Research Liva-g Ubushakashatsi
Ni ubuhe bushakashatsi bwakozwe na Liva-g?
The Free Radical Theory Theory: Mu myaka ya za 1950, Harman yatanze igitekerezo cy’ubusaza cy’ubusaza, avuga ko radicals yubuntu yakozwe mugihe cya metabolism selile yibasira biomolecules, bigatuma kwangirika kw ingirabuzimafatizo no gusaza. Iyi nyigisho yatanze ishingiro ryubushakashatsi bwibintu birwanya gusaza kandi bituma abahanga mu bya siyansi bashakisha ibintu bishobora gutesha agaciro radicals yubusa cyangwa kongera ubushobozi bwa antioxydeant ya selile.
Ivumburwa ryibintu bya peptide: Hamwe nubushakashatsi bwimbitse bwibinyabuzima, abahanga basanze ibintu bya peptide bigira uruhare runini mukumenyekanisha ingirabuzimafatizo, kugenzura metabolike, nibindi bintu. Bimwe mu bintu bya peptide bifite imirimo nka antioxydeant, anti-inflammatory, no guteza imbere gusana ingirabuzimafatizo, bitanga icyerekezo gishya cyo guteza imbere imiti mishya irwanya gusaza.
Ubushakashatsi n'Iterambere rya Liva-g: Kuruhande rwibi, abahanga bo muburusiya bakoze Liva-g bashingiye kubushakashatsi bwabo kubintu bya peptide. Bakuyemo ibice bya bioactive peptide mubice byinyamanswa hanyuma amaherezo babona Liva-g nyuma yuruhererekane rwo gusuzuma no gukora neza. Ubushakashatsi bwerekanye ko Liva-g ishobora kugenga imikorere ya selile no kongera ubushobozi bwa antioxydeant ya selile, bityo igatinda gusaza kwa selile.
Ni ubuhe buryo bwo gukora bwa Liva-g?
1. Ingaruka kuri sisitemu yo kurya
Kugenga ibikorwa bya Enzymes zifungura: Ubushakashatsi bwerekanye ko Liva-g (Lys-Glu-Asp-Ala) ari peptide ya hydrolyzable idakomeye. Peptide hydrolase mu mara mato ntishobora hydrolyze Liva-g ku rugero ruto cyane [1] . Mugihe cya vitro, Liva-g irashobora kugabanya ibikorwa bya glycyl-L-leucine dipeptidase mu mara mato 50%. Nyuma yo gutanga umunwa Liva-g ku mbeba ibyumweru bibiri, ibikorwa byimisemburo yigifu mu nyamaswa zikiri nto byagabanutse, mugihe inyamaswa zishaje zariyongereye. Twabibutsa ko akenshi, ibikorwa bya enzyme yimbeba zishaje nyuma yo kwakira Liva-g byari hafi yurwego rwinyamaswa zikiri mumatsinda yo kugenzura [1].
2. Ingaruka kuri Enkephalin Gutesha Enzymes muri Serumu
Kubuza Enkephalin Enzymes Yangiza: Ingaruka za peptide bioregulators nshya Liva-g na Epi (Ala-Glu-Asp-Gly) kuri sisitemu ya endogenous opioid yakozwe cyane cyane ubushobozi bwabo bwo guhindura imikorere yimisemburo ya enkephalin muri serumu no gukorana na reseptor ya opioid mubice byubwonko bwubwonko. Igikorwa cya enkephalinase cyagenwe muri vitro mugupima hydrolysis ya ⊃3; H-Leu-enkephalin imbere ya peptide yipimishije. Liva-g na Epi babujije imisemburo ya enkephalin itesha agaciro serumu yabantu. Ugereranije na peptidase izwi cyane nka puromycine, leupeptin, na D-PAM, Liva-g byagaragaye ko ikora neza. Ingaruka ya dose-inhibition ya Liva-g na Epi yarateguwe, kandi agaciro kabo ka IC₅₀ kari 20 na 500 μM [2, 3].
