Los ntawm Cocer Peptides 
1 month ago
Tag nrho cov kab lus thiab cov ntaub ntawv ntawm cov khoom lag luam muab rau ntawm lub vev xaib no tsuas yog rau cov ntaub ntawv tshaj tawm kev tshaj tawm thiab cov hom phiaj kev kawm.
Cov khoom muab rau hauv lub vev xaib no yog npaj tshwj xeeb rau kev tshawb fawb hauv vitro. Kev tshawb fawb hauv vitro (Latin: * hauv iav *, lub ntsiab lus hauv iav) yog ua los ntawm tib neeg lub cev. Cov khoom no tsis yog tshuaj, tsis tau txais kev pom zoo los ntawm US Food and Drug Administration (FDA), thiab yuav tsum tsis txhob siv los tiv thaiv, kho, lossis kho txhua yam mob, kab mob, lossis mob. Nws raug txwv nruj raws li txoj cai los qhia cov khoom no rau hauv tib neeg lossis tsiaj lub cev hauv txhua daim ntawv.
Txheej txheem cej luam
Osteoarthritis (OA) yog ib hom kab mob sib kis uas tshwm sim los ntawm pob txha mos degeneration, subchondral pob txha remodeling, thiab synovial inflammatory teb, ua rau cov neeg mob lub neej zoo. Nrog rau kev nrawm ntawm cov neeg laus, qhov xwm txheej ntawm OA tau nce siab, ua rau muaj kev cuam tshuam rau tib neeg thiab tsev neeg.
Daim duab 1 Kev sib piv ntawm cov yam ntxwv pathological ntawm rheumatoid mob caj dab thiab osteoarthritis. Rheumatoid mob caj dab yog tus cwj pwm los ntawm cov txheej txheem inflammatory mediated los ntawm innate, adaptive, thiab stromal autoimmune teb.
Kev puas tsuaj rau pob txha mos yog ib kauj ruam tseem ceeb hauv kev loj hlob ntawm OA. Vim lub peev xwm kho tus kheej tsawg ntawm pob txha mos, kev puas tsuaj yog qhov nyuaj los kho nws tus kheej, ua rau cov kab mob maj mam zuj zus. Yog li ntawd, kev txheeb xyuas cov txheej txheem zoo rau kev kho pob txha mos tau dhau los ua kev tshawb fawb tsom hauv kev kho OA.
Cartalax yog biopharmaceutical tshwj xeeb tsim los txhawb kev kho pob txha mos. Nws qhov tshwj xeeb muaj pes tsawg leeg sib txawv nyob ntawm qhov kev tshawb fawb sib txawv thiab cov txheej txheem tsim khoom, tab sis nws feem ntau muaj ntau yam bioactive yam, extracellular matrix Cheebtsam, thiab cov khoom nqa khoom. Ntawm cov no, cov khoom siv bioactive xws li hloov pauv qhov kev loj hlob-β (TGF-β) thiab insulin-zoo li kev loj hlob zoo tshaj plaws-1 (IGF-1) ua lub luag haujlwm tseem ceeb hauv kev tswj cov pob txha mos cell proliferation, sib txawv, thiab kev sib txuas ntawm cov cellular matrix. Extracellular matrix Cheebtsam xws li collagen thiab hyaluronic acid muab lub cev kev txhawb nqa rau chondrocytes thiab koom rau hauv kev tswj cov cell adhesion, tsiv teb tsaws, thiab teeb liab transduction. Cov khoom siv thauj khoom ua haujlwm rau encapsulate thiab txhawb nqa-tso tawm bioactive Cheebtsam, ua kom lawv cov kev ua tau zoo tas mus li ntawm qhov chaw raug mob.
Lub luag hauj lwm ntawm Cartalax hauv pob txha pob txha raug mob kho
(1) Txhawb nqa Chondrocyte Proliferation thiab sib txawv
Cov yam ntxwv bioactive hauv Cartalax, xws li TGF-β thiab IGF-1, qhib cov kev taw qhia txoj hauv kev hauv chondrocytes, txhawb nqa lub voj voog ntawm tes thiab ua kom chondrocytes hloov mus rau theem quiescent mus rau theem proliferative. TGF-β khi rau ntawm lub xov tooj ntawm tes receptors los qhib txoj hauv kev Smad signaling, tswj cov kev qhia ntawm lub voj voog ntawm cov proteins, yog li txhawb nqa DNA synthesis thiab cell division hauv chondrocytes. IGF-1, ntawm qhov tod tes, inhibits chondrocyte apoptosis los ntawm PI3K-Akt thiab MAPK kev taw qhia txoj hauv kev thaum txhawb kev loj hlob ntawm tes, nce cov chondrocytes, thiab muab cov cellular txaus rau kho pob txha mos.
Thaum lub sij hawm kev loj hlob ntawm osteoarthritis, lub phenotype ntawm chondrocytes yog nquag hloov, tus cwj pwm los ntawm txo synthesis ntawm chondrospecific matrix Cheebtsam xws li hom II collagen thiab proteoglycans, thiab nce synthesis ntawm hom I collagen thiab matrix metalloproteinases (MMPs), ua rau pob txha mos degeneration. Cartalax tuaj yeem tswj hwm cov phenotype ntawm chondrocytes los ntawm kev tswj cov teeb liab intracellular transduction thiab noob qhia, tswj cov phenotype ntawm chondrocytes. TGF-β tuaj yeem txhim kho qhov kev qhia ntawm SOX9 noob, uas yog qhov tseem ceeb ntawm kev hloov pauv uas txhawb nqa cov noob hloov ntawm hom II collagen thiab proteoglycans, yog li tswj lub peev xwm ntawm chondrocytes los ua ke cov pob txha mos tshwj xeeb matrix. Qee cov khoom siv hauv Cartalax tuaj yeem cuam tshuam qhov kev qhia ntawm MMPs, txo qhov degradation ntawm extracellular matrix, thiab tiv thaiv kev ncaj ncees ntawm cov pob txha mos.
(2) Kev tswj hwm ntawm extracellular matrix metabolism
Lub extracellular matrix yog ib qho tseem ceeb ntawm cov pob txha mos, feem ntau muaj collagen, proteoglycans, thiab elastic fibers. Cartalax txhawb cov synthesis ntawm cov extracellular matrix Cheebtsam los ntawm activating anabolic signaling txoj kev nyob rau hauv chondrocytes. Ntxiv rau qhov tau hais los saum no TGF-β-mediated txhawb nqa ntawm hom II collagen thiab proteoglycan synthesis, lwm yam kev loj hlob hauv Cartalax, xws li fibroblast kev loj hlob (FGF), kuj tuaj yeem ua synergistically txhawb chondrocytes los ua ke ntau extracellular matrix. FGF txhim kho cov protein synthesis muaj peev xwm nyob rau hauv chondrocytes, txhawb cov synthesis thiab secretion ntawm loj molecules xws li collagen thiab proteoglycans, yog li ua rau cov ntsiab lus ntawm extracellular matrix thiab txhim kho cov biomechanical zog ntawm pob txha mos.
Thaum lub sij hawm kho pob txha mos, kev kho mob ntawm extracellular matrix yog ib tug dynamic txheej txheem. Cartalax tsis tsuas yog txhawb nqa cov synthesis ntawm extracellular matrix tab sis kuj tswj nws cov txheej txheem kho dua tshiab. Nws ua tiav qhov no los ntawm kev tswj hwm qhov sib npaug ntawm matrix metalloproteinases (MMPs) thiab lawv cov ntaub so ntswg inhibitors (TIMPs). Cartalax inhibits qhov kev ua ntawm MMPs, txo qis kev degradation ntawm extracellular matrix; Nws txhawb nqa kev qhia ntawm TIMPs, txhim kho lawv cov nyhuv inhibitory ntawm MMPs, yog li tswj hwm qhov sib npaug ntawm kev sib txuas thiab degradation ntawm extracellular matrix, uas yog qhov zoo rau kev kho kom zoo thiab kho cov pob txha mos.
(3) Anti-inflammatory teebmeem
Cov lus teb mob ua rau lub luag haujlwm tseem ceeb hauv qhov pib thiab kev loj hlob ntawm OA. Cartalax muaj qee yam tshuaj tiv thaiv kab mob thiab tuaj yeem tswj cov kev qhia ntawm inflammatory cytokines. Nyob rau hauv lub inflammatory microenvironment, Cartalax tuaj yeem inhibit qhov tsim ntawm pro-inflammatory cytokines xws li qog necrosis factor-α (TNF-α) thiab interleukin-1β (IL-1β), thaum upregulating cov lus qhia ntawm anti-inflammatory cytokines xws li interleukin-10 (IL-10), yog li alleviating lub zos microenviage mob.
Kev siv Cartalax hauv Kev Kho Mob Osteoarthritis
(1) Kev Tshawb Fawb Tsiaj
Hauv kev sim tsiaj, cov kws tshawb fawb tau tsim ntau yam OA tsiaj qauv, xws li tus nas OA qauv ntxias los ntawm anterior cruciate ligament transection (ACLT) thiab luav OA qauv induced los ntawm papain, los ntsuas qhov ua tau zoo ntawm Cartalax hauv kev kho pob txha mos. Cov txiaj ntsig tau sim pom tias tom qab Cartalax cuam tshuam, kev soj ntsuam histological tau qhia txog kev txhim kho tseem ceeb hauv kev sib koom ua ke pob txha mos saum npoo smoothness, cellular qauv, thiab extracellular matrix Cheebtsam. Cov qhabnias ntawm pob txha mos hauv pawg sim tau txo qis, qhia tias Cartalax txo qhov hnyav ntawm pob txha mos.
Immunohistochemical thiab molecular lom tshuaj ntsuam xyuas tau nthuav tawm qhia qib siab ntawm cov pob txha mos tshwj xeeb cov cim thiab txo qis kev qhia qib ntawm degradative enzymes xws li MMPs hauv Cartalax-kho pab pawg, ntxiv kev lees paub nws lub luag haujlwm hauv kev txhawb nqa pob txha mos thiab tswj cov txheej txheem metabolic hauv cov cellular.
(2) Clinical Application Exploration
Txawm hais tias Cartalax tseem nyob rau theem tshawb nrhiav rau kev siv tshuaj kho mob, qee qhov kev tshawb fawb soj ntsuam ua ntej tau tshaj tawm. Cartalax feem ntau qhia rau cov neeg mob uas mob me mus rau nruab nrab osteoarthritis, tshwj xeeb tshaj yog cov neeg uas paub tseeb tias pob txha mos puas. Rau osteoarthritis thaum ntxov, qhov uas pob txha pob txha puas tseem thim rov qab, Cartalax tuaj yeem ua rau cov kab mob qeeb qeeb thiab kho pob txha mos los ntawm kev txhawb nqa chondrocyte proliferation, regulating extracellular matrix metabolism, thiab txo cov inflammatory teb. Cartalax kuj tseem siv tau los ua kev kho mob phais, siv tom qab phais arthroscopic lossis kho pob txha mos los txhawb kev kho thiab kho cov pob txha mos.
Xaus
Hauv cov ntsiab lus, Cartalax qhia txog lub peev xwm tseem ceeb hauv kev kho pob txha mos hauv kev kho mob osteoarthritis. Los ntawm cov txheej txheem ntawm kev saib xyuas, nws txhawb nqa pob txha mos ntawm cov pob txha pob txha loj hlob thiab kev sib txawv, tswj cov txheej txheem ntawm cov txheej txheem matrix metabolism, thiab ua kom muaj kev tiv thaiv kab mob, yog li kho thiab tiv thaiv pob txha mos los ntawm ntau lub kaum ntse ntse.
Qhov chaw
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[2] McClurg O, Tinson R, Troeberg L. Targeting pob txha mos degradation hauv Osteoarthritis[J]. Pharmaceuticals, 2021,14(2).DOI:10.3390/ph14020126.
[3] Tanideh N, Borzooeian G, Lotfi M, et al. Lub tswv yim tshiab ntawm kev kho pob txha mos ntawm daim ntawv thov ntawm P. atlantica nrog cov qia hlwb thiab collagen[J]. Artificial Organs, 2021,45(11):1405-1421.DOI:10.1111/aor.14026.
[4] To K, Romain K, Mak C, et al. Kev kho mob pob txha mos siv tib neeg Mesenchymal Stem Cell-Drived Extracellular Vesicles: Kev Tshawb Fawb Txog Kev Tshawb Fawb hauv vivo [J]. Frontiers hauv Bioengineering thiab Biotechnology, 2020, 8: 580.DOI: 10.3389/fbioe.2020.00580.
[5] Macfarlane E, Seibel MJ, Zhou H. Kev mob caj dab thiab lub luag haujlwm ntawm endogenous glucocorticoids [J]. Kev tshawb fawb pob txha, 2020,8(1):33.DOI:10.1038/s41413-020-00112-2.
[6] Huang LJ, Chen W P. Astaxanthin ameliorates pob txha mos puas hauv kev sim osteoarthritis[J]. Niaj hnub nimno Rheumatology, 2015,25(5):768-771.DOI:10.3109/14397595.2015.1008724.
Cov khoom muaj rau kev tshawb fawb siv nkaus xwb:
