▎ Livagen Binciken
Menene asalin bincike na Livagen?
The Free Radical Theory of Aging: A cikin 1950s, Harman ya ba da shawarar ka'idar tsufa na kyauta, yana ba da shawarar cewa radicals kyauta da aka samar yayin metabolism na salula zai kai hari ga kwayoyin halitta, wanda zai haifar da lalacewar tantanin halitta da tsufa. Wannan ka'idar ta ba da tushe na ka'idar don binciken abubuwan hana tsufa kuma ya sa masana kimiyya su nemo abubuwan da za su iya lalata radicals kyauta ko haɓaka ƙarfin antioxidant na sel.
Gano Abubuwan Peptide: Tare da ci gaba da zurfin nazarin ilimin halittu, masana kimiyya sun gano cewa abubuwan peptide suna taka muhimmiyar rawa a cikin siginar tantanin halitta, tsarin rayuwa, da sauran fannoni. Wasu abubuwan peptide suna da ayyuka irin su antioxidant, anti-inflammatory, da inganta gyaran sel, wanda ke ba da sabon jagora don haɓaka sabbin magungunan rigakafin tsufa.
Bincike da Ci gaban Livagen: Dangane da wannan baya, masana kimiyya na Rasha sun haɓaka Livagen bisa bincikensu akan abubuwan peptide. Sun fitar da gutsutsayen peptide na bioactive daga kyallen dabbobi kuma a ƙarshe sun sami Livagen bayan jerin gwaje-gwaje da ingantawa. Nazarin ya nuna cewa Livagen na iya daidaita tsarin tafiyar da rayuwa na sel da haɓaka ƙarfin antioxidant na sel, ta haka ne ke jinkirta tsufar salula.
Menene tsarin aikin Livagen?
1. Tasiri kan Tsarin narkewar abinci
Gudanar da Ayyukan Enzymes Digestive: Nazarin ya nuna cewa Livagen (Lys-Glu-Asp-Ala) peptide ne mai rauni mai rauni. The peptide hydrolases a cikin ƙananan hanji ba zai iya yin hydrolyze Livagen zuwa ɗan ƙaramin ƙarfi ba [1] . A ƙarƙashin yanayin in vitro, Livagen na iya rage ayyukan glycyl-L-leucine dipeptidase a cikin ƙananan hanji da kashi 50%. Bayan da aka ba da Livagen ta baki ga berayen na tsawon makonni biyu, ayyukan enzymes masu narkewa a cikin kananan dabbobi sun ragu, yayin da a cikin tsoffin dabbobi suka karu. Yana da mahimmanci a ambaci cewa a mafi yawan lokuta, aikin enzyme na tsohuwar berayen bayan karɓar Livagen ya kasance kusa da matakin dabbobin matasa a cikin rukunin kulawa [1].
2. Tasiri akan Enkephalin Degradeing Enzymes a cikin Serum
Hana Enkephalin Degrading Enzymes: Sakamakon sababbin bioregulators na peptide Livagen da Epitalon (Ala-Glu-Asp-Gly) akan tsarin tsarin opioid na endogenous an yi nazarin su, musamman ikon su na canza ayyukan enkephalin na lalata enzymes a cikin jini da yin hulɗa tare da masu karɓar opioid a cikin ɓangaren membrane na ƙwayoyin kwakwalwa. An ƙaddara aikin enkephalinase a cikin vitro ta hanyar auna ƙimar hydrolysis na ⊃3; H-Leu-enkephalin a gaban gwajin peptides. Livagen da Epitalon sun hana enkephalin lalata enzymes a cikin jinin ɗan adam. Idan aka kwatanta da sanannun masu hana peptidase irin su puromycin, leupeptin, da D-PAM, an tabbatar da Livagen ya fi tasiri. An ƙirƙira madaidaicin tasirin hanawa na Livagen da Epitalon, kuma ƙimar su IC₅₀ sun kasance 20 da 500 μM bi da bi [2, 3].
3. Tasirin Kariya akan Hanta
A matsayin wakili mai yuwuwar Hepatoprotective: Yin amfani da magunguna da yawa a cikin tsofaffi galibi shine dalilin rashin aikin hanta. Saboda haka, tare da karuwar shekaru, haɗarin hanta fibrotic induration, m da na kullum hepatitis karuwa. Wannan shine dalilin neman sababbin, tasiri, kuma marasa lahani na hanta. Wasu nazarin sun nuna cewa hadaddun polypeptide na hanta (Ventvil) da KEDA tetrapeptide (Lys-Glu-Asp-Ala, Livagen) suna da kaddarorin hanta, immunoprotective, da rigakafin tsufa. A cikin dabba da in vitro samfurin gwaji na hanta (hanta fibrotic induration, m da na kullum hepatitis), Ventvil da KEDA peptide sun nuna babban inganci. Ventvil da KEDA peptide suna da daidaiton tasiri - daidaita yanayin rigakafi da matsayin antioxidant da dawo da aikin hanta yayin hanta. Nazarin ya nuna cewa yayin aiwatar da tsufa, an tabbatar da matsakaicin tasirin hepatoprotective da immunoprotective na peptides [4].
4. Tasiri akan Chromatin na Lymphocytes
Kunna Chromatin na Lymphocytes: Sakamakon peptide na roba na Livagen akan ayyukan ƙwayoyin ribosomal, sigogi na denaturation na heterochromatin, polymorphism na tsarin C heterochromatin, da bambancin facultative heterochromatin a cikin lymphocytes na tsofaffi an yi nazari. Livagen ya haifar da kunna kwayoyin ribosomal, da depolymerization na heterochromatin na halitta a kusa da centromere, da kuma sakin kwayoyin halittar da aka hana saboda ƙaddamar da yankunan euchromatin da suka shafi shekaru a cikin chromosomes. Sakamakon ya nuna cewa Livagen ya haifar da deheterochromatization (kunnawa) na chromatin a cikin chromosomes na tsofaffi, wanda aka samu ta hanyar gyara heterochromatin da yankunan heterochromatinized a cikin chromosomes [5]..
Menene aikace-aikacen Livagen?
Bincike akan tsufa na salula
Livagen na iya shafar ayyukan mahimman enzymes waɗanda ke cikin gyaran tantanin halitta ko hanyoyin amsa damuwa. Alal misali, yana hulɗa tare da masu kula da epigenetic kuma yana rinjayar canje-canje a cikin tsarin chromatin da tsarin maganganun kwayoyin halitta, don haka yana daidaita tsarin tsufa na salula. Lalacewar Oxidative yana taruwa akan lokaci kuma yana haifar da rashin aiki na tsufa da ke da alaƙa da salon salula. Tasirin Livagen akan hanyar damuwa na oxidative yana daya daga cikin wuraren bincike, kuma yana iya jinkirta tsufa ta salula ta hanyar daidaita hanyoyin da suka dace.
Tasirin Cutar Hanta
Tare da karuwar shekaru, yin amfani da kwayoyi da yawa ta hanyar tsofaffi yakan haifar da rashin aiki na hanta, don haka ƙara haɗarin hanta fibrosis, m da kuma na kullum hepatitis. A wannan yanayin, yana da mahimmanci don nemo sabbin, masu tasiri, da marasa lahani masu hana hanta. Wasu nazarin sun nuna cewa hadadden polypeptide na hanta (Ventvil) da KEDA tetrapeptide (Lys-Glu-Asp-Ala, wato Livagen) suna da kaddarorin hanta, immunoprotective, da anti-tsufa. A cikin nau'ikan gwaji na dabba na cututtukan hanta (fibrosis hanta, m da kuma na kullum hepatitis) da kuma in vitro gwaje-gwaje, Ventvil da KEDA peptide sun nuna babban inganci. Ventvil da KEDA peptide suna da tasiri mai tasiri, wanda zai iya daidaita yanayin rigakafi da antioxidant da mayar da aikin hanta a lokacin hanta. Haka kuma, yayin tsarin tsufa, tasirin hepatoprotective da immunoprotective na waɗannan peptides sun kai iyakarsu [4].
