Cocer Peptides ye 
ŋlɔe ɣleti 1 enye sia
NYATAKAKAWO KPLE NUSIWO WOTSƆ ƑE NYATAKAKA SIWO KAtã WOÐO LE NYATAKAKADZRAÐOƑE SIA ME LA KPLE NYATAKAKAWO kaka KPLEE NUFIAFIA ƑE TAÐOÐOWO KO.
Wotrɔ asi le atike siwo wona le nyatakakadzraɖoƒe sia ŋu na numekuku le vitro ɖeɖeko. Wowɔa numekuku le vitro (Latingbe me: *le ahuhɔ̃e me*, si gɔmee nye le ahuhɔ̃e me) le amegbetɔ ƒe ŋutilã godo. Atike siawo menye atikewo o, United States ƒe Nuɖuɖu Kple Atike Ŋuti Dɔwɔƒe (FDA) meda asi ɖe wo dzi o, eye mele be woazã wo atsɔ axe mɔ ɖe atikewɔwɔ, dɔléle, alo dɔléle aɖeke nu, adae, alo adae o. Se de se vevie be woatsɔ atike siawo ade amegbetɔ alo lãwo ƒe ŋutilã me le mɔ ɖesiaɖe nu.
Kpɔkplɔyiɖeme
TB 500 (Thymosin Beta-4 Fragment 17–23) nye bioactive fragment si do ƒome kplikplikpli kple dɔwɔwɔ vevi geɖewo le amegbetɔ ƒe ŋutilãŋutinunya me, vevietɔ le lãmenugbagbeviwo ɖɔɖɔɖo kple lãmenugbagbeviwo ƒe ʋuʋu me.
Nɔnɔmetata 1 TB 500 Fragment (17–23) ƒe atikewo ƒe wɔwɔme.
Nyatakaka Veviwo tso TB 500 ƒe Kakɛ ŋu (17–23) .
TB 500 Kakɛ (17–23) nye amegbetɔ ƒe thymosin beta-4 (Thymosin Beta-4) ƒe N-nuwuƒe ƒe acetylated 17–23 kakɛ. N-terminal acetylation wɔa akpa vevi aɖe le protein-protein ƒe kadodo kple lãmenugbagbeviwo me nɔnɔ dzi kpɔkpɔ me. Acetyl ƒuƒoƒowo ƒe anyinɔnɔ ate ŋu atrɔ molecule ƒe dzoxɔxɔ ƒe mama kple teƒe ƒe nɔnɔme, si ana wòakpɔ ŋusẽ ɖe eƒe ŋutete be wòabla kple biomolecules bubuwo dzi. Le amegbetɔ ƒe ŋutilã me la, thymosin β4 nye peptide si kaka ɖe teƒe geɖe, eye woateŋu awɔ TB 500 Fragment (17–23), abe eƒe kakɛ tɔxɛ ene, to endogenous proteolytic processes me.
TB 500 ƒe Kakɛ ƒe Dɔwɔwɔ ƒe Mɔnu (17–23) .
(1) Lãmenugbagbeviwo ƒe Ʋuʋu Kple Chemotaxis
Ŋusẽkpɔɖeamedzi ɖe Lãmenugbagbevi siwo le lãmenugbagbeviwo me dzi: TB 500 Fragment (17–23) doa lãmenugbagbeviwo ƒe ʋuʋu ɖe ŋgɔ, si nye ɖoɖo vevi aɖe na ʋukawo ƒe dzɔdzɔ. Le abi ƒe dɔyɔyɔ alo lãmenugbagbeviwo ɖɔɖɔɖo me la, ele be lãmenugbagbevi siwo le lãkusi me naʋu ayi teƒe siwo gblẽ be woawɔ ʋuka yeyewo, be woatsɔ ɔksidzin kple nunyiame ana lãkusia. TB 500 Fragment (17–23) ate ŋu ana lãmenugbagbeviwo ƒe ʋuʋu nanyo ɖe edzi to lãmenugbagbeviwo me dzesimɔ siwo kplɔ wo nɔewo ɖo ƒe dɔwɔwɔ me, abe PI3K-Akt dzesimɔ ene, si kpɔa lãmenugbagbeviwo ƒe lãmenugbagbeviwo ƒe ƒunukpeƒe ƒe gbugbɔgaɖoɖo dzi. PI3K-Akt ƒe dzesimɔa ƒe dɔwɔwɔ doa actin ƒe polymerization kple depolymerization ɖe ŋgɔ, si wɔnɛ be lãmenugbagbeviwo te ŋu kekea pseudopodia ɖe enu eye woyia ŋgɔ.
Chemotactic ƒe ŋusẽkpɔɖeamedzi ɖe dɔlélenutsiŋutete ƒe lãmenugbagbeviwo dzi: Kakɛ sia ɖea chemotactic ƒe ŋusẽkpɔɖeamedzi ɖe dɔlélenutsiŋutete ƒe lãmenugbagbeviwo hã dzi, si hea macrophages, neutrophils, kple dɔlélenutsiŋutete ƒe lãmenugbagbevi bubuwo yia teƒe siwo dɔdzẽ alo abi le. Macrophages wɔa akpa vevi aɖe le lãmenugbagbeviwo ɖɔɖɔɖo me, elabena woate ŋu akɔ lãmenugbagbevi siwo gblẽ kple dɔlékuiwo ɖa esime wole tsitsinu vovovowo kple cytokines ɖem ɖe go be woado lãmenugbagbeviwo ɖɔɖɔɖo kple wo gbugbɔgadzɔ ɖe ŋgɔ. TB 500 Fragment (17–23) blana ɖe xɔla tɔxɛ siwo le dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe anyime ŋu, si wɔnɛ be lãmenugbagbeviwo me dzesimɔwo wɔa dɔ be woafia mɔ dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ayi afisi woxɔ abi le.
(2) Lãmetsiwo Dzadzraɖo Kple Wo Gbugbɔgadzɔ
Fibroblast ƒe dzidziɖedzi kple collagen ƒe wɔwɔme dodo ɖe ŋgɔ: Fibroblasts nye lãmenugbagbevi ƒomevi vevitɔ le kadodoŋusẽ me eye woƒe dɔe nye be wowɔa lãmenugbagbeviwo godo ƒe matrix ƒe akpawo abe collagen ene heɖea wo ɖa le abi ƒe dɔyɔɣi. TB 500 Fragment (17–23) ateŋu aʋã fibroblast ƒe dzidziɖedzi, ana lãmenugbagbeviwo ƒe xexlẽme nadzi ɖe edzi eye wòato esia me ana collagen ƒe wɔwɔme nawɔ kabakaba. Kakɛ sia ate ŋu adzi domenyiŋusẽfianu siwo do ƒome kple kolagen ƒe wɔwɔme, abe COL1A1 kple COL3A1 ene ƒe ɖeɖefia ɖe dzi, esime wòdoa kolagen ƒe tɔtrɔ le gbegɔmeɖeɖe megbe ɖe ŋgɔ le fibroblasts me, si ana kolagen ƒe nyonyome kple eƒe liƒo nanyo ɖe edzi. Nusiwo dona tso eme siawo kpena ɖe lãmenugbagbeviwo godo ƒe ƒuƒoƒo sesẽ aɖe ƒe wɔwɔme ŋu, si doa abi ƒe dɔyɔyɔ kple lãmenugbagbeviwo ɖɔɖɔɖo ɖe ŋgɔ.
Lãmetsiŋusẽ ƒe lãkusiwo dzadzraɖo: Le lãmekawo ƒe abi dzadzraɖo me la, TB 500 Fragment (17–23) hã wɔa akpa vevi aɖe. Edoa satellite cells, siwo nye myogenic stem cells le ƒumeŋuɖui ƒe lãmeka me siwo si ŋutete le be woawɔ wo ɖokui yeyee ahato vovo azu lãmekawo ƒe kawo, ƒe dɔwɔwɔ kple woƒe dzidziɖedzi ɖe ŋgɔ. TB 500 Fragment (17–23) ɖɔa MyoD-Myogenin ƒe dzesimɔ si le satellite lãmenugbagbeviwo me ɖo, si doa woƒe vovototodedeameme ɖe ŋgɔ zua myotubes, siwo emegbe woƒoa ƒu wɔa lãmeka ƒe ka yeyewo, si wɔnɛ be lãmekawo ƒe lãmenugbagbeviwo ɖɔɖɔɖo kple wo gbugbɔgadzɔ. Kakɛ sia ate ŋu aɖe dɔdzẽ ƒe ŋuɖoɖowo dzi akpɔtɔ le lãmekawo ƒe abixɔxɔ vɔ megbe hã, si aɖe nusiwo agblẽ le lãmekawo ƒe lãkusiwo ŋu si dɔdzẽ hena vɛ dzi akpɔtɔ.
(3) Dɔlékuiwutikewo ƒe ŋusẽkpɔɖeamedzi
Mɔxexe ɖe dɔdzẽ ƒe nuwɔna ƒe ɖeɖefia nu: TB 500 Fragment (17–23) ate ŋu axe mɔ ɖe dɔdzẽ ƒe nu vovovowo ƒe ɖeɖefia nu, siwo dometɔ aɖewoe nye tumor necrosis factor-α (TNF-α) kple interleukin-1β (IL-1β). Dɔdzẽ siawo wɔa akpa vevi aɖe le dɔdzẽ ƒe ŋuɖoɖowo me, eye woƒe ɖeɖefia fũ ate ŋu ana dɔdzẽa nadzi ɖe edzi eye wòanɔ anyi, si ana lãmenugbagbeviwo nagblẽ. TB 500 Fragment (17–23) ateŋu aɖe dɔdzẽ ƒe nuwɔna ƒe nuŋɔŋlɔ kple gbegɔmeɖeɖe dzi akpɔtɔ to mɔxexe ɖe nuklia factor-κB (NF-κB) ƒe dzesimɔ ƒe dɔwɔwɔ nu, si ana dɔdzẽ ƒe ŋuɖoɖoa dzi naɖe akpɔtɔ. NF-κB nye nuŋɔŋlɔ ƒe nu vevi aɖe si le dɔdzẽ ƒe dzesiwo ƒe tɔtrɔ ƒe taɖodzinu vevitɔ, eye eƒe dɔwɔwɔ ate ŋu ana domenyiŋusẽfianu geɖe siwo do ƒome kple dɔdzẽ ƒe ɖeɖefia nadzi ɖe edzi.
Dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe dɔdzẽ ƒe ŋuɖoɖo dzi kpɔkpɔ: Tsɔ kpe ɖe mɔxexe ɖe dɔdzẽ ƒe nusiwo woɖena fiana nu ŋu la, TB 500 Fragment (17–23) ate ŋu aɖɔ dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe dɔdzẽ ƒe ŋuɖoɖo hã ɖo. Ate ŋu axe mɔ na macrophages ƒe polarization ɖe pro-inflammatory type (M1 ƒomevi) gbɔ eye wòado woƒe polarization ɖe anti-inflammatory type (M2 type) ŋu ɖe ŋgɔ. M1-ƒomevi macrophages koŋ ɖea nusiwo doa alɔ dɔdzẽ, kpɔa gome le dɔdzẽ ƒe gɔmedzedze kple edziyiyi me; ke M2-ƒomevi ƒe macrophages ya ɖea nusiwo tsia dzodzodzoetsitsi nu doa goe, si doa lãmenugbagbeviwo ɖɔɖɔɖo kple dɔlélenutsiŋutete ƒe ɖoɖowɔwɔ ɖe ŋgɔ. TB 500 Fragment (17–23) ɖɔa macrophages ƒe polarization nɔnɔme ɖo, si fiaa mɔ dɔdzẽ ƒe ŋuɖoɖoa yia mɔ si nyo na lãmenugbagbeviwo ɖɔɖɔɖo gbɔ.
TB 500 Fragment (17–23) zazã le Atikewɔwɔ me
(1) Abi ƒe Dɔyɔyɔ
Ŋutilã ƒe abiwo: Le ŋutigbalẽ ƒe abidada me la, TB 500 Fragment (17–23) ɖea dodoɖeŋgɔ ƒe ŋusẽkpɔɖeamedzi veviwo fiana. Numekuku siwo wowɔ do ŋgɔ na dɔdamɔnua ɖee fia be atike siwo me TB 500 Fragment (17–23) le zazã ɖe lãme nana abi ƒe tutu kabakaba eye wònaa dɔyɔyɔ ƒe nyonyome nyona ɖe edzi. Esia tso eƒe ɖoɖowɔwɔ ƒe ŋusẽkpɔɖeamedzi vovovowo ɖe lãmenugbagbevi siwo le lãkusi me, fibroblasts, kple dɔlélenutsiŋutete ƒe lãmenugbagbeviwo dzi gbɔ koŋ. Lãmenugbagbevi siwo le lãmenugbagbeviwo me ƒe ʋuʋu kabakaba doa ʋuka yeyewo ƒe wɔwɔ ɖe ŋgɔ, si naa nunyiame si sɔ abi la; fibroblast ƒe dzidziɖedzi kple collagen ƒe wɔwɔme wɔnɛ be kadodoŋusẽ sesẽ wu nɔa abixɔƒea; eye dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe atikewɔwɔ kple dɔlékuiwutikewo ɖea dɔdzẽ ƒe ŋuɖoɖowo ƒe mɔxexeɖedɔléle nu ƒe ŋusẽkpɔɖeamedzi ɖe abi ƒe dɔyɔyɔ dzi dzi kpɔtɔna. Le abi aɖewo siwo nɔa anyi didi, abe suklidɔ ƒe afɔbidɛwo ene dada me la, TB 500 Fragment (17–23) hã ɖea asixɔxɔ si ate ŋu anɔ eŋu le atikewɔwɔ me fiana. Suklidɔlélawo kpɔa abi ƒe hayahaya megbe eye dɔlékuiwo xɔxɔ ƒe afɔku dzina ɖe edzi le sukli ƒe agbɔsɔsɔ si le ʋu me ta. TB 500 Fragment (17–23) ateŋu ana nutoa me ƒe nuto suewo nanyo ɖe edzi, ado lãmenugbagbeviwo ƒe ʋuʋu kple dzidziɖedzi ɖe ŋgɔ, eye wòana abi siwo nɔa anyi didi ƒe dɔyɔyɔ ƒe mɔnukpɔkpɔ nadzi ɖe edzi.
Lãmetsiwo ƒe abi siwo le ememe: Le lãmeka siwo le eme ƒe abiwo, abe dɔgbo ƒe abi kple aklã ƒe gbegblẽ ene gome la, TB 500 Fragment (17–23) hã ate ŋu anye atikewɔwɔ ƒe ŋusẽkpɔɖeamedzi. Le dɔgbo ƒe abi ƒe kpɔɖeŋu me la, TB 500 Fragment (17–23) dodo doa lãmenugbagbevi siwo le lãkusi me ƒe dzidziɖedzi kple wo dzadzraɖo ɖe ŋgɔ le abi ƒe teƒea, si ɖea abi ƒe teƒe dzi kpɔtɔna. Eƒe dɔwɔwɔ ƒe mɔnu do ƒome kple lãmenugbagbeviwo ƒe ʋuʋu dodo ɖe ŋgɔ, dɔdzẽ ƒe ŋuɖoɖowo dzi kpɔkpɔ, kple lãmenugbagbeviwo godo ƒe matriki ƒe wɔwɔme dzi ɖeɖe kpɔtɔ. Le aklã ƒe abixɔxɔ ƒe kpɔɖeŋu me la, TB 500 Fragment (17–23) ate ŋu ado aklã me lãmenugbagbeviwo ƒe gbugbɔgadzɔ ɖe ŋgɔ, aɖe dɔdzẽ ƒe nugbegblẽ le aklã ƒe lãkusiwo ŋu dzi akpɔtɔ, eye wòana aklã ƒe dɔwɔwɔ ƒe dzesiwo nanyo ɖe edzi.
(2) Lãmetsiŋusẽdɔlélewo Dada
Lãmetsiwo ƒe tete kple abixɔxɔ: Le kamedefefewo ƒe atikewɔwɔ me la, lãmekawo ƒe tete kple abixɔxɔ nye nya siwo bɔ. TB 500 Fragment (17–23), le eƒe ɖɔɖɔɖoŋusẽ si wòkpɔna ɖe lãmekawo dzi ta la, do abe atike si ate ŋu ada lãmekawo ƒe abixɔxɔ ene. TB 500 Fragment (17–23) dodo le lãmeka ƒe tete megbe ate ŋu ana lãmeka ƒe ŋusẽ nagaɖo kabakaba eye wòaɖe lãmeka ƒe fibrosis ƒe dzɔdzɔ dzi akpɔtɔ. Lãmetsiŋusẽ ƒe ʋuʋu nye kuxi si bɔ ɖe lãmekawo ƒe abixɔxɔ megbe, si wɔnɛ be lãmekawo sesẽna, woƒe lãmekawo megate ŋu nɔa te o, eye lãmekawo ƒe dɔwɔwɔ megawɔa dɔ nyuie o. TB 500 Fragment (17–23) doa satellite cells ƒe dɔwɔwɔ kple woƒe dzidziɖedzi ɖe ŋgɔ, xea mɔ na dɔdzẽ ƒe ŋuɖoɖowo, eye wòɖea lãmekawo ƒe fibrosis ƒe wɔwɔ dzi kpɔtɔna, si wɔnɛ be lãmekawo ƒe abixɔxɔwo dzadzraɖo dzina ɖe edzi.
Ƒumeŋuɖui: Ƒumeŋuɖui nye ƒunukpeƒetetedɔ si bɔ si gblẽa nu le ƒunukpeƒewo ŋu, ƒunukpeƒetetedɔ, ƒunukpeƒetetedɔ, kple ƒuwo ƒe dzidziɖedzi. TB 500 Fragment (17–23) hã ɖee fia be ŋutete le ƒumeŋuɖui dada me. Ate ŋu ado chondrocytes ƒe dzidziɖedzi kple matrix synthesis ɖe ŋgɔ, axe mɔ na chondrocyte apoptosis, si ana ƒunukpeƒewo ƒe gbegblẽ nagbɔdzɔ. TB 500 Fragment (17–23) ƒe dɔlékuiwutikewo ate ŋu aɖe ƒunukpeƒetetedɔ dzi akpɔtɔ, aɖe ƒunukpeƒeveame dzi akpɔtɔ, eye wòaɖe abi dzi akpɔtɔ. Lãwo ŋuti numekukuwo ɖee fia be TB 500 Fragment (17–23) dodo ɖe ƒunukpeƒewo me ate ŋu ana ƒunukpeƒetetedɔ ƒe dɔwɔwɔ nanyo ɖe edzi le ƒumeŋuɖui ƒe kpɔɖeŋu lãwo me eye wòaɖe ƒunukpeƒetetedɔ ƒe gbegblẽ dzi akpɔtɔ.
