HCG 5000iu

HCGStructure Imiterere ya

Research Ubushakashatsi bwa HCG

Ni ubuhe bushakashatsi bwakozwe na hCG?

HCG igira uruhare runini mubuvuzi bwimyororokere, harimo gukomeza gutwita hakiri kare no gutera intanga ngabo mu buhanga bw’imyororokere ifashwa. HCG isanzwe ikurwa cyane cyane mu nkari z'abagore batwite, bikagaragaza aho bigarukira mu gutanga no kwizerwa. Ibi binanirwa kuzuza ibyifuzo byubuvuzi bikura, bisaba iterambere rya hCG ya syntetique kugirango itange isoko ihamye kandi yizewe.

Iterambere mu buhanga bwubuhanga bwa genetike ryashoboje gukoroniza no kwerekana gene ya HCG, rishyiraho urufatiro rwa tekiniki yubushakashatsi bwa hCG. Mu kwinjiza gene ya HCG mu ngirabuzimafatizo nk'umusemburo cyangwa ingirabuzimafatizo ya hamster ovary (CHO), imvugo ya recombinant ya HCG irashobora kugerwaho. Synthetic hCG itanga urugero rwiza kubushakashatsi bwimiterere nuburyo bukora, byoroha gusobanukirwa byimazeyo nuburyo bwibikorwa kandi bigafasha iterambere ryuburyo bwiza bwo kuvura. Byongeye kandi, synthique hCG irashobora gukoreshwa mugutegura reagente yo kwisuzumisha, ikongerera ukuri kwisuzumisha no gutwita.

Ni ubuhe buryo bwo gukora kuri hCG?

Uburyo bwo gukomeza gutwita: HCG ikorwa cyane cyane na selile syncytiotrophoblast itandukanye kandi ikora nk'ikimenyetso gikomeye cyo gusama gikenewe mugukomeza gutwita. Ikora kasike nyinshi zerekana guhuza imisemburo ya luteinizing / reseptor ya chorionic gonadotropin reseptor (LHCGR). Ubushakashatsi bwakozwe na Nwabuobi C bwerekana ko binyuze mu mikoranire itaziguye cyangwa itaziguye n’impinduka zikura zikura beta reseptor (TGFβR), ikora inzira yerekana ibimenyetso byababyeyi nka Smad2, protein kinase C (PKC), na / cyangwa protein kinase A (PKA). Mu guteza imbere uterine endoteliyale angiogenezi, HCG ifasha gutanga imirire ihagije na ogisijeni kugirango ikure neza; Mugukomeza nyababyeyi ya myometrial quiescence, itanga ibidukikije bihamye byimbere mu nda bifasha gutera urusoro niterambere; Mu guteza imbere ubudahangarwa bw'umubiri ku isura y’umubyeyi n’uruhinja, hCG ihindura gahunda y’umubiri w’ababyeyi kugira ngo irinde kwanga urusoro, bituma inda igenda neza ^..

Uburyo bwibikorwa mugutezimbere kwakirwa kwimyanya ndangagitsina: Mubushakashatsi bwa vivo hakoreshejwe uburyo bwimbeba yimbeba (EID) yimbeba yimbeba hamwe na selile ya epiteliyale yumuntu (EECs) byagaragaje ko hCG yongerera ubushobozi bwimikorere yimbeba za EID. hCG igenga miR-126-3p / PIK3R2 mu guteza imbere imvugo ya miR-126-3p no guhagarika PIK3R2, mugihe miR-126-3p yibasiye PIK3R2. Ikwirakwizwa rya EEC ryongerewe imbaraga nyuma yo kuvura hCG ariko ryabujijwe igihe miR-126-3p yagabanijwe. Haba muri vivo ndetse no mubushakashatsi bwa vitro byemeje ko hCG yongerera ubushobozi bwa endometrale kandi ikorohereza insoro mugukoresha inzira ya PI3K / Akt / eNOS ikoresheje inzira ya miR-126-3p / PIK3R2 ^[2].

Igishushanyo 1 hCG cyanonosoye imitekerereze ya endometrale mu mbeba za EID ^[2].

Ingaruka za glycosylation ku mikorere ya hormone: Ubushakashatsi bwakozwe ku miterere ya glycosilated ya hCG bwerekana ko kuvanaho ibisigazwa bitandukanye by’isukari biva mu nkomoko ya HCG bigira ingaruka zitandukanye ku bushobozi bwabo bwo guhuza ingirabuzimafatizo za Leydig ndetse n’ubushobozi bwabo bwo gukangura testosterone na synthèque AMP (cAMP). Hamwe no kuvanaho urukurikirane rwa acide sialic, galactose, N-acetylglucosamine, hamwe n ibisigisigi bya mannose, igipimo cyiza cya hormone gisabwa kugirango umusaruro wa steroid ukomeze kwiyongera buhoro buhoro, mugihe ubushobozi bwo gukangura cAMP bwagabanutse cyane. Inkomoko ya glycosidase ivura imisemburo ikomoka ku misemburo yerekanaga ingaruka ziyongera iyo zisesenguwe na hCG kubushobozi bwazo bwo gukangura testosterone. Nyamara, ibiyikomokaho byari imbaraga zikomeye zo gukwirakwiza cAMP iterwa na hCG, byerekana ko kuvana glycosylation bigira ingaruka nke cyane ku guhuza imisemburo ya selile mu gihe bigabanya ubushobozi bw’imisemburo ihambiriye gukora adenylate cyclase. Ibi birashimangira kandi uruhare rukomeye rwa glycosylation mugukwirakwiza ibimenyetso muburyo bwa hCG bwibikorwa ^[3].

