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1 kits (10 Vials)
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▎ Tsarin LL-37
Source: PubChem |
Jeri: LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES Tsarin kwayoyin halitta: C 205H 340N 60O53 Nauyin Kwayoyin: 4493 g/mol Lambar CAS: 154947-66-7 PubChem CID: 16198951 Synonyms: Cathelicidin; ropocamptide |
▎ LL-37 Bincike
Menene asalin bincike na LL-37?
LL-37 an fara gano shi a matsayin nau'in cationic kananan peptide abubuwa a cikin pupae na silkworm Samia cynthia ricini ta masanin kimiyyar Sweden Boman HG a cikin 1980. LL-37 shine peptide C-terminal (CAMP, hCAP18) na ɗan adam cathelicidin peptide peptide na ɗan adam yana iya haɓaka juriya na ƙwayoyin cuta da ƙwayoyin cuta masu mahimmanci a cikin ayyukan microbitic. chemotaxis, inganta ƙulli rauni, da angiogenesis (Chen X, 2018). Ana samun peptides na ƙwayoyin cuta a ko'ina a cikin dabbobi, shuke-shuke, da ƙananan ƙwayoyin cuta, kuma suna da mahimmanci na rigakafi na asali na vertebrates. A matsayin furotin na sirri, LL-37 yana yadu a cikin gabobin gabobin da kyallen jikin mutum. Kwayoyin daban-daban, ciki har da ƙwayoyin epithelial, keratinocytes, ƙwayoyin mast, neutrophils, macrophages, da monocytes, na iya ɓoye shi. Yawancin irin wannan maganin rigakafi ya ƙunshi 37 - 39 ' ragowar amino acid' kuma yana da 0 cysteine. Saboda ƙaƙƙarfan asali a matsayin N-terminal na peptide na ƙwayoyin cuta, zai iya samar da barga 'tsarin helical amphiphilic'. Peptide na antibacterial LL-37 kuma yana da tsarin α-helical amphiphilic. Saboda aikinsa na kashe ƙwayoyin cuta, ana kiransa peptide na ƙwayoyin cuta. The '37' a cikin sunan LL-37 ƙila yana da alaƙa da adadin ragowar amino acid ɗin sa. A lokaci guda, ana kuma san shi da cathelicidin da ropocamptide.
Menene tsarin aikin LL-37 akan ƙwayoyin cuta masu jurewa?
Rushe membrane cell cell:
LL-37 na iya sakawa a cikin ƙwayar ƙwayar ƙwayar ƙwayar cuta, musamman ma yana da tasiri mai lalacewa a kan membrane na tantanin halitta wanda ke dauke da phosphatidylglycerol (DPPG). Zai rushe tsarin membrane na kwayan cuta, don haka yana haifar da sakamako na ƙwayoyin cuta [1] . Misali, bincike ya gano cewa LL-37 na iya sakawa a cikin membranes tantanin halitta na kwayoyin cutar Gram-positive da Gram-negative, wanda ke haifar da karuwa a cikin iyawar kwayar halitta, zubar da abin da ke cikin tantanin halitta, kuma a ƙarshe mutuwar kwayoyin cutar.
Babban aikin antibacterial:
LL-37 yana da aikin antibacterial akan nau'ikan ƙwayoyin cuta masu jurewa iri-iri. Yana iya aiki da kwayoyin cutar Gram-tabbatacce (irin su Staphylococcus aureus, Streptococcus, Enterococcus, da dai sauransu), kwayoyin cutar Gram (irin su Pseudomonas aeruginosa, Escherichia coli, Salmonella, da dai sauransu), da sauran ƙwayoyin cuta (irin su Mycoplasma, Ureaumplasma, da dai sauransu ) . Wannan aikin kashe kwayoyin cuta mai fadi yana sa LL-37 ya sami yuwuwar ƙimar aikace-aikacen yaƙi da nau'ikan ƙwayoyin cuta masu jurewa iri-iri.
Lalacewar da aka kafa na biofilm:
Bacterial biofilm yana daya daga cikin mahimman dalilai na juriya na miyagun ƙwayoyi na ƙwayoyin cuta. peptide na antibacterial LL-37 na iya halakar da kafawar biofilm, don haka rage juriya na kwayoyin cuta. Alal misali, a cikin kamuwa da cuta na haɗin gwiwa (PJI) bayan maye gurbin haɗin gwiwa na wucin gadi, juriya na miyagun ƙwayoyi na ƙwayoyin cuta da kwayoyin halitta suka haifar da kwayoyin halitta suna sa maganin ya yi wahala. Duk da haka, LL-37 na iya taka rawa mai tasiri na antibacterial da bacteriostatic ta hanyar hana samuwar biofilm da lalata da aka kafa.
Inganta aikin antibacterial na maganin rigakafi:
Nazarin ya nuna cewa LL-37 yana da tasirin daidaitawa tare da wasu maganin rigakafi. Misali, idan aka yi amfani da shi tare da amoxicillin clavulanic acid (AMC), LL-37 na iya haɓaka aikin ƙwayoyin cuta na AMC da ƙarfi.
Source:PubMed [6]
Menene aikace-aikacen LL-37?
Inganta farfadowar kashi:
Wasu nazarin sun nuna cewa peptide antibacterial LL-37 yana da tasiri mai kyau akan farfadowa na kashi. Wasu bincike sun nuna cewa ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin cuta (hADSCs) sun haɓaka tare da nau'i daban-daban na LL-37, kuma an gano cewa ƙaddamar da LL-37 yana da tasiri a kan iyawar osteogenic na hADSCs, ya kai kololuwa a 4μg / ml. Bugu da ƙari, haɗin haɗin PSeD / hADSCs / LL-37 ya nuna mafi kyawun kaddarorin osteogenic fiye da PSeD / hADSCs, PSeD, da ƙungiyoyi masu kula da su a cikin ƙirar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta.
Tasirin Antibacterial:
Hana ƙwayoyin ƙwayoyin cuta masu yawa:
Wasu bincike sun yi amfani da hanyar dilution micro-biyu don ƙayyade mafi ƙarancin inhibitory taro (MIC) na peptide antibacterial LL-37 a kan Escherichia coli, Salmonella, da Staphylococcus aureus. Sakamakon ya nuna cewa LL-37 yana da nau'i daban-daban na tasirin hanawa akan waɗannan ƙwayoyin cuta guda uku, tare da ƙananan ƙananan ƙwayoyin cuta shine 3.12, 1.56, da 0.78μg / ml, bi da bi. Gwajin kwanciyar hankali na thermal ya nuna cewa recombinant antibacterial peptide har yanzu yana da kyakkyawan aiki a yanayin zafi. Sakamakon gwajin kwanciyar hankali na acid-base ya nuna cewa LL-37 yana da wasu ayyuka a kewayon pH na 2.0 zuwa 12.0, tare da mafi kyawun aiki a pH na 5.0 zuwa 6.0, da -20°C shine mafi kyawun yanayin ajiya na dogon lokaci [3].
Tasiri kan ƙwayoyin cuta masu jure wa ƙwayoyin cuta:
Wasu mutane sun yi nazarin tasirin ƙwayoyin cuta na peptide antibacterial LL-37 da azurfa nanoparticles (AgNPs) a kan Staphylococcus aureus (S. aureus), kwayoyin halitta da aka fi samu a cikin cututtuka masu alaka da biofilm. Sakamakon ya nuna cewa LL-37 shine mafi kyawun maganin kashe kwayoyin cuta, tare da raguwa a cikin ƙididdiga na mallaka fiye da 4 logarithms. Sabanin haka, illar nanoparticles na azurfa da maganin rigakafi na al'ada sun fi talauci, tare da raguwar ƙidayar mazauna ƙasa da logarithm 1. Maganin haɗin gwiwar ƙwayoyin cuta tare da rifampicin yana haɓaka raguwar logarithmic na AgNPs da gentamicin, amma har yanzu yana da ƙasa da na LL-37 da aka yi amfani da shi kaɗai [4].
Aikace-aikace a cikin ciwon huhu:
Nazarin ya nuna cewa Pseudomonas aeruginosa (PA) ya zama ƙalubale na gaggawa don kamuwa da cutar huhu da ciwon huhu. LL37 peptide ingantaccen wakili ne na ƙwayoyin cuta akan nau'ikan PA, amma aikace-aikacen sa yana iyakance saboda saurin sharewar sa a cikin vivo, batutuwan biosafety, da ƙarancin bioavailability. Sabili da haka, an haɓaka tsarin isar da nanorug na tushen albumin mai rahusa don haɓaka aikin LL37 akan PA a cikin vivo ta hanyar samar da haɗin gwiwar disulfide intermolecular. Cationic LL37 za a iya lullube shi da kyau ta hanyar hulɗar electrostatic don aiwatar da ingantaccen sakamako na ƙwayoyin cuta. LL37 peptide ya nuna ci gaba mai dorewa na sama da sa'o'i 48 daga LL37 peptide nanoparticles (LL37 PNP), kuma an lura da ƙarin tasirin ƙwayoyin cuta tare da haɓaka lokacin shiryawa. A cikin samfurin linzamin kwamfuta na m kamuwa da cutar huhu na PA, LL37 PNP ya rage yawan maganganun TNF-α da IL-1β da kuma rage raunin huhu. Yana nuna cewa LL37 PNP na iya inganta ingantaccen kamuwa da cutar huhu na PA da kuma amsawar kumburi mai zuwa fiye da peptide LL37 kyauta [3].
Kunna aikin antibacterial na platelet:
Nazarin ya nuna cewa peptide antibacterial LL-37 na iya kunna aikin ƙwayoyin cuta na platelet na ɗan adam. Bayan an yi amfani da platelets tare da LL-37, bayyanar bayyanar masu karɓa don gane ƙwayoyin cuta (Tll-like receptors (TLRs) 2 da -4, CD32, CD206, Dectin-1, CD35, LOX-1, CD41, CD62P, da αIIbβ3 integrin, CD62P, da αIIbβ3 integrin, da kuma kwayoyin cutar antigens, CD8 da ke da alaƙa da T8. HLA-ABC) yana ƙaruwa, kuma ƙwayoyin ƙwayoyin cuta suna ɓoye: bactericidal / permeability-creasing protein (BPI), azurocidin, peptide neutrophil na mutum (HNP) -1, da myeloperoxidase. Suna kuma fassara azurocidin kuma suna haɓaka ɗaure ga Escherichia coli, Staphylococcus aureus, da Candida albicans. Bugu da ƙari, mai girma na platelets da aka yi wa LL-37 zai iya hana ci gaban Escherichia coli, ko kuma platelets na iya amfani da LL-37 don hana ci gaban ƙananan ƙwayoyin cuta [5]..
Aikace-aikace a cikin tsarin isar da magunguna
Nazarin ya ambaci cewa peptides na ƙwayoyin cuta (AMPs) wani sabon nau'i ne na kwayoyin halitta tare da kaddarorin ƙwayoyin cuta masu yawa kuma sun jawo hankali saboda saurin haɓakar juriya na ƙwayoyin cuta [6] . LL37 shine kawai cathelicidin da aka samu peptide na ƙwayoyin cuta da aka samu a cikin mutane. Tare da bincike mai zurfi, LL37 ya nuna ayyuka daban-daban na ilimin halitta, ciki har da daidaitawa da amsawar kumburi, chemotaxis na ƙwayoyin rigakafi, inganta warkar da raunuka, da osteogenesis, wanda ya karfafa nau'o'in aikace-aikacen asibiti. Duk da haka, fassarar asibiti na LL37 yana da iyakancewa ta hanyar hankali ga lalatawar protease, yuwuwar guba, rashin lafiyar rayuwa, da dai sauransu. Tsarin bayarwa iri-iri, gami da nanoparticles na ƙarfe, kayan polymer, da tsarin tushen lipid, an gabatar da su don cimma aikace-aikacen warkewa.
A ƙarshe, a matsayin multifunctional bioactive peptide, LL-37 ya nuna babban yuwuwar a aikace-aikace na asibiti. Dangane da inganta haɓakar haɓakar kashi, ta hanyar tasirin haɗin gwiwa na hanyoyin da yawa kamar haɓaka bambance-bambancen osteoblast da aiki, tasirin antibacterial, immunomodulation, da haɓaka angiogenesis, ya kawo sabon bege don gyaran raunin kashi. Lokacin da ake yin maganin ƙwayoyin cuta masu jure wa ƙwayoyin cuta, ta hanyar lalata membrane tantanin halitta kai tsaye, hana samar da biofilms, da yin aiki da maganin rigakafi, ana sa ran ya zama makami mai ƙarfi don magance matsalar ƙwayoyin cuta masu jure wa magunguna. A cikin tsarin isar da magunguna, ta hanyar ƙira da haɓaka samfuran peptide na ƙwayoyin cuta, gina tsarin isar da magunguna da yawa, da kuma bincika haɗakar aikace-aikacen magunguna, ana iya ƙara haɓaka tasirin maganin sa na asibiti. A takaice, LL-37 yana da yuwuwar a cikin bangarori da yawa na aikace-aikacen asibiti, gami da haɓaka haɓakar kashi, tasirin ƙwayoyin cuta, kunna aikin ƙwayoyin cuta na platelets, da aikace-aikace a cikin tsarin isar da magunguna.
Game da Marubuci
Abubuwan da aka ambata a sama duk bincike ne, gyara su kuma Cocer Peptides ne suka haɗa su.
Mawallafin Jaridar Kimiyya
Francisco J. Sanchez-Pena mai bincike ne a Universidad Autonoma Benito Juarez de Oaxaca (Jami'ar Benito Juarez ta Oaxaca mai zaman kanta). An kafa shi a cikin 1827, wannan jami'a babbar cibiyar jama'a ce a Oaxaca, Mexico, tana ba da shirye-shiryen ilimi da yawa a fannoni kamar kimiyyar halitta, injiniyanci, ɗan adam, da ilimin zamantakewa.
Binciken Francisco J. Sanchez-Pena ya mayar da hankali kan Biochemistry & Molecular Biology, Microbiology, and Chemistry. Waɗannan fannonin sun haɗa da nazarin hanyoyin sinadarai a cikin kwayoyin halitta, sifofi da ayyuka na ƙwayoyin cuta, halayen ƙananan ƙwayoyin cuta da mu'amalarsu da muhalli, gami da ƙayyadaddun abubuwa, kaddarorin, da ƙa'idodin canza sinadarai. Bincike a cikin waɗannan yankuna yana da mahimman aikace-aikace a cikin magani, aikin gona, kimiyyar muhalli, da ƙari. Francisco J. Sanchez-Pena an jera shi a cikin ambaton ambaton [5].
