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1 pahu (10 Vials)
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▎ Hana HCG
Puna: PubChem |
IUPAC Condensed: N(1)Cys-Gly-OH.H-Aad(1)-OH Huihui Molekala: C 11H 19N 3O 6S Kaumaha Molekala:321.35g/mol Helu CAS: 9002-61-3 PubChem CID: 4369448 Synonyms: Chorionic gonadotrophin；CHEMBL1233255 |
▎ ʻImi HCG
He aha ke kumu noiʻi o ka hCG?
He kuleana koʻikoʻi ka hCG i ka lāʻau lapaʻau hānau, me ka mālama ʻana i ka hāpai mua ʻana a me ka hoʻoulu ʻana i ka ovulation i nā ʻenehana reproductive kōkua. Hoʻopuka mua ʻia ka hCG maoli mai ka mimi wahine hāpai, e hōʻike ana i nā palena o ka lako a me ka hōʻoia maʻemaʻe. ʻAʻole hiki i kēia ke hoʻokō i nā koi lapaʻau e ulu nei, pono e hoʻomohala i ka hCG synthetic e hāʻawi i kahi kumu lāʻau paʻa a hilinaʻi.
ʻO ka holomua ʻana i ka ʻenehana ʻenekinia i hiki ai ke cloning a me ka hōʻike ʻana o ka gene hCG, e kau ana i ke kumu ʻenehana no ka noiʻi hCG synthetic. Ma ka hoʻokomo ʻana i ka gene hCG i loko o nā pūnaewele hoʻokipa e like me ka hū a i ʻole nā pūnaewele hamster ovary (CHO), hiki ke hoʻokō ʻia ka hōʻike recombinant o ka hCG. Hāʻawi ka Synthetic hCG i nā laʻana maʻemaʻe no nā haʻawina hoʻolālā a me ka hana, e hoʻomaʻamaʻa i ka ʻike hohonu o kāna mau hana hana a hiki i ka hoʻomohala ʻana i nā ala lapaʻau ʻoi aku ka maikaʻi. Eia hou, hiki ke hoʻohana ʻia ka hCG synthetic e hoʻomākaukau i nā reagents diagnostic, e hoʻonui i ka pololei o ka maʻi ʻōpū a me ka nānā ʻana i nā kūlana pili.
He aha ke ʻano o ka hana no ka hCG?
Mechanism i ka mālama ʻana i ka hāpai ʻana: ua hana mua ʻia ka hCG e nā cell syncytiotrophoblast ʻokoʻa a lawelawe ʻo ia ma ke ʻano he hōʻailona embryonic koʻikoʻi no ka mālama ʻana i ka hāpai ʻana. Hoʻoulu ia i nā cascades hōʻailona he nui ma o ka hoʻopaʻa ʻana i ka luteinizing hormone/human chorionic gonadotropin receptor (LHCGR). Hōʻike ʻia ka noiʻi a Nwabuobi C ma o ka launa pū ʻana a i ʻole ka hoʻololi ʻana me ka mea hoʻololi i ka ulu ʻana beta receptor (TGFβR), hoʻāla ia i nā ala hōʻailona makuahine e like me Smad2, protein kinase C (PKC), a/a i ʻole protein kinase A (PKA). I ka hoʻouluʻana i ka angiogenesis endothelial uterine, kōkua ka hCG i ka hāʻawiʻana i ka meaʻai kūpono a me ka oxygen no ka uluʻana o ka embryonic; Ma ka mālama ʻana i ka quiescence myometrial uterine, hana ia i kahi ʻano intrauterine paʻa e kūpono i ka embryo implantation a me ka ulu ʻana; I ka hoʻoikaika ʻana i ka hoʻoponopono ʻana i ka pale ʻana i ka maternal-fetal interface, hoʻololi ʻo hCG i ka ʻōnaehana immune maternal e pale ai i ka hōʻole ʻana i ka embryo, e hōʻoia i ka holomua o ka hāpai ʻana [1].
Mechanism of action in improvement endometrial receptivity: In vivo experiments using a embryo implantation dysfunction (EID) mouse model and human endometrial epithelial cell (EECs) i hōʻike i ka hCG hoʻonui i ka endometrial receptivity i nā ʻiole EID. Hoʻoponopono ka hCG i ka axis miR-126-3p/PIK3R2 ma o ka hoʻolaha ʻana i ka hōʻike miR-126-3p a me ka pale ʻana i ka PIK3R2, aʻo ka miR-126-3p e kuhikuhi ana i ka PIK3R2. Ua hoʻonui ʻia ka hoʻonui ʻana o EEC ma hope o ka mālama ʻana i ka hCG akā ua kāohi ʻia i ka wā i hoʻohaʻahaʻa ʻia ka miR-126-3p. Ua hōʻoia ʻia nā hoʻokolohua in vivo a me in vitro e hoʻonui ka hCG i ka loaʻa ʻana o ka endometrial a hoʻomaʻamaʻa i ka embryo implantation ma ka hoʻoulu ʻana i ke ala PI3K/Akt/eNOS ma o ka miR-126-3p/PIK3R2 axis [2].
