|
1 pahu (10 Vials)
| Ka nui: | |
|---|---|
▎ LL-37 Kūlana
Puna: PubChem |
Kaʻina: LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES Hui Molekala: C 205H 340N 60O53 Kaumaha Molekala: 4493 g/mol Helu CAS: 154947-66-7 PubChem CID: 16198951 Synonyms: Cathelicidin；ropocamptide |
▎ LL-37 Noiʻi
He aha ke kumu noiʻi o LL-37?
Ua ʻike mua ʻia ʻo LL-37 ma ke ʻano he papa o nā mea peptide liʻiliʻi cationic i loko o nā pupae o ka silkworm Samia cynthia ricini e ka mea ʻepekema Kuekene Boman HG ma 1980. ʻO LL-37 ka C-terminal peptide (CAMP, hCAP18) o ke kanaka cathelicidin antibacterial peptide i hiki ke hoʻonui i ka peptide antibacterial nui. i ka chemotaxis, hoʻolaha i ka pani ʻana i ka ʻeha, a me ka angiogenesis (Chen X, 2018). Aia nā peptides antibacterial i loko o nā holoholona, mea kanu, a me kahi liʻiliʻi o nā microorganisms, a he ʻāpana koʻikoʻi lākou o ka pale kino o nā vertebrates. Ma ke ʻano he protein huna, ʻike nui ʻia ʻo LL-37 i nā kino a me nā ʻiʻo o ke kino kanaka. Hiki i nā ʻāpana like ʻole, e like me nā epithelial cell, keratinocytes, mast cell, neutrophils, macrophages, a me nā monocytes, hiki ke hūnā iā ia. Loaʻa ka hapa nui o kēia ʻano lāʻau antibiotic he 37 - 39 'mau koena amino acid' a he 0 ka cysteine. Ma muli o ke kumu ikaika ma ke kūlana N-terminal o ka peptide antibacterial, hiki iā ia ke hana i kahi paʻa 'amphiphilic helical structure'. Loaʻa i ka peptide antibacterial LL-37 kahi 'amphiphilic α-helical structure'. Ma muli o kāna hana o ka pepehi ʻana i nā bacteria pathogenic, ua kapa ʻia ʻo ia he peptide antibacterial. Hiki ke pili ka '37' ma ka inoa LL-37 i ka heluna o kona mau koena amino acid. I ka manawa like, ua kapa ʻia ʻo cathelicidin a me ropocamptide.
He aha ke ʻano o ka hana a LL-37 e kūʻē i ka maʻi maʻi kūʻē antibiotic?
Hoʻopilikia i ka membrane cell bacteria:
Hiki i ka LL-37 ke hoʻokomo i loko o ka membrane cell bacteria, ʻoi aku ka loaʻa ʻana o ka hopena luku i ka membrane cell i loaʻa ka phosphatidylglycerol (DPPG). E hoʻopau ia i ke ʻano o ka membrane cell bacteria, pēlā e hoʻokō ai i kahi hopena bactericidal [1] . No ka laʻana, ua ʻike nā haʻawina e hiki i ka LL-37 ke hoʻokomo i loko o nā membrane cell o nā hua bacteria Gram-positive a me Gram-negative, e alakaʻi ana i ka piʻi ʻana o ka permeability o ka membrane cell, ka leakage o nā mea cell cell, a ma hope o ka make ʻana o ka bacteria.
Ka hana antibacterial ākea ākea:
Loaʻa i ka LL-37 ka hana antibacterial e kūʻē i nā ʻano maʻi maʻi kūʻē antibiotic. Hiki iā ia ke hana kū'ē i nā bacteria Gram-positive (e like me Staphylococcus aureus, Streptococcus, Enterococcus, etc.), Gram-negative bacteria (e like me Pseudomonas aeruginosa, Escherichia coli, Salmonella, a me nā mea'ē aʻe), a me nā maʻi bacterial'ē aʻe (e like me Mycoplasma, Ureaplasma, Mycobacterium.) 2] [ ʻO kēia hana antibacterial ākea ākea ka mea e loaʻa ai i ka LL-37 ka waiwai hoʻohana pono i ka hakakā ʻana i nā ʻano ʻano like ʻole o ka maʻi bacteria pale antibiotic.
