikaba ngombwa cyane kubona ibintu bishobora gutinza inzira ya senescence ya selile no kongera imikorere yo gusana ingirangingo.
Muri icyo gihe, indwara zikomeye nka kanseri, diyabete, indwara z'umutima n'imitsi, n'indwara z'umwijima zibangamira cyane ubuzima bw'abantu. Uburyo gakondo bwo kuvura bufite aho bugarukira mu guhangana nizi ndwara, kandi hakenewe byihutirwa gushyiraho uburyo bushya bwo kuvura nibiyobyabwenge. Kurugero, abarwayi ba kanseri bageze mu zabukuru ntibihanganira radiotherapi na chimiotherapie, kandi ingaruka zo kuvura ntabwo zishimishije. Birihutirwa gushakisha uburyo bwo kuvura bwizewe kandi bwiza. Mu bihe bigoye, abahanga mu bya siyansi berekeje ibitekerezo byabo kuri peptide ya bioactive, bizeye ko izabona intambwe ishimishije, maze ubushakashatsi kuri Vilon bubaho.
2. Iterambere rikomeye ryubushakashatsi kuri Bioactive Peptide
Ibintu bya peptide bibaho cyane mubinyabuzima bizima kandi bigira uruhare mubikorwa byinshi byingenzi byumubiri, nkibimenyetso byerekana ingirabuzimafatizo, kugenzura ubudahangarwa bw'umubiri, no kugenzura metabolike. Mu myaka yashize, hamwe niterambere ryihuse ryikoranabuhanga nka biyolojiya y’ibinyabuzima na biohimiya, hari intambwe igaragara yatewe mu bushakashatsi kuri peptide ya bioactive.
Abahanga barashobora guhuza neza, gutandukanya, no kumenya peptide zitandukanye, kandi bagashakisha byimazeyo uburyo bwabo bwo gukora. Umubare munini wubushakashatsi bwerekanye ko peptide ifite imiterere itandukanye ifite ibikorwa bitandukanye byibinyabuzima, bizana ibyiringiro bishya byo gukemura ibibazo byubuzima nindwara. Muri iki gikorwa, abashakashatsi bakoze ubushakashatsi ku mubare munini wa peptide binyuze mu buryo nko gusuzuma cyane-kugenzura no kugenzura imikorere. Vilon, nka dipeptide ifite imiterere yihariye nimirimo ishobora gukora, yagiye buhoro buhoro mubitekerezo byabantu.
3. Ubushakashatsi bwimbitse bwuburyo bwo gusana imyenda no kuvugurura
Gusana imyenda no kuvugurura ni inzira zingenzi zo kubungabunga imikorere isanzwe yumubiri wibinyabuzima no gusubiza ibikomere. Nubwo bimeze bityo ariko, haracyariho icyuho kinini mubisobanuro byubu byerekeranye niyi nzira, nuburyo bwo guteza imbere imikorere myiza yo gusana no kuvugurura ibintu byabaye intumbero yubushakashatsi. Ingirabuzimafatizo zigira uruhare runini mu gusana no kuvugurura ingirabuzima fatizo, kandi ubushakashatsi ku mikorere yabyo, gukwirakwizwa, hamwe n’uburyo butandukanye bwitabiriwe cyane.
Byongeye kandi, ibintu nka synthesis hamwe no gutesha agaciro matrixe idasanzwe na signal intercellular selile nabyo bigira ingaruka zikomeye mugusana ingirangingo. Muri gahunda yo gucukumbura uburyo bwo gusana no kuvugurura ingirabuzimafatizo, abashakashatsi basanze peptide zimwe na zimwe zishobora kugenzura izi nzira zingenzi. Hashingiwe kuri ibi, ubushakashatsi kuri peptide bufite ubushobozi bwo kugenzura imikorere no gusana ingirabuzima fatizo bwakomeje kwiyongera. Vilon yabaye ikintu cyingenzi cyubushakashatsi muriki gice kubera ingaruka zishobora gutera mugushigikira ingirabuzimafatizo no gukwirakwiza no kugenzura ibikorwa bya fibroblast.
