By Cocer Peptides 
Watanni 1 da suka gabata
DUKKANIN LABARI DA BAYANIN KYAUTATAWA DA AKE BAYAR A WANNAN SHAFIN KAWAI DOMIN WATSA BAYANI NE DA MANUFOFIN ILIMI.
Kayayyakin da aka bayar akan wannan gidan yanar gizon an yi niyya ne kawai don binciken in vitro. Binciken in vitro (Latin: *a cikin gilashi*, ma'ana a cikin gilashin gilashi) ana gudanar da shi a wajen jikin mutum. Waɗannan samfuran ba magunguna ba ne, Hukumar Abinci da Magunguna ta Amurka (FDA) ba ta amince da su ba, kuma dole ne a yi amfani da su don hanawa, magani, ko warkar da kowane yanayi na likita, cuta, ko cuta. Doka ta haramta shigar da waɗannan samfuran cikin jikin mutum ko dabba ta kowace hanya.
Dubawa
Osteoarthritis (OA) cuta ce ta haɗin gwiwa ta yau da kullun wacce ke da alaƙa da lalacewar guringuntsi, gyaran ƙashi na subchondral, da martanin kumburi na synovial, mai matukar cutar da ingancin rayuwar marasa lafiya. Tare da haɓakar tsufa na yawan jama'a, abubuwan da suka faru na OA suna karuwa, suna ɗaukar nauyi ga al'umma da iyalai.
Hoto 1 Kwatanta abubuwan da ke tattare da cututtukan cututtuka na rheumatoid arthritis da osteoarthritis. Rheumatoid amosanin gabbai yana da wani tsari mai kumburi wanda ke daidaitawa ta hanyar haɓakawa, daidaitawa, da amsawar autoimmune na stromal.
Lalacewar guringuntsi muhimmin mataki ne a ci gaban OA. Saboda ƙarancin gyare-gyaren kai na guringuntsi, lalacewa yana da wuyar warkewa ba tare da bata lokaci ba, yana haifar da ci gaba da cututtuka a hankali. Sabili da haka, gano hanyoyin da suka dace don gyaran lalacewar guringuntsi ya zama abin da aka mayar da hankali ga bincike a cikin maganin OA.
Cartalax sigar biopharmaceutical ce ta musamman da aka ƙera don haɓaka gyaran lalacewar guringuntsi. Ƙayyadadden abun da ke ciki ya bambanta dangane da bincike daban-daban da tsarin samarwa, amma yawanci yana ƙunshe da abubuwa masu rai da yawa, abubuwan haɗin matrix na extracellular, da kayan ɗauka. Daga cikin waɗannan, abubuwan da ke haifar da rayuwa kamar su canza yanayin girma-β (TGF-β) da insulin-kamar girma factor-1 (IGF-1) suna taka muhimmiyar rawa wajen daidaita haɓakar ƙwayoyin guringuntsi, rarrabuwa, da haɗakar matrix na extracellular. Abubuwan matrix na waje irin su collagen da hyaluronic acid suna ba da tallafi na jiki don chondrocytes kuma suna shiga cikin daidaita mannewar tantanin halitta, ƙaura, da watsa sigina. Kayayyakin jigilar kayayyaki suna aiki don tattarawa da kuma ci gaba da sakewa da abubuwan da suka shafi bioactive, suna tabbatar da ci gaba da ingancinsu a wurin rauni.
Matsayin Cartalax a Gyaran Rauni na guringuntsi
(1) Inganta Yaduwar Chondrocyte da Bambance-bambance
Abubuwan bioactive a cikin Cartalax, irin su TGF-β da IGF-1, kunna hanyoyin sigina a cikin chondrocytes, inganta ci gaba da sake zagayowar tantanin halitta da kuma ba da damar chondrocytes don canzawa daga lokacin quiescent zuwa lokacin haɓakawa. TGF-β yana ɗaure ga masu karɓar sararin sel don kunna hanyar siginar Smad, tana daidaita maganganun sunadaran da ke da alaƙa da sake zagayowar tantanin halitta, ta haka inganta haɓakar DNA da rarraba tantanin halitta a cikin chondrocytes. IGF-1, a gefe guda, yana hana apoptosis na chondrocyte ta hanyar PI3K-Akt da MAPK hanyoyin sigina yayin inganta haɓakar ƙwayoyin cuta, ƙara yawan chondrocytes, da kuma samar da isasshen salon salula don gyaran guringuntsi.
A lokacin ci gaba na osteoarthritis, phenotype na chondrocytes yana da sauƙi don canzawa, halin da ake kira rage kira na chondrospecific matrix sassa kamar nau'in II collagen da proteoglycans, da kuma ƙara yawan kira na nau'in I collagen da matrix metalloproteinases (MMPs), wanda ke haifar da lalacewar nama na guringuntsi. Cartalax na iya kula da nau'in nau'in chondrocytes na al'ada ta hanyar daidaita siginar siginar ciki da kuma bayanin kwayoyin halitta, yana kula da yanayin al'ada na chondrocytes. TGF-β na iya daidaita ma'anar tsarin SOX9, wanda shine maɓalli mai mahimmanci wanda ke inganta rubutun kwayoyin halitta na nau'in II collagen da proteoglycans, don haka yana riƙe da ikon chondrocytes don haɗawa da ƙayyadaddun abubuwan matrix na guringuntsi. Wasu abubuwan da ke cikin Cartalax kuma na iya hana bayyanar MMPs, rage lalata matrix na waje, da kare mutuncin nama na guringuntsi.
(2) Ka'ida na extracellular matrix metabolism
Matrix na extracellular wani muhimmin sashi ne na nama na guringuntsi, da farko ya ƙunshi collagen, proteoglycans, da fibers na roba. Cartalax yana haɓaka haɗin waɗannan abubuwan matrix na waje ta hanyar kunna hanyoyin siginar anabolic a cikin chondrocytes. Baya ga haɓakar da aka ambata TGF-β-tsakanin haɓakawa na nau'in collagen II da haɓakar proteoglycan, sauran abubuwan haɓakawa a cikin Cartalax, irin su fibroblast girma factor (FGF), kuma na iya yin aiki tare don haɓaka chondrocytes don haɗa ƙarin matrix na waje. FGF yana haɓaka ƙarfin haɓakar furotin a cikin chondrocytes, haɓaka haɓakawa da ɓoye manyan ƙwayoyin cuta irin su collagen da proteoglycans, ta haka ƙara abun ciki na matrix extracellular da haɓaka kaddarorin biomechanical na guringuntsi.
A lokacin gyaran rauni na guringuntsi, gyare-gyaren matrix na extracellular tsari ne mai ƙarfi. Cartalax ba wai kawai yana haɓaka haɓakar matrix na extracellular ba amma kuma yana daidaita tsarin gyaran sa. Yana samun wannan ta hanyar daidaita ma'auni tsakanin matrix metalloproteinases (MMPs) da masu hana su nama (TIMPs). Cartalax yana hana ayyukan MMPs, yana rage yawan lalacewa na matrix extracellular; yana inganta maganganun TIMPs, yana haɓaka tasirin hanawa akan MMPs, don haka yana riƙe da ma'auni na dangi tsakanin haɗuwa da lalata matrix na waje, wanda ya dace da gyaran gyare-gyare da gyaran gyare-gyare na guringuntsi.
(3) Abubuwan da ke hana kumburi
Amsoshi masu kumburi suna taka muhimmiyar rawa a cikin farawa da ci gaba na OA. Cartalax yana da wasu kaddarorin anti-mai kumburi kuma yana iya daidaita maganganun cytokines masu kumburi. A cikin microenvironment mai kumburi, Cartalax na iya hana samar da cytokines pro-mai kumburi irin su tumor necrosis factor-α (TNF-α) da kuma interleukin-1β (IL-1β), yayin da ke haɓaka maganganun cytokines masu kumburi irin su interleukin-10 (IL-10), don haka yana rage kumburin haɗin gwiwa na gida.
Aikace-aikacen Cartalax a cikin Jiyya na Osteoarthritis
(1) Nazarin Gwajin Dabbobi
A cikin gwaje-gwajen dabba, masu bincike sun kafa nau'ikan dabbobin OA daban-daban, irin su samfurin OA na bera wanda aka haifar da transection na gaban cruciate ligament transection (ACLT) da kuma zomo OA samfurin papain ya jawo, don kimanta ingancin Cartalax a gyaran lalacewar guringuntsi. Sakamakon gwaji ya nuna cewa bayan shiga tsakani na Cartalax, bincike na tarihi ya nuna gagarumin ci gaba a cikin santsin guringuntsi na haɗin gwiwa, tsarin salula, da abubuwan matrix na extracellular. Sakamakon lalacewar guringuntsi a cikin rukunin gwaji ya ragu sosai, yana nuna cewa Cartalax yana rage girman lalacewar guringuntsi.
Immunohistochemical da nazarin halittu na kwayoyin halitta sun nuna matakan maganganu na ƙayyadaddun alamomin guringuntsi da kuma rage matakan magana na enzymes masu lalacewa irin su MMPs a cikin ƙungiyar da aka yi wa Cartalax, yana kara tabbatar da rawar da yake takawa wajen inganta gyaran guringuntsi da kuma daidaita tsarin ƙwayar matrix na extracellular.
(2) Binciken Aikace-aikacen Clinical
Ko da yake Cartalax har yanzu yana cikin matakin bincike don aikace-aikacen asibiti, an ba da rahoton wasu karatun asibiti na farko. Ana nuna Cartalax da farko ga marasa lafiya da ƙananan osteoarthritis mai sauƙi zuwa matsakaici, musamman waɗanda aka tabbatar da lalacewar guringuntsi. Don farkon osteoarthritis, inda lalacewar guringuntsi har yanzu yana iya jujjuyawa, Cartalax na iya rage jinkirin ci gaban cuta yadda ya kamata da gyara lalacewar guringuntsi ta hanyar haɓaka haɓakar chondrocyte, daidaita metabolism na matrix extracellular, da rage martani mai kumburi. Hakanan za'a iya amfani da Cartalax azaman haɗin gwiwa don maganin tiyata, ana amfani da shi bayan tiyatar arthroscopic ko aikin gyaran guringuntsi don haɓaka waraka da gyaran nama na guringuntsi.
Kammalawa
A taƙaice, Cartalax yana nuna gagarumin yuwuwar gyara lalacewar guringuntsi a cikin maganin osteoarthritis. Daga tsarin aikin hangen nesa, yana inganta haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar kuma ta daidaita tsarin matrix na matrix, kuma yana haifar da sakamako mai cutarwa, ta haka ne yake gyarawa da kuma kare kullun da ya lalace daga kusurwoyi masu yawa.
Sources
[1] Hu N, Qiu J, Xu B, et al. The Role of Cartilage Stem/Progenitor Cells in Cartilage Repair in Osteoarthritis[J]. Binciken Kwayoyin Tsari na Yanzu, 2023,18(7):892-903.DOI:10.2174/1574888X 17666221006 113739.
[2] McClurg O, Tinson R. Magunguna, 2021,14 (2).DOI:10.3390/ph14020126.
[3] Tanideh N, Borzooeian G, Lotfi M, et al. Sabuwar dabarar gyaran guringuntsi ta hanyar aikace-aikacen P. atlantica tare da sel mai tushe da collagen[J]. Ƙungiyoyin Artificial, 2021,45 (11): 1405-1421.DOI: 10.1111 / aor.14026.
[4] Zuwa K, Romain K, Mak C, et al. The Treatment of Cartilage Damage Using Human Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Systematic Review of in vivo Studies[J]. Iyaka a Bioengineering da Biotechnology, 2020,8:580.DOI:10.3389/fbiee.2020.00580.
[5] Macfarlane E, Seibel MJ, Zhou H. Arthritis da kuma rawar glucocorticoids na endogenous [J]. Binciken Kashi, 2020,8 (1): 33.DOI: 10.1038/s41413-020-00112-2.
[6] Huang LJ, Chen W P. Astaxanthin yana inganta lalacewar guringuntsi a cikin gwajin osteoarthritis [J]. Magungunan Rheumatology na zamani, 2015,25 (5): 768-771.DOI: 10.3109/14397595.2015.1008724.
Samfura akwai don amfani da bincike kawai:
