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1kits (Ebipipa 10) .
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▎ ya Cardiogen Enzimba
Ensibuko: PubChem |
Omutendera: AEDR Ensengekera ya molekyu: C 18H 31N 7O9 Obuzito bwa molekyu: 489.5 g/mol PubChem CID:11583989.Ekitongole ky’ebyobulamu Ebigambo ebifaanagana: SCHEMBL3194515 |
▎ ku Cardiogen Okunoonyereza
Kiki ekikoleddwa mu kunoonyereza ku Cardiogen?
Nga evugirwa ebyetaago by’okuziyiza/okujjanjaba ebizimba n’obujjanjabi bw’endwadde z’emisuwa, okunoonyereza ku Cardiogen kufunye okufaayo okunene. Abanoonyereza bakizudde nti Cardiogen peptide eziyiza transplantable M-1 sarcoma, okuleeta tumor cell apoptosis ne hemorrhagic necrosis, ekiwa obulagirizi obupya eri obujjanjabi bw’ekizimba. Mu kiseera kino, omugaso gwayo oguyinza okubaawo mu kulungamya emiwendo gy’omubiri gw’omutima n’okutumbula okuddamu okukola kw’emisuwa gy’omutima nagwo guleese emikisa emipya egy’okunoonyereza ku bujjanjabi bw’endwadde z’emisuwa.
Olw’okulinnya kw’okukola eddagala lya bioactive peptide, okunoonyereza ku nsengeka ya Cardiogen n’enkola yaayo kwa makulu nnyo nga peptide ekola mu biramu ng’erina eby’obugagga eby’enjawulo. Enkulaakulana ya tekinologiya nga genetic engineering ne proteomics ewadde obuyambi obw’ekikugu okunoonyereza okw’obwegendereza ku nsibuko ya Cardiogen n’okunnyonnyola enkola yaayo ey’okukola, okuvuga okunoonyereza okukwatagana nayo okugenda mu maaso.
Enkola ya Cardiogen ekola ki?
Induction of Tumor Cell Apoptosis: Okunoonyereza kulaga nti oluvannyuma lw’okukuba Cardiogen mu nvunza ezirina transplantable M-1 sarcoma, omutindo gw’okufa kw’obutoffaali bw’ekizimba mu bibinja byonna eby’okugezesa gwali waggulu okusinga mu kibinja ekifuga. Kino kiraga nti Cardiogen esobola okuleeta enkola z’okufa mu butoffaali bw’ekizimba okuyita mu makubo agamu, ekireetera obutoffaali bw’ekizimba okuyita mu kufa kw’obutoffaali okutegekeddwa. Apoptosis nkola obutoffaali mwe bumalira obulamu bwabwo nga bugoberera pulogulaamu zaabwo wansi w’embeera ezimu ez’omubiri oba ez’obulwadde. Mu budde obwabulijjo, apoptosis efugirwa bulungi omuddirirwa gw’obuzaale n’amakubo agalaga obubonero. Cardiogen eyinza okukola obuzaale obukwatagana n’okufa oba amakubo agalaga obubonero, gamba ng’okukola ku puloteyini z’amaka ga Bcl-2 okwongera okwolesebwa kwa puloteyina eziwagira apoptotic (okugeza, Bax) oba okuziyiza omulimu gwa puloteyina ezilwanyisa apoptotic (okugeza, Bcl-2), bwe kityo ne kireetera okufulumya cytochrome C okuva mu mitochondria mu cytoplasm n’okukola enkola ya cascade cascade, okukkakkana nga kivaako okufa kw’obutoffaali bw’ekizimba [1]. .
Ekifaananyi 1. Enkola z’obuzaale mu buzibu bw’emisuwa. Omutima (wakati) gukwatagana n’enkola ssatu enkulu ezifuga epigenetic ezikwata ku cardiovascular pathophysiology.
Ensibuko:MDPI [5] .
Induction of Tumor Hemorrhagic Necrosis: Okuziyiza okukula kwa M-1 sarcoma okusinziira ku ddoozi oluvannyuma lw’okukuba empiso ya Cardiogen kiva ku kitundu ku tumor hemorrhagic necrosis. Okukula kw’ekizimba kwesigamye ku neovascularization okusobola okufuna ebiriisa ne oxygen. Cardiogen eyinza okutuuka ku kikolwa kino nga ekosa enkola eya bulijjo ey’omukutu gw’emisuwa gy’ekizimba. Kiyinza okukola ku butoffaali bw’emisuwa gy’ekizimba, okutaataaganya obubonero bw’ensonga ezikwatagana n’okutondebwa kw’emisuwa (nga ensonga y’okukula kw’emisuwa VEGF), okutaataaganya obusobozi bw’okukula, okusenguka, n’okutondebwa kw’olubuto lw’obutoffaali bw’emisuwa, ekivaako okukosa okuzimba emisuwa gy’ekizimba, ischemia y’ekizimba mu kitundu, n’obutaba na mukka mu bitundu by’omubiri, n’okuleeta okuvaamu omusaayi obulwadde bw’okufa (necrosis). Mu kiseera kye kimu, kiyinza okwonoona butereevu emisuwa gy’omusaayi egy’ekizimba egiriwo, ne kivaako emisuwa okukutuka n’okuvaamu omusaayi, ne kyongera okuleetawo okufa kw’ebitundu by’ekizimba [1]..
