HCG 5000 ku

▎ Tsarin HCG

▎ Binciken HCG

Menene asalin bincike na hCG?

hCG yana taka muhimmiyar rawa a cikin maganin haihuwa, gami da kiyaye ciki da wuri da kuma haifar da ovulation a cikin taimakon fasahar haihuwa. Ana fitar da hCG na halitta da farko daga fitsarin mata masu juna biyu, yana nuna gazawa a cikin wadata da tabbatar da tsabta. Wannan ya kasa biyan buƙatun asibiti girma, yana buƙatar haɓaka hCG na roba don samar da ingantaccen tushen magani.

Ci gaba a cikin fasahar injiniyan kwayoyin halitta sun ba da damar cloning da bayyana kwayar halittar hCG, da kafa tushen fasaha don bincike na hCG na roba. Ta hanyar gabatar da kwayar halittar hCG a cikin sel masu masauki kamar yisti ko hamster ovary na kasar Sin (CHO), za a iya samun nasarar sake bayyana hCG. HCG na roba yana ba da samfurori mafi tsabta don nazarin tsari da aikin aiki, yana sauƙaƙe fahimtar zurfin fahimtar hanyoyin aikinsa da kuma ba da damar haɓaka hanyoyin hanyoyin warkewa mafi inganci. Bugu da ƙari, ana iya amfani da hCG na roba don shirya reagents na bincike, haɓaka daidaiton ganewar ciki da kuma kula da yanayin da ke da alaƙa.

Menene tsarin aiki don hCG?

Mechanism a cikin kiyaye ciki: hCG da farko ana samar da su ta hanyar bambance-bambancen sel syncytiotrophoblast kuma yana aiki azaman siginar amfrayo mai mahimmanci don ɗaukar ciki. Yana kunna ɓangarorin sigina da yawa ta hanyar ɗaure ga hormone luteinizing/ ɗan adam chorionic gonadotropin receptor (LHCGR). Binciken Nwabuobi C yana nuna cewa ta hanyar hulɗar kai tsaye ko kai tsaye tare da mai karɓar beta mai girma (TGFβR), yana kunna hanyoyin siginar uwa kamar Smad2, protein kinase C (PKC), da / ko protein kinase A (PKA). A cikin inganta angiogenesis na endothelial na mahaifa, hCG yana taimakawa wajen samar da isasshen abinci mai gina jiki da oxygen don ci gaban amfrayo; A cikin kula da ƙwayar mahaifa na myometrial quiescence, yana haifar da tsayayyen yanayin intrauterine wanda ya dace don dasa amfrayo da haɓakawa; A cikin haɓaka ƙa'idodin rigakafi a cikin mahaɗar mahaifa- tayi, hCG yana daidaita tsarin rigakafi na uwa don hana ƙin yarda da amfrayo, yana tabbatar da samun ci gaba cikin nasara ^[1].

Hanyoyin aiki don inganta karɓar karɓar endometrial: A cikin gwaje-gwajen vivo ta amfani da samfurin linzamin kwamfuta na mahaifa (EID) da kuma ƙwararrun epithelial na ɗan adam (EECs) sun bayyana cewa hCG yana haɓaka karɓar endometrial a cikin mice EID. hCG yana daidaita ma'aunin miR-126-3p/PIK3R2 ta hanyar haɓaka maganganun miR-126-3p da hana PIK3R2, yayin da miR-126-3p ke hari PIK3R2. An haɓaka haɓakar EEC bayan maganin hCG amma an hana shi lokacin da aka lalata miR-126-3p. Duka a cikin vivo da gwaje-gwajen in vitro sun tabbatar da cewa hCG yana haɓaka karɓuwar endometrial kuma yana sauƙaƙe dasawa tayi ta hanyar kunna hanyar PI3K/Akt/eNOS ta hanyar miR-126-3p/PIK3R2 axis ^[2].

Hoto na 1 hCG ya inganta karɓowar endometrial a cikin berayen EID ^[2].

Tasirin glycosylation akan hanyoyin hormonal: Nazarin kan nau'in glycosylated na hCG ya nuna cewa cire ragowar sukari daban-daban daga abubuwan da suka samo asali na hCG daban-daban yana shafar ikon ɗaure su zuwa ƙwayoyin Leydig na bera da ikon su na haɓaka haɓakar testosterone da cyclic AMP (cAMP). Tare da kau da jeri na sialic acid, galactose, N-acetylglucosamine, da ragowar mannose, ingantaccen maganin hormone da ake buƙata don haɓaka samar da steroid ya karu a hankali, yayin da ƙarfin haɓaka tarin cAMP ya ragu sosai. Ƙananan matakan glycosidase-masu maganin hormone sun nuna tasirin ƙari lokacin da aka bincikar su tare da hCG don ikon su na ƙarfafa haɗin testosterone. Duk da haka, waɗannan abubuwan da aka samo asali sun kasance masu hanawa na hCG-induced cAMP tarawa, suna ba da shawarar cewa cirewar glycosylation kadan yana rinjayar daurin hormone-cell yayin da yake rage ikon da aka daure hormones don kunna adenylate cyclase. Wannan yana ƙara jaddada mahimmancin rawar glycosylation a cikin fassarar sigina a cikin tsarin aikin hCG ^[3].