Ke luku nei i ka biofilm i hana ʻia:
ʻO ka biofilm bacteria kekahi o nā kumu koʻikoʻi no ka pale ʻana i ka lāʻau o nā bacteria pathogenic. Hiki i ka peptide antibacterial LL-37 ke hoʻopau i ka biofilm i hoʻokumu ʻia, no laila e hōʻemi ana i ka pale ʻana i ka lāʻau lapaʻau. No ka laʻana, i ka maʻi prosthetic joint maʻi (PJI) ma hope o ka hoʻololi ʻana i ka hui ʻana, ʻo ke kūʻē ʻana i ka lāʻau o nā bacteria pathogenic i hana ʻia e ka biofilm bacterial e paʻakikī ai ka mālama ʻana. Eia naʻe, hiki i ka LL-37 ke pāʻani i ka hana antibacterial a me ka bacteriostatic maikaʻi ma ke kāohi ʻana i ka hana ʻana o ka biofilm a me ka luku ʻana i ka biofilm i hana ʻia.
Hoʻonui i ka hana antibacterial o nā antibiotics:
Ua hōʻike nā haʻawina he hopena synergistic ka LL-37 me kekahi mau lāʻau antibiotic. No ka laʻana, ke hoʻohana pū ʻia me ka amoxicillin clavulanic acid (AMC), hiki i ka LL-37 ke hoʻoikaika ikaika i ka hana antibacterial o AMC.
Puna:PubMed [6]
He aha nā noi no LL-37?
Hoʻoikaika i ka hana hou ʻana o ka iwi:
Ua hōʻike kekahi mau haʻawina he hopena maikaʻi ka peptide antibacterial LL-37 i ka hoʻōla hou ʻana o ka iwi. Kekahi noiʻi ua hōʻike i ke kanaka adipose-i loaʻa mesenchymal stem cell (hADSCs) i moʻomeheu me ka likeʻole concentrations o LL-37, a uaʻikeʻia i ka manaʻo o LL-37 i ka hopena i ka osteogenic hiki o hADSCs, hiki i ka piko ma 4μg / ml. Eia kekahi, ua hōʻike ka PSeD / hADSCs / LL-37 i nā waiwai osteogenic i ʻoi aku ka maikaʻi ma mua o ka PSeD / hADSCs, PSeD, a me nā scaffolds pūʻulu mana i ke kumu hoʻohālike defect rat calvarial, e hōʻike ana i kahi kiʻekiʻe kiʻekiʻe i ka hoʻōla hou ʻana o ka iwi clinical.
Ka hopena antibacterial:
Ka pale ʻana i ka nui o nā bacteria pathogenic:
Ua hoʻohana kekahi mau noiʻi i ke ʻano hoʻoheheʻe micro-double e hoʻoholo ai i ka palena haʻahaʻa haʻahaʻa (MIC) o ka peptide antibacterial LL-37 e kūʻē iā Escherichia coli, Salmonella, a me Staphylococcus aureus. Ua hōʻike ʻia nā hopena i loaʻa i ka LL-37 nā degere like ʻole o nā hopena inhibitory ma kēia mau bacteria pathogenic ʻekolu, me ka haʻahaʻa haʻahaʻa haʻahaʻa he 3.12, 1.56, a me 0.78μg/mL. Ua hōʻike ʻia ka hoʻāʻo kūpaʻa wela i ka hana maikaʻi o ka peptide antibacterial recombinant i nā wela kiʻekiʻe. Ua hōʻike ʻia nā hopena hoʻokolohua acid-base stability e loaʻa ana i ka LL-37 kekahi mau hana ma ka pae pH o 2.0 a 12.0, me ka hana maikaʻi loa ma ka pH o 5.0 a 6.0, a ʻo -20 ° C ke kūlana maikaʻi loa no ka mālama lōʻihi [3].
Ka hopena ma luna o nā maʻi maʻi kūʻē i ka antibiotic:
Ua aʻo kekahi poʻe i ka pono antibacterial o ka peptide antibacterial LL-37 a me nā nanoparticles kālā (AgNPs) kūʻē iā Staphylococcus aureus (S. aureus), he microorganism i loaʻa mau i nā maʻi pili biofilm. Ua hōʻike ʻia nā hopena ʻo LL-37 ka lāʻau antibacterial maikaʻi loa, me ka hōʻemi ʻana i ka helu colony ma mua o 4 logarithms. ʻO ka hoʻohālikelike, ʻoi aku ka maikaʻi o nā hopena o nā nanoparticles kālā a me nā lāʻau antibiotic maʻamau, me ka hōʻemi ʻana i ka helu colony ma lalo o 1 logarithm. ʻO ka lāʻau hoʻohui antibacterial me rifampicin i hoʻonui nui i ka hōʻemi logarithmic o AgNPs a me gentamicin, akā ʻoi aku ka haʻahaʻa ma mua o ka LL-37 i hoʻohana wale ʻia [4].
Hoʻohana i ka maʻi pulmonary:
Ua hōʻike ʻia nā haʻawina ua lilo ʻo Pseudomonas aeruginosa (PA) i mea paʻakikī koʻikoʻi no ka maʻi pulmonary a me ka ʻeha ʻeha. ʻO ka LL37 peptide he mea hana antibacterial maikaʻi e kūʻē i nā kuʻi PA, akā ua kaupalena ʻia kāna noi ma muli o kona hoʻomaʻemaʻe wikiwiki i vivo, nā pilikia biosafety, a me ka bioavailability haʻahaʻa. No laila, ua hoʻomohala ʻia kahi ʻōnaehana lawe nanodrug e hoʻemi ana i ka albumin ma muli o ka hoʻomaikaʻi ʻana i ka hana o LL37 e kūʻē iā PA in vivo ma o ka hana ʻana i nā paʻa disulfide intermolecular. Hiki ke hoʻopili pono ʻia ka Cationic LL37 ma o ka pilina electrostatic e hoʻoikaika i ka hopena antibacterial. Ua hōʻike ka LL37 peptide i ka hoʻokuʻu mau ʻana ma mua o 48 mau hola mai ka LL37 peptide nanoparticles (LL37 PNP), a ua ʻike ʻia kahi hopena antibacterial lōʻihi me ka piʻi ʻana o ka manawa incubation. I loko o kahi kiʻi ʻiole o ka maʻi pulmonary PA acute, LL37 PNP i hōʻemi nui i ka hōʻike ʻana o TNF-α a me IL-1β a me ka hōʻemi ʻana i ka ʻeha ʻeha. Hōʻike ia e hiki i ka LL37 PNP ke hoʻomaikaʻi maikaʻi i ka maʻi pulmonary PA a me ka pane inflammatory ma mua o ka peptide LL37 manuahi [3].
Hoʻoulu i ka hana antibacterial o nā platelets:
Ua hōʻike nā haʻawina e hiki i ka peptide antibacterial LL-37 ke hoʻoikaika i ka hana antibacterial o nā platelets kanaka. Ma hope o ka mālama ʻia ʻana o nā platelets me LL-37, ka ʻike ʻana o ka ʻili o nā mea loaʻa no ka ʻike ʻana i nā microorganisms (Toll-like receptors (TLRs) 2 a me -4, CD32, CD206, Dectin-1, CD35, LOX-1, CD41, CD62P, a me αIIbβ3 integrin) a me nā molekole e pili ana i ka hōʻike ʻana i ka CD8, CD8 antigentes (CD8 antigentes). Hoʻonui ʻia ka HLA-ABC), a ua hūnā ʻia nā molekole antibacterial: bactericidal/permeability-increasing protein (BPI), azurocidin, human neutrophil peptide (HNP) -1, a me myeloperoxidase. Unuhi pū lākou i ka azurocidin a hoʻonui i ka paʻa iā Escherichia coli, Staphylococcus aureus, a me Candida albicans. Eia kekahi, hiki i ka supernatant o nā platelets i mālama ʻia me LL-37 ke kāohi i ka ulu ʻana o Escherichia coli, a i ʻole nā platelets hiki ke hoʻohana i kā lākou LL-37 e kāohi i ka ulu ʻana o ka microbial [5].
Hoʻohana i nā ʻōnaehana lawe lāʻau
Ua hōʻike nā haʻawina i nā peptides antibacterial (AMPs) he papa hou o nā biomolecules me nā waiwai antibacterial ākea ākea a ua huki i ka manaʻo ma muli o ka piʻi wikiwiki ʻana o ke kū'ē antibiotic [6] . ʻO LL37 wale nō ka peptide antibacterial i loaʻa i ka cathelicidin i loaʻa i nā kānaka. Me ka noiʻi hohonu, ua hōʻike ʻo LL37 i nā hana olaola like ʻole, me ka hoʻoponopono ʻana i ka pane inflammatory, chemotaxis o nā cell immune, hoʻoikaika i ka ho'ōla ʻeha, a me ka osteogenesis, ka mea i paipai i nā ʻano noiʻi lapaʻau. Eia naʻe, ua kaupalena ʻia ka unuhi lapaʻau o LL37 e kona naʻau i ka degradation protease, hiki ke ʻona, bioavailability maikaʻi ʻole, a me nā mea ʻē aʻe.
I ka hopena, ma ke ʻano he peptide bioactive multifunctional, ua hōʻike ʻo LL-37 i ka mana nui i nā noi lapaʻau. Ma keʻano o ka hoʻouluʻana i ka iwi, ma o nā hopena synergistic o nā mīkini he nui e like me ka hoʻoikaikaʻana i kaʻokoʻa a me ka hana o ka osteoblast, nā hopena antibacterial, immunomodulation, a me ka hoʻouluʻana i ka angiogenesis, ua lawe mai ia i ka manaolana hou no ka hoʻoponoponoʻana i kaʻeha iwi. I ka wā e pili ana i ka maʻi bacteria antibiotic, ma ka luku pololei ʻana i ka membrane cell, ke kāohi ʻana i ka hoʻokumu ʻana o nā biofilms, a me ka synergizing me nā antibiotics, ua manaʻo ʻia e lilo ia i mea kaua ikaika e hoʻoponopono i ka pilikia o ka lāʻau bacteria. I nā ʻōnaehana hāʻawi lāʻau lapaʻau, ma ka hoʻolālā ʻana a me ka hoʻolālā ʻana i nā template peptide antibacterial, ke kūkulu ʻana i nā ʻōnaehana lawe lāʻau he nui, a me ka ʻimi ʻana i ka hoʻohana ʻana o nā lāʻau lapaʻau, hiki ke hoʻonui ʻia kona hopena lapaʻau. I ka pōkole, hiki i ka LL-37 ke hiki i nā ʻano he nui o nā noi lapaʻau, me ka hoʻoikaika ʻana i ka hoʻōla hou ʻana o ka iwi, nā hopena antibacterial, ka hoʻoulu ʻana i ka hana antibacterial o nā platelets, a me nā noi i nā ʻōnaehana lawe lāʻau.
No ka mea kakau
ʻO nā mea i ʻōlelo ʻia ma luna nei ua noiʻi ʻia, hoʻoponopono ʻia a hōʻuluʻulu ʻia e Cocer Peptides.
Mea kākau moʻolelo ʻepekema
He kanaka noiʻi ʻo Francisco J. Sanchez-Pena ma Universidad Autonoma Benito Juarez de Oaxaca (Autonomous Benito Juarez University of Oaxaca). Hoʻokumu ʻia i ka makahiki 1827, he hale aupuni koʻikoʻi kēia kulanui ma Oaxaca, Mexico, e hāʻawi ana i kahi ākea o nā papahana hoʻonaʻauao ma nā kula e like me ka ʻepekema kūlohelohe, ʻenekinia, humanities, a me ka ʻepekema pilikanaka.
ʻO ka noiʻi a Francisco J. Sanchez-Pena e kālele ana i ka Biochemistry & Molecular Biology, Microbiology, and Chemistry. ʻO kēia mau aʻo ʻana e pili ana i ke aʻo ʻana i nā kaʻina hana kemika i loko o nā mea ola, nā ʻano molekala a me nā hana, nā hiʻohiʻona microbial a me kā lākou pilina me ke kaiapuni, a me ka haku mele ʻana, nā waiwai, a me nā lula hoʻololi o nā mea kemika. He noi nui ka noiʻi ma kēia mau wahi i ka lāʻau lapaʻau, ka mahiʻai, ka ʻepekema kaiapuni, a me nā mea hou aku. Ua helu ʻia ʻo Francisco J. Sanchez-Pena ma ka ʻōlelo kuhikuhi [5].