(3) Dzitodzito me dɔlélewo dada
Dzitodzito me ʋu ƒe sisi: Dzitodzito me dɔléle nye dzitodzito me dɔléle sesẽ aɖe si tsoa dzitodzito me ʋu ƒe sisi kple necrosis le dzitodzito me ʋukawo ƒe xexe ta. TB 500 Fragment (17–23) ƒe asixɔxɔ ate ŋu azã le dzitodzito me dɔléle dada me. Le dzitodzito ƒe ʋuʋu megbe la, dzitodzito me lãkusiwo toa dɔdzẽ ƒe ŋuɖoɖowo kple lãmenugbagbeviwo ƒe tsɔtsrɔ̃ me, si wɔnɛ be dzitodzito megawɔa dɔ nyuie o. TB 500 Fragment (17–23) ateŋu ana dzi ƒe dɔwɔwɔ nanyo ɖe edzi le dzitodzito ƒe ʋuʋu megbe to mɔxexe ɖe dɔdzẽ ƒe ŋuɖoɖowo nu, dzitodzito me lãmenugbagbeviwo ƒe tsɔtsrɔ̃ dzi ɖeɖe kpɔtɔ, kple ʋukawo ƒe dzɔdzɔ dodo ɖe ŋgɔ me. Numekukuwo ɖee fia be le lãwo ƒe dzitodzito ƒe kpɔɖeŋuwo me la, TB 500 Fragment (17–23) zazã ate ŋu aɖe dzitodzito ƒe lolome dzi akpɔtɔ, ana dzitodzito ƒe dodo ƒe akpa sue aɖe nanyo ɖe edzi, eye wòana dzi ƒe tete kple ɖiɖiɖeme ƒe dɔwɔwɔ nanyo ɖe edzi.
Ʋukawo ƒe abi dzadzraɖo: Le ʋukawo ƒe abixɔxɔ, abe ʋukawo ƒe gbegblẽ le ʋukawo ƒe nudede dɔa me megbe ene la, TB 500 Fragment (17–23) ate ŋu ado ʋukawo ƒe lãmenugbagbeviwo ƒe ʋuʋu kple dzidziɖedzi ɖe ŋgɔ, ana ʋukawo ƒe ɖɔɖɔɖo kabakaba, eye wòaɖe afɔku si le ʋuka me ʋu ƒe sisi me dzi akpɔtɔ. Le ʋukawo ƒe abixɔxɔ megbe la, collagen si woɖe ɖe go le ʋumenugbagbeviɣia te ate ŋu ana ʋumenugbagbevi dzĩwo nalé ɖe wo nɔewo ŋu eye woaƒo ƒu, si ana ʋumenugbagbeviɣidɔ nadzɔ. TB 500 Fragment (17–23) doa endothelial cell ɖɔɖɔɖo ɖe ŋgɔ, si gbugbɔa ʋukawo ƒe endothelial ƒe blibonyenye ɖoa anyi, si wɔnɛ be thrombus ƒe wɔwɔme dzi ɖena kpɔtɔna eye wòkpɔa ʋukawo ƒe dɔwɔwɔ nyuie ta.
Nyanuwuwuw
TB 500 Fragment (17–23), abe peptide fragment si si nugbagbewo ƒe dɔwɔwɔ tɔxɛ le ene, ɖea dɔwɔwɔ nyuie fiana le lãmenugbagbeviwo ɖɔɖɔɖo, lãmenugbagbeviwo ƒe ʋuʋu, kple nu bubuwo me.
Dzɔtsoƒewo
[1] Esposito S, Deventer K, Goeman J, kple ame bubuwo. Thymosin beta 4 ƒe N-terminal acetylated 17-23 kakɛ si wokpɔ le TB-500, si nye atike si wosusu be atike muamewo zazã ƒe ŋutete le esi me ƒe wɔwɔme kple eƒe nɔnɔmewo ɖeɖefia[J]. Atikewo Dodokpɔ Kple Wo Me Dzodzro, 2012, 4 (9): 733-738.DOI: 10.1002 / dta.1402.
Adzɔnu si li na numekuku zazã ɖeɖeko :
