HCG 5000iu

▎ Ọdịdị HCG

▎ Nyocha HCG

Kedu ihe ndabere nyocha nke hCG?

hCG na-arụ ọrụ dị oke mkpa na ọgwụ ịmụ nwa, gụnyere ịnọgide na-atụrụ ime n'oge na ịkpata ovulation na teknụzụ ọmụmụ enyere aka. A na-ewepụta hCG eke sitere na mmamịrị ụmụ nwanyị dị ime, na-egosipụta oke n'inweta na mmesi obi ike. Nke a anaghị emezu ihe ndị chọrọ ụlọ ọgwụ na-eto eto, na-achọ ka mmepe nke hCG sịntetịt wee nye ebe ọgwụ kwụsiri ike na ntụkwasị obi.

Ọganihu dị na teknụzụ injinịa mkpụrụ ndụ ihe nketa enyerela cloning na ngosipụta nke mkpụrụ ndụ ihe nketa hCG, na-etinye ntọala teknụzụ maka nyocha hCG synthetic. Site n'iwebata mkpụrụ ndụ hCG n'ime sel ndị ọbịa dị ka yist ma ọ bụ mkpụrụ ndụ hamster ovary (CHO), enwere ike nweta okwu recombinant nke hCG. HCG sịntetịt na-enye ihe atụ dị ọcha maka ọmụmụ ihe nhazi na arụ ọrụ, na-eme ka nghọta miri emi banyere usoro ọrụ ya na-enyere aka ịmepụta usoro ọgwụgwọ dị irè karị. Na mgbakwunye, enwere ike iji hCG sịntetik mee ihe iji dozie reagents diagnostic, na-eme ka izi ezi nke nyocha afọ ime yana nyochaa ọnọdụ ndị metụtara ya.

Gịnị bụ usoro nke ime ihe maka hCG?

Usoro n'ịkwagide afọ ime: hCG bụ isi na-emepụta site na mkpụrụ ndụ syncytiotrophoblast dị iche iche ma na-eje ozi dị ka mgbama embrayo dị mkpa dị mkpa maka ịkwado afọ ime. Ọ na-arụ ọrụ cascades ọtụtụ akara ngosi site na ijikọ na hormone luteinizing / mmadụ chorionic gonadotropin receptor (LHCGR). Nchoputa nke Nwabuobi C na-egosi na site na mmekorita ya na ihe ngbanwe uto beta receptor (TGFβR) na-eme ka uzo nne di ka Smad2, protein kinase C (PKC), na/ma obu protein kinase A (PKA). N'ịkwalite uterine endothelial angiogenesis, hCG na-enyere aka inye nri zuru oke na oxygen maka mmepe embrayo; Na-edobe uterine myometrial quiescence, ọ na-emepụta a kwụsiri ike intrauterine gburugburu ebe obibi na-ekwe ka embrayo ịkụnye na mmepe; N'ịkwalite usoro mgbochi ọrịa na interface nke nne-nwa ebu n'afọ, hCG na-agbanwe usoro ahụ ji alụso ọrịa ọgụ nke nne iji gbochie ịjụ embrayo, na-eme ka ọganihu ime ime na-aga nke ọma ^[1].

Usoro nke ime ihe n'ịkwalite nnabata endometrial: Nnwale na vivo site na iji ụdị nhụsianya embrayo implantation (EID) na mkpụrụ ndụ epithelial endometrial mmadụ (EECs) kpughere na hCG na-eme ka nnabata endometrial dị na ụmụ oke EID. hCG na-ahazi axis miR-126-3p/PIK3R2 site na ịkwalite okwu miR-126-3p na igbochi PIK3R2, ebe miR-126-3p na-achọ PIK3R2. A kwalitere mmụba EEC ka ọgwụgwọ hCG gasịrị mana egbochiri ya mgbe mbelatara miR-126-3p. Nnwale na vivo na in vitro gosipụtara na hCG na-eme ka nnabata endometrial na-eme ka embrayo tinye ya n'ọrụ site na ịgbalite ụzọ PI3K/Akt/eNOS site na axis miR-126-3p/PIK3R2 ^[2].

