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1 ngwa (10 Vials)
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▎ LL-37 Ọdịdị
Isi mmalite: PubChem |
Usoro: LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES Ụdị mkpụrụ ndụ: C 205H 340N 60O53 Ibu ibu: 4493 g/mol Nọmba CAS: 154947-66-7 PubChem CID: 16198951 Synonyms: Cathelicidin;ropocamptide |
▎ Nchọpụta LL-37
Kedu ihe ndabere nyocha nke LL-37?
LL-37 bụ nke mbụ chọpụtara dị ka klas nke cationic obere peptide bekee na pupae nke silkworm Samia cynthia ricini site Swedish ọkà mmụta sayensị Boman HG na 1980. LL-37 bụ C-terminal peptide (CAMP, hCAP18) nke mmadụ cathelicidin antibacterial peptide na-arụ ọrụ dị mkpa na-arụ ọrụ na microbile eguzogide na-arụ ọrụ na microbile. chemotaxis, na-akwalite mmechi ọnya, na angiogenesis (Chen X, 2018). Antibacterial peptides dị n'ọtụtụ ebe na anụmanụ, osisi, na obere obere microorganisms, na ha bụ akụkụ dị mkpa nke mgbochi ebumpụta ụwa nke vertebrates. Dị ka protein nzuzo, LL-37 dị n'ọtụtụ akụkụ na anụ ahụ nke ahụ mmadụ. Mkpụrụ ndụ dị iche iche, gụnyere sel epithelial, keratinocytes, mkpụrụ ndụ mast, neutrophils, macrophages, na monocytes, nwere ike zoo ya. Ọtụtụ n'ime ụdị ọgwụ nje a nwere 37 - 39 'amino acid residues' ma nwee 0 cysteine . N'ihi ntọala siri ike na ọnọdụ N-terminal nke peptide antibacterial, ọ nwere ike ịmepụta ihe kwụsiri ike 'amphiphilic helical structure'. Pentide antibacterial LL-37 nwekwara ihe nhazi α-helical amphiphilic. N'ihi ọrụ ya nke igbu nje bacteria na-akpata, a na-akpọ ya peptide antibacterial. Ihe '37' dị n'aha LL-37 nwere ike ịmetụta ọnụ ọgụgụ nke ihe fọdụrụ na amino acid ya. N'otu oge ahụ, a makwaara ya dị ka cathelicidin na ropocamptide.
Kedu ihe bụ usoro nke LL-37 megide nje bacteria na-eguzogide ọgwụ?
Na-emebi akpụkpọ ahụ nke nje bacteria:
LL-37 nwere ike itinye n'ime akpụkpọ ahụ nke nje bacteria, karịsịa na-enwe mmetụta na-emebi emebi na akpụkpọ ahụ cell nwere phosphatidylglycerol (DPPG). Ọ ga-akpaghasị nhazi nke akpụkpọ ahụ nke nje bacteria, si otú a na-enwe mmetụta nje bacteria [1] . Dịka ọmụmaatụ, nchọpụta achọpụtala na LL-37 nwere ike ịtinye n'ime akpụkpọ anụ cell nke ma nje bacteria Gram-positive na Gram-negative, na-eduga n'ịbawanye ike nke akpụkpọ ahụ cell, nkwụsị nke ọdịnaya cell, na n'ikpeazụ ọnwụ nke nje bacteria.
Arụ ọrụ nje antibacterial sara mbara:
LL-37 nwere ọrụ antibacterial megide ọtụtụ nje bacteria na-eguzogide ọgwụ. Ọ nwere ike ime ihe megide nje bacteria Gram-positive (dị ka Staphylococcus aureus, Streptococcus, Enterococcus, wdg), nje bacteria na-adịghị mma (dị ka Pseudomonas aeruginosa, Escherichia coli, Salmonella, wdg), na ndị ọzọ na-akpata nje bacteria (dị ka Mycoplasma, Ureaumplasma, wdg) [bacteria] . Ọrụ nje bacteria na-eme ka LL-37 nwee uru ngwa n'ịlụso ụdị nje bacteria dị iche iche ọgụ.
Na-ebibi biofilm a kpụrụ:
Bacterial biofilm bụ otu n'ime ihe ndị dị mkpa maka iguzogide ọgwụ nke nje bacteria. The antibacterial peptide LL-37 nwere ike ibibi biofilm kpụrụ, si otú ibelata ọgwụ iguzogide nje bacteria. Dịka ọmụmaatụ, na ọrịa nkwonkwo prosthetic (PJI) mgbe a na-anọchi anya nkwonkwo artificial, nkwụsị ọgwụ nke nje bacteria na-akpata site na nje biofilm na-eme ka ọgwụgwọ ahụ sie ike. Otú ọ dị, LL-37 nwere ike ịrụ ọrụ antibacterial na bacteriostatic dị irè site na igbochi nhazi nke biofilm na ibibi biofilm kpụrụ.
Na-akwalite ọrụ antibacterial nke ọgwụ nje:
Nnyocha egosiwo na LL-37 nwere mmetụta synergistic na ụfọdụ ọgwụ nje. Dịka ọmụmaatụ, mgbe ejiri ya na amoxicillin clavulanic acid (AMC), LL-37 nwere ike ịkwalite ọrụ antibacterial nke AMC.
Isi mmalite: PubMed [6]
Kedu ihe bụ ngwa maka LL-37?
Na-akwalite mmeghari ọkpụkpụ:
Ụfọdụ ọmụmụ egosila na peptide antibacterial LL-37 nwere mmetụta dị mma na ọkpụkpụ ọkpụkpụ. Ụfọdụ nnyocha egosiwo na mkpụrụ ndụ mesenchymal mesenchymal adipose (hADSCs) na-emepụta ihe dị iche iche nke LL-37, a chọpụtakwara na ntinye nke LL-37 nwere mmetụta na ikike osteogenic nke hADSCs, na-eru elu na 4μg / ml. Tụkwasị na nke ahụ, PSeD / hADSCs / LL-37 jikọtara scaffold gosipụtara ihe ndị dị elu nke osteogenic karịa PSeD / hADSCs, PSeD, na ndị na-achịkwa scaffolds na ụdị ntụpọ calvarial nke oke, na-egosi ikike dị elu n'ịmụghachi ọkpụkpụ ụlọ ọgwụ.
Mmetụta antibacterial:
Mgbochi nke ọtụtụ nje bacteria:
Ụfọdụ nchọcha ji usoro dilution micro-double iji chọpụta opekempe ihe mgbochi mgbochi (MIC) nke peptide antibacterial LL-37 megide Escherichia coli, Salmonella, na Staphylococcus aureus. Nsonaazụ gosiri na LL-37 nwere ogo dị iche iche nke inhibitory mmetụta na nje bacteria atọ ndị a, nke kacha nta na-egbochi ya bụ 3.12, 1.56, na 0.78μg/mL, n'otu n'otu. Nnwale nkwụsi ike nke thermal gosiri na recombinant antibacterial peptide ka nwere ezigbo ọrụ na oke okpomọkụ. Nsonaazụ nkwụsi ike nke acid-base gosiri na LL-37 nwere ụfọdụ ọrụ na pH nke 2.0 ruo 12.0, yana ọrụ kachasị mma na pH nke 5.0 ruo 6.0, na -20 Celsius bụ ọnọdụ kachasị mma maka nchekwa ogologo oge [3]..