Ni ubuhe buryo bwo gukora bwa Vilon?
1. Ingaruka kuri Chromosomes no Kugaragaza Gene
Guteza imbere Kudashaka kwa Heterochromatine: Ubushakashatsi bwerekanye ko Vilon ishobora gutera ubushake (deheterochromatisation) ya heterochromatine yuzuye muri lymphocytes zifite umuco zabasaza [1] . Ibi bivuze ko ishobora gukora synthesis ya genes ya ribosomal iterwa na deheterochromatisation yakarere ka nucleolar itegura kandi ikarekura genes zabujijwe kubera guhuza akarere ka euchromatine kugirango habeho heterochromatine. Mugihe kimwe, Vilon ntabwo itera kudashaka kwa heterochromatine igizwe na centromere. Ibisubizo byerekana ko Vilon izagenda ikora buhoro buhoro imikorere ya heterochromatine (deheterochromatisation) hamwe n'imyaka [1].
2. Uruhare mu kuvura indwara
Ubuvuzi Bwuzuye ku barwayi ba Kanseri: Mu kuvura abarwayi ba kanseri bageze mu zabukuru, Vilon ashyirwa muri gahunda yo kuvura nka immunomodulator. Ibyavuye mu bushakashatsi bwakozwe byerekana ko ishyirwa mu bikorwa rya Vilon rishobora kuzamura imyaka 2 y’ubuzima bw’abarwayi, bikarinda ingorane nyuma yo kubagwa, ingorane za kure, kongera kubaho, ndetse n’ibibyimba bikwirakwira, kandi bikazamura imibereho nyuma yo kuvurwa neza [2].
Ingaruka ya Antitumor: Mu bushakashatsi bwa vitro, Vilon igira ingaruka ziterwa no gukumira ikura ryubwoko butatu bwingirangingo yibibyimba, aribyo kanseri yibara yumuntu LOVO, kanseri yigifu yumuntu MKN45, na kanseri yumwijima yumuntu QGY7703, ariko nta ngaruka zigaragara zibuza ingirabuzimafatizo zamaraso zisanzwe zabantu [3] . Mu bushakashatsi bwa vivo ibibyimba byerekana ko Vilon igira ingaruka mbi ku mikurire ya kanseri yumwijima yimbeba H22, kandi ikinini cyiza ni 15mg ・ Kg-1. Iyo hakoreshejwe urugero rwinshi rwa 30mg ・ Kg-1, igipimo cyo kubuza ibibyimba kanseri y'umwijima yatewe kanseri y'umwijima H22 mu mbeba igera kuri 60% [3].
Ingaruka ku barwayi ba diyabete: Mu barwayi bageze mu zabukuru abarwayi ba diyabete yo mu bwoko bwa I, Vilon, mu rwego rwo kuvura byimazeyo, irashobora guhindura imikorere ya coagulation na hemostasis, igaragara nko kwiyongera kw'ibirimo anticagulants (antithrombine III na proteyine C) no gukurura fibrinolysis [4] . Muri icyo gihe, mu bihe byinshi, Vilon irashobora kandi kugabanya urugero rwa insuline ikenerwa kugirango ihindure metabolisme ya karubone. Byongeye kandi, irashobora kandi kugabanya ibiri muri selile zifasha T, T-iterwa na T na selile N-selile, igahindura urwego rwa lymphocytes T ikora, lymphocytes B, na IgA, kandi ikagira ingaruka zihamye kumikorere yumubiri no mumikorere ya hemostasis [4].
