CJC 1295 nwere DAC 5mg

▎ CJC1295 nwere DAC Structure

▎ CJC1295 na nyocha DAC

Kedu ihe ndabere nyocha nke CJC1295 na DAC?

Ngwa nke homonụ na-ewepụta homonụ uto ọdịnala (GHRH) na ọgwụgwọ ejedebere site na obere oge ọrụ ya. Nke a emeela ka ndị na-eme nchọpụta na-achọ ihe analog nke hormone na-ewepụta oge na-eto eto iji meziwanye irè ọgwụgwọ na ịdị mma. Site na nchụso ndị mmadụ maka ahụike na ịma mma, nkà mmụta ọgwụ ọha n'ịntanetị ewepụtala mkpali maka ụzọ mkpirisi iji nweta nkwalite ahụ ike, mbelata abụba, na akpụkpọ ntorobịa. N'ime ihe ndabere a, analogues homonụ uto sịntetịt dị ka CJC1295 na DAC adọtala ntị. Nnyocha na-egosi na ndị na-arụ ọrụ forum na-egosipụta nkà na ahụmahụ n'ime ọtụtụ ihe eji eme ihe, na ebumnuche maka ịhọrọ iji ihe ndị dị otú ahụ gụnyere oke ibu, nkwalite ahụ ike, mmeghari akpụkpọ ahụ, ụra ka mma, na ọgwụgwọ ọnya, wdg ^[1] . Dịka analog nke homonụ na-ewepụta homonụ na-eto ogologo oge, mmepe nke CJC1295 Na DAC dabere na nghọta miri emi nke usoro nchịkwa nke homonụ uto. Ọ bụ ezie na ọtụtụ ụmụaka nwere ụkọ hormone (GH) dịpụrụ adịpụ nwere nzaghachi uto dị mma na GHRH, ọkara ndụ nke GHRH dị mkpụmkpụ, na-egbochi mmetụta ọgwụgwọ ya. Iji dozie okwu a, ndị nchọpụta ewepụtala CJC1295 Na DAC, analog GHRH na-eme ogologo oge. Nnyocha egosiwo na ọ na-agụnye otu maleimide na-arụ ọrụ na C-terminus, na-enye ya ohere ijikọ protein protein dị ka serum albumin, si otú ahụ na-abawanye ọkara ndụ ya na inwe ike ịkwalite mmepụta hormone na-eto eto n'ime mmadụ ihe karịrị ụbọchị isii ka otu nchịkwa gasịrị ^.

Kedu usoro nke CJC1295 na DAC na ọgwụgwọ nke ọrịa dị iche iche?

Ntọhapụ nke homonụ na-akpali akpali: 

CJC1295 na DAC bụ ọgwụ sitere na peptide nke nwere ike ịkpalite mmepụta nke homonụ na-eto eto (GH) site na gland pituitary ^{[2, 3]} Ọ na-etinye otu maleimide na-arụ ọrụ na C-terminus, na-enye ya ohere ijikọ na protein protein plasma dị ka serum albumin. Njikọ a na-enye CJC1295-protein conjugate ogologo ndụ ogologo ndụ ma na-enyere ya aka ịkwalite mmepụta GH n'ime mmadụ ihe karịrị ụbọchị isii mgbe otu nchịkwa gasịrị. Nnyocha egosiwo na ntụtụ subcutaneous nke CJC1295 na DAC nwere ike ime ka mmụba na-adịgide adịgide, nke na-adabere na dose na ọkwa nke GH na insulin-like growth factor I (IGF-I) na ndị okenye ahụike ^{[2, 3]} Mgbe otu injection nke CJC1295 na DAC, nkezi plasma GH ịta ahụhụ mụbara site na 2-10 ugboro ma dịgidere maka 6 ụbọchị ma ọ bụ ogologo oge plasma. oge na-adịgide adịgide maka ụbọchị 9-11. Atụmatụ ọkara ndụ nke CJC1295 na DAC bụ ụbọchị 5.8-8.1. Mgbe ọtụtụ nchịkwa gasịrị, nkezi ọkwa IGF-I nọgidere n'elu ọkwa ntọala ruo ụbọchị 28.