Ọgụgụ 1 hCG kwalitere nnabata endometrial na ụmụ oke EID ^[2].

Mmetụta nke glycosylation na usoro hormonal: Nnyocha na akụkụ glycosylated nke hCG na-egosi na iwepụ ihe dị iche iche nke shuga sitere na ihe ndị na-emepụta hCG na-emetụta ike ha jikọtara ọnụ na mkpụrụ ndụ Leydig na ike ha na-akpali testosterone na cyclic AMP (cAMP) njikọ. Site n'usoro mwepụ nke sialic acid, galactose, N-acetylglucosamine na mannose foduru, ọgwụ homonụ dị irè chọrọ iji kpalite mmepụta steroid ji nwayọọ nwayọọ na-abawanye, ebe ikike ịkpali mkpokọta cAMP belatara nke ukwuu. Mkpụrụ ọgwụ glycosidase a na-agwọ dị ala gosipụtara mmetụta mgbakwunye mgbe a na-enyocha ya na hCG maka ikike ha ịkpalite njikọ testosterone. Otú ọ dị, usoro ndị a bụ ndị na-egbochi ike nke hCG na-ebute mkpokọta cAMP, na-atụ aro na mwepụ nke glycosylation na-emetụta obere njikọ nke hormone-cell ka ọ na-ebelata ikike nke homonụ agbụ iji mee ka adenylate cyclase rụọ ọrụ. Nke a na-emesi ike ọrụ dị oke mkpa nke glycosylation na ntụgharị mgbaàmà n'ime usoro hCG nke omume ^[3]..

Kedu ihe bụ ngwa nke hCG?

Nchọpụta ime ime mmalite: A na-eji hCG sịntetik eme ihe iji kwadebe reagents nchọpụta. Na omume ụlọ ọgwụ, a na-ekpebi ime ime site na ịlele ọkwa hCG na mmamịrị ma ọ bụ ọbara nwanyị. Ihe dị ka ụbọchị 6-7 mgbe njikọta spam nwoke na nwanyị gachara, mkpụrụ ndụ trophoblast na-amalite izopụta hCG. Ka afọ ime na-aga n'ihu, ọkwa hCG n'ime ọbara na mmamịrị na-ebili ngwa ngwa. Iji hCG sịntetik dị ka ọkọlọtọ na-enye ohere maka nguzobe ziri ezi nke ọkọlọtọ ọkọlọtọ maka reagents nchọpụta, na-eme ka ọnụọgụ nke ọdịnaya hCG ziri ezi na nlele. Dịka ọmụmaatụ, mpempe ule ime ime na-adabere na teknụzụ immunochromatography, ebe ọgwụ mgbochi ndị a kwadebere site na hCG synthetic kpọmkwem na-ejikọta hCG na mmamịrị, yana mmeghachi omume agba na-egosi ọnọdụ ime. Dị ka ọ na-adịkarị, mgbe oge furu efu, ụmụ nwanyị nwere ike iji ụzọ ndị dị otú ahụ maka nyocha afọ ime nke mbụ, na-enye ihe ndabere maka nlekọta na nlekọta nwa oge na-esote ^[1].

Nyochaa ọnọdụ metụtara ime ime: N'oge ime ime, nsochi mgbanwe dị ike na ọkwa hCG nne nne na-enyere aka n'ịchọpụta na nyochaa nsogbu dị iche iche metụtara ime. Dịka ọmụmaatụ, n'ime afọ ime ectopic, ebe akwa fatịlaịza na-etinye n'èzí oghere uterine, mkpụrụ ndụ trophoblastic na-etolite na-adịghị mma. Nke a na-ebute ihe nzuzo hCG dị ala ma e jiri ya tụnyere ime ime intrauterine nkịtị na ogologo oge okpukpu abụọ. Nyochaa na-aga n'ihu na ọkwa hCG ọbara, yana nyocha nke ultrasound, na-eme ka nchọpụta mmalite nke ime ectopic dị mfe. Nke a na-enye ohere maka ntinye aka na ọgwụgwọ oge, na-egbochi nsogbu siri ike. Na mgbakwunye, maka ọrịa trophoblastic dị ka hydatidiform mole, ọkwa hCG na-egosikarị elu dị njọ. Mgbe ọgwụgwọ gasịrị, nlekota hCG na-aga n'ihu nwere ike nyochaa ịdị irè ọgwụgwọ ma chọpụta nlọghachi azụ. Ọkwa hCG na-ebuli ma ọ bụ na-arị elu na-egosi ọrịa nwere ike ịpụta ma ọ bụ nlọghachi azụ, na-achọkwu nyocha na ntinye aka ^[1].

Kedu ihe bụ ọganihu nnwale gbasara hCG?

Ọgwụ mgbochi mgbochi mepụtara site na iji β-subunit nke hCG zuru oke dị ka immunogen na akụkụ 37-amino acid peptide (C-terminus 109-145) ka immunogen agafeela nsi na nchekwa preclinical ma mechaa ule ụlọ ọgwụ Phase I na Phase II. N'ime nnwale ụlọ ọgwụ atụnyere nke mbụ nke metụtara ụmụ nwanyị 116 ndị ọrụ afọ ofufo bụ ndị megoro tubal ligation, a nwalere usoro ọgwụ mgbochi β-hCG atọ. Nsonaazụ gosiri na ụmụ nwanyị niile gbara ọgwụ mgbochi mepụtara ọgwụ mgbochi megide hCG na tetanus, na-egosi ọgwụ mgbochi ndị a na-akpali mmeghachi omume mgbochi mmadụ nke ọma ^[4]..

Iji nweta akara ọmụmụ na ịgwọ ọrịa ndị metụtara hormone, ndị nchọpụta mepụtara ọgwụ mgbochi na-ezubere hCG. A kwadebere immunogens site na ijikọta peptides sịntetik na-anọchite anya nhazi mbụ nke hCG β-subunit na ndị na-ebu protein. N'oge mmepe, ndị na-eme nchọpụta na-emepụta ọtụtụ peptides nke ogologo dịgasị iche iche nke a na-ahọrọ site na mpaghara C-terminal nke β subunit ma nwalee ike ha nwere ike ịmepụta ọgwụ nje nwere ike imeghachi omume na hCG ma wepụ ọrụ ya na vivo. N'ikpeazụ, a na-ahọrọ akụkụ peptide 37-amino acid na-anọchite anya mpaghara C-terminal nke β subunit dị ka antigen ọgwụ mgbochi. A họọrọ toxoid diphtheria dị ka onye na-ebu, na-eduga na nkwadebe nke ọgwụ mgbochi mbụ. Na-esochi ule n'ofe ụdị dị iche iche, ọgwụ mgbochi a welitere ọkwa mgbochi mmadụ megide hCG nke ọma, yana mbelata nke ọma ọmụmụ na adaka gbachiri ọgwụ. Nke a nyere nghọta ọhụrụ na ụzọ ndị nwere ike isi nweta njikwa ọmụmụ mmadụ na ọgwụgwọ ọrịa metụtara ya ^[5].

Mmechi

Dị ka isi hormone glycoprotein, hCG na-arụ ọrụ nchịkwa etiti na usoro ọmụmụ: n'ime ụmụ nwanyị, ọ na-arụ ọrụ site na ijikọta hormone luteinizing / chorionic gonadotropin receptor (LHCGR) iji kwado ime ime n'oge (na-akwado luteal progesterone secretion na ịmepụta gburugburu uterine kwụsiri ike), na-akwalite oocyte maturation na enhavulation endometrium na endometrium. ntinye. N'ime ụmụ nwoke, ọ na-akpali mkpụrụ ndụ Leydig ka ha mepụta na nzuzo testosterone, na-enyere aka mmepe akụkụ ọmụmụ na spermatogenesis.

Banyere Onye dere ya

Ihe niile a kpọtụrụ aha n'elu bụ Cocer Peptides na-enyocha, dezie ma chịkọta ya.

Onye ode akwụkwọ akụkọ sayensị

Nwabuobi C bụ onye nyocha na-elekwasị anya na ngalaba ọmụmụ chorionic gonadotropin (hCG). Nnyocha ha gbadoro ụkwụ na nyocha ọrụ ndu nke hCG na inyocha njikọ ya na ngwa ụlọ ọgwụ. Nwabuobi C na-anabatakarị usoro nnwale dị ka usoro ihe ọmụmụ ihe omimi na nyocha nyocha ụlọ ọgwụ iji mee nyocha miri emi, na-achọ ime ka nghọta nke usoro physiological nke hCG dị omimi na uru bara uru na ọnọdụ ụlọ ọgwụ. Nwabuobi C ka edepụtara n'akwụkwọ ntụaka [1].

▎ Okwu ndị dị mkpa

[1] Nwabuobi C, Arlier S, Schatz F, Guzeloglu-Kayisli O, Lockwood CJ, Kayisli UA. hCG: Ọrụ ndu na ngwa ụlọ ọgwụ. Akwụkwọ akụkọ International nke Science Molecular 2017; 18 (10).DOI: 10.3390/ijms18102037.

[2] Wang W, Ge L, Zhang LL, et al. Usoro nke gonadotropin chorionic mmadụ na nnabata endometrial site na axis miR-126-3p/PI3K/Akt/eNOS. Akwụkwọ akụkọ Kaohsiung nke sayensị ahụike 2023; 39(5): 468-477.DOI: 10.1002/kjm2.12672.

[3] Moyle WR, Bahl OP, März L. Ọrụ nke carbohydrate nke gonadotropin chorionic mmadụ na usoro nke homonụ. Akwụkwọ akụkọ nke Chemistry Biological 1975; 250(23): 9163-9169 .

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=172504&query_hl=1.

[4] Talwar GP, Hingorani V, Kumar S, et al. Nnwale ụlọ ọgwụ nke agba nke mbụ nwere usoro atọ nke ọgwụ mgbochi chorionic gonadotropin na-emegide mmadụ. Mgbochi ime afọ 1990; 41(3): 301-316.DOI: 10.1016/0010-7824 (90)90071-3.

[5] Stevens VC. Iji peptides sịntetik dị ka immunogens maka ịmepụta ọgwụ mgbochi megide gonadotropin chorionic mmadụ. Ciba chọtara Symp 1986; 119: 200-225.DOI: 10.1002/9780470513286.ch12.

Edemede na ozi ngwaahịa niile enyere na webụsaịtị a bụ naanị maka mgbasa ozi na ebumnuche mmụta.  

Ngwaahịa ndị enyere na webụsaịtị a bụ naanị maka nyocha in vitro. A na-eme nyocha in vitro (Latin: * na iko *, nke pụtara na ihe eji enyo) na mpụga ahụ mmadụ. Ngwaahịa ndị a abụghị ọgwụ, ndị US Food and Drug Administration (FDA) akwadobeghị ya, ha agaghịkwa eji ya gbochie, gwọọ, ma ọ bụ gwọọ ọnọdụ ahụike ọ bụla, ọrịa, ma ọ bụ ọrịa. Iwu amachibidoro nke ọma iwebata ngwaahịa ndị a n'ime ahụ mmadụ ma ọ bụ anụmanụ n'ụdị ọ bụla.