3. Ingaruka zo Kurinda Umwijima
Nkumukozi wa Hepatoprotective Agent: Gukoresha imiti myinshi mubasaza akenshi bitera umwijima gukora nabi. Kubwibyo, hamwe no kwiyongera kwimyaka, ibyago byo kwandura fibrotic umwijima, hepatite ikaze kandi idakira. Ninimpamvu yo gushakisha imiti mishya, ikora neza, kandi itagira ingaruka. Ubushakashatsi bumwe bwerekanye ko polypeptide ya hepatike (Ventvil) na tetrapeptide ya KEDA (Lys-Glu-Asp-Ala, Liva-g) ifite hepatoprotective, immunoprotective, hamwe no kurwanya gusaza. Mu nyamaswa no muri vitro yerekana ubushakashatsi bwindwara yumwijima (umwijima fibrotic induration, hepatite ikaze na karande), Ventvil na peptide ya KEDA byagaragaje imikorere myiza. Ventvil na peptide ya KEDA bigira ingaruka zihamye - guhuza ubudahangarwa bw'umubiri na antioxydeant no kugarura imikorere y'umwijima mugihe cya hepatite. Ubushakashatsi bwerekanye ko mugihe cyo gusaza, hasuzumwe ingaruka ntarengwa za hepatoprotective na immunoprotective ya peptide ..
4. Ingaruka kuri Chromatine ya Lymphocytes
Gukora Chromatine ya Lymphocytes: Hakozwe ubushakashatsi ku ngaruka za peptide ya peptide ya Liva-g ku bikorwa bya genoside ya ribosomal, ibipimo byo gutandukanya heterochromatine, polymorphism ya C heterochromatine yubatswe, hamwe n’imihindagurikire y’imiterere ya heterochromatine muri lymphocytes y’abasaza. Liva-g yatumye habaho gukora genoside ya ribosomal, depolymerisation ya heterochromatine igizwe na centromere, no kurekura genes zabujijwe kubera guhuza uturere twa euchromatine zijyanye n'imyaka muri chromosomes. Ibisubizo byerekanye ko Liva-g yatumye habaho deheterochromatisation (activation) ya chromatine muri chromosomes yabasaza, ibyo bikaba byagezweho muguhindura heterochromatine hamwe n’uturere twa heterochromatine muri chromosomes [5].
Ni ubuhe buryo bukoreshwa na Liva-g?
Ubushakashatsi ku Gusaza kwa Cellular
Liva-g irashobora guhindura ibikorwa byimisemburo yingenzi igira uruhare mu gusana ingirabuzimafatizo cyangwa inzira yo gusubiza ibibazo. Kurugero, ikorana nubugenzuzi bwa epigenetike kandi ikagira ingaruka kumihindagurikire yimiterere ya chromatine nuburyo bwo kwerekana imiterere ya gene, bityo bikagenga inzira yo gusaza kwa selile. Kwangirika kwa Oxidative kwiyegeranya mugihe kandi biganisha ku gusaza kwa selile bijyanye no kudakora neza. Ingaruka za Liva-g kumuhanda wa okiside itera ni imwe mubushakashatsi, kandi irashobora gutinza gusaza kwa selile muguhuza inzira zijyanye.
Ingaruka ku ndwara zumwijima
Hamwe no kwiyongera kwimyaka, gukoresha imiti myinshi nabasaza akenshi bitera imikorere mibi yumwijima, bityo bikongera ibyago byo kwandura fiboside yumwijima, hepatite ikaze na karande. Muri ibi bihe, ni ngombwa gushakisha imiti mishya, ikora neza, kandi itagira ingaruka. Ubushakashatsi bumwe bwerekanye ko hepatike polypeptide (Ventvil) hamwe na tetrapeptide ya KEDA (Lys-Glu-Asp-Ala, aribyo Liva-g) bifite hepatoprotective, immunoprotective, hamwe no kurwanya gusaza. Mubyitegererezo byinyamanswa byerekana umwijima (fibrosis yumwijima, hepatite ikaze na karande) no mubushakashatsi bwa vitro, Ventvil na peptide ya KEDA byagaragaje imikorere myiza. Ventvil na peptide ya KEDA bifite imbaraga zo guhuza imbaraga, zishobora guhindura ubudahangarwa bw'umubiri na antioxydeant no kugarura imikorere y'umwijima mugihe cya hepatite. Byongeye kandi, mugihe cyo gusaza, ingaruka za hepatoprotective na immunoprotective ziyi peptide zigera kuri byinshi [4].
Ingaruka kuri Genomic Parameter z'abarwayi ba Kanseri
Byagaragaye ko genome y’abarwayi barwaye kanseri y’amabere irangwa n’ubucucike bwinshi bw’imitsi imwe ya ADN ivunika, inshuro nyinshi zidasanzwe za chromosomal, hamwe n’ubwiyongere bwa chromatine. Gukoresha oligopeptide bioregulator Liva-g na cobalt ion nkibihindura bigira ingaruka zo kurinda imico ya lymphocyte yabarwayi barwaye kanseri yamabere, ishobora guhuza ibipimo byose byizwe. Ibi byerekana ko binyuze mu bushakashatsi bwakozwe na lymphocytes z'abarwayi barwaye kanseri y'ibere, hashobora gusuzumwa ingaruka zo kuvura abarwayi ba kanseri y'ibere ..
Ingaruka kuri Lymphocytes z'abarwayi bafite Hypertrophique Cardiomyopathie na Bene wabo
Ingaruka za peptide bioregulator Liva-g (Lys-Glu-Asp-Ala) yakoreshejwe yonyine kandi ifatanije na ion ya cobalt ku bikorwa by’uturere dutegura nucleolar (NORs) hamwe ninshuro yo guhuza chromosomes acrocentric muri lymphocytes z'abarwayi bafite hypertrophique cardiomyopathie na benewabo. Ibisubizo byerekanaga ko ibikorwa bya Liva-g na cobalt ion byongereye inshuro za NOR hamwe n amanota menshi ya 2 kubarwayi na benewabo. Izi nteruro kandi zagize uruhare runini mubikorwa byishyirahamwe rya chromosomes acrocentric, byagaragaye nkubwiyongere bukabije bwiki cyerekezo mumatsinda yombi yo kwiga.
Muri uru rubanza, ibikorwa bya Liva-g na cobalt ion byari byiza cyane. Kubera ko ibikorwa bya NORs hamwe ninshuro ziterwa na chromosomes ya acrocentric biterwa nubwiza bwimiterere yibihuru bya chromosomes acrocentric, hashobora kwemezwa ko Liva-g na cobalt ion bigira ingaruka kuri lymphocytes z'abarwayi bafite hypertrophique cardiomyopathie na benewabo, bigatuma chromatine ikomera. Ibi birashobora kuba ibisabwa kugirango irekurwa rya genes zidakorwa mugihe cyo guhuza abantu mubantu bagize itsinda ryubushakashatsi. Aya makuru atanga amakuru mashya ku ngaruka zo kurinda Liva-g na Liva-g + cobalt ion kuri lymphocytes y’abarwayi bafite hypertrophique cardiomyopathie na benewabo kandi bishobora kugira uruhare mu iterambere ry’uburyo bwo kuvura [7].
Ingaruka kuri Enkephalin Gutesha agaciro Enzymes muri Serumu
Hakozwe ubushakashatsi ku ngaruka nshya ya peptide bioregulators Liva-g (Lys-Glu-Asp-Ala) na Epi (Ala-Glu-Asp-Gly) kuri sisitemu ya endogenous opioid, cyane cyane ubushobozi bwabo bwo guhindura imikorere ya enzymes zangiza za enkephalin muri serumu no gukorana na reseptor ya opioide mu gice cya selile yubwonko. Mu bushakashatsi bwa vitro bwerekanye ibikorwa bya enkephalinase mu gupima igipimo cya hydrolysis ya ⊃3; H-Leu-enkephalin imbere ya Liva-g na Epi. Ibisubizo byerekanaga ko Liva-g na Epi babujije imisemburo ya enkephalin yangiza muri serumu yabantu. Liva-g yari nziza cyane kuruta zimwe mu nzitizi zizwi cyane za peptidase nka puromycine, leupeptin, na D-PAM. Ingaruka ya dose-inhibition umurongo wa Liva-g na Epi yarateguwe, kandi agaciro ka IC₅₀ kari 20 na 500 μM. Imikoranire hagati ya peptide na reseptor ya opioid yasuzumwe hifashishijwe uburyo bwa reseptor ya radioligand hamwe na [⊃3; H] [D-Ala⊃2;, D-Leu⁵] -enkephalin. Nta mikoranire yagaragaye hagati ya μ cyangwa δ opioide yakira mu gice cya membrane cyubwonko bwimbeba na peptide yipimisha [2, 3].
Mu gusoza, muguhindura imvugo ya gene no guteza imbere intungamubiri za poroteyine, Liva-g yerekana ingaruka zikomeye mukurwanya gusaza, immunomodulation, no kurinda imikorere yumwijima. Irashobora kugenga ibikorwa byimisemburo yigifu, ikabuza imisemburo ya enkephalin itesha agaciro, kandi igatinda gusaza kwa selile ikora imisemburo yingenzi kandi ikagira ingaruka kumihanda ya okiside. Byongeye kandi, Liva-g inatezimbere imikorere yumubiri hamwe na antioxydeant binyuze mu ngaruka zayo za hepatoprotective kandi igatera imbaraga zo gukira imikorere yumwijima mu ndwara nka hepatite. Ifite agaciro gakoreshwa mugukumira no kuvura indwara zifitanye isano kandi itanga icyerekezo gishya cyubushakashatsi bwo kurwanya gusaza no kubungabunga ubuzima.
Ibyerekeye Umwanditsi
Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.
Umwanditsi w'ikinyamakuru
Vladimir Khavinson yari umuhanga mu bumenyi bw’imyororokere w’Uburusiya akaba n'umwarimu uzwiho gukora ubushakashatsi bwa mbere mu binyabuzima byitwa peptide n’uruhare rwabo mu gutinda gusaza no guteza imbere ubuzima. Yabaye umuyobozi w'ikigo cya St. Petersburg Institute of Bioregulation na Gerontology kandi yari umunyamuryango uhuye n’ishuri ry’ubumenyi ry’Uburusiya. Ubushakashatsi bwe bwibanze ku guteza imbere imiti ishingiye kuri peptide kugira ngo yongere imikorere y’umubiri, irinde imbaraga za okiside, kandi itere kuramba. Mu myaka mirongo ine ishize, yakuyemo kandi ashushanya inganda nyinshi za peptide, bituma hashyirwaho imiti itandatu ya farumasi ishingiye kuri peptide hamwe n’ibindi 64 byongera ibiryo bya peptide mubikorwa byubuvuzi. Ibikorwa bya Khavinson byagize uruhare runini mu bijyanye na gerontologiya, cyane cyane mu gusobanukirwa uburyo bwo gusaza no guteza imbere ingamba zo kuzamura imibereho y’abakuze.
Vladimir Khavinson ni umuhanga mu bumenyi bw'ikirusiya akaba n'umwarimu w’Uburusiya, uzwi cyane ku bushakashatsi bukomeye yakoze kuri peptide bioregulator ndetse n'uruhare rwabo mu gukumira gusaza no kuzamura ubuzima. Yagize umwanya w'ubuyobozi mu kigo cya St. Petersburg Institute of Bioregulation na Gerontology kandi yari umunyamuryango uhuye n’ishuri ry’ubumenyi ry’Uburusiya. Ubushakashatsi bwe bwibanze ku guteza imbere imiti ishingiye kuri peptide kugira ngo yongere imikorere y’umubiri, irinde imbaraga za okiside, kandi yorohereze kuramba. Mu myaka irenga mirongo ine y’ubushakashatsi, yakuyemo kandi ashushanya ibintu bitandukanye bya peptide, byateje imbere imiti ivura imiti itandatu ishingiye kuri peptide n’ibindi 64 byongera peptide. Ibikorwa bya Khavinson byagize uruhare runini mu bijyanye na gerontologiya, cyane cyane mu gusobanukirwa uburyo bwo gusaza no guteza imbere ingamba zo kuzamura imibereho y’abasaza. Vladimir Khavinson yanditse ku rutonde rw'ibitekerezo [5].