Non-Direct Cytostatic Effect: Okusinziira ku ndowooza y’ebipimo by’emirimu gy’okusaasaana, okuziyiza okukula kw’ekizimba tekuva ku direct cytostatic effect ya Cardiogen ku butoffaali bw’ekizimba. Kino kitegeeza nti tekiziyiza butereevu nkola za musingi za mubiri nga okukola DNA y’obutoffaali bw’ekizimba oba okukola puloteyina ng’eddagala ery’ekinnansi eritta obuwuka okuziyiza okukula kw’obutoffaali bw’ekizimba. Wabula, kiziyiza okukula kw’ekizimba okuyita mu nkola ezitali butereevu nga okuleeta apoptosis n’okuleeta tumor hemorrhagic necrosis. Engeri eno ey’ekikolwa ekitali kya butereevu cytostatic eyinza okufuula Cardiogen okusobola okuba ey’obukuumi ng’obutwa butono nnyo eri obutoffaali obwa bulijjo ate nga ekola ebikolwa eby’okulwanyisa ebizimba [1]..
Action Through Tumor Vascular Network: Obujulizi obw’enkula bulaga nti ekikolwa kya Cardiogen kizingiramu enkola eyeetongodde okuyita mu mutimbagano gw’emisuwa gy’ekizimba. Omukutu gw’emisuwa gy’ekizimba tegukoma ku kuwa biriisa eri obutoffaali bw’ekizimba wabula era gukola kinene mu kusaasaana kw’ekizimba n’enkola endala. Cardiogen eyinza okwesiba ku bikwata ebitongole ku ngulu w’obutoffaali (nga obutoffaali bw’omubiri (endothelial cells) ne pericytes) mu mutimbagano gw’emisuwa gy’ekizimba, okukola amakubo g’okukyusa obubonero mu butoffaali n’okuleeta ebikolwa ebiwerako eby’ebiramu, gamba ng’okulungamya okuyita kw’emisuwa n’okukwata ku kukonziba n’okuwummuzibwa kw’obutoffaali bw’ebinywa ebiseeneekerevu, bwe kityo ne kikosa microenvironment y’ebitundu by’ekizimba n’ okukkakkana nga kikwata ku kukula kw’ekizimba, okufa kw’ekizimba, n’enkola endala.
Okugatta ku ekyo, ensengekera n’enkola etali ya bulijjo ey’omukutu gw’emisuwa gy’ekizimba biwa omusingi gw’ekikolwa kya Cardiogen ekigendereddwamu, ekigisobozesa okukola mu ngeri ey’enjawulo ku bitundu by’ekizimba nga tekirina kinene kye zikola ku misuwa gy’omusaayi egy’ebitundu ebya bulijjo [1].
Biki ebikozesebwa mu kukola Cardiogen?
Ebikosa ku bipimo ebikwatagana n’omutima: Mu mbeera z’okunyigirizibwa, Cardiogen ekola kinene mu kulungamya okukuba kw’omutima n’obuzito bw’ebitundu by’omubiri mu nvunza. Okunoonyereza kulaga nti empiso ya Cardiogen esobola okusasula ebikosa adrenaline ebiva ku situleesi ku nkyukakyuka z’obuzito bw’omutima, endwadde z’omusulo, n’ennywanto, ne kifuula obuzito bw’ebitundu bino mu mbeera eya bulijjo. Mu kiseera kye kimu, kiyamba okukendeeza ku kukuba kw’omutima okufuga emiwendo n’okukendeeza ku kwolesebwa kw’obutatambula bulungi mu situleesi. Kino kiraga nti Cardiogen esobola okukuuma embeera y’omubiri eya bulijjo ey’omutima n’ebitundu ebikwatagana nabyo mu mbeera ezirimu situleesi, ekiyamba obulungi mu kutebenkeza enkola y’emisuwa gy’omutima [2]..
Enkozesa mu Kujjanjaba Endwadde z’Omutima
Obujjanjabi bw’okusannyalala kw’emisuwa gy’omutima: Cardiogenin, ekitundu ekikola ekyawuddwa okuva mu bimera ebiva mu bimera (ekiyinza okuba nga kyekuusa ku Cardiogen oba ekimu ku birungo byayo ebikola), kiraga nti kikola bulungi mu bikolwa by’ebisolo eby’okusannyalala kw’omusuwa gw’omutima. Okunoonyereza kuzudde nti cardiogenin esobola nnyo okuddaabiriza emitima egya infarcted, nga endogenous mesenchymal stem cells (MSCs) etunuuliddwa nga zaawukana ne cardiomyocytes empya mu kitundu ekifunye infarcted, nga ziwerekerwako okulongoosa ennyo mu nkola y’omutima.
Okusimbuliza MSCs nga tezinnaba kujjanjabwa ne EGJ (ekirungo ekiva mu bimera ekirimu cardiogenin) oba cardiogenin mu bikolwa by’ebisolo ebizimba omusuwa gw’omutima nakyo kitumbula okuddamu okukola ennyo kw’omusuwa gw’omutima ogukola. Kino kiraga nti cardiogenin tekoma ku kuba na busobozi kutumbula kuddamu kukola misuwa gya mutima wabula esobola n’okukola MSCs, okuwa embeera ezeetaagisa okuddamu okukola emisuwa gy’omutima n’okuggulawo amakubo amapya ag’obujjanjabi bw’okuzimba emisuwa gy’omutima. Ekirala, okugaba cardiogenin mu kamwa nakyo kiragiddwa okuba n’ebikosa ebinene mu bujjanjabi ku myocardial infarction, nga endogenous MSCs ezisibuka mu busimu bw’amagumba zikakasiddwa ng’ensibuko y’obutoffaali obusookerwako obw’omusuwa gw’omutima oguzzeemu okutondebwa. Okunoonyereza okusooka okw’enkola kulaga nti miR-9 ne target yaayo E-cadherin eyinza okwenyigira mu kukola disiki eziyingiziddwa [3, 4]..
Okukozesebwa mu bujjanjabi bwa kookolo: Cardiogen peptide eraga ebikolwa ebikyusa ebizimba mu nvunza ezirina M-1 sarcoma eyasimbibwa. Okunoonyereza kulaga nti oluvannyuma lw’okukuba empiso ya Cardiogen, omutindo gw’okufa kw’obutoffaali bw’ekizimba mu bibinja byonna eby’okugezesa gwali waggulu okusinga mu kibinja ekifuga, era eddagala lino lyaziyiza okukula kwa M-1 sarcoma mu ngeri eyesigamiziddwa ku ddoozi. Kino ekiziyiza tekiva ku kikolwa ky’eddagala obutereevu eky’okuziyiza obutoffaali ku bizimba wabula okusinga ku kuleeta okufa kw’omusaayi mu bizimba n’okusitula okufa kw’obutoffaali bw’ekizimba. Mu nkula, Cardiogen eyinza okukozesa enkola yaayo entongole ey’okukola okuyita mu mutimbagano gw’emisuwa gy’ekizimba, okuwa enkola empya eyinza okujjanjaba kookolo nga etereeza microenvironment y’ekizimba n’okuleeta apoptosis y’obutoffaali bw’ekizimba [3, 4]..
Mu bufunzi
Mu bufunze, Cardiogen esobola okulungamya enkola y’emisuwa gy’omutima, okulongoosa enkola y’omutima n’ebitundu ebikwatagana nabyo nga bali ku situleesi, n’okutumbula okuddaabiriza okuzimba emisuwa gy’omutima. Mu kiseera kino, engeri yaayo eya peptide esobola okuleeta okufa kw’obutoffaali bw’ekizimba, okuleeta okufa kw’omusaayi, n’okuziyiza okukula kw’ekizimba, ne kiwa endagiriro empya ez’obujjanjabi bw’endwadde.
Ebikwata ku Muwandiisi
Ebintu ebyogeddwako waggulu byonna binoonyerezeddwako, birongooseddwa era ne bikung’aanyizibwa kkampuni ya Cocer Peptides.
Omuwandiisi wa Scientific Journal
Cheng, Lei mumanyi wa kitiibwa alina obumanyirivu bungi mu kunoonyereza mu ssaayansi w’obulamu ne ssaayansi w’ebintu. Nga yeegasse ku matendekero ag’ettutumu nga Hong Kong Polytechnic University, Dali University, ne Chinese University of Hong Kong, alaze nti alina obumanyirivu obw’enjawulo era obw’obuziba mu by’ensoma. Okunoonyereza kwe kukwata ku Biochemistry & Molecular Biology, Cell Biology, ne Materials Science, eziri ku mwanjo mu kunoonyereza kwa ssaayansi okw’omulembe era nga zirina obusobozi obw’amaanyi obw’okukozesa. Omulimu gwa Cheng, Lei tegussa essira ku kunoonyereza kwa ssaayansi okw’omusingi kwokka wabula n’okugatta ssaayansi ne tekinologiya, okuwa obuwagizi obw’amaanyi eri enkulaakulana y’enzikiriziganya n’obuyiiya bwa tekinologiya mu bintu ebikwatagana nabyo. Kaweefube we ow’okukolagana n’amatendekero ag’enjawulo naye abadde mukulu nnyo mu kutumbula okuwanyisiganya eby’ensoma n’enkolagana mu kunoonyereza.. Cheng, Lei is listed in the reference of citation [3].
