LL-37 miligaramu 5

▎ LL-37 Jatebɔcogo

Sɔrɔyɔrɔ: PubChem

A kɛcogo: LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES Formula molekilɛri: C 205H 340N 60O53 Molecules girinya: 4493 g/mol CAS nimɔrɔ: 154947-66-7 PubChem ka CID: 16198951. Bamako, Mali Kɔrɔɲɔgɔnmaw: Cathelicidin;ropocamptide

▎ LL-37 Sɛgɛsɛgɛli

LL-37 ka ɲininiw kɛcogo ye mun ye?

LL-37 sɔrɔla fɔlɔ i n’a fɔ katiyɔni pepitiri misɛnninw kulu dɔ siliki kɔnɔ Samia cynthia ricini ka pupew kɔnɔ Suwɛdi dɔnnikɛla Boman HG fɛ san 1980. LL-37 ye hadamaden ka katelisidini banakisɛfagalan pepitiri C-terminal peptide (CAMP, hCAP18) ye, min bɛ se ka dɔ fara a ka se ka banakisɛfagalanw kɛlɛ binkanni ani a bɛ farikoloɲɛnajɛ baara nafamaw kɛ kemikorosi la, ka joginda datuguli sabati, ani joli-sira-funu (Chen X, 2018). Banakisɛfagalanw bɛ sɔrɔ kosɛbɛ baganw, jiriw ani fɛnɲɛnama misɛnnin dɔw la, wa u ye kolotugudaw ka farikolo tangacogo bangenen yɔrɔ nafamaba dɔ ye. I n’a fɔ gundolafɛn, LL-37 bɛ sɔrɔ kosɛbɛ hadamaden farikolo yɔrɔ caman na ani a farikolo yɔrɔ caman na. Kɔnɔnatumu suguya caman, i n’a fɔ ɲɛgɛnɛsiralabanaw, keratinocytes, mast cellules, neutrophils, macrophages ani monocytes, olu bɛ se k’a bɔ. Nin banakisɛfagalan suguya fanba bɛ 'amino acid residues' 37 - 39 sɔrɔ ani sitɛyini 0 bɛ a la. K’a sababu kɛ basigi barikama ye banakisɛfagalan pepitiri ka N-terminal jɔyɔrɔ la, a bɛ se ka 'anphiphilic helical structure' sabatilen dilan. Banakisɛfagalan pepitiri LL-37 fana bɛ ni 'anfifili α-helical structure' ye. K’a sababu kɛ a ka baara ye ka banakisɛw faga, a tɔgɔ dara ko banakisɛfagalan pepitiri. '37' min bɛ LL-37 tɔgɔ la, o bɛ se ka kɛ a ka amino asidi tolenw hakɛ ye. O waati kelen na, a bɛ fɔ fana ko katelisidini ni ropokamptidi.

LL-37 ka baara kɛcogo ye mun ye banakisɛfagalanw kɛlɛli la?

Banakisɛw ka selilɛriw tiɲɛni: 

LL-37 bɛ se ka don banakisɛw ka selilɛri kɔnɔ, kɛrɛnkɛrɛnnenya la, a bɛ se ka tiɲɛni kɛ selilɛri la, fosifatidiligilisirɔli (DPPG) bɛ min na. A bɛna banakisɛw ka selilɛriw jɔcogo tiɲɛ, o la, a bɛ banakisɛfagalan nɔ bila ^[1] . Misali la, sɛgɛsɛgɛliw y’a jira ko LL-37 bɛ se ka don banakisɛ Gram-positif ni Gram-negatif fila bɛɛ ka selilɛriw kɔnɔ, o bɛ na ni dɔ farali ye selilɛri ka doncogo kan, ka selilu kɔnɔfɛnw wuli, ka laban ka banakisɛw faga.

Banakisɛfagalanw ka baara kɛcogo min ka bon kosɛbɛ: 

LL-37 bɛ banakisɛfagalanw kɛ banakisɛ suguya caman na minnu bɛ se ka banakisɛfagalanw kɛlɛ. A bɛ se ka banakisɛw kɛlɛ minnu bɛ wele ko Gram-positif (i n’a fɔ Staphylococcus aureus, Streptococcus, Enterococcus, a ɲɔgɔnnaw), Gram-negatif banakisɛw (i n’a fɔ Pseudomonas aeruginosa, Escherichia coli, Salmonella, a ɲɔgɔnnaw), ani banakisɛ wɛrɛw (i n’a fɔ Mikoplasma, Ureaplasma, Mycobacterium, a ɲɔgɔnnaw) ^[2] . Nin banakisɛfagalan baara min bɛ kɛ ka caya, o b’a to LL-37 bɛ se ka kɛ sababu ye ka banakisɛfagalanw kɛlɛ banakisɛ suguya wɛrɛw kɛlɛli la.

Biyofilimu min dilannen don, o tiɲɛni: 

Banakisɛw ka biyofilimu ye sababu nafama dɔ ye banakisɛw ka fura tali la. Banakisɛfagalan pepitiri LL-37 bɛ se ka biyofilimu dilannen tiɲɛ, o la, a bɛ dɔ bɔ banakisɛw ka fura tali la. Misali la, kolotugudaw banakisɛfagalanw (PJI) la, kolotugudaw caman cili kɔfɛ, banakisɛw ka fura tali min bɛ sɔrɔ banakisɛw ka biyofilimu fɛ, o bɛ furakɛli gɛlɛya. Nka, LL-37 bɛ se ka jɔyɔrɔ ɲuman ta banakisɛfagalanw ni banakisɛfagalanw na ni a bɛ biyofilimu sɔrɔli bali ani ka biyofilimu dilannen tiɲɛ.

Banakisɛfagalanw ka banakisɛfagalanw baara sabatili : 

Kɔlɔsiliw y’a jira ko LL-37 bɛ se ka baara kɛ ɲɔgɔn fɛ ni banakisɛfagalan dɔw ye. Misali la, ni a ni asidi amɔkisisilini klavulaniki (AMC) faralen don ɲɔgɔn kan, LL-37 bɛ se ka AMC ka banakisɛfagalanw baara fanga bonya kosɛbɛ.

Soso:PubMed ^{[6] Ɲɛjirali dɔw.}

LL-37 ka baarakɛminɛnw ye mun ye?

Kolow labɛnni sabatili sabatili: 

Kalan dɔw y’a jira ko banakisɛfagalan pepitiri LL-37 bɛ nɔ ɲuman bila kolo labɛnni na. Sɛgɛsɛgɛli dɔw y’a jira ko hadamadenw ka tulumafɛnw (hADSCs) lamɔna ni LL-37 hakɛ danfaralenw ye, wa a jirala ko LL-37 hakɛ ye nɔ bila hADSCw koloci seko la, ka se a dan na ni 4μg/ml ye. Ka fara o kan, PSeD/hADSCs/LL-37 faralen ɲɔgɔn kan sɛgɛsɛgɛlikɛlan ye koloci kɛcogo ɲuman jira ka tɛmɛ PSeD/hADSCs, PSeD ani kɔrɔsili kulu sɛgɛsɛgɛlikɛlanw kan sosow ka wuluwulu nafama modɛli la, o b’a jira ko sebaayaba bɛ a la kɛnɛyaso kolo lasegincogo la.

Banakisɛfagalanw nɔ:

Banakisɛ caman balili: 

Sɛgɛsɛgɛli dɔw kɛra ni micro-double dilution fɛɛrɛ ye walasa ka banakisɛfagalan pepitiri LL-37 hakɛ dɔgɔyalenba (MIC) dɔn Escherichia coli, Salmonella ani Staphylococcus aureus kɛlɛli la. Jateminɛw y’a jira ko LL-37 ka balili hakɛ tɛ kelen ye nin banakisɛ saba ninnu na, ni balili hakɛ fitinin ye 3,12, 1,56 ani 0,78μg/mL ye. Sumaya sabatili sɛgɛsɛgɛli y’a jira ko banakisɛfagalan pepitiri min bɛ ɲɔgɔn sɔrɔ kokura, o tun bɛ baara ɲuman kɛ hali bi funteni caman na. Asidi-basi sabatili sɛgɛsɛgɛli jaabiw y’a jira ko LL-37 bɛ baara dɔw kɛ ni pH ye 2,0 fo 12,0 ye, ni baara ɲuman ye pH 5,0 fo 6,0 la, ani -20°C ye cogoya ɲuman ye min bɛ se ka mara waati jan ^kɔnɔ.

A bɛ nɔ min bila banakisɛfagalanw tali la: 

Mɔgɔ dɔw ye banakisɛfagalan pepitiri LL-37 ni warijɛ nanoparticules (AgNPs) ka banakisɛfagalanw nafa sɛgɛsɛgɛ Staphylococcus aureus (S. aureus) kan, o ye fɛnɲɛnama fitinin ye min bɛ sɔrɔ ka caya banakisɛw la minnu bɛ sɔrɔ biyofilimu fɛ. Jateminɛw y’a jira ko LL-37 de ye banakisɛfagalan ye min bɛ se ka kɛ kosɛbɛ, ni koloni hakɛ dɔgɔyara ka tɛmɛ logaritmu 4 kan. O ni warijɛ nanofɛnw ni banakisɛfagalan kɔrɔw nɔ tun man ɲi, ni koloni hakɛ dɔgɔyara ka tɛmɛ logaritmu 1 kan. Banakisɛfagalanw faralen ɲɔgɔn kan furakɛli ni rifampisini ye, o ye dɔ fara kosɛbɛ AgNPw ni jentamisini dɔgɔyali logaritmi fɛ, nka hali bi a tun ka dɔgɔ kosɛbɛ ka tɛmɛ LL-37 ta kan min kɛra a kelen ^na.

A kɛcogo fogonfogonlabana na: 

Kɔlɔsiliw y’a jira ko Pseudomonas aeruginosa (PA) kɛra gɛlɛya kɔrɔtɔlen ye fogonfogonlabana ni fogonfogon joginni na. LL37 pepitiri ye banakisɛfagalan ɲuman ye PA suguyaw kama, nka a tali dan ye ka da a bɔli teliya kan in vivo, biyosafety koɲɛw, ani bioavailability dɔgɔyali. O la, nanofura dicogo dɔ labɛnna min sinsinnen bɛ albumini kan, min bɛ se ka dɔgɔya, walasa ka LL37 ka baara kɛcogo ɲɛ ka ɲɛsin PA ma in vivo, ni disulfide jɛɲɔgɔnyaw kɛli ye molekiyɔmuw ni ɲɔgɔn cɛ. Katiyɔniki LL37 bɛ se ka don a kɔnɔ ka ɲɛ ni kuran ye walasa ka banakisɛfagalanw kɛcogo ɲuman jira. LL37 pepitiri ye bɔli sabatilen jira ka tɛmɛ sanga 48 kan ka bɔ LL37 pepitiri nanoparticules (LL37 PNP) la, wa banakisɛfagalanw nɔ janyalen kɔlɔsira ni dɔ farala a kɔnɔ waati kan. PA fogonfogonlabana jugumanba sosow ka misali la, LL37 PNP ye TNF-α ni IL-1β jirali dɔgɔya kosɛbɛ ani ka fogonfogon joginni nɔgɔya. A b’a jira ko LL37 PNP bɛ se ka PA fogonfogonlabana ni a kɔfɛ funu jaabi ɲɛ ka tɛmɛ LL37 pepitiri hɔrɔnma kan ^[3]..

Ka joli segin furakisɛw ka banakisɛfagalanw baara baara kɛ: 

Kɔlɔsiliw y’a jira ko banakisɛfagalan pepitiri LL-37 bɛ se ka hadamadenw ka joli segin furakisɛw ka banakisɛfagalanw baara kɛ. Ni joli segin furakisɛw furakɛra ni LL-37 ye, o ye fɛnɲɛnama misɛnninw dɔnni ye (Toll-like receptors (TLRs) 2 ani -4, CD32, CD206, Dectin-1, CD35, LOX-1, CD41, CD62P, ani αIIbβ3 integrin) ani mɔlikulu minnu bɛ tali kɛ antigènes jirali la T lanposotiw la (CD80, CD86, ani HLA-ABC) bɛ caya, ani banakisɛfagalanw bɛ bɔ: banakisɛfagalan/poroteyini min bɛ se ka don mɔgɔ kɔnɔ (BPI), azurocidin, hadamaden ka neutrophil peptide (HNP)-1, ani myeloperoxidase. U bɛ azurosidini fana baara ani ka Escherichia coli, Staphylococcus aureus ani Candida albicans sirili bonya. Ka fara o kan, joli segin furakisɛ minnu furakɛra ni LL-37 ye olu sanfɛla bɛ se ka Escherichia coli falenni bali, walima joli segin furakisɛw bɛ se ka baara kɛ n’u ka LL-37 ye walasa ka banakisɛw ka bonya bali^, .