Ọrụ na erughi homonụ nke uto: 

N'ụdị anụmanụ nwere ụkọ homonụ na-eto eto (GH), CJC1295 Na DAC egosila mmetụta dị mma. Maka ụmụ oke nwere mkpụrụ ndụ GHRH, ụmụ anụmanụ na-anata ọgwụ kwa ụbọchị nke CJC1295 na DAC gosipụtara ịdị arọ ahụ nkịtị na ogologo ahụ ^[4] Oke a na-emeso kwa awa 48 na 72 nwere oke ahụ na ogologo ahụ karịa anụmanụ ndị a na-emeso placebo. N'agbanyeghi na emeputaghi itolite n'uto zuru oke, egosiri ufodu nkwalite. N'ime otu ọgwụgwọ niile, oke ahụ na-adaba adaba na oke abụba subcutaneous bụ ihe kwesịrị ekwesị. CJC1295 na DAC mekwara ka mmụba na mkpokọta pituitary RNA na GH mRNA, na-egosi uto nke mkpụrụ ndụ somatotroph, nsonaazụ nke onyonyo immunohistochemical gosipụtara.

Kedu ka CJC1295 na DAC si enweta mmetụta na-adịgide adịgide n'ihe gbasara nhazi?

Njikọ nke covalent na serum albumin: 

CJC-1295 na-ejikọta otu maleimide na-arụ ọrụ na C-terminus, nke na-enye ohere ka ihe ahụ na-ejikọta ya na protein plasma dị ka serum albumin ^[2] Njikọ a na-enyere CJC-1295 aka ịnọgide na-arụsi ọrụ ike n'ime ọbara, na oge ọrụ ya dị ogologo oge karịa nke ọgwụ peptide na-abụghị conjugated ngwa ngwa. Ozugbo ejikọtara ya na serum albumin, CJC-1295 na-etolite mgbagwoju anya siri ike, si otú a na-agbatị ogologo oge ọrụ ya n'ime ahụ. Dịka ọmụmaatụ, mgbe otu maleimide nke CJC-1295 jikọtara ọnụ na akpịrị na-akwụghị ụgwọ na serum albumin, ọkara ndụ ya n'ime ọbara na-abawanye nke ukwuu. Usoro njikọ a pụrụ iche n'etiti peptides na-arụ ọrụ ma na-atọkwa ntọala maka mmetụta na-adịgide adịgide nke CJC-1295 ^[5].

Na-akwalite nkwụsi ike: 

Atọ maleimide usoro e synthesized site modifying ụmụ mmadụ ibu hormone-na-ahapụ ihe (hGRF), n'etiti nke CJC-1295 bụ a tetra-dochiri ụdị hGRF (1-29), na Nε-3-maleimidopropionamide ewepụtara nke lysine agbakwunyere na C-J- ^5.6 na-eme ka Ọdịdị nke C-J-19. vitro kwụsie ike megide dipeptidyl peptidase-IV. N'ime nyocha nzuzo nke homonụ nke uto nke mkpụrụ ndụ pituitary oke zụlitere, serum albumin atọ niile na-arụ ọrụ n'ụzọ ndụ. Mgbe a gbanyere usoro maleimide n'okpuru akpụkpọ ahụ naanị ya n'ime oke ụmụ nwoke Sprague Dawley nkịtị, ahụrụ nnukwu mmepụta homonụ uto na plasma. N'ime ha, CJC-1295 gosipụtara mmụba nke okpukpu anọ na mpaghara n'okpuru usoro nke hormone uto ma e jiri ya tụnyere hGRF (1-29) n'ime awa 2. Na mgbakwunye, nyocha Western blot nke plasma nke oke nke etinyere na CJC-1295 gosipụtara ọnụnọ nke CJC-1295 immunoreactive n'ime band nke kwekọrọ na serum albumin, nke pụtara nkeji iri na ise ka ịgba ọgwụ mgbochi ahụ wee nọgide na-ekesa ihe karịrị awa 24. Nsonaazụ ndị a na-egosi na CJC-1295 bụ ihe kwụsiri ike ma na-arụ ọrụ hGRF (1-29) analog nwere ogologo ndụ ọkara ndụ plasma ^[6].

Mmetụta nke CJC-1295 na mkpokọta ahụ wt (TBW; pụtara ± SE). Na njedebe nke oge ọgwụgwọ 5-wk, otu CJC/24 h gosipụtara TBW zuru oke. CJC / 48 h na CJC / 72 h dị iche iche ruru TBW dị elu nke ukwuu ma e jiri ya tụnyere ndị GHRHKO / pbo, ọ bụ ezie na ọ dị ntakịrị karịa na HTZ / pbo otu.

Isi mmalite: PubMed ^[4]

Gịnị bụ ngwa ụlọ ọgwụ nke CJC1295 na DAC?

CJC1295 Na DAC nwere ike kpalie nke ọma na nzuzo nke hormone ibu (GH). Ọtụtụ ọmụmụ egosila na ọgwụ a nwere ike ịbawanye mkpokọta plasma GH na ndị okenye nwere ahụike na-adabere na dose ^[3] . Dịka ọmụmaatụ, n'ime ọmụmụ ihe gbasara ahụike ahụike, ndị nọ n'agbata afọ 21 na 61 nwere nkezi plasma GH ntinye uche na-abawanye ugboro 2-10 maka ụbọchị 6 ma ọ bụ karịa mgbe ha natachara nchịkwa subcutaneous nke CJC1295 na DAC. Mmetụta a nke na-akpali mmepụta nke hormone uto bụ ihe dị ukwuu maka ndị ọrịa nwere ụkọ hormone ibu.

Maka ụmụaka nwere ụkọ homonụ na-eto eto, ọ bụ ezie na ọtụtụ ụmụaka nwere nzaghachi uto dị mma na hormone na-ahapụ hormone uto (GHRH), obere ndụ ọkara nke GHRH na-egbochi ngwa ụlọ ọgwụ ya. Dịka analọg GHRH na-eme ogologo oge, CJC1295 na DAC nwere ike ịhọrọ nke ọma jikọtara ya na albumin endogenous, si otú a na-agbatị ọkara ndụ ya na oge ọrụ ya. N'ime ọmụmụ nke ụmụ oke GHRH gene knockout (GHRHKO), a chọpụtara na nchịkwa otu ugboro kwa ụbọchị nke CJC1295 na DAC nwere ike ịnọgide na-enwe ịdị arọ ahụ na ogologo ahụ nke ụmụ oke, na-egosi na ọgwụ a nwere uru ngwa ngwa n'ịgwọ ụkọ hormone na-eto eto ^[4]. .

CJC1295 Na DAC nwere ike ọ bụghị nanị na-akpali mmepụta nke hormone na-eto eto kamakwa ịbawanye ọkwa nke insulin-dị ka ihe na-eto eto I. N'ọmụmụ ihe gbasara ụlọ ọgwụ, mgbe otu injection nke CJC1295 na DAC, nkezi plasma IGF-I ntinye uche na ndị okenye nwere ahụike mụbara site na 1.5-3 ugboro n'ime ụbọchị 9-11. Na mgbe ọtụtụ nchịkwa gasịrị, nkezi IGF-I larịị nwere ike ịnọ n'elu ntọala ruo ụbọchị 28 ^[4] . IGF-I nwere ọrụ dịgasị iche iche dị mkpa nke physiological dị n'ime ahụ mmadụ, gụnyere ịkwalite mgbasawanye cell, ọdịiche, na ndụ, na ịchịkwa metabolism, wdg. Ya mere, ịba ụba nke IGF-I nwere ike inwe mmetụta dị mma na ọgwụgwọ ọrịa dị iche iche.

Kedu ngwa nwere ike nke CJC1295 na DAC na ngalaba ahụike yana metụtara ya?

erughi homonụ nke uto: 