3. Ingaruka ku mikorere y'utugari n'inzego
Kubyutsa Hepatocyte Kuvugurura: Muburyo bwimbeba ya cirrhose yumwijima iterwa nuburozi bwa karubone tetrachloride, Vilon igira uruhare runini mukugarura ibikorwa byimikorere ya hepatocytes no kuvugurura umwijima mumbeba hamwe na cirrhose yumwijima. Ibyumweru bibiri nyuma yo gukoresha ibiyobyabwenge, ibikorwa bya glucose-6-fosifata (G6P) mu mwijima wimbeba hamwe na cirrhose yumwijima bigabanuka, kandi Vilon irashobora kwiyongera. Imbeba zitavuwe, ibirimo glycogene yose hamwe nibiyigize hamwe nibikorwa bya G6P biracyari kurwego rwa mbere ya cirrhose. Mu bushakashatsi bwose, ibikorwa bya glycogene fosifora (GP) na synthase ya glycogene (GS) mu mwijima wimbeba hamwe na cirrhose yumwijima mumatsinda yombi ntaho bitandukaniye nagaciro kayobora. Vilon ifite imbaraga nke zitera imbaraga zo kuvugurura umwijima mu mbeba hamwe na cirrhose yumwijima, bigaragarira nkibintu byose bya poroteyine hamwe n’urwego rwa ploidy muri hepatocytes yo mu itsinda rya kabiri ry’imbeba zingana na 4.7% na 11.5% ugereranije n'iz'itsinda rya mbere [5].
Ingaruka ku nzego za Radiyo Yumva: Vilon itera ibikorwa byinshi bya thymocytes kandi ikongerera imbaraga zo gukwirakwira kwingirangingo zo munda, bityo bigatera imbaraga nyuma yimirasire yingingo zingenzi. Kurugero, ubushakashatsi bwakozwe ku mbeba n'imbeba byakiriye umubiri wose wa gamma-ray irrasiyo (6Gy) byerekanye ko Vilon igira ingaruka kumikorere ya thymus, spleen, na duodenum [6].
4. Inshingano kurwego rwa Molecular
Uburyo bushoboka bwo guhuza Membrane: Ubushakashatsi bwakozwe na molekulari dinamike bwerekanye ko Vilon (lysylglutamic acide dipeptide) na thymopoietin (Glu-Trp) irimo ibiraro byumunyu mwinshi muburyo bwabo, bikagabanya guhinduka kwabyo. Bitewe numurongo wa alifatique kuruhande rwa Lys, Vilon iroroshye guhinduka. Hatanzwe uburyo bushoboka bwo guhuza ligand-reseptor ihuza dipeptide na membrane ishimishije, ni ukuvuga guhuza binyuze muri atome ya azote na ogisijeni bigize ikiraro cyumunyu [7].
Ingaruka ku mvugo ya poroteyine mu karere gategura Nucleolar: Vilon itera kandi ikabuza kwerekana poroteyine za sida mu turere twa nucleolar zitunganya uturemangingo twa serumu na epithelial selile nuclei, bikora cyangwa bigabanya imiterere, guteranya, no gutwara ribosomes muri cytoplazme, bityo bikagaragaza ubukana bwa sintezamubiri ya poroteyine muri selile. Byongeye kandi, iyi peptide iteza imbere guhindura thymocytes mu ngirabuzimafatizo zigwira [8].
Ni ubuhe buryo bukoreshwa na Vilon?
1. Kurwanya gusaza
Vilon ifatwa nka peptide ishobora kurwanya gusaza ishobora gutinza ingirabuzimafatizo mu kunoza imikorere ya gastrointestinal, kongera ibikorwa bya enzyme, no guteza imbere intungamubiri. Byongeye kandi, Vilon yasanze kandi ibasha kunoza imvugo ya kolagen y'uruhu no kugabanya ibimenyetso byo gusaza k'uruhu. Ubushakashatsi bwerekanye ko peptide KE (Lys-Glu, Vilon) ishobora kongera imiterere ya kolagen 1 mu muco wa fibroblast y'uruhu rwa senescent ku kigero cya 83%; byongera kandi imiterere ya sirtuin 6 mumico ya fibroblast y'uruhu ruto kandi ishaje inshuro 1,6 na 2,6 ..
2. Gusana imyenda no kuvugurura
Vilon yerekana ubushobozi bukomeye mugusana ingirabuzimafatizo no kuvugurura. Irashobora gushyigikira ibikorwa no gukwirakwiza ingirabuzimafatizo, kwihutisha gukira ibikomere no gusana ingirangingo. Ubushakashatsi bwerekanye kandi ko Vilon ishobora guteza imbere gusana uruhu, amara, nizindi ngingo muguhuza ibikorwa bya fibroblast. Ubushakashatsi bwerekanye ko dipeptide ya sintetike ya Vilon yongewe kumuco usobanutse kuva imbeba zo mumyaka itandukanye. Ibisubizo byerekanye ko Vilon ishobora gutera ingirabuzima fatizo zifatika, igahindura imikorere mishya yimikorere yingirabuzimafatizo, kandi ikagira ingaruka zikomeye kubushakashatsi bwimbeba zishaje, byerekana ko Vilon ifite ubushobozi bwo gusana ingirangingo [9].
3. Kurinda umutima nimiyoboro yumutima
Vilon igira kandi ingaruka nziza kubuzima bwumutima nimiyoboro yimpyiko. Irashobora kunoza imikorere yumutima nimiyoboro ihindura imiterere ya gene kandi ikongerera mikorobe mumitsi yindwara zimpyiko, igateza imbere guhuza amaraso.
4. Gusaba kuvura Kanseri
Umuti wuzuye kubarwayi ba kanseri bageze mu zabukuru: Mu kuvura abarwayi ba kanseri bageze mu zabukuru, Vilon ashyirwa muri gahunda yo kuvura nka immunomodulator. Kurugero, mubuvuzi bwuzuye bwabarwayi bageze mu zabukuru barwaye kanseri yinkondo yumura na kanseri yumura, ibisubizo byubushakashatsi bwibanze byerekana ko ishyirwa mu bikorwa rya Vilon rishobora kuzamura ubuzima bw’imyaka 2 y’abarwayi, bikarinda ingorane nyuma yo kubagwa, ingorane za kure, kongera kubaho, ndetse n’ibibyimba bikwirakwira [2].
Gutandukanya abarwayi kuri Kanseri nyinshi: Itandukaniro ryamakuru yahujwe Imirongo ya Networks algorithm (ViLoN) yemejwe na Vilon nuburyo bushya bushingiye kumurongo bushobora gukoreshwa muguhuza amakarita menshi ya molekile. Ku bijyanye no gutondekanya abarwayi, ubu buryo bwagenzuwe ku buryo butandukanye bwo guhuza amakuru (imvugo ya gene, methylation, nimero ya kopi), kandi bugira ingaruka zikomeye ku ntera y’abarwayi, kandi bufite irushanwa rihoraho muri byose. Mu matsinda mato (adenocarcinoma rectal: abantu 90, kanseri yo mu nda: indwara 180), gushyiramo ubumenyi bukora mbere (KEGG, GO) ni ngombwa kugirango umuntu agere ku musaruro mwiza [10].
5. Gusaba mu Kuvura Indwara Zumwijima
Ingaruka ku mwijima wimbeba hamwe na Cirrhose yumwijima: Hakozwe ubushakashatsi ku ngaruka zo gutegura dipeptide 'Vilon ' ku kugarura ibikorwa byimikorere ya hepatocytes no kuvugurura umwijima mu mbeba hamwe na cirrhose yumwijima. Imbeba zifite cirrhose yumwijima ziterwa nuburozi bwa karubone tetrachloride mumezi 4 zahawe Vilon (micrograms 1.7 / kg) hanyuma zitera buri munsi iminsi 5. Ibisubizo byagaragaje ko nyuma y'ibyumweru bibiri nyuma yo gukoresha ibiyobyabwenge, ibikorwa bya glucose-6-fosifata (G6P), byagabanutseho inshuro 1,2 muri cirrhose y'umwijima, byiyongereye ku gikorwa cya Vilon. Vilon ifite imbaraga nke zitera imbaraga zo kuvugurura umwijima mu mbeba hamwe na cirrhose yumwijima, bigaragarira nkibintu byose bya poroteyine hamwe n’urwego rwa ploidy muri hepatocytes yo mu itsinda rya kabiri ry’imbeba zingana na 4.7% na 11.5% ugereranije n'iz'itsinda rya mbere [5].