Menene aikace-aikacen hCG?

ganewar asali na ciki na farko: Ana amfani da hCG na roba don shirya abubuwan ganowa. A cikin aikin asibiti, ana ƙayyade ciki ta hanyar auna matakan hCG a cikin fitsari ko jinin mace. Kusan kwanaki 6-7 bayan hadi, ƙwayoyin trophoblast suna fara ɓoye hCG. Yayin da ciki ke ci gaba, matakan hCG a cikin jini da fitsari suna tashi da sauri. Yin amfani da hCG na roba a matsayin ma'auni yana ba da damar daidaitaccen kafa daidaitaccen madaidaicin lanƙwasa don gano reagents, yana ba da damar ƙididdige ƙimar abun ciki na hCG a cikin samfuran. Misali, igiyoyin gwajin ciki na gama-gari sun dogara da fasahar immunochromatography, inda ƙwayoyin rigakafi da aka shirya daga hCG na roba musamman ke ɗaure hCG a cikin fitsari, tare da halayen launi na nuna halin ciki. Yawanci, bayan lokacin da aka rasa, mata na iya amfani da irin waɗannan hanyoyin don tantance juna biyu na farko, suna ba da tushe don kulawa da kulawa da haihuwa na gaba ^[1].

Kula da yanayin da ke da alaƙa da juna biyu: Lokacin daukar ciki, bin diddigin canje-canje masu ƙarfi a cikin matakan hCG na uwa yana taimakawa wajen ganowa da lura da cututtukan da ke da alaƙa da juna biyu. Misali, a cikin ciki na ectopic, inda kwai da aka haɗe a waje da rami na mahaifa, ƙwayoyin trophoblastic suna haɓaka da rashin daidaituwa. Wannan yana haifar da ƙananan ɓoyewar hCG idan aka kwatanta da ciki na ciki na al'ada da kuma tsawon lokaci mai tsawo. Ci gaba da lura da matakan hCG na jini, haɗe tare da gwaje-gwajen duban dan tayi, yana sauƙaƙe gano ciki da wuri na ectopic ciki. Wannan yana ba da izinin shiga tsakani da magani na lokaci, yana hana rikitarwa mai tsanani. Bugu da ƙari, ga cututtuka na trophoblastic irin su hydatidiform mole, matakan hCG yawanci suna nuna haɓaka mara kyau. Bayan jiyya, ci gaba da saka idanu na hCG na iya tantance tasirin warkewa da gano sake dawowa. Matsayin haɓakawa ko haɓaka matakan hCG suna nuna yiwuwar saura cuta ko sake dawowa, yana buƙatar ƙarin bincike da sa baki ^[1].

Menene ci gaban gwaji game da hCG?

Magungunan rigakafin hana haihuwa sun haɓaka ta amfani da cikakken β-subunit na hCG a matsayin immunogen da sashin peptide na 37-amino acid (C-terminus 109-145) yayin da immunogen ya wuce ƙayyadaddun guba da gwaje-gwajen aminci kuma sun kammala gwajin asibiti na Phase I da Phase II. A cikin gwajin asibiti na kwatankwacin lokaci na 1 da ya ƙunshi mata 116 masu aikin sa kai waɗanda aka yi wa aikin tubal ligation, an gwada tsarin rigakafin tushen β-hCG guda uku. Sakamako ya nuna cewa duk matan da aka yi wa alurar riga kafi sun sami ƙwayoyin rigakafi daga hCG da tetanus, wanda ke nuna waɗannan alluran rigakafin yadda ya kamata suna ƙarfafa martanin rigakafi na ɗan adam ^[4]..

Don samun nasarar hana haihuwa da kuma magance cututtukan da ke da alaka da hormone, masu bincike sun kirkiro maganin alurar rigakafi da ke nufin hCG. An shirya immunogens ta hanyar haɗa peptides na roba wanda ke wakiltar tsarin farko na hCG β-subunit zuwa masu ɗaukar furotin. Yayin haɓakawa, masu bincike sun haɗa nau'ikan peptides masu tsayi daban-daban waɗanda aka zaɓa daga yankin C-terminal na β subunit kuma sun gwada ikon su na haifar da ƙwayoyin rigakafi waɗanda ke da ikon amsawa tare da hCG da kawar da ayyukan sa a cikin vivo. A ƙarshe, an zaɓi ɓangaren peptide na 37-amino acid wanda ke wakiltar yankin C-terminal na β subunit azaman antigen. An zaɓi toxoid diphtheria a matsayin mai ɗaukar hoto, wanda ke haifar da shirye-shiryen rigakafin samfur na farko. Following trials across multiple species, this vaccine successfully induced significant antibody levels against hCG, and a marked reduction in fertility was observed in immunized baboons. Wannan ya ba da sabbin fahimta da hanyoyin da za a iya bi don sarrafa haifuwar ɗan adam da kuma maganin cututtukan da ke da alaƙa ^[5].

Kammalawa

A matsayin maɓalli na glycoprotein hormone, hCG yana taka muhimmiyar rawa a cikin tsarin haihuwa: a cikin mata, yana aiki ta hanyar ɗaure mai karɓa na hormone / chorionic gonadotropin receptor (LHCGR) don ci gaba da daukar ciki da wuri (goyon bayan fitowar luteal progesterone da samar da ingantaccen yanayin mahaifa), inganta yanayin balaga, haɓaka haɓakar haɓakar mahaifa da haɓaka haɓakar haɓakar haɓakar mahaifa. shigarwa. A cikin maza, yana ƙarfafa ƙwayoyin Leydig don haɗawa da ɓoye testosterone, yana taimakawa ci gaban gabobin haihuwa da spermatogenesis.

Game da Marubuci

Abubuwan da aka ambata a sama duk bincike ne, gyara su kuma Cocer Peptides ne suka haɗa su.

Mawallafin Jaridar Kimiyya

Nwabuobi C mai bincike ne da ke mai da hankali kan fannin nazarin gonadotropin chorionic na ɗan adam (hCG). Binciken su ya fi mayar da hankali kan nazarin ayyukan nazarin halittu na hCG da kuma bincika alaƙarta da aikace-aikacen asibiti. Nwabuobi C sau da yawa yana ɗaukar haɗin hanyoyin gwaji kamar dabarun ilimin ƙwayoyin cuta da nazarin samfurin asibiti don gudanar da bincike mai zurfi, da nufin zurfafa fahimtar hanyoyin ilimin halittar jiki na hCG da ƙimar sa mai amfani a cikin yanayin asibiti. An jera Nwabuobi C a cikin ambaton ambaton [1].

▎ Abubuwan da suka dace

[1] Nwabuobi C, Arlier S, Schatz F, Guzeloglu-Kayisli O, Lockwood CJ, Kayisli UA. hCG: Ayyukan Halittu da Aikace-aikacen asibiti. Jarida ta Duniya na Kimiyyar Kwayoyin Halitta 2017; 18 (10).DOI: 10.3390/ijms18102037.

[2] Wang W, Ge L, Zhang LL, et al. Mechanism na gonadotropin chorionic ɗan adam a cikin karɓan endometrial ta hanyar miR-126-3p/PI3K/Akt/eNOS axis. Kaohsiung Journal of Medical Sciences 2023; 39(5): 468-477.DOI: 10.1002/kjm2.12672.

[3] Moyle WR, Bahl OP, März L. Matsayin carbohydrate na gonadotropin chorionic na mutum a cikin tsarin aikin hormone. Jaridar Kimiyyar Halittu 1975; 250(23): 9163-9169.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=172504&query_hl=1.

[4] Talwar GP, Hingorani V, Kumar S, et al. Gwajin asibiti na mataki na 1 tare da tsari guda uku na rigakafin cutar gonadotropin chorionic na ɗan adam. Maganin hana haihuwa 1990; 41(3): 301-316.DOI: 10.1016/0010-7824 (90)90071-3.

[5] Stevens VC. Amfani da peptides na roba a matsayin immunogens don haɓaka maganin rigakafin gonadotropin chorionic na ɗan adam. Ciba Found Symp 1986; 119: 200-225.DOI: 10.1002/9780470513286.ch12.

DUKKANIN LABARI DA BAYANIN KYAUTATAWA DA AKE BAYAR A WANNAN SHAFIN KAWAI DOMIN WATSA BAYANI NE DA MANUFOFIN ILIMI.  

Kayayyakin da aka bayar akan wannan gidan yanar gizon an yi niyya ne kawai don binciken in vitro. Binciken in vitro (Latin: *a cikin gilashi*, ma'ana a cikin gilashin gilashi) ana gudanar da shi a wajen jikin mutum. Waɗannan samfuran ba magunguna ba ne, Hukumar Abinci da Magunguna ta Amurka (FDA) ba ta amince da su ba, kuma dole ne a yi amfani da su don hanawa, magani, ko warkar da kowane yanayi na likita, cuta, ko cuta. Doka ta haramta shigar da waɗannan samfuran cikin jikin mutum ko dabba ta kowace hanya.