CJC1295 na DAC nwere ike na-akpali pituitary gland na-ezobe hormone uto. Maka ndị na-arịa ọrịa na-ezughị ezu na-emepụta hormone na-eto eto n'ihi ihe dị iche iche, karịsịa ụmụaka na ndị nọ n'afọ iri na ụma, ọ nwere ike imeziwanye ọnọdụ nke uto na mmepe mmepe ma kwalite uto ịdị elu. N'ịgwọ ụkọ homonụ nke uto, usoro ọgwụgwọ nnọchi homonụ uto ọdịnala enwetala nsonaazụ ụfọdụ, mana CJC1295 Na DAC nwere uru pụrụ iche. Ọ nwere ike jikọta ya na albumin endogenous, si otú a na-agbatị ọkara ndụ ya na oge ọrụ ya ^[4] . Dịka ọmụmaatụ, n'ime ọmụmụ nke ụmụ oke GHRH gene knockout ụmụ oke, a chọpụtara na nchịkwa kwa ụbọchị nke CJC-1295 ọgwụgwọ nwere ike ịnọgide na-enwe usoro ahụ nkịtị na uto nke ụmụ oke GHRHKO, gụnyere ịdị arọ ahụ nkịtị, ogologo ahụ, ogologo anụ ahụ, na abụba subcutaneous, na n'otu oge ahụ nwekwara ike ime ka mmụba na mkpokọta pituitary RNA na GH-mRNA, na-akwado mmepụta nke RNA na GH-MRNA, na-akwado mmepụta nke Alsectari. 2006).

Maka ndị ọrịa toro eto nwere ụkọ homonụ na-eto eto, CJC1295 Na DAC nwere ike ọ bụghị naanị melite nhazi ahụ, mụbaa oke akwara, ma belata abụba anụ ahụ kamakwa melite ogo ndụ. Hormone uto na-arụkwa ọrụ dị mkpa na ahụ okenye. Ọ na-etinye aka na usoro metabolic nke protein, abụba na carbohydrates. Enweghị homonụ na-eto eto ga-eduga ná nsogbu ndị dị ka ụbara abụba anụ ahụ, mbelata ahụ ike, na mbelata njupụta ọkpụkpụ. Ojiji nke CJC1295 na DAC nwere ike mezie mmebi ndị a ruo n'ókè ụfọdụ ma melite ọnọdụ anụ ahụ nke ndị ọrịa.

mgbochi ịka nká: 

Site na mmụba nke afọ, nzuzo nke hormone na-eto eto nke mmadụ na-eji nwayọọ nwayọọ na-ebelata, nke ga-eme ka mgbanwe dị iche iche na-eme ka ịka nká gbanwee. CJC1295 Na DAC nwere uru ngwa nwere ike na mgbochi ịka nká. Ọ nwere ike ịbawanye secretion nke hormone na-eto eto ruo n'ókè ụfọdụ, nke na-enyere aka melite ọdịda nke ọrụ anụ ahụ metụtara ịka nká. Dịka ọmụmaatụ, ọ nwere ike ịbawanye njupụta ọkpụkpụ. Hormone na-eto eto nwere ike ịkwalite ọrụ osteoblasts, mee ka ọkpụkpụ ọkpụkpụ dịkwuo elu, ma gbochie ọkpụkpụ ọkpụkpụ; welie ike akwara. Hormone na-eto eto nwere ike ịkpalite njikọ protein muscle, mee ka ọkpụkpụ anụ ahụ na ike dịkwuo elu; melite akpụkpọ elasticity na luster. Hormone na-eto eto nwere ike ime ka akpụkpọ ahụ dịkwuo ike ma na-agbanwe site n'ịkwalite njikọ collagen ^[4].

Ọgwụ egwuregwu na mmezigharị: 

CJC1295 Na DAC nwere ngwa nwere ike imezi mmerụ egwuregwu yana nkwalite arụmọrụ egwuregwu. Ọ nwere ike ịkwalite nrụzi ahụ na mmụgharị ma mee ka ọgwụgwọ ọnya dịkwuo ngwa. Mgbe onye na-eme egwuregwu merụrụ ahụ, ahụ kwesịrị ịrụzi anụ ahụ mebiri emebi ozugbo enwere ike iji weghachi ikike egwuregwu. Hormone uto na-ekere òkè dị mkpa na usoro a. Ọ nwere ike ịkpalite mmụba cell na ọdịiche dị iche iche, na-akwalite njikọ protein, ma mee ngwa ngwa nrụzi akwara na anụ ahụ ndị ọzọ ^[4] . N'otu oge ahụ, CJC1295 na DAC nwere ike ịbawanye ike na ntachi obi ma kwalite ikike egwuregwu. Hormone na-eto eto nwere ike ime ka uru ahụ dịkwuo elu ma melite ikike nke akwara, si otú ahụ na-eme ka ike na ntachi obi nke ndị na-eme egwuregwu dịkwuo elu ^[4] .