6. Gusaba kuvura Diyabete
Ingaruka ku barwayi ba diyabete bageze mu zabukuru: Vilon, nka mimetic thimic, ikoreshwa nk'umuti wongeyeho mu kuvura byimazeyo abarwayi ba diyabete yo mu bwoko bwa I. Ibisubizo byerekana ko ikoreshwa rya Vilon ritezimbere imikorere ya coagulation na hemostasis, bigaragarira nkubwiyongere bwibiri muri anticagulants karemano (antithrombine III na protein C) hamwe no gukurura fibrinolysis. Kenshi na kenshi, Vilon igabanya urugero rwa insuline ikenewe kugirango ihindure metabolisme. Muri icyo gihe, Vilon igabanya kandi ibikubiye mu ngirabuzimafatizo za T zifasha, T-iterwa na T na selile ya NK, kandi igahindura urwego rwa lymphocytes T ikora, lymphocytes B, na IgA, byerekana ko Vilon igira ingaruka zihamye ku mikorere y’umubiri ndetse n’imikorere ya hemostasis [4].
7. Gusaba mukuvura ibikomere byimirasire
Ingaruka ku nzego za Radiyo Yumva: Hakozwe ubushakashatsi ku ngaruka za vilon na epithalon kuri morphologie ikora ya thymus, spleen, na duodenum yimbeba nimbeba zakira umubiri wose γ-ray irrasi ya 6Gy. Ibisubizo byerekana ko vilon itera ibikorwa byinshi bya thymocytes kandi ikongerera imbaraga zo kwiyongera kwingirangingo zo mu mara, bityo bigatuma imirasire ikura nyuma yimirasire yingingo zingenzi [6].
Mu gusoza, nka dipeptide idasanzwe, Vilon irashobora kunoza imikorere ya gastrointestinal, gutinda gusaza, gufasha mugusana ingirangingo no kuvugurura, kandi ikagumana ubuzima bwumutima nimiyoboro yimpyiko. Ifite kandi ingaruka nziza mu kuvura kanseri, indwara z'umwijima, diyabete, n'imvune zikomoka ku mirasire.
Ibyerekeye Umwanditsi
Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.
Umwanditsi w'ikinyamakuru Scientific Kańduła MM ni umushakashatsi ufite aho ahuriye n'ibigo byinshi bizwi, birimo Janssen Pharmaceuticals, kaminuza ya BOKU, BOKU Vienne, kaminuza ya Boston, na kaminuza ya Johannes Kepler Linz. Ubushakashatsi bwe bukubiyemo ibice byinshi, byerekana ubuhanga bwe butandukanye. Muri Biochemie & Molecular Biology, yagize uruhare mu guteza imbere imyumvire yimikorere ya selile n'imikoranire ya molekile.
Ibikorwa bye muri Cell Biology bikubiyemo kwiga imiterere n'imikorere y'utugingo ngengabuzima, ari ngombwa mu gutegura ingamba nshya zo kuvura. Muri Biotechnology & Applied Microbiology, Kańduła MM yakoze ubushakashatsi ku buryo bushya bwo gukoresha sisitemu ya mikorobe kugirango ikemure ibibazo bifatika. Ubushakashatsi bwe muri Life Science & Biomedicine - Izindi ngingo zerekana uruhare rwe muburyo bugezweho hamwe nikoranabuhanga rirenga imipaka ya siyansi. Byongeye kandi, ibikorwa bye muri Engineering byerekana ubushobozi bwe bwo gukurikiza amahame ya siyansi mubikorwa bifatika. Binyuze mu bushakashatsi bwe butandukanye, Kańduła MM yagize uruhare runini mu bumenyi bwa siyansi, bigira uruhare mu iterambere ry’imyumvire ndetse no mu bikorwa bifatika mu buvuzi na biyolojiya. Kańduła MM yashyizwe ku rutonde rwerekana [10].
