By Cocer Peptides 
1 ọnwa gara aga
Edemede na ozi ngwaahịa niile enyere na webụsaịtị a bụ naanị maka mgbasa ozi na ebumnuche mmụta.
Ngwaahịa ndị enyere na webụsaịtị a bụ naanị maka nyocha in vitro. A na-eme nyocha in vitro (Latin: * na iko *, nke pụtara na ihe eji enyo) na mpụga ahụ mmadụ. Ngwaahịa ndị a abụghị ọgwụ, ndị US Food and Drug Administration (FDA) akwadoghị ya, ha agaghịkwa eji ya gbochie, gwọọ, ma ọ bụ gwọọ ọnọdụ ahụike ọ bụla, ọrịa, ma ọ bụ ọrịa. Iwu amachibidoro nke ọma iwebata ngwaahịa ndị a n'ime ahụ mmadụ ma ọ bụ anụmanụ n'ụdị ọ bụla.
Okwu mmalite
TB 500 (Thymosin Beta-4 Fragment 17-23) bụ iberibe bioactive jikọtara ya na ọtụtụ isi usoro na physiology mmadụ, karịsịa na nrụzi anụ ahụ na mbugharị cell.
Ọgụgụ 1 Ọdịdị kemịkal nke TB 500 Iberibe (17-23).
Ozi bụ isi gbasara Iberibe TB 500 (17-23)
Iberibe TB 500 (17–23) bụ Iberibe N-terminal acetylated 17–23 nke thymosin beta-4 (Thymosin Beta-4). N-terminal acetylation na-arụ ọrụ dị oke mkpa n'ịhazi mmekọrịta protein-protein na mpaghara intracellular. Ọnụnọ nke otu acetyl nwere ike gbanwee nkesa ụgwọ molekụla na ngbanwe oghere, si otú ahụ na-emetụta ikike ya ijikọta na biomolecules ndị ọzọ. N'ime ahụ mmadụ, thymosin β4 bụ peptide na-ekesa nke ọma, yana TB 500 Fragment (17-23), dị ka akụkụ ya kpọmkwem, nwere ike ịmepụta site na usoro proteolytic endogenous.
Usoro nke omume nke iberibe TB 500 (17–23)
(1) Mbugharị cell na Chemotaxis
Mmetụta na mkpụrụ ndụ endothelial: TB 500 Fragment (17-23) na-akwalite mmegharị cell endothelial, usoro dị oke mkpa maka angiogenesis. N'oge ọgwụgwọ ọnya ma ọ bụ nrụzi anụ ahụ, mkpụrụ ndụ endothelial ga-akwaga na saịtị ndị mebiri emebi iji mepụta netwọk vaskụla ọhụrụ, iji nye oxygen na nri na anụ ahụ. TB 500 Fragment (17-23) nwere ike ịkwalite mbugharị cell endothelial site n'ịkwalite usoro ụzọ mgbaàmà intracellular, dị ka ụzọ akara PI3K-Akt, nke na-achịkwa nhazigharị nke cytoskeleton cellular. Ịkwalite ụzọ akara ngosi PI3K-Akt na-akwalite polymerization na depolymerization nke actin, na-eme ka mkpụrụ ndụ nwee ike ịgbatị pseudopodia na ịga n'ihu.
Mmetụta chemotactic na sel ndị na-alụso ọrịa ọgụ: Iberibe a na-egosipụtakwa mmetụta chemotactic na sel ndị na-alụso ọrịa ọgụ, na-adọta macrophages, neutrophils, na sel ndị ọzọ na-alụso ọrịa ọgụ na saịtị nke mbufụt ma ọ bụ mmerụ ahụ. Macrophages na-arụ ọrụ dị mkpa n'ịrụzi anụ ahụ, n'ihi na ha nwere ike ikpochapụ anụ ahụ mebiri emebi na ọrịa nje mgbe ha na-ezobe ihe dị iche iche na-eto eto na cytokines iji kwalite nrụzi anụ ahụ na ịmaliteghachi. Mpempe akwụkwọ TB 500 (17-23) na-ejikọta na ndị na-anabata ya kpọmkwem n'elu sel ndị na-alụso ọrịa ọgụ, na-eme ka ụzọ mgbaàmà intracellular na-eduzi sel ndị na-alụso ọrịa ọgụ na saịtị nke mmerụ ahụ.
(2) Mmezi anụ ahụ na mmụgharị
Na-akwalite mgbasawanye fibroblast na njikọ collagen: Fibroblasts bụ ụdị sel bụ isi na anụ ahụ jikọtara ya na ya na-ahụ maka ịmepụta na nzuzo nzuzo matrix extracellular dị ka collagen n'oge ọgwụgwọ ọnya. Iberibe TB 500 (17-23) nwere ike kpalite mmụba fibroblast, na-abawanye ọnụọgụ cell ma si otú a na-eme ka njikọ collagen dịkwuo elu. Iberibe a nwere ike imeziwanye okwu nke mkpụrụ ndụ ihe nketa metụtara njikọ njikọ collagen, dị ka COL1A1 na COL3A1, ebe ọ na-akwalite mgbanwe ntụgharị post-translational nke collagen n'ime fibroblasts, si otú ahụ na-emeziwanye ogo collagen na nkwụsi ike. Mmetụta ndị a na-enye aka n'ịmepụta matrix extracellular siri ike, na-akwalite ọgwụgwọ ọnya na nrụzi anụ ahụ.
Ndozi nke anụ ahụ: Na nrụzi mmerụ ahụ ahụ, TB 500 Fragment (17-23) na-arụkwa ọrụ dị mkpa. Ọ na-akwalite ịgbalite na mmụba nke sel satịlaịtị, nke bụ mkpụrụ ndụ sel myogenic na akwara skeletal nwere ike ime ka onwe ya dị ọhụrụ na ọdịiche dị n'ime eriri akwara. TB 500 Fragment (17-23) na-achịkwa ụzọ akara MyoD-Myogenin n'ime sel satịlaịtị, na-akwalite ọdịiche ha n'ime myotubes, nke na-ejikọta ya na-emepụta eriri anụ ahụ ọhụrụ, si otú ahụ nweta mmezi anụ ahụ na ịmaliteghachi. Iberibe a nwekwara ike ibelata nzaghachi mkpali mgbe mmerụ ahụ ahụ gasịrị, na-ebelata mmebi ọzọ nke anụ ahụ nke mbufụt kpatara.
(3) Mmetụta mgbochi mkpali
Mgbochi nke okwu mkpali: TB 500 Fragment (17-23) nwere ike igbochi nkwupụta nke ihe dị iche iche na-egbuke egbuke, gụnyere tumor necrosis factor-α (TNF-α) na interleukin-1β (IL-1β). Ihe ndị a na-egbuke egbuke na-arụ ọrụ dị mkpa na nzaghachi mkpali, na oke okwu ha nwere ike ime ka mmụba na nkwụsị nke mbufụt, na-akpata mmebi anụ ahụ. TB 500 Fragment (17-23) nwere ike ibelata nsụgharị na nsụgharị nke ihe ndị na-egbuke egbuke site n'igbochi ntinye nke ụzọ ihe mgbaàmà nuklia-κB (NF-κB), si otú ahụ na-ebelata nzaghachi mkpali. NF-κB bụ ihe dị mkpa ederede ederede na isi nke ntụgharị mgbaàmà mkpali, na ntinye ya nwere ike imeziwanye okwu nke ọtụtụ mkpụrụ ndụ ihe nketa metụtara mbufụt.
Ịchịkwa mmeghachi omume mkpali nke mkpụrụ ndụ na-alụso ọrịa ọgụ: Na mgbakwunye na igbochi nkwupụta nke ihe ndị na-akpata mkparị, TB 500 Fragment (17-23) nwekwara ike ịhazi mmeghachi omume mkpali nke mkpụrụ ndụ na-alụso ọrịa ọgụ. Ọ nwere ike igbochi polarization nke macrophages na ụdị pro-inflammatory (ụdị M1) ma kwalite polarization ha na ụdị mgbochi mkpali (ụdị M2). Ụdị macrophages nke M1 bụ isi na-ezobe ihe ndị na-akpata pro-inflammatory, na-ekere òkè na mmalite na mmụba nke mbufụt; ebe macrophages ụdị M2 na-ezochi ihe mgbochi mkpali, na-akwalite nrụzi anụ ahụ na nchịkwa mgbochi. Iberibe TB 500 (17-23) na-achịkwa ọnọdụ polarization nke macrophages, na-eduzi nzaghachi mkpali na ntụziaka dị mma maka ịrụzi anụ ahụ.
Ngwa nke TB 500 Fragment (17-23) na ngalaba ahụike
(1) Ọgwụgwọ ọnya
Ọnya akpụkpọ: Na ọgwụgwọ ọnya anụ ahụ, TB 500 Fragment (17-23) na-egosipụta mmetụta nkwado dị mkpa. Ọmụmụ ihe ọmụmụ izizi na-egosi na itinye n'usoro nke usoro nwere TB 500 Fragment (17-23) na-eme ka mmechi ọnya na-eme ka ọ dịkwuo mma. Nke a bụ n'ụzọ bụ isi n'ihi mmetụta nchịkwa ya dị iche iche na sel endothelial, fibroblasts, na sel ndị na-alụso ọrịa ọgụ. Mbugharị sel endothelial accelerated na-akwalite nhazi arịa ọbara ọhụrụ, na-enye nri zuru oke maka ọnya; mmụba nke fibroblast na njikọ collagen na-eme ka anụ ahụ dị ike na-ejikọta na saịtị ọnya; na mmetụta chemotactic na mgbochi mkpali nke sel ndị na-adịghị ahụkebe na-ebelata mmetụta mgbochi nke nzaghachi mkpali na ọgwụgwọ ọnya. N'ịgwọ ụfọdụ ọnya na-adịghị ala ala, dị ka ọnya ụkwụ na-arịa ọrịa shuga, TB 500 Fragment (17-23) na-egosipụtakwa uru ọgwụgwọ nwere ike. Ndị ọrịa na-arịa ọrịa shuga na-enweta ọgwụgwọ ọnya na-egbu oge yana ihe ize ndụ ibute ọrịa n'ihi mmụba shuga dị n'ọbara. Iberibe TB 500 (17-23) nwere ike imeziwanye microenvironment mpaghara, kwalite mbugharị cell na mmụba, ma kwalite ohere nke ịgwọ ọrịa na ọnya na-adịghị ala ala.
Ọnya anụ ahụ dị n'ime: Maka ọnya anụ ahụ dị n'ime, dị ka ọnya afọ na mmebi imeju, TB 500 Fragment (17-23) nwekwara mmetụta ọgwụgwọ. N'ụdị ọnyá afọ eriri afọ, nchịkwa nke TB 500 Fragment (17-23) na-akwalite mmụba na mmezi nke mkpụrụ ndụ mucosal na saịtị ọnya, na-ebelata mpaghara ọnyá afọ. Ejikọtara usoro ihe omume ya na ịkwalite mbugharị cell, na-achịkwa nzaghachi mkpali, yana ịba ụba njikọ matrix extracellular. Na ụdị mmerụ ahụ imeju, TB 500 Fragment (17-23) nwere ike ịkwalite imeju cell imeju, belata mmebi mmebi nke anụ ahụ imeju, ma melite ọrụ imeju.
(2) Ọgwụgwọ Ọrịa Musculoskeletal
Ahụhụ akwara na mmerụ ahụ: Na ọgwụ egwuregwu, akwara anụ ahụ na mmerụ ahụ bụ okwu nkịtị. Mpempe akwụkwọ TB 500 (17-23), n'ihi mmetụta nrụgharị ya na anụ ahụ akwara, apụtala dị ka onye nwere ike ịgwọ ọrịa maka mmerụ ahụ ahụ. Nlekọta nke TB 500 Fragment (17-23) na-eso ụdị anụ ahụ nwere ike ime ka mgbake nke ike anụ ahụ dịkwuo ngwa ma belata ihe omume fibrosis muscle. Fibrosis muscle bụ ihe mgbagwoju anya na-esochi mmerụ ahụ ahụ, na-eduga n'ịgbasi ike nke anụ ahụ, mbelata nrịda, na arụrụ arụ ọrụ. TB 500 Fragment (17-23) na-akwalite nkwalite na mmụba nke mkpụrụ ndụ satịlaịtị, na-egbochi nzaghachi mkpali, ma na-ebelata nhazi nke fibrosis muscle, si otú ahụ na-eme ka mmezi nke mmerụ ahụ dịkwuo mma.
Osteoarthritis: Osteoarthritis bụ ọrịa nkwonkwo na-akpatakarị mmebi nke cartilage, mbufụt synovial, na mmụba ọkpụkpụ. Iberibe TB 500 (17–23) egosiwokwa ike n'ịgwọ ọrịa ogbu na nkwonkwo. Ọ nwere ike ịkwalite mmụba na njikọ matrix nke chondrocytes, na-egbochi apoptosis chondrocyte, si otú a na-ebelata mmebi nke cartilage. Mmetụta mgbochi mkpali nke TB 500 Fragment (17-23) nwere ike ibelata mbufụt synovial, belata mgbu nkwonkwo, ma belata ọzịza. Ọmụmụ anụmanụ egosila na ịgba ọgwụ intra-articular nke TB 500 Fragment (17-23) nwere ike imeziwanye ọrụ nkwonkwo na ụdị anụmanụ osteoarthritis ma belata mmebi nke cartilage.
(3) Ọgwụgwọ ọrịa obi
Myocardial infarction: Myocardial infarction bụ ọrịa obi siri ike nke ischemia myocardial na necrosis kpatara n'ihi nkwụsị nke akwara obi. Iberibe TB 500 (17-23) nwere uru ngwa ngwa n'ịgwọ ọrịa myocardial myocardial. Na-esochi myocardial infarction, anụ ahụ myocardial na-enweta nzaghachi mkpali na cell apoptosis, na-eduga n'ịrụ ọrụ obi. Iberibe TB 500 (17-23) nwere ike imeziwanye ọrụ obi na-arụ ọrụ obi mgbe myocardial infarction site na igbochi nzaghachi mkpali, ibelata apoptosis cell myocardial, na ịkwalite angiogenesis. Nnyocha e mere egosiwo na n'ụdị anụmanụ nke ọrịa myocardial myocardial, iji TB 500 Fragment (17-23) nwere ike ibelata ogo infarct, meziwanye akụkụ nke nkwụsị obi obi, ma kwalite ọrụ obi na ntụrụndụ.
Ndozi mmerụ ahụ vaskụla: N'ọnọdụ nke mmerụ ahụ vaskụla, dị ka mmebi endothelial na-esote ntinye aka nke vaskụla, TB 500 Fragment (17-23) nwere ike ịkwalite mbugharị sel endothelial na mmụba, mee ngwa ngwa nrụzi endothelial, ma belata ohere nke ịmepụta thrombus. Na-esochi mmerụ ahụ endothelial vaskụla, collagen ekpughere n'okpuru endothelium nwere ike ịkpalite nchikota platelet na mkpokọta, na-eduga n'ichepụta thrombus. Mpempe akwụkwọ TB 500 (17-23) na-akwalite nrụzi mkpụrụ ndụ endothelial, na-eweghachi iguzosi ike n'ezi ihe nke endothelial vaskụla, si otú a na-ebelata nhazi thrombus ma na-echebe ọrụ vaskụla nkịtị.
Mmechi
Iberibe TB 500 (17-23), dị ka mpempe peptide nwere ọrụ ndu pụrụ iche, na-egosipụta nrụpụta ọrụ na nrụzi anụ ahụ, mbugharị cell, na mpaghara ndị ọzọ.
Isi mmalite
[1] Esposito S, Deventer K, Goeman J, et al. Nkwekọrịta na njiri mara nke N-terminal acetylated 17-23 nke thymosin beta 4 nke achọpụtara na TB-500, ngwaahịa a na-enyo enyo na ọ nwere ike doping[J]. Nyocha na nyocha ọgwụ, 2012,4 (9): 733-738.DOI: 10.1002 / dta.1402.
Ngwaahịa dị maka iji naanị nyocha :