Mmetụta na nje bacteria na-eguzogide ọgwụ:
Ụfọdụ ndị mmadụ mụrụ irè antibacterial nke peptide peptide LL-37 na ọlaọcha nanoparticles (AgNPs) megide Staphylococcus aureus (S. aureus), microorganism a na-ahụkarị na ọrịa ndị metụtara biofilm. Nsonaazụ gosiri na LL-37 bụ onye na-ahụ maka nje nje kacha arụ ọrụ nke ọma, yana mbelata ọnụ ọgụgụ obodo karịrị 4 logarithms. N'ụzọ dị iche, mmetụta nke nanoparticles ọlaọcha na ọgwụ nje ndị a na-emekarị dị njọ karị, na mbelata ọnụ ọgụgụ ndị obodo na-erughị 1 logarithm. Usoro ọgwụgwọ nje bacteria na rifampicin mụbara mbelata logarithmic nke AgNPs na gentamicin, mana ọ ka dị obere karịa nke LL-37 ejiri naanị ya [4]..
Ngwa n'ọrịa akpa ume:
Nnyocha egosiwo na Pseudomonas aeruginosa (PA) aghọwo ihe ịma aka ngwa ngwa maka ọrịa pulmonary na mmerụ ahụ nku ume. The LL37 peptide bụ ihe na-arụ ọrụ antibacterial dị irè megide ụdị PA, mana ngwa ya nwere oke n'ihi mkpochapụ ngwa ngwa na vivo, nsogbu biosafety, yana obere bioavailability. Ya mere, usoro nnyefe nanodrug nke dabeere na albumin na-ebelata-enwe mmetụta ka emepụtara iji melite arụmọrụ nke LL37 megide PA in vivo site na ịmepụta njikọ disulfide intermolecular. Enwere ike ịtinye Cationic LL37 nke ọma site na mmekọrịta electrostatic iji gosipụta mmetụta nje na-eme ka ọ dịkwuo mma. LL37 peptide gosipụtara ntọhapụ na-adịgide adịgide nke ihe karịrị awa 48 site na LL37 peptide nanoparticles (LL37 PNP), na mmetụta nje bacteria gbatịrị agbatị ka achọpụtara na mmụba nke oge ntinye. N'ụdị òké nke nnukwu ọrịa pulmonary PA, LL37 PNP belatara okwu TNF-a na IL-1β nke ukwuu ma belata mmerụ ahụ nku ume. Ọ na-egosi na LL37 PNP nwere ike imeziwanye ọrịa pulmonary PA nke ọma na nzaghachi mkpali na-esote karịa peptide LL37 efu [3].
Na-arụ ọrụ antibacterial nke platelet:
Nnyocha e mere egosiwo na peptide antibacterial LL-37 nwere ike ime ka ọrụ nje bacteria na-arụ ọrụ platelet mmadụ. Mgbe a gwọchara platelet na LL-37, ngosipụta elu nke ndị na-anabata ihe maka ịmata microorganisms (Toll-like receptors (TLRs) 2 na -4, CD32, CD206, Dectin-1, CD35, LOX-1, CD41, CD62P, na αIIbβ3 integrin, na ụmụ irighiri ihe jikọrọ ya na CD8 na antigens. HLA-ABC) na-abawanye, na-ezobekwa ụmụ irighiri ihe nje bacteria: bactericidal / permeability-protein na-abawanye protein (BPI), azurocidin, peptide neutrophil mmadụ (HNP) -1, na myeloperoxidase. Ha na-atụgharịkwa azurocidin ma kwalite njikọ na Escherichia coli, Staphylococcus aureus, na Candida albicans. Tụkwasị na nke ahụ, ihe dị elu nke platelet a na-emeso ya na LL-37 nwere ike igbochi uto nke Escherichia coli, ma ọ bụ platelet nwere ike iji LL-37 ha egbochi uto microbial [5]..
Ngwa na usoro nnyefe ọgwụ
Nnyocha ekwupụtala na peptides antibacterial (AMPs) bụ klas ọhụrụ nke biomolecules nke nwere ihe ndị na-egbu egbu na-egbuke egbuke ma dọta uche n'ihi mmụba ngwa ngwa nke mgbochi nje [6] . LL37 bụ naanị cathelicidin sitere na peptide antibacterial enwetara n'ime mmadụ. Site na nyocha miri emi, LL37 egosila ọrụ dị iche iche nke ndu, gụnyere ịhazi nzaghachi mkpali, chemotaxis nke mkpụrụ ndụ na-alụso ọrịa ọgụ, na-akwalite ọgwụgwọ ọnya, na osteogenesis, bụ nke akwadola ọtụtụ ngwa ụlọ ọgwụ. Otú ọ dị, nsụgharị ụlọ ọgwụ nke LL37 na-ejedebe site na uche ya na mmebi nke protease, nsị nwere ike ime, adịghị ike bioavailability, wdg. Usoro nnyefe dị iche iche, gụnyere ígwè nanoparticles, polymer material, na lipid-based system, ka ewebatala iji nweta ngwa ọgwụgwọ.
N'ikpeazụ, dị ka peptide multifunctional bioactive, LL-37 egosila nnukwu ikike na ngwa ụlọ ọgwụ. N'ihe gbasara ịkwalite ọkpụkpụ ọkpụkpụ, site na mmetụta synergistic nke ọtụtụ usoro dị ka ịkwalite ọdịiche na ọrụ osteoblast, mmetụta antibacterial, immunomodulation, na ịkwalite angiogenesis, ọ na-eweta olileanya ọhụrụ maka nrụzi mmerụ ọkpụkpụ. Mgbe a na-emeso nje bacteria na-eguzogide ọgwụ, site n'ibibi akpụkpọ ahụ cell ozugbo, igbochi mmepụta biofilms, na iji ọgwụ nje emekọrịta ihe, a na-atụ anya na ọ ga-abụ ngwá agha siri ike iji dozie nsogbu nke nje bacteria na-eguzogide ọgwụ. Na usoro nnyefe ọgwụ, site n'ichepụta na ịkwalite ndebiri peptide antibacterial, na-ewu ọtụtụ usoro nnyefe ọgwụ, na ịgagharị ngwa ngwa nke ọgwụ ọjọọ, enwere ike ịkwalite mmetụta ọgwụgwọ ya. Na nkenke, LL-37 nwere ikike n'ọtụtụ akụkụ nke ngwa ụlọ ọgwụ, gụnyere ịkwalite mmeghari ọkpụkpụ, mmetụta antibacterial, rụọ ọrụ antibacterial ọrụ nke platelet, na ngwa na usoro nnyefe ọgwụ.
Banyere Onye dere ya
Ihe niile a kpọtụrụ aha n'elu bụ Cocer Peptides na-enyocha, dezie ma chịkọta ya.
Onye ode akwụkwọ akụkọ sayensị
Francisco J. Sanchez-Pena bụ onye nyocha na Universidad Autonoma Benito Juarez de Oaxaca (Mahadum Benito Juarez nke Oaxaca kwụụrụ onwe ya). N'ịbụ onye guzosiri ike na 1827, mahadum a bụ ụlọ ọrụ ọha na eze dị mkpa na Oaxaca, Mexico, na-enye ọtụtụ mmemme agụmakwụkwọ n'ọhịa dị ka sayensị okike, injinia, mmadụ na sayensị mmekọrịta.
Nchọpụta Francisco J. Sanchez-Pena lekwasịrị anya na Biochemistry & Molecular Biology, Microbiology, and Chemistry. Ọzụzụ ndị a gụnyere ọmụmụ nke usoro kemịkalụ n'ime ihe ndị dị ndụ, ihe owuwu na ọrụ molekụla, njirimara microbial na mmekọrịta ha na gburugburu ebe obibi, yana nhazi, akụrụngwa, na iwu mgbanwe nke ihe kemịkal. Nnyocha na mpaghara ndị a nwere nnukwu ngwa na ọgwụ, ọrụ ugbo, sayensị gburugburu ebe obibi, na ndị ọzọ. Francisco J. Sanchez-Pena depụtara na ntụaka ntụaka [5].