Hoʻonui ka helu 1 hCG i ka loaʻa ʻana o ka endometrial i nā ʻiole EID [2].
Ka hopena o ka glycosylation ma nā hana hormonal: Hōʻike nā haʻawina e pili ana i ka glycosylated moiety o hCG i ka wehe ʻana i nā koena kō like ʻole mai nā derivatives hCG e hoʻopilikia ʻokoʻa i ko lākou hiki ke hoʻopaʻa i nā cell Leydig a me ko lākou hiki ke hoʻoulu i ka testosterone a me ka cyclic AMP (cAMP) synthesis. Me ka wehe ʻana i ka waikona sialic, galactose, N-acetylglucosamine, a me nā koena mannose, ua hoʻonui nui ʻia ka nui o ka hormone pono e hoʻoulu ai i ka hana steroid, aʻo ka hiki ke hoʻoulu i ka hōʻiliʻili cAMP ua emi nui. Ua hōʻike ʻia nā derivatives hormone haʻahaʻa haʻahaʻa glycosidase i nā hopena hoʻohui ke nānā ʻia me ka hCG no ko lākou hiki ke hoʻoulu i ka synthesis testosterone. Eia nō naʻe, ʻo kēia mau derivatives he mau mea hoʻopaneʻe ikaika o ka hCG-induced cAMP accumulation, e hōʻike ana i ka wehe ʻana o ka glycosylation e hoʻopili liʻiliʻi i ka hoʻopaʻa ʻana i ka hormone-cell oiai e hoʻemi ana i ka hiki o nā hormones paʻa e hoʻāla i ka adenylate cyclase. Hōʻike hou kēia i ka hana koʻikoʻi o ka glycosylation i ka transduction hōʻailona i loko o ka hana hCG o ka hana [3].
He aha nā hoʻohana o ka hCG?
Ka hōʻoia ʻana o ka hāpai mua ʻana: Hoʻohana ʻia ka hCG synthetic no ka hoʻomākaukau ʻana i nā reagents ʻike. Ma ka hana lapaʻau, hoʻoholo ʻia ka hāpai ʻana ma ke ana ʻana i nā pae hCG i loko o ka mimi a i ʻole ke koko o ka wahine. Ma kahi o 6-7 mau lā ma hope o ka fertilization, hoʻomaka ka huna ʻana o nā cell trophoblast i ka hCG. I ka holomua ʻana o ka hānau ʻana, piʻi wikiwiki nā pae hCG i ke koko a me ka mimi. ʻO ka hoʻohana ʻana i ka hCG synthetic ma ke ʻano he maʻamau e hiki ai i ka hoʻokumu pololei ʻana i kahi pihi maʻamau no nā reagents ʻike, hiki i ka helu pono ʻana o ka ʻike hCG i nā laʻana. No ka laʻana, hilinaʻi nā ʻāpana hoʻāʻo maʻamau i ka ʻenehana immunochromatography, kahi e hoʻopaʻa pono ai nā antibodies mai ka hCG synthetic i ka hCG i loko o ka mimi, me kahi ʻano kala e hōʻike ana i ke kūlana hāpai. ʻO ka maʻamau, ma hope o ka hala ʻana o ka manawa, hiki i nā wahine ke hoʻohana i ia ʻano hana no ka loiloi mua ʻana o ka hāpai ʻana, e hāʻawi ana i kumu no ka mālama a me ka hoʻokele prenatal [1].
Ka nānā ʻana i nā kūlana pili i ka hāpai ʻana: I ka wā hāpai, ʻo ka ʻimi ʻana i nā loli ikaika i nā pae hCG makuahine e kōkua i ka ʻike ʻana a me ka nānā ʻana i nā maʻi pili i ka hāpai ʻana. No ka laʻana, i ka hāpai ʻana i ka ectopic, kahi i hoʻokomo ʻia ai ka hua manu i hoʻomoʻa ʻia ma waho o ka ʻōpū o ka ʻōpū, ulu ʻole nā pūnaewele trophoblastic. Loaʻa kēia i ka haʻahaʻa haʻahaʻa hCG i hoʻohālikelike ʻia i nā hāpai intrauterine maʻamau a me kahi manawa pālua lōʻihi. ʻO ka nānā mau ʻana i nā pae hCG koko, i hui pū ʻia me nā hoʻokolohua ultrasound, e hoʻomaʻamaʻa i ka ʻike mua ʻana o nā hāpai ectopic. Hāʻawi kēia i ka manawa kūpono a me ka mālama ʻana, e pale ana i nā pilikia koʻikoʻi. Eia kekahi, no nā maʻi trophoblastic e like me ka mole hydatidiform, hōʻike pinepine nā pae hCG i ka piʻi ʻana. Ma hope o ka mālama ʻana, hiki i ka nānā ʻana i ka hCG ke nānā i ka pono therapeutic a ʻike i ka hoʻi hou ʻana. ʻO ka hoʻokiʻekiʻe mau ʻana a i ʻole ka piʻi ʻana o nā pae hCG e hōʻike ana i ke koena o ka maʻi a i ʻole ka hoʻihoʻi hou ʻana, pono e noiʻi hou aʻe [1].
He aha nā holomua hoʻokolohua e pili ana i ka hCG?
Hoʻokumu ʻia nā lāʻau lapaʻau contraceptive me ka hoʻohana ʻana i ka β-subunit holoʻokoʻa o ka hCG ma ke ʻano he immunogen a me kahi ʻāpana peptide 37-amino acid (C-terminus 109-145) ʻoiai ua hala ka immunogen i nā hoʻokolohua preclinical a me ka palekana a ua hoʻopau i nā hoʻokolohua lapaʻau Phase I a me Phase II. Ma kahi hoʻokolohua hoʻokolohua hoʻohālikelike Phase I e pili ana i nā wahine manawaleʻa wahine 116 i hoʻopaʻa ʻia i ka ligation tubal, ua hoʻāʻo ʻia ʻekolu mau hana kano i hoʻokumu ʻia i ka β-hCG. Ua hōʻike nā hopena i nā wahine āpau i hoʻomohala i nā antibodies e kūʻē i ka hCG a me ka tetanus, e hōʻike ana i kēia mau lāʻau lapaʻau e hoʻoulu maikaʻi i ka pane kūlohelohe kanaka [4].
No ka hoʻokō ʻana i ka mana hānau a mālama i nā maʻi pili i ka hormone, ua hoʻomohala nā mea noiʻi i nā lāʻau lapaʻau e kuhikuhi ana i ka hCG. Ua hoʻomākaukau ʻia nā immunogens ma ka hoʻopili ʻana i nā peptides synthetic e hōʻike ana i ke ʻano kumu maoli o ka hCG β-subunit i nā mea lawe pūmua. I ka wā o ka hoʻomohala ʻana, ua hana nā mea noiʻi i nā peptide he nui o nā lōʻihi like ʻole i koho ʻia mai ka ʻāpana C-terminal o ka subunit β a hoʻāʻo i ko lākou hiki ke hoʻoulu i nā antibodies hiki ke pane me ka hCG a hoʻokaʻawale i kāna hana i loko o vivo. ʻO ka hope loa, ua koho ʻia kahi ʻāpana peptide 37-amino acid e hōʻike ana i ka ʻāpana C-terminal o ka subunit β ma ke ʻano he antigen kano. Ua koho ʻia ʻo Diphtheria toxoid ma ke ʻano he mea lawe, e alakaʻi ana i ka hoʻomākaukau ʻana i ka lāʻau lapaʻau prototype mua. Ma hope o nā hoʻāʻo ʻana ma waena o nā ʻano mea like ʻole, ua hoʻoulu maikaʻi kēia kano i nā pae antibody koʻikoʻi e kūʻē i ka hCG, a ua ʻike ʻia ka emi ʻana o ka momona i nā baboons i hoʻopaʻa ʻia. Hāʻawi kēia i nā ʻike hou a me nā ala kūpono no ka mālama ʻana i ka momona o ke kanaka a me ka mālama ʻana i nā maʻi pili [5].
Ka hopena
Ma ke ʻano he hormone glycoprotein kī nui, hoʻokani ka hCG i ke kuleana koʻikoʻi i ka ʻōnaehana hānau: i nā wahine, hana ia ma ka hoʻopaʻa ʻana i ka hormone luteinizing / chorionic gonadotropin receptor (LHCGR) e hoʻomau i ka hāpai mua ʻana (kākoʻo i ka huna ʻana o ka progesterone luteal a me ka hoʻokumu ʻana i kahi kaiapuni uterine paʻa) implantation. I nā kāne, hoʻoulu ia i nā pūnaewele Leydig e synthesize a huna i ka testosterone, kōkua i ka hoʻomohala ʻana o ka ʻōpū hānau a me ka spermatogenesis.
No ka mea kakau
ʻO nā mea i ʻōlelo ʻia ma luna nei ua noiʻi ʻia, hoʻoponopono ʻia a hōʻuluʻulu ʻia e Cocer Peptides.
Mea kākau moʻolelo ʻepekema
He kanaka noiʻi ʻo Nwabuobi C e kālele ana i ke kahua o nā haʻawina kanaka chorionic gonadotropin (hCG). Hoʻokumu nui kā lākou noiʻi i ka nānā ʻana i nā hana olaola o ka hCG a me ka ʻimi ʻana i kāna mau pilina me nā noi lapaʻau. Hoʻohana pinepine ʻo Nwabuobi C i ka hui pū ʻana o nā ʻano hoʻokolohua e like me ka molecular biology techniques a me ka hoʻāʻo ʻana i ka lāʻau lapaʻau e hana i ka noiʻi hohonu, me ka manaʻo e hoʻonui i ka hoʻomaopopo ʻana i nā mīkini physiological o ka hCG a me kāna waiwai kūpono i nā hiʻohiʻona lapaʻau. Ua helu ʻia ʻo Nwabuobi C ma ka ʻōlelo kuhikuhi [1].