Tasiri kan Ma'aunin Halittu na Majinyatan Ciwon daji
An gano cewa genome na marasa lafiya da ciwon nono na ductal yana da girma da yawa na karyawar DNA guda ɗaya, da yawan rashin daidaituwa na chromosomal, da ƙara yawan ƙwayar chromatin. Yin amfani da oligopeptide bioregulator Livagen da cobalt ions a matsayin masu gyare-gyare yana da tasiri mai kariya a kan al'adun lymphocyte na marasa lafiya da ciwon nono na ductal, wanda zai iya daidaita duk sigogin da aka yi nazari. Wannan yana nuna cewa ta hanyar nazarin ƙwayoyin lymphocytes na marasa lafiya da ciwon nono na ductal, ana iya kimanta tasirin maganin maganin ciwon nono [6]..
Tasiri akan Lymphocytes na Marasa lafiya tare da Hypertrophic Cardiomyopathy da 'Yan uwansu
Sakamakon peptide bioregulator Livagen (Lys-Glu-Asp-Ala) da aka yi amfani da shi kadai kuma a hade tare da cobalt ions a kan ayyukan yankunan da ke tattare da kwayoyin halitta (NORs) da kuma yawan haɗin gwiwar chromosomes na acrocentric a cikin lymphocytes na marasa lafiya tare da hypertrophic cardiomyopathy da danginsu. Sakamakon ya nuna cewa haɗin gwiwar aikin Livagen da cobalt ions sun ƙara yawan adadin NOR tare da babban maki na 2 a cikin marasa lafiya da danginsu. Wadannan mahadi kuma suna da tasiri mai mahimmanci akan ayyukan haɗin gwiwar chromosomes na acrocentric, wanda aka bayyana a matsayin karuwa mai girma a cikin wannan ma'auni a cikin ƙungiyoyin binciken biyu.
A wannan yanayin, aikin Livagen da cobalt ions ya fi tasiri. Tunda ayyukan NORs da yawan haɗin gwiwar chromosomes na acrocentric sun dogara ne akan ingancin ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta ta Livagen da cobalt ions Livagen da cobalt ions suna da tasiri akan lymphocytes na marasa lafiya tare da hypertrophic cardiomyopathy da danginsu, yana haifar da decondensation na heterochromatinized chromosomes. Wannan yana iya zama yanayin sakin kwayoyin halittar da ba a kunna su ba yayin aikin damfara a cikin daidaikun rukunin binciken. Wadannan bayanan suna ba da sabon bayani game da tasirin kariya na Livagen da Livagen + cobalt ions akan lymphocytes na marasa lafiya tare da hypertrophic cardiomyopathy da danginsu kuma suna iya ba da gudummawa ga ci gaban hanyoyin magani [7].
Tasiri kan Enkephalin Degradeing Enzymes a cikin Serum
An yi nazarin tasirin sabon peptide bioregulators Livagen (Lys-Glu-Asp-Ala) da Epitalon (Ala-Glu-Asp-Gly) akan tsarin tsarin opioid na endogenous, musamman ikon su na canza ayyukan enkephalin masu lalata enzymes a cikin jini da yin hulɗa tare da masu karɓar opioid a cikin ɓangaren membrane na ƙwayoyin kwakwalwa. Gwaje-gwajen in vitro sun ƙayyade ayyukan enkephalinase ta hanyar auna ƙimar hydrolysis na ⊃3;H-Leu-enkephalin a gaban Livagen da Epitalon. Sakamakon ya nuna cewa Livagen da Epitalon sun hana enkephalin lalata enzymes a cikin jini na ɗan adam. Livagen ya fi tasiri fiye da wasu sanannun masu hana peptidase irin su puromycin, leupeptin, da D-PAM. An ƙirƙira madaidaicin tasirin hanawar kashi na Livagen da Epitalon, kuma ƙimar su IC₅₀ sun kasance 20 da 500 μM bi da bi. An kimanta hulɗar tsakanin peptides da masu karɓa na opioid ta amfani da hanyar rediyoligand mai karɓa tare da [⊃3; H] [D-Ala⊃2;, D-Leu⁵] -enkephalin. Ba a sami ma'amala tsakanin μ ko δ masu karɓa na opioid a cikin ɓangaren membrane na kwakwalwar bera da gwajin peptides [2, 3].
A ƙarshe, ta hanyar daidaita maganganun kwayoyin halitta da inganta haɓakar furotin, Livagen yana nuna tasiri mai mahimmanci a cikin rigakafin tsufa, immunomodulation, da kariyar aikin hanta. Yana iya daidaita ayyukan enzymes masu narkewa, hana enkephalin masu lalata enzymes, da jinkirta tsufa ta salula ta hanyar kunna mahimman enzymes da kuma tasiri hanyar damuwa na oxidative. Bugu da kari, Livagen kuma yana inganta aikin rigakafi da matsayin antioxidant ta hanyar tasirin hepatoprotective kuma yana inganta dawo da aikin hanta a cikin cututtuka irin su hepatitis. Yana da yuwuwar ƙimar aikace-aikacen a cikin rigakafi da magance cututtukan da ke da alaƙa kuma yana ba da sabon jagorar bincike don rigakafin tsufa da kiyaye lafiya.
Game da Marubuci
Abubuwan da aka ambata a sama duk bincike ne, gyara su kuma Cocer Peptides ne suka haɗa su.
Mawallafin Jaridar Kimiyya
Vladimir Khavinson fitaccen masanin ilimin halittu ne na Rasha kuma farfesa wanda aka sani da binciken sa na farko a cikin magungunan peptide bioregulator da rawar da suke takawa wajen rage tsufa da inganta yanayin lafiya. Ya yi aiki a matsayin darektan Cibiyar Bioregulation da Gerontology ta St. Petersburg kuma ya kasance memba na Cibiyar Kimiyya ta Rasha. Bincikensa ya mayar da hankali kan haɓaka hanyoyin kwantar da hankali na peptide don haɓaka aikin rigakafi, kariya daga damuwa na oxidative, da haɓaka tsawon rai. Fiye da shekaru arba'in, ya hakowa tare da haɗa rukunin peptide da yawa, wanda ya haifar da gabatarwar magunguna na tushen peptide guda shida da ƙarin kayan abinci peptide 64 cikin aikin asibiti. Ayyukan Khavinson ya ba da gudummawa sosai ga fannin ilimin gerontology, musamman wajen fahimtar hanyoyin tsufa da haɓaka ayyukan da za a inganta rayuwa a cikin tsofaffi.
Vladimir Khavinson sanannen masanin ilimin gerontologist ne kuma farfesa, wanda ya shahara don bincikensa mai zurfi akan peptide bioregulator da rawar da suke takawa wajen rage tsufa da haɓaka tsawon lafiya. Ya rike mukamin darekta a Cibiyar Nazarin Halittu da Gerontology ta St. Petersburg kuma ya kasance memba na Cibiyar Kimiyya ta Rasha. Bincikensa ya dogara ne akan ci gaba da hanyoyin kwantar da hankali na peptide don haɓaka aikin rigakafi, tsayayya da damuwa na oxidative, da sauƙaƙe tsawon rai. A cikin fiye da shekaru arba'in na bincike, ya fitar da hada nau'ikan hadaddun peptide iri-iri, waɗanda suka haɓaka aikace-aikacen asibiti na magungunan peptide guda shida da ƙarin kayan abinci na tushen peptide 64. Ayyukan Khavinson sun ba da gudummawa mai mahimmanci ga fannin ilimin gerontology, musamman ma a fahimtar hanyoyin tsufa da haɓaka ayyukan da za a inganta rayuwar tsofaffi. An jera Vladimir Khavinson a cikin ambaton ambaton [5].
