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1kits (10Vials) .
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▎ LL-37 ƒe Dɔwɔɖoɖo
Dzɔtsoƒe: PubChem |
Nusiwo woɖo ɖe wo nɔewo yome: LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES Molekyulãwo ƒe Mɔfiame: C 205H 340N 60O53 Molekyulã ƒe Kpekpeme: 4493 g/mol CAS ƒe xexlẽdzesi: 154947-66-7 PubChem ƒe CID: 16198951 Nya siwo sɔ kple wo nɔewo: Cathelicidin;ropocamptide |
▎ LL-37 Numekuku
Nukae nye numekuku si wowɔ le LL-37 ŋu?
Swedentɔ dzɔdzɔmeŋutinunyala Boman HG ye ke ɖe LL-37 ŋu zi gbãtɔ be enye cationic peptide suewo ƒe hatsotso aɖe le seda nudzodzoe Samia cynthia ricini ƒe pupae me le ƒe 1980. LL-37 nye amegbetɔ ƒe cathelicidin antibacterial peptide ƒe C-terminal peptide (CAMP, hCAP18), si ate ŋu ana woate ŋu atsi dɔlékui suewo nu ɖe edzi amedzidzedze eye wòwɔa ŋutilãmedɔ veviwo le chemotaxis me, doa abi ƒe nutsotso ɖe ŋgɔ, kple ʋukawo ƒe dzɔdzɔ (Chen X, 2018). Peptide siwo wua dɔlékuiwo le lãwo, numiemiewo, kple nugbagbevi suesuesue ʋɛ aɖewo me geɖe, eye wonye akɔtalãwo ƒe dɔlélenutsiŋutete si wodzina ƒe akpa vevi aɖe. Abe protein si ɣlaa nu ene la, LL-37 le amegbetɔ ƒe ŋutilã ƒe ŋutinu kple lãkusi geɖe me. Lãmenugbagbevi vovovowo, siwo dometɔ aɖewoe nye lãmenugbagbevi siwo le lãkusi me, keratinocytes, mast cells, neutrophils, macrophages, kple monocytes, ate ŋu aɖee ɖa. Dɔlékuiwutike sia ƒomevi ƒe akpa gãtɔ me 'amino acid residues' 37 - 39 le eye cysteine 0 le eme. Le gɔmeɖoanyi sesẽ si le dɔlékuiwutike ƒe peptide ƒe N-nuwuƒe nɔƒe ta la, ateŋu awɔ 'amphiphilic helical structure' si li ke. Dɔlékuiwutike peptide LL-37 hã ƒe 'amphiphilic α-helical structure' le esi. Le dɔ si wòwɔna be wòawu dɔlékui siwo hea dɔlékuiwo ta la, wotsɔ ŋkɔ nɛ be peptide si tsia dɔlékuiwo nu. '37' si le ŋkɔ LL-37 me ateŋu ado ƒome kple eƒe amino acid ƒe nugbagbevi susɔewo ƒe xexlẽme. Le ɣeyiɣi ma ke me la, wogayɔnɛ be cathelicidin kple ropocamptide.
Nukae nye LL-37 ƒe dɔwɔwɔ ɖe dɔlékui siwo te ŋu nɔa te ɖe dɔlékuiwutikewo nu ŋu?
Dɔlékui ƒe lãmenugbagbeviwo ƒe lãkusi ƒe tɔtɔ:
LL-37 ate ŋu age ɖe dɔlékui ƒe lãmenugbagbeviwo ƒe lãkusi me, vevietɔ ate ŋu agblẽ nu le lãmenugbagbevi ƒe lãkusi si me phosphatidylglycerol (DPPG) le ŋu. Agblẽ dɔlékui ƒe lãmenugbagbeviwo ƒe lãkusi ƒe wɔwɔme me, si ana wòawɔ dɔlékuiwutike [1] . Le kpɔɖeŋu me, numekukuwo ɖee fia be LL-37 ate ŋu age ɖe dɔlékui siwo nye Gram-positive kple Gram-negative siaa ƒe lãmenugbagbeviwo ƒe lãkusiwo me, si wɔnɛ be lãmenugbagbeviwo ƒe lãkusi ƒe gege ɖe lãme na ame dzina ɖe edzi, lãmenugbagbeviwo me nuwo ƒe dodo, eye mlɔeba dɔlékuiwo kuna.
Dɔlékuiwutikewo ƒe dɔwɔwɔ le mɔ gbadza nu:
Dɔlékuiwutike ƒe dɔwɔwɔ le LL-37 ŋu ɖe dɔlékui vovovo siwo te ŋu nɔa te ɖe dɔlékuiwutikewo nu ŋu. Ate ŋu awɔ dɔ ɖe dɔlékui siwo ŋu Gram-positive le (abe Staphylococcus aureus, Streptococcus, Enterococcus, kple bubuawo), dɔlékui siwo me Gram-negative le (abe Pseudomonas aeruginosa, Escherichia coli, Salmonella, kple bubuawo ene), kple dɔlékui ƒe dɔlékui bubuwo (abe Mycoplasma, Ureaplasma, Mycobacterium, kple bubuawo ene) ŋu [2] . Dɔlékuiwutike ƒe dɔwɔwɔ sia si keke ta na be asixɔxɔ si woate ŋu azã le LL-37 ŋu le avuwɔwɔ kple dɔlékui ƒomevi vovovo siwo te ŋu nɔa te ɖe dɔlékuiwutikewo nu me.
Biofilm si wowɔ la tsɔtsrɔ̃:
Dɔlékui ƒe biofilm nye susu vevi siwo ta dɔlékui siwo hea dɔlékuiwo te ŋu nɔa te ɖe atikewo nu la dometɔ ɖeka. Peptide si tsia dɔlékuiwo nu si nye LL-37 ate ŋu agblẽ biofilm si wowɔ la dome, si ana dɔlékuiwo ƒe atikewo ƒe dɔlélenutsiŋutete dzi naɖe akpɔtɔ. Le kpɔɖeŋu me. Gake LL-37 ate ŋu awɔ akpa nyui aɖe le dɔlékuiwutike kple dɔlékuiwutikewo nu to mɔxexe ɖe biofilm ƒe wɔwɔ nu kple biofilm si wowɔ la tsɔtsrɔ̃ me.
Dɔlékuiwutikewo ƒe dɔlékuiwutikewo ƒe dɔwɔwɔ dzi ɖeɖe kpɔtɔ:
Numekukuwo ɖee fia be LL-37 wɔa dɔ aduadu kple dɔlékuiwutike aɖewo. Le kpɔɖeŋu me, ne wozãe kpe ɖe amoxicillin clavulanic acid (AMC) ŋu la, LL-37 ate ŋu ana AMC ƒe dɔlékuiwutikewo nadzi ɖe edzi vevie.
Dzɔtsoƒe:PubMed [6] .
Nukae nye LL-37 ƒe dɔbiagbalẽviwo?
Ƒuwo ƒe gbugbɔgadzɔ dodo ɖe ŋgɔ:
Numekuku aɖewo ɖee fia be dɔlékuiwutike si nye peptide LL-37 kpɔa ŋusẽ nyui ɖe ƒuwo ƒe gbugbɔgadzɔ dzi. Numekuku aɖewo ɖee fia be wotsɔ LL-37 ƒe agbɔsɔsɔ vovovowo de amegbetɔ ƒe ami me lãmenugbagbeviwo (hADSCs) me, eye wokpɔe be LL-37 ƒe agbɔsɔsɔ kpɔ ŋusẽ ɖe hADSCs ƒe ƒumeŋuɖui ƒe ŋutete dzi, eye wòɖo eƒe kɔkɔƒe le 4μg/ml. Tsɔ kpe ɖe eŋu la, PSeD/hADSCs/LL-37 ƒokpli ƒe ʋuʋudedi ɖe ƒumeŋuɖui ƒe nɔnɔme siwo de ŋgɔ wu fia wu PSeD/hADSCs, PSeD, kple ƒuƒoƒo si me wokpɔa wo gbɔ le ƒe ʋuƒo siwo le sisiblisi ƒe akɔta ƒe nɔnɔme madeamedzi ƒe kpɔɖeŋua me, si ɖee fia be ŋutete gã aɖe le eŋu le ƒu ƒe gbugbɔgadzɔ le kɔdzi me.
Dɔlékuiwutikewo ƒe ŋusẽkpɔɖeamedzi:
Dɔlékui geɖe siwo hea dɔlékuiwo ƒe mɔxexe ɖe enu:
Numekuku aɖewo zã micro-double dilution mɔnu tsɔ de dzesi dɔlékuiwutike peptide LL-37 ƒe mɔxexeɖedɔléle nu ƒe agbɔsɔsɔ suetɔ kekeake (MIC) ɖe Escherichia coli, Salmonella, kple Staphylococcus aureus ŋu. Nusiwo do tso eme ɖee fia be LL-37 ƒe mɔxexeɖedɔlékui etɔ̃ siawo ƒe mɔxexeɖedɔléle nu ƒe agbɔsɔsɔme to vovo, eye mɔxexeɖedɔléle nu ƒe agbɔsɔsɔ suetɔ kekeake nye 3.12, 1.56, kple 0.78μg/mL. Dzoxɔxɔ ƒe liƒo dodokpɔa ɖee fia be dɔlékuiwutike si wogbugbɔ ƒo ƒu la gakpɔtɔ wɔa dɔ nyuie le dzoxɔxɔ gã me. Acid-base stability dodokpɔ me tsonu ɖee fia be LL-37 ƒe dɔwɔwɔ aɖe le pH ƒe domedome 2.0 va ɖo 12.0, eye dɔwɔwɔ nyuitɔ kekeake le pH 5.0 va ɖo 6.0 me, eye -20°C nye nɔnɔme nyuitɔ kekeake si me woate ŋu adzrae ɖo ɣeyiɣi didi [3]..
Dɔlékui siwo te ŋu nɔa te ɖe dɔlékuiwutikewo nu dzi:
Ame aɖewo srɔ̃ nu tso dɔlékuiwutike peptide LL-37 kple klosalo nanoparticles (AgNPs) ƒe dɔwɔwɔ ɖe Staphylococcus aureus (S. aureus), si nye nugbagbevi sue aɖe si wokpɔna zi geɖe le dɔlékui siwo do ƒome kple biofilm me ŋu. Nusiwo do tso eme ɖee fia be LL-37 ye nye dɔlékuiwutike si wɔa dɔ wu, eye eƒe ƒuƒoƒo ƒe xexlẽme dzi ɖe kpɔtɔ wu logarithm 4. To vovo na ema la, klosalo ƒe nanoparticles kple dɔlékuiwutike siwo wozãna ɖaa ƒe ŋusẽkpɔɖeamedzi mede ŋgɔ o, eye colony ƒe xexlẽme dzi ɖe kpɔtɔ mede logarithm 1 o. Dɔlékuiwutikewo zazã ɖekae kple rifampicin na AgNPs kple gentamicin ƒe logarithmic dzi ɖeɖe kpɔtɔ ɖe edzi ŋutɔ, gake egaɖiɖi ŋutɔ wu LL-37 si wozã ɖeɖeko tɔ [4]..
Dɔwɔwɔ le aklãdɔléle me:
Numekukuwo ɖee fia be Pseudomonas aeruginosa (PA) va zu kuxi kpata aɖe na aklãdɔléle kple aklã me abixɔxɔ. LL37 peptide nye dɔlékuiwutike nyui aɖe si tsia tre ɖe PA ƒomeviwo ŋu, gake eƒe zazã mede enu o le eƒe ɖeɖeɖa kabakaba le agbe me, nugbagbewo ƒe dedienɔnɔ ŋuti nyawo, kple nugbagbewo ƒe agbɔsɔsɔ si mede o ta. Eyata woto nanodrug delivery system si wotu ɖe albumin si sea nu dzi ɖeɖe kpɔtɔ dzi vɛ be wòana LL37 ƒe dɔwɔwɔ ɖe PA ŋu nanyo ɖe edzi le vivo to molecule dome disulfide kadodowo wɔwɔ me. Woate ŋu atsɔ cationic LL37 abla nyuie to elektrikŋusẽ ƒe kadodo me be wòawɔ dɔlékuiwutike ƒe ŋusẽkpɔɖeamedzi si nyo wu. LL37 peptide la ɖee fia be woɖe asi le eŋu ɣeyiɣi didi wu gaƒoƒo 48 tso LL37 peptide nanoparticles (LL37 PNP) me, eye wode dzesi dɔlékuiwutike ƒe ŋusẽkpɔɖeamedzi si keke ta esi ɣeyiɣi si wotsɔ ƒoe la dzi ɖe edzi. Le PA ƒe aklãdɔléle sesẽ ƒe kpɔɖeŋu si wowɔ le sisiblisi me la, LL37 PNP ɖe TNF-α kple IL-1β ƒe ɖeɖefia dzi kpɔtɔ ŋutɔ eye wòɖe abi si woxɔna le lãkusi me dzi kpɔtɔ. Eɖee fia be LL37 PNP ate ŋu ana PA ƒe aklãdɔléle kple dɔdzẽ ƒe ŋuɖoɖo si kplɔe ɖo nanyo ɖe edzi nyuie wu LL37 peptide si le ablɔɖe me [3]..
Ʋumenugbagbevi dzĩwo ƒe dɔlékuiwutikewo ƒe dɔwɔwɔ:
Numekukuwo ɖee fia be dɔlékuiwutike peptide LL-37 ate ŋu ana amegbetɔ ƒe ʋumenugbagbevi dzĩwo nawɔ dɔ ɖe dɔlékuiwutikewo dzi. Ne wotsɔ LL-37 da dɔ na ʋumenugbagbevi dzĩwo vɔ la, nugbagbevi suesue siwo dea dzesi ʋumenugbagbevi dzĩwo ƒe gotagome ɖeɖefia (Toll-like receptors (TLRs) 2 kple -4, CD32, CD206, Dectin-1, CD35, LOX-1, CD41, CD62P, kple αIIbβ3 integrin) kple molecule siwo do ƒome kple dɔlékuiwutikewo tsɔtsɔ yi T lymphocytes gbɔ (CD80, CD86, kple HLA-ABC) dzina ɖe edzi, eye woɖea dɔlékuiwutikewo ƒe moleculewo dona: dɔlékuiwutike/protein si dzia dɔlékuiwo ƒe gege ɖe lãme na ame (BPI), azurocidin, amegbetɔ ƒe neutrophil peptide (HNP)-1, kple myeloperoxidase. Woɖea azurocidin hã gɔme eye wodoa kadodo ɖe Escherichia coli, Staphylococcus aureus, kple Candida albicans ŋu ɖe edzi. Tsɔ kpe ɖe eŋu la, ʋumenugbagbevi dzĩ siwo wotsɔ LL-37 da dɔe ƒe tsi si le tsia dzi ate ŋu axe mɔ na Escherichia coli ƒe tsitsi, alo ʋumenugbagbevi dzĩwo ate ŋu azã woƒe LL-37 atsɔ axe mɔ na nugbagbevi suewo ƒe tsitsi [5]..
Dɔwɔwɔ le atikewo nana ƒe ɖoɖowo me
Numekukuwo gblɔ be dɔlékuiwutikewo ƒe peptidewo (AMPs) nye biomolecules ƒe hatsotso yeye aɖe si si dɔlékuiwutike ƒe ŋutete siwo keke ta le eye wohe amewo ƒe susu le dɔlékuiwutikewo ƒe dɔlélenutsiŋutete ƒe dzidziɖedzi kabakaba ta [6] . LL37 koe nye dɔlékuiwutike si wokpɔna tso cathelicidin me si wokpɔna le amegbetɔwo me. To numekuku deto me la, LL37 ɖe nugbagbeviwo ƒe dɔwɔwɔ vovovowo fia, siwo dometɔ aɖewoe nye dɔdzẽ ƒe dɔwɔwɔ dzi kpɔkpɔ, dɔlélenutsiŋutete ƒe lãmenugbagbeviwo ƒe chemotaxis, abi ƒe dɔyɔyɔ dodo ɖe ŋgɔ, kple ƒu ƒe dzɔdzɔ, siwo de dɔdamɔnu vovovowo zazã ƒe dzi ƒo. Ke hã, LL37 ƒe dɔdamɔnu gɔmeɖeɖe seɖoƒe li na le eƒe seselelãme ɖe protease ƒe gbegblẽ ŋu, aɖi si ate ŋu anɔ eme, nugbagbewo ƒe anyimanɔmanɔ nyuie, kple bubuawo.Woto mɔnu vovovowo vɛ, siwo dometɔ aɖewoe nye ga nanoparticles, polymer materials, kple lipid-based systems, be woakpɔ atikewɔwɔ ƒe dɔwɔwɔwo gbɔ.
Le nyataƒoƒo me la, abe peptide si wɔa dɔ geɖe le nugbagbewo me ene la, LL-37 ɖe ŋutete gã aɖe fia le dɔdamɔnu zazã me. Le ƒu ƒe gbugbɔgadzɔ dodo ɖe ŋgɔ gome la, to mɔnu vovovowo abe ƒumeŋuɖui ƒe vovototodedeameme kple dɔwɔwɔ dodo ɖe ŋgɔ, dɔlékuiwutikewo ƒe ŋusẽkpɔɖeamedzi, dɔlélenutsiŋutete ƒe tɔtrɔ, kple ʋukawo ƒe dzɔdzɔ dodo ɖe ŋgɔ ƒe dɔwɔwɔ aduadu me la, ehe mɔkpɔkpɔ yeye vɛ na ƒu ƒe abi ɖɔɖɔɖo. Ne wole nu wɔm kple dɔlékui siwo te ŋu nɔa te ɖe dɔlékuiwutikewo nu, to lãmenugbagbeviwo ƒe lãkusi tsɔtsrɔ̃ tẽ, mɔxexe ɖe biofilm ƒe wɔwɔ nu, kple dɔwɔwɔ aduadu kple dɔlékuiwutikewo me la, wokpɔa mɔ be ava zu aʋawɔnu sẽŋu aɖe si woatsɔ akpɔ dɔlékui siwo te ŋu nɔa te ɖe atikewo nu ƒe kuxia gbɔ. Le atikewo nana ƒe ɖoɖowo me la, to dɔlékuiwutikewo ƒe peptide ƒe nɔnɔmetatawo wɔwɔ kple wo ŋudɔwɔwɔ nyuie wu, atikewo nana ƒe ɖoɖo geɖewo wɔwɔ, kple atikewo zazã ɖekae me dzodzro me la, woate ŋu adzi eƒe dɔdamɔnu ƒe ŋusẽkpɔɖeamedzi ɖe edzi. Kpuie ko la, ŋutete le LL-37 ŋu le dɔdamɔnu ƒe akpa vovovowo zazã me, siwo dometɔ aɖewoe nye ƒu ƒe gbugbɔgadzɔ dodo ɖe ŋgɔ, dɔlékuiwutikewo ƒe ŋusẽkpɔɖeamedzi, ʋumenugbagbevi dzĩwo ƒe dɔlékuiwutikewo ƒe dɔwɔwɔ, kple eŋudɔwɔwɔ le atikewo nana ƒe ɖoɖowo me.
Le Agbalẽŋlɔla La Ŋu
Nusiwo míeyɔ ɖe etame la katã nye esiwo ŋu Cocer Peptides wɔ numekuku le, trɔ asi le wo ŋu eye woƒo wo nu ƒu.
Dzɔdzɔmeŋutinunya ƒe Agbalẽŋlɔla
Francisco J. Sanchez-Pena nye numekula le Universidad Autonoma Benito Juarez de Oaxaca (Benito Juarez Yunivɛsiti si le eɖokui si le Oaxaca). Woɖo yunivɛsiti sia le ƒe 1827 me, eye wònye dukɔa ƒe dɔwɔƒe ɖedzesi aɖe le Oaxaca, Mexico, si naa sukudede ƒe ɖoɖo vovovowo le nusiwo nye dzɔdzɔmeŋutinunya, mɔ̃ɖaŋuŋutinunya, amegbetɔŋutinunya, kple hadomenunya me.
Francisco J. Sanchez-Pena ƒe numekukua ku ɖe Biochemistry & Molecular Biology, Microbiology, kple Chemistry ŋu. Nusɔsrɔ̃ siawo lɔ atikewo ƒe dɔwɔwɔ le nugbagbewo me, molecule ƒe wɔwɔme kple dɔwɔwɔ, nugbagbevi suewo ƒe nɔnɔmewo kple woƒe kadodo kple nutome, kpakple atikewo ƒe wɔwɔme, woƒe nɔnɔme, kple woƒe tɔtrɔ ƒe sewo sɔsrɔ̃ ɖe eme. Numekuku siwo wowɔ le go siawo me wɔa dɔ vevi aɖe le atikewɔwɔ, agbledede, nutomeŋutinunya, kple bubuwo me. Woŋlɔ Francisco J. Sanchez-Pena ŋkɔ ɖe nyayɔyɔ ƒe nyatakaka me [5].