A kɛcogo furaw dicogo la

Kɔlɔsiliw y’a jira ko banakisɛfagalan pepitiriw (AMP) ye biyomolekuli suguya kura ye min ka banakisɛfagalanw ka bon wa u ye mɔgɔw hakili sama ka da banakisɛfagalanw tali teliya ^kan , LL37 ye banakisɛfagalan pepitiri kelenpe ye min bɛ sɔrɔ katelisidini fɛ, min bɛ sɔrɔ hadamadenw na. Ni ɲinini kunbabaw kɛra, LL37 ye ɲɛnamaya baara suguya caman jira, i n’a fɔ funu jaabi labɛnni, farikolo tangalanw ka kemikorosi, joginda kɛnɛyali sabatili, ani koloci, o minnu ye dusu don kɛnɛyaso baara suguya caman na. Nka, LL37 bamanankan bamanankan na, dan ye a ka teli ka poroteyizi tiɲɛni na, a bɛ se ka kɛ baga ye, a tɛ se ka sɔrɔ biyo la, a ɲɔgɔnnaw.

Kuncɛli la, i n’a fɔ pepitiri biyoaktivi min bɛ baara caman kɛ, LL-37 ye sebaayaba jira kɛnɛyaso baarakɛcogo la. Kolow labɛnni sabatili siratigɛ la, fɛɛrɛ caman ka jɛɲɔgɔnya nɔfɛkow fɛ i n’a fɔ kolotugudaw danfara ni baara sabatili, banakisɛfagalanw, farikolo tangacogo, ani joli-sira-funu sabatili, o ye jigiya kura lase kolo joginw dilancogo ma. Ni banakisɛfagalanw bɛ se ka banakisɛw kɛlɛ, ni a bɛ selilɛri tiɲɛ k’a ɲɛsin a yɛrɛ ma, ka biyofilimuw dilanni bali, ani ka jɛɲɔgɔnya kɛ ni banakisɛfagalanw ye, a jirala ko a bɛna kɛ marifa barikama ye walasa ka banakisɛw gɛlɛya ɲɛnabɔ. Fura dicogo la, ni banakisɛfagalan pepitiriw misaliw dilanni n’u kɛcogo ɲuman ye, ka fura dicogo caman dilan, ani ka furaw tali faralen ɲɔgɔn kan, a furakɛli nɔ bɛ se ka bonya ka taa a fɛ. Kuma surun na, LL-37 bɛ se ka kɛ kɛnɛyaso baarakɛcogo caman na, i n’a fɔ kolo labɛnni sabatili, banakisɛfagalanw tali, joli segin furakisɛw ka banakisɛfagalanw baara baara, ani a kɛli furaw dicogo la.

A sɛbɛnbaga ko la

Fɛn minnu kofɔlen dòn san fɛ, olu bɛɛ ɲinini Kɛra, k’u Labɛn ani k’u Lajɛ Cocer Peptides fɛ.

Dɔnniya gafe sɛbɛnbaga

Francisco J. Sanchez-Pena ye ɲininikɛla ye Universidad Autonoma Benito Juarez de Oaxaca (Oaxaca ka Iniwɛrisite yɛrɛmahɔrɔnyalen Benito Juarez). Nin inivɛrisite in sigira senkan san 1827, a ye foroba kalanso nafama ye Oaxaca, Mɛkisiki, a bɛ kalan bolodalen caman di baarabolow la i n’a fɔ sigida dɔnniyaw, ɛntɛrinɛti, hadamadenya dɔnniyaw, ani sigidako dɔnniyaw.

Francisco J. Sanchez-Pena ka ɲininiw sinsinnen bɛ Biochimie & Moleculaire Biologie, Microbiologie ani Chimie kan. O kalan ninnu bɛ tali kɛ fɛnɲɛnamaw kɔnɔ kemikɛli taabolo kalanni na, molekiyɔmuw jɔcogo n’u baarakɛcogo, fɛnɲɛnamaw cogoyaw n’u ni sigida cɛsiraw, ka fara kemikɛlifɛnw danfara, u cogoya ani u jiginni sariyaw kan. Sɛgɛsɛgɛli minnu kɛra o yɔrɔw la, olu nafa ka bon furakɛli, sɛnɛ, sigida dɔnniya ani fɛn wɛrɛw la. Francisco J. Sanchez-Pena tɔgɔ sɛbɛnnen bɛ citation (citation) kɔnɔ [5].

▎ Sɛbɛn minnu bɛ tali kɛ o la

[1] Neville F, Cahuzac M, Konovalov O, ani a ɲɔgɔnnaw. Lipidi kunkolodimi danfara ni banakisɛfagalan pepitiri LL-37 ye: Hakilila min bɛ a kɛcogo kan[J]. Biophysique Journal, 2006,90(4):1275-1287.DOI:10.1529/biyofisiki.105.067595.

[2] Neshani A, Zare H, Eidgahi MRA, ani a ɲɔgɔnnaw. LL-37 : Banakisɛfagalanw ka ɲɛfɔli seginnkanni hadamadenw ka banakisɛfagalanw kan minnu bɛ se ka banakisɛfagalanw sɔrɔ ani minnu bɛ se ka banakisɛfagalanw kɛlɛ[J]. Jɛnɛya ka kunnafoniw, 2019,17:100519.DOI:10.1016/j.genrep.2019.100519.

[3] Li L, Peng Y, Yuan Q, ani mɔgɔ wɛrɛw. Cathelicidin LL37 bɛ kolotugudaw danfara sabati in vitro ani kolo lasegincogo in vivo[J]. Dancɛw Bioengineering ni Biotechnologie la, 2021,9.DOI:10.3389/fbioe.2021.638494.

[4] Kang J, Dietz MJ, Li B. Banakisɛfagalan pepitiri LL-37 ye banakisɛfagalan ye Staphylococcus aureus biyofilimuw kama[J]. Plos One, 2019,14(6).DOI:10.1371/sɛbɛnni.pone.0216676.

[5] Sanchez-Pena F. J., Romero-Tlalolini MDLA, Torɛsi-Aguilar H, ani mɔgɔ wɛrɛw. LL-37 bɛ banakisɛfagalanw baara daminɛ hadamadenw ka joli segin furakisɛw la[J]. Duniya kɔnɔ, 2023,24(3).DOI:10.3390/ijms24032816.

[6] Lin X, Wang R, Mai S. Ɲɛtaa sɔrɔli jiginni siratigɛ la LL37 furakɛli furakɛli la[J]. Fura dicogo dɔnniya ni seko ni dɔnko gafe, 2020,60.DOI:10.1016/j.jddst.2020.102016.

BƐƐRƐ KUNNAFONIW NI FƐRƐW KUNNAFONIW BƐƐ MIN BƐ NI WEBSITE KAN NA, U BƐ KUNNAFONIW FƆLƆ DƆRƆN NI KALANSENW YE.  

Fɛn minnu bɛ di nin siti in kan, olu dabɔra ɲininiw dɔrɔn kama in vitro. Sɛgɛsɛgɛli in vitro (latinkan na: *glasi kɔnɔ*, o kɔrɔ ye gilasi kɔnɔ) bɛ kɛ hadamaden farikolo kɔkan. Nin fura ninnu tɛ furaw ye, Ameriki ka dumuniko ni furako ɲɛmɔgɔso (FDA) ma sɔn u ma, wa u man kan ka kɛ ka furakɛli bana, bana walima bana si bali, k’a furakɛ, walima k’a furakɛ. A dagalen don kosɛbɛ sariya fɛ ka nin fɛn ninnu don hadamaden walima bagan farikolo la cogo o cogo.