Ọrịa metabolic: 

Maka ụfọdụ ọrịa metabolic dị ka oke ibu na nguzogide insulin, CJC1295 na DAC nwere ike imetụta metabolism ike na nkesa abụba ma melite mmetụta insulin site na ịhazi usoro ihe na-akpata hormone-insulin dị ka uto. Oke ibu bụ ọrịa metabolic a na-ahụkarị, na ihe omume ya metụtara enweghị ahaghị n'etiti oriri ume na oriri. Hormone na-eto eto nwere ike ịkwalite ndakpọ abụba ma mụbaa oriri ume, si otú a na-enyere aka ịchịkwa ibu ahụ. Nguzogide insulin na-ezo aka na mbelata nke mmetụta anụ ahụ na insulin, na-eduga n'ịbawanye ọkwa shuga dị n'ọbara. Hormone na-eto eto nwere ike imeziwanye mmetụta insulin ma belata ọkwa shuga dị n'ọbara ^[4].

N'ikpeazụ, CJC1295 na DAC egosila mmetụta dị mma n'ọtụtụ akụkụ n'ọhịa nke nhazi hormone nke uto. Ọ nwere uru dị mkpa ọ bụghị naanị na ọgwụgwọ nke ụkọ hormone nke uto kamakwa na ọgwụ egwuregwu na nhazigharị na nchọpụta mgbochi ịka nká. Usoro ya na-eme ogologo oge na-ebelata oge nchịkwa, yana ezigbo nchekwa ya na nnabata ya na-enyekwa nkwado siri ike maka ngwa ụlọ ọgwụ ya. Ọ bụ ezie na ọ ka nọ na nyocha nyocha n'akụkụ ụfọdụ dị ka mgbochi ịka nká, mmetụta ndị e gosipụtara na-ezuru ezu iji gosipụta uru ya pụrụ iche na atụmanya ngwa ngwa n'ọhịa nke sayensị ndụ na ubi ndị metụtara ya.

Banyere Onye dere ya

Ihe niile a kpọtụrụ aha n'elu bụ Cocer Peptides na-enyocha, dezie ma chịkọta ya.

Onye ode akwụkwọ akụkọ sayensị

Teichman SL bụ okachamara nwere ahụmahụ na nke nwere mmetụta na ngalaba ọgwụ na mmepe ọgwụ. O nwere njikọ chiri anya na ọtụtụ ụlọ ọrụ na ụlọ ọrụ ndị a ma ama, dị ka Organisation Concentr Analges Inc, Teichman Drug Dev Consulting, Wroclaw Medical University, BEAT Biotherapeut Corp, Corthera Inc na ụlọ ọrụ ndị metụtara ya Corthera, Chiasma, ARYx Therapeut Inc, wdg Ọzọkwa, ọ na-ejikọta ya na Athens Univ Hospating College na nkà mmụta ọgwụ ike na Athens Univ Hosp na nkà mmụta ọgwụ. omume. Mpaghara ọkachamara ya na-ekpuchi mpaghara dị iche iche gụnyere Cardiovascular System & Cardiology, General & Internal Medicine, Engineering, Endocrinology & Metabolism, na Pharmacology & Pharmacy, na-arụ ọrụ dị ịrịba ama na onyinye karịsịa na mmepe ọgwụ na biotherapy. Edepụtara Teichman SL na ntụaka ntụaka [3].

▎ Okwu ndị dị mkpa

[1] Van Hout MC, Hearne E. Netnography nke ụmụ nwanyị iji Hormone Growth Synthetic CJC-1295: Pulses and Potions[J]. Ihe eji eme ihe & eji eme ihe, 2016,51(1):73-84.DOI:10.3109/10826084.2015.1082595.

[2] Timms M, Ganio K, Steel R. Usoro maka ịkwado mmejọ CJC-1295 na equine plasma samples site na LC-MS/MS[J]. Nnwale na nyocha ọgwụ, 2019,11 (8): 1248-1257.DOI: 10.1002/dta.2599.

[3] Teichman SL, Neale A, Lawrence B, et al. Ogologo oge mkpali nke hormone na-eto eto (GH) na insulin-dị ka ