TB4 fragment 17-13 10 mg

▎ TB4 fragment(17-13) Structure

Source: PubChem

Sequence: LKKTETQ Molecular Formula: C 36H 66N 10O13 Molecular Luj: 847.0 g / mol CAS Nr .: 476014-70-7 PIB: 101697888 Synonyms: Fequesetide; HY-P2463; LKKTETQ

▎ TB4 fragment(17-13) Kev tshawb fawb

Cov txheej txheem ntawm kev ua ntawm TB4 fragment (17-13) yog dab tsi?

1. Mechanisms ntawm kev txhawb cov ntaub so ntswg regeneration

TB4 fragment (17-13) plays lub luag haujlwm tseem ceeb hauv kev txhawb nqa cov ntaub so ntswg. Nws tuaj yeem ua tiav qhov haujlwm no los ntawm cov txheej txheem hauv qab no:

Stimulating cell proliferation thiab sib txawv

Cov kab no tuaj yeem khi rau cov receptors tshwj xeeb ntawm lub xovtooj ntawm tes thiab ua kom cov kev taw qhia intracellular, yog li txhawb nqa cell proliferation thiab sib txawv. Piv txwv li, nws tuaj yeem ua rau txoj kev taw qhia txog kev loj hlob ntawm tes thiab kev faib tawm, xws li mitogen-activated protein kinase (MAPK) txoj kev thiab phosphatidylinositol 3-kinase (PI3K) / protein kinase B (Akt) txoj kev ^[1] . Kev ua kom cov kab ke no tuaj yeem txhawb txoj kev loj hlob ntawm lub voj voog ntawm tes, ua kom cov xov tooj ntawm tes, thiab yog li txhawb nqa cov ntaub so ntswg.

Tsis tas li ntawd, TB4 fragment (17-13) kuj tseem tuaj yeem txhawb kev sib txawv ntawm tes los ntawm kev tswj cov kev qhia ntawm cov noob tshwj xeeb. Nws tuaj yeem cuam tshuam rau kev ua haujlwm ntawm cov txheej txheem hloov pauv cuam tshuam nrog kev sib txawv ntawm tes, yog li tswj kev sib txawv ntawm cov hlwb hauv ib qho kev taw qhia tshwj xeeb thiab muab ntau hom hlwb uas xav tau rau cov ntaub so ntswg rov ua dua tshiab.

Txhawb nqa angiogenesis

Kev tsim cov ntaub so ntswg feem ntau xav tau cov ntshav txaus, thiab angiogenesis yog txheej txheem tseem ceeb rau kev muab ntshav. TB4 fragment (17-13) tuaj yeem txhawb kev tsim cov angiogenesis los ntawm kev txhawb nqa kev loj hlob, kev tsiv teb tsaws, thiab lumen tsim ntawm vascular endothelial hlwb. Nws tuaj yeem qhib qhov kev qhia ntawm pro-angiogenic yam xws li vascular endothelial growth factor (VEGF), yog li txhawb kev tsim cov hlab ntsha tshiab ^[1] . Cov hlab ntsha uas tau tsim tshiab tuaj yeem muab cov pa oxygen, cov khoom noj khoom haus, thiab kev loj hlob tuaj rau cov ntaub so ntswg puas, ua kom cov ntaub so ntswg rov ua dua tshiab.

2. Mechanisms ntawm exerting anti-inflammatory teebmeem

Kev mob yog ib qho kev tiv thaiv ntawm lub cev rau kev raug mob lossis kev kis kab mob, tab sis cov lus teb ntau dhau tuaj yeem ua rau cov ntaub so ntswg puas thiab ua haujlwm tsis zoo. TB4 fragment (17-13) muaj kev tiv thaiv kab mob, thiab nws cov txheej txheem yuav suav nrog cov hauv qab no:

Inhibiting lub activation thiab recruitment ntawm inflammatory cells

Inflammatory cov lus teb feem ntau koom nrog kev ua kom muaj zog thiab nrhiav neeg ua haujlwm ntawm ntau lub hlwb, xws li neutrophils, macrophages, thiab lymphocytes. TB4 fragment (17-13) yuav txo tau cov inflammatory teb los ntawm inhibiting lub activation thiab recruitment ntawm cov inflammatory hlwb. Piv txwv li, nws yuav inhibit qhov kev qhia ntawm receptors nyob rau saum npoo ntawm inflammatory hlwb, txo cov lus teb ntawm inflammatory hlwb rau inflammatory signals; los yog los ntawm kev tswj cov kev tsiv teb tsaws chaw ntawm cov kab mob inflammatory, txo cov neeg ua haujlwm ntawm cov kab mob inflammatory mus rau qhov chaw puas ^[1].

Tsis tas li ntawd, qhov tawg no kuj tseem tuaj yeem cuam tshuam cov txheej txheem metabolic ntawm cov hlwb inflammatory thiab inhibit cov haujlwm ntawm cov hlwb. Piv txwv li, nws tuaj yeem tswj hwm lub zog metabolism ntawm cov hlwb inflammatory, txo qhov tsim cov pa oxygen reactive (ROS) thiab pro-inflammatory cytokines los ntawm inflammatory cells, thiab yog li txo cov inflammatory teb.

Kev tswj cov kev qhia thiab kev tso tawm ntawm cov neeg kho mob inflammatory

Thaum lub sij hawm inflammatory teb, ntau yam inflammatory mediators raug tsim, xws li cytokines, chemokines, thiab prostaglandins. Cov kws kho mob inflammatory no tuaj yeem ua kom cov lus teb inflammatory ntxiv, ua rau cov ntaub so ntswg puas. TB4 fragment (17-13) tuaj yeem ua rau muaj kev tiv thaiv kab mob los ntawm kev tswj cov lus qhia thiab tso tawm cov neeg kho mob inflammatory. Piv txwv li, nws yuav inhibit qhov kev qhia ntawm pro-inflammatory cytokines, xws li qog necrosis factor-α (TNF-α), interleukin-1β (IL-1β), thiab interleukin-6 (IL-6); thiab tib lub sijhawm, txhawb kev qhia ntawm cov tshuaj tiv thaiv cytokines, xws li interleukin-10 (IL-10) ^[1].

Tsis tas li ntawd, qhov tawg no kuj tuaj yeem cuam tshuam rau kev qhia ntawm chemokines, txo cov neeg ua haujlwm ntawm cov hlwb inflammatory; los yog tswj cov synthesis ntawm prostaglandins, txo cov inflammatory teb.

3. Mechanisms ntawm kev ua tiav kev kho sai

Cov txheej txheem ntawm kev txiav txim ntawm TB4 fragment (17-13) hauv kev kho sai yuav cuam tshuam nrog nws cov teebmeem ntawm kev txhawb nqa cov ntaub so ntswg rov ua dua tshiab thiab tiv thaiv kab mob. Tshwj xeeb, nws tuaj yeem ua tiav kho sai los ntawm cov hauv qab no:

Accelerating synthesis thiab remodeling ntawm extracellular matrix

Extracellular matrix yog ib feem tseem ceeb ntawm cov ntaub so ntswg thiab tseem ceeb heev rau kev tswj cov qauv thiab kev ua haujlwm ntawm cov ntaub so ntswg. TB4 fragment (17-13) tuaj yeem ua kom lub cev kho cov ntaub so ntswg puas los ntawm kev txhawb nqa cov khoom sib txuas ntawm cov khoom siv sab nraud, xws li collagen, elastin, thiab glycosaminoglycans. Nws tuaj yeem qhib cov kev taw qhia txog kev sib txuas ntawm cov khoom siv hluav taws xob ntxiv, txhawb cov hlwb los ua ke thiab tso tawm cov khoom sib txuas ntxiv ntawm cov matrix ^[1].

Tsis tas li ntawd, qhov tawg no kuj tseem tuaj yeem tswj cov txheej txheem hloov kho ntawm extracellular matrix. Thaum lub sij hawm kho cov ntaub so ntswg, lub extracellular matrix yuav tsum tau hloov kho tsis tu ncua kom haum rau kev loj hlob thiab kho cov ntaub so ntswg. Lub TB4 fragment (17-13) tuaj yeem tswj qhov sib npaug ntawm qhov degradation thiab synthesis ntawm extracellular matrix los ntawm kev tswj cov kev ua ntawm enzymes xws li matrix metalloproteinases (MMPs), txhawb kev kho sai ntawm cov ntaub so ntswg.

Txhim kho cell migration thiab adhesion muaj peev xwm

Kev tsiv teb tsaws thiab adhesion muaj peev xwm ntawm cov hlwb kuj tseem ceeb heev rau kev kho cov ntaub so ntswg. TB4 fragment (17-13) tuaj yeem txhim kho kev tsiv teb tsaws thiab adhesion muaj peev xwm ntawm cov hlwb los ntawm kev tswj cov kev qhia thiab kev ua haujlwm ntawm cov cell nto receptors. Piv txwv li, nws yuav txhawb kev nthuav qhia ntawm cell adhesion molecules xws li integrins, ua kom cov adhesion ntawm cov hlwb thiab cov extracellular matrix; los yog tswj lub reorganization ntawm cytoskeleton, txhawb cell migration ^[1].

Kev tsiv teb tsaws ntawm cov hlwb tuaj yeem ua rau cov hlwb nyob ib puag ncig cov ntaub so ntswg puas sai sai mus txog qhov chaw puas thiab koom nrog cov txheej txheem kho cov ntaub so ntswg; thiab cov adhesion ntawm cov hlwb tuaj yeem ua kom ntseeg tau tias cov hlwb ruaj khov ua lub luag haujlwm ntawm qhov chaw puas, txhawb kev kho sai ntawm cov ntaub so ntswg.

HCD MS / MS kaw ntawm 20eV (a) thiab 700MHz-NMR (b) spectra ntawm TB4.

Source: PubMed ^[1]

Yuav ua li cas TB4 fragment (17-13) tswj lub paj hlwb kom txhim kho kev ua kis las thiab txo kev qaug zog?

Kev txiav txim ncaj qha rau cov neurons

Lub TB4 fragment (17-13) tuaj yeem ua ncaj qha rau cov neurons, cuam tshuam rau kev ua kom muaj zog, kev sib kis ntawm synaptic, thiab plasticity ntawm neurons. Piv txwv li, nws yuav khi rau receptors ntawm qhov chaw ntawm neurons, qhib intracellular signaling pathways, thiab tswj kev ua haujlwm ntawm neurons.

Qee qhov kev tshawb fawb tau pom tias thymosin β4 tuaj yeem khi rau ntau yam receptors, suav nrog integrin receptors thiab kev loj hlob receptors, uas ua lub luag haujlwm tseem ceeb hauv kev txhim kho thiab kev ua haujlwm ntawm lub paj hlwb ^[1].

Indirect kev tswj ntawm lub paj hlwb los ntawm kev tiv thaiv kab mob

Muaj kev sib raug zoo ntawm lub cev tiv thaiv kab mob thiab lub paj hlwb. Cov kab mob TB4 (17-13) tuaj yeem cuam tshuam ncaj qha rau lub paj hlwb los ntawm kev tswj hwm kev ua haujlwm ntawm lub cev tsis muaj zog. Piv txwv li, nws tuaj yeem cuam tshuam rau kev ua haujlwm thiab kev tso tawm ntawm lub cev tiv thaiv kab mob, tswj cov qib ntawm cov kab mob inflammatory, thiab yog li cuam tshuam rau kev ua haujlwm ntawm lub paj hlwb.

Qee cov kev tshawb fawb tau pom tias cov kab mob inflammatory ua haujlwm tseem ceeb hauv cov kab mob paj hlwb thiab qaug zog. TB4 fragment (17-13) yuav txo tau cov inflammatory teb ntawm lub paj hlwb thiab txhim kho kev ua haujlwm ntawm lub paj hlwb los ntawm kev tswj cov theem ntawm inflammatory yam ^[1].

Kev cuam tshuam ntawm neuroendocrine system

Cov kab mob neuroendocrine ua lub luag haujlwm tseem ceeb hauv kev tswj hwm lub cev lub cev kev ua haujlwm thiab kev ntxhov siab. TB4 fragment (17-13) tuaj yeem tswj cov haujlwm ntawm lub paj hlwb los ntawm kev cuam tshuam rau kev ua haujlwm ntawm cov kab mob neuroendocrine. Piv txwv li, nws tuaj yeem cuam tshuam rau kev ua haujlwm ntawm hypothalamic-pituitary-adrenal axis, tswj kev tso tawm ntawm cov tshuaj hormones kev ntxhov siab xws li cortisol, thiab yog li cuam tshuam rau kev ua haujlwm ntawm lub paj hlwb.

Qee qhov kev tshawb fawb tau pom tias qib ntawm cov tshuaj hormones kev ntxhov siab cuam tshuam nrog kev xav ntawm qaug zog thiab kev ua kis las. TB4 fragment (17-13) tuaj yeem txo qhov kev qaug zog thiab txhim kho kev ua kis las los ntawm kev tswj hwm qib ntawm cov tshuaj hormones kev ntxhov siab ^[1].

Dab tsi yog qhov cuam tshuam ntawm TB4 fragment (17-13)?

1. Cov ntaub so ntswg kho thiab rov tsim dua tshiab

Kev kho mob ntawm cov mob hnyav thiab mob ntev: 

Rau cov qhov txhab mob hnyav (xws li phais phais, kub hnyiab) thiab cov qhov txhab mob ntev (xws li mob ntshav qab zib rwj), TB4 fragment (17-13) tuaj yeem txo qhov caws pliav. Nws cov txheej txheem ntawm kev ua haujlwm yuav cuam tshuam rau kev txhawb nqa cov txheej txheem ntawm collagen thiab tswj cov kab mob inflammatory ^[2].

Cov ntaub so ntswg regeneration qhia nyob rau hauv cov tsiaj sim: 

Hauv kev sim tsiaj, cov peptide fragment no tuaj yeem txhawb kev tsim kho ntawm daim tawv nqaij, leeg, leeg, thiab pob txha mos. Piv txwv li, hauv cov qauv kev raug mob ntawm daim tawv nqaij, TB4 fragment (17-13) tuaj yeem txhawb kev tsiv teb tsaws thiab kev loj hlob ntawm keratinocytes, ua kom lub qhov txhab zoo (Ho ENM, 2012). Rau cov leeg thiab cov leeg raug mob, nws tuaj yeem txhawb kev kho cov ntaub so ntswg los ntawm kev txhawb nqa kev ua kom thiab loj hlob ntawm cov hlwb satellite ^[3] . Hauv cov qauv kev raug mob ntawm pob txha mos, qhov peptide fragment no tuaj yeem txhawb cov pob txha mos rov qab los ntawm kev tswj cov metabolism thiab kev ua haujlwm ntawm chondrocytes ^[2].

2. Anti-inflammatory zog

Hauv cov kab mob inflammatory (xws li mob caj dab rheumatoid, Crohn tus kab mob), cov lus teb inflammatory tshwm sim, ua rau cov ntaub so ntswg puas thiab lub cev tsis ua haujlwm. Raws li kev kho mob ntxiv, TB4 fragment (17-13) tuaj yeem txo cov tsos mob ntawm tus kab mob thiab ncua kev kis kab mob los ntawm inhibiting qhov inflammatory teb ^[4].

3. Rehabilitation ntawm musculoskeletal raug mob

TB4 fragment (17-13) accelerates kho cov nqaij ntshiv, tendonitis, thiab ligament raug mob, shortening lub sij hawm rehabilitation ntawm ncaws pob. Hauv cov tshuaj kis las, kev raug mob musculoskeletal yog cov teeb meem tshwm sim. Cov peptide fragment no tuaj yeem txhawb kev kho cov ntaub so ntswg puas ^[3].

4. Kho mob plawv

Tom qab myocardial infarction, TB4 fragment (17-13) tuaj yeem txhawb nqa angiogenesis thiab kev ciaj sia ntawm cardiomyocytes, txhim kho kev ua haujlwm plawv. Myocardial infarction yog ib yam kab mob uas tshwm sim los ntawm kev txhaws ntawm cov hlab ntsha, ua rau myocardial ischemia thiab hypoxia. Angiogenesis tuaj yeem muab cov pa oxygen thiab cov as-ham rau cov myocardium puas, txhawb kev ciaj sia thiab kho cov cardiomyocytes ^[5].

Hauv cov qauv tsiaj, TB4 fragment (17-13) txo qhov loj ntawm infarct thiab txhim kho myocardial. Nws cov txheej txheem ntawm kev ua haujlwm yuav cuam tshuam txog kev txhawb nqa kev qhia thiab tso tawm ntawm kev loj hlob yam xws li vascular endothelial growth factor (VEGF).

5. Neuroprotection thiab hlwb noj qab haus huv

TB4 fragment (17-13) inhibits neuronal apoptosis thiab txhawb kev loj hlob ntawm neural qia hlwb. Neuronal apoptosis yog ib qho tseem ceeb ntawm cov txheej txheem pathological ntawm cov kab mob neurodegenerative thiab mob hlwb. Cov peptide fragment no tuaj yeem cuam tshuam cov neuronal apoptosis los ntawm kev tswj cov kab mob hauv lub cev. Nyob rau tib lub sijhawm, txhawb kev loj hlob ntawm neural qia hlwb tuaj yeem muab lub hauv paus tshiab ntawm cov hlwb rau cov paj hlwb puas, txhawb kev kho paj hlwb.

Hauv kev mob stroke, mob hlwb raug mob (TBI), thiab cov kab mob neurodegenerative (xws li Alzheimer's kab mob), cov paj hlwb raug puas tsuaj rau ntau qib. TB4 fragment (17-13) tuaj yeem pab txhawb kev rov zoo ntawm cov neeg mob los ntawm nws cov neuroprotective thiab cov paj hlwb kho-txhim kho cov teebmeem ^[4].

6. Kev kho mob ntawm qhov muag

0.1% Tβ4 qhov muag tee tag nrho kho qhov txhab thiab txo cov tsos mob ntawm 6 tus neeg mob neurotrophic keratitis. Neurotrophic keratitis yog ib hom kab mob pob txha uas tshwm sim los ntawm kev puas hlwb pob txha. TB4 fragment (17-13) tuaj yeem txhawb txoj kev kho mob ntawm pob txha pob txha los ntawm kev txhawb nqa kev loj hlob thiab kev tsiv teb tsaws ntawm pob txha epithelial hlwb thiab tswj cov lus teb inflammatory ^[6].

7. Cov plaub hau rov tsim dua tshiab

TB4 fragment (17-13) activates cov plaub hau follicle qia hlwb thiab prolongs lub anagen theem ntawm cov plaub hau kev loj hlob. Cov plaub hau follicle qia hlwb yog cov hlwb tseem ceeb rau kev tsim cov plaub hau. Lawv tuaj yeem sib txawv rau ntau hom ntawm tes ntawm cov hauv paus plaub hau, txhawb kev loj hlob thiab kev loj hlob ntawm cov plaub hau. Cov peptide fragment no tuaj yeem txhawb cov plaub hau rov tsim dua tshiab los ntawm kev tswj cov kev loj hlob thiab kev sib txawv ntawm cov plaub hau follicle qia hlwb (Pan DY, 2021).

Tsiaj sim

Cov nas: 

Cov plaub hau loj hlob sai sai. Hauv cov qauv nas, TB4 fragment (17-13) tuaj yeem txhawb kev loj hlob thiab kev loj hlob ntawm cov plaub hau los ntawm kev ua kom cov plaub hau follicle qia hlwb ^[4].

tshis: 

Ua kom tus naj npawb ntawm cov hauv paus plaub hau thib ob thiab txhawb kev tsim cov plaub hau. Nyob rau hauv tus qauv tshis, qhov peptide fragment yuav txhawb txoj kev loj hlob ntawm cov plaub hau los ntawm kev tswj lub voj voog loj hlob ntawm cov hauv paus plaub hau thiab kev loj hlob ntawm cov plaub hau ^[4].

Hauv kev xaus, TB4 fragment (17-13) tuaj yeem txhawb kev kho cov ntaub so ntswg, tiv thaiv kab mob, thiab kev tsim ntawm tes, ua kom lub qhov txhab zoo, thiab txhim kho musculoskeletal muaj nuj nqi. Nws yog ib qho tseem ceeb heev rau cov tshuaj regenerative, coj kev cia siab tshiab rau kev kho mob ntawm cov kab mob xws li kub hnyiab thiab raug mob ntawm cov leeg thiab kev tshawb fawb thiab kev loj hlob ntawm cov tshuaj tiv thaiv kab mob, nrog rau daim ntawv thov dav dav.

Hais Txog Tus Sau

Cov ntaub ntawv hais saum toj no yog txhua yam tshawb fawb, kho thiab sau los ntawm Cocer Peptides.

Scientific Journal Author

Choi B yog tus kws tshawb fawb tshwj xeeb thiab kev kawm nrog kev koom tes tseem ceeb rau cov tsev kawm muaj koob npe zoo li Yonsei University thiab Seoul National University (SNU). Nws txoj haujlwm thoob plaws lub tsev kawm ntawv no tau ua qhov tseem ceeb hauv kev nce qib ntau yam kev kawm thiab kev tshawb fawb.

Nws txoj kev tshawb fawb nyiam thiab kev txawj ntse hla thoob plaws ntau hom kev kawm. Cov no suav nrog Microbiology, Biotechnology & Applied Microbiology, Immunology, Pharmacology & Pharmacy, thiab Science & Technology - Lwm Yam. Nws txoj kev tshawb fawb dav dav hauv cov cheeb tsam no qhia txog nws txoj kev paub dav dav thiab kev koom tes rau hauv zej zog kev tshawb fawb. Choi B tau teev nyob rau hauv qhov kev siv ntawm cov ntaub ntawv pov thawj [2].

▎ Cov Lus Qhia Tseem Ceeb

[1] Esposito S, Deventer K, Goeman J, et al. Synthesis thiab characterization ntawm N-terminal acetylated 17-23 fragment ntawm thymosin beta 4 qhia nyob rau hauv TB4, ib yam khoom uas xav tias yuav muaj peev xwm doping [J]. Kev Ntsuas Tshuaj thiab Tshuaj Ntsuam Xyuas, 2012,4(9): 733-738.DOI: 10.1002/dta.1402.

[2] Choi B. Archives of Pharmacy Research, 2023,46(2):78-89.DOI:10.1007/s12272-023-01428-3.

[3] Llgen HLO, Llgen RML O. Health and Medicine in the future: change through sports medicine. Kev Kho Mob Kev Ua Si hauv kev hloov pauv, 2018[C]. https://www.semanticscholar.org/paper/Health-and-Medicineinthefuture%3AchangethroughL%C3%B6llgenL%C3%B6llgen/a5d6430c8b5442f02bc3238fa48ec0e657489942

[4] Pan DY, Xu LY, Li E M. Post-translational modification of TB4 and its functional regulation[J]. Chemistry of Life, 2021,41(04:734-740.DOI:10.13488/j.smhx.20200532.

[5] Eming S A. Evolution of immune pathways in regeneration and repair: Recent concepts and translational perspectives [J]. Seminars in Immunology, 2014,26(4):275-276.DOI:10.1016/j.smim.2014.09.001.

[6] Ho ENM, Kwok WH, Lau MY, et al. Doping tswj kev tsom xam ntawm TB4, ib qho hluavtaws version ntawm thaj tsam nquag ntawm thymosin β4, hauv cov zis equine thiab ntshav los ntawm cov kua chromatography-mass spectrometry [J]. Phau ntawv Journal of Chromatography A, 2012,1265:57-69.DOI:10.1016/j.chroma.2012.09.043.

Tag nrho cov kab lus thiab cov ntaub ntawv ntawm cov khoom lag luam muab rau ntawm lub vev xaib no tsuas yog rau cov ntaub ntawv tshaj tawm kev tshaj tawm thiab cov hom phiaj kev kawm.  

Cov khoom muab rau hauv lub vev xaib no yog npaj tshwj xeeb rau kev tshawb fawb hauv vitro. Kev tshawb fawb hauv vitro (Latin: * hauv iav *, lub ntsiab lus hauv iav) yog ua los ntawm tib neeg lub cev. Cov khoom no tsis yog tshuaj, tsis tau txais kev pom zoo los ntawm US Food and Drug Administration (FDA), thiab yuav tsum tsis txhob siv los tiv thaiv, kho, lossis kho txhua yam mob, kab mob, lossis mob. Nws raug txwv nruj raws li txoj cai los qhia cov khoom no rau hauv tib neeg lossis tsiaj lub cev hauv txhua daim ntawv.