Ni ubuhe buryo bukoreshwa muri hCG?

Gusuzuma hakiri kare gutwita: Synthetic hCG ikoreshwa mugutegura reagent. Mubikorwa byubuvuzi, gutwita bigenwa no gupima urugero rwa HCG mu nkari zumugore cyangwa mumaraso. Hafi yiminsi 6-7 nyuma yo gusama, selile trophoblast itangira gusohora hCG. Iyo geste igenda itera imbere, urugero rwa HCG mumaraso ninkari byiyongera vuba. Gukoresha sintetike ya HCG nkibisanzwe bituma hashyirwaho neza umurongo usanzwe wo gutahura reagent, bigafasha kugereranya neza ibintu bya hCG mubitegererezo. Kurugero, ibipimisho bisanzwe byo gutwita bishingiye ku buhanga bwa immunochromatografiya, aho antibodies zateguwe kuva synthique hCG zihuza cyane na HCG mu nkari, hamwe nibara ryerekana ibara ryerekana inda. Ubusanzwe, nyuma yigihe cyabuze, abagore barashobora gukoresha ubwo buryo mugupima mbere yo gutwita, bigatanga urufatiro rwo kwita no kubyara nyuma yo kubyara ^[1].

Gukurikirana imiterere ijyanye no gutwita: Mugihe cyo gutwita, gukurikirana impinduka zikomeye mubyiciro byababyeyi ba HCG bifasha mugupima no gukurikirana indwara zitandukanye ziterwa no gutwita. Kurugero, mugihe cyo gutwita kwa ectopique, aho intanga ngore yatewe hanze ya nyababyeyi, selile trophoblastique ikura bidasanzwe. Ibi bivamo gusohora kwa HCG ugereranije no gutwita bisanzwe kwa intrauterine nigihe kinini cyo kwikuba kabiri. Gukomeza gukurikirana urwego rwamaraso ya HCG, hamwe no gupima ultrasound, byorohereza kumenya hakiri kare inda za ectopique. Ibi bituma habaho gutabara no kuvurwa mugihe, birinda ingorane zikomeye. Byongeye kandi, kuburwayi bwa trophoblastique nka hydatidiform mole, urwego rwa HCG rusanzwe rwerekana ubutumburuke budasanzwe. Nyuma yubuvuzi, gukurikirana hCG guhoraho birashobora gusuzuma imikorere yubuvuzi no kumenya ko byongeye kubaho. Gukomeza kuzamuka cyangwa kuzamuka kwa HCG byerekana indwara zishobora kubaho cyangwa gusubira inyuma, bisaba ko hakorwa iperereza no gutabara ^[1].

Ni ubuhe buryo bwo kugerageza bwerekeranye na hCG?

Inkingo zo kuboneza urubyaro zakozwe hifashishijwe β-subunit yuzuye ya hCG nka immunogen hamwe na peptide ya aside amine 37 (C-terminus 109-145) kuko immunogen yatsinze ibizamini by’uburozi n’umutekano kandi irangiza ibizamini by’amavuriro Icyiciro cya mbere n’icyiciro cya II. Mu igereranya ry’icyiciro cya mbere cy’amavuriro cyitabiriwe n’abakorerabushake 116 b’abakobwa bari barangije kwanduza igituba, hapimwe urukingo rwa gatatu β-hCG. Ibisubizo byerekanye ko abagore bose bakingiwe bakoze antibodiyide zirwanya hCG na tetanusi, byerekana ko izo nkingo zitera ubudahangarwa bw'umubiri ^[4].

Kugirango babone uburyo bwo kuringaniza imbyaro no kuvura indwara ziterwa na hormone, abashakashatsi bakoze inkingo zigamije hCG. Immunogens yateguwe muguhuza peptide ya syntetique yerekana imiterere yibanze ya hCG β-subunit nabatwara proteine. Mugihe cyiterambere, abashakashatsi bashushanyije peptide nyinshi yuburebure butandukanye bwatoranijwe mu karere ka C-terminal ka β subunit kandi bagerageza ubushobozi bwabo bwo gutera antibodiyide zishobora guhangana na hCG no guhagarika ibikorwa byayo muri vivo. Ubwanyuma, igice cya 37-amino acide peptide igereranya akarere ka C-terminal ya un subunit yatoranijwe nka antigen yinkingo. Diphtheria toxoid yatoranijwe nkuwitwaye, biganisha ku gutegura urukingo rwa mbere rwa prototype. Nyuma y’ibigeragezo ku moko menshi, uru rukingo rwateye antibody nyinshi kurwanya hCG, kandi igabanuka ry’uburumbuke ryagaragaye ku bana bakingiwe. Ibi byatanze ubumenyi bushya hamwe nuburyo bushoboka bwo kurwanya uburumbuke bwabantu no kuvura indwara bifitanye isano ^[5].

Umwanzuro

Nka misemburo ya glycoproteine, hCG igira uruhare runini muri sisitemu yimyororokere: ku bagore, ikora ihuza imisemburo ya luteinizing / chorionic gonadotropin reseptor (LHCGR) kugira ngo ikomeze gutwita hakiri kare (gushyigikira imisemburo ya progesterone no gutera intanga zihamye), gutera imbere kwa oocyte no gutera intanga ngabo. Ku bagabo, itera selile ya Leydig guhuza no gusohora testosterone, ifasha iterambere ryimyororokere na spermatogenez.

Ibyerekeye Umwanditsi

Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.

Umwanditsi w'ikinyamakuru

Nwabuobi C numushakashatsi wibanze kubijyanye nubushakashatsi bwa chorionic gonadotropin (hCG). Ubushakashatsi bwabo bwibanze cyane cyane ku gusesengura imikorere y’ibinyabuzima ya hCG no gucukumbura isano bifitanye n’ubuvuzi. Nwabuobi C ikunze gukoresha uburyo bwubushakashatsi nkubuhanga bwibinyabuzima bwa molekuline hamwe nisesengura ryikitegererezo cyamavuriro kugirango ikore ubushakashatsi bwimbitse, igamije kurushaho gusobanukirwa nuburyo bwimikorere ya physiologiya ya hCG nagaciro kayo mubikorwa byubuvuzi. Nwabuobi C yanditse murutonde rwerekana [1].

Imirongo ijyanye

[1] Nwabuobi C, Arlier S, Schatz F, Guzeloglu-Kayisli O, Lockwood CJ, Kayisli UA. HCG: Imikorere yibinyabuzima hamwe nubuvuzi bukoreshwa. Ikinyamakuru mpuzamahanga cyubumenyi bwa molecular 2017; 18 (10) .DOI: 10.3390 / ijms18102037.

[2] Wang W, Ge L, Zhang LL, n'abandi. Mechanism ya chorionic gonadotropine yabantu muburyo bwo kwakira endometrale ikoresheje miR-126-3p / PI3K / Akt / eNOS axis. Ikinyamakuru Kaohsiung cyubumenyi bwubuvuzi 2023; 39(5): 468-477.DOI: 10.1002 / kjm2.12672.

[3] Moyle WR, Bahl OP, März L. Uruhare rwa karubone ya chorionic gonadotropine yumuntu muburyo bwo gukora imisemburo. Ikinyamakuru cya Chimie y’ibinyabuzima 1975; 250(23): 9163-9169.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=172504&query_hl=1.

[4] Talwar GP, Hingorani V, Kumar S, n'abandi. Icyiciro cya mbere cyamavuriro hamwe nuburyo butatu bwurukingo rwa chorionic gonadotropin. Kuringaniza imbyaro 1990; 41(3): 301-316.DOI: 10.1016 / 0010-7824 (90) 90071-3.

Stevens VC. Gukoresha peptide ya sintetike nka immunogène mugutezimbere urukingo rurwanya chorionic gonadotropine. Ciba Yabonye Ikimenyetso 1986; 119: 200-225.DOI: 10.1002 / 9780470513286.ch12.

INGINGO ZOSE N'AMAKURU Y’IBICURUZWA BITANZWE KURI URU RUBUGA BISANZWE KUBONA AMAKURU N'INTEGO Z'UBUREZI.  

Ibicuruzwa byatanzwe kururu rubuga bigenewe gusa mubushakashatsi bwa vitro. Mu bushakashatsi bwa vitro (Ikilatini: * mu kirahure *, bisobanura mu bikoresho by'ibirahure) bikorwa hanze y'umubiri w'umuntu. Ibicuruzwa ntabwo ari imiti, ntabwo byemejwe n’ikigo cy’Amerika gishinzwe ibiryo n’ibiyobyabwenge (FDA), kandi ntigomba gukoreshwa mu gukumira, kuvura, cyangwa gukiza indwara iyo ari yo yose y’ubuvuzi, indwara, cyangwa indwara. Birabujijwe rwose n'amategeko kwinjiza ibyo bicuruzwa mumubiri wabantu cyangwa inyamaswa muburyo ubwo aribwo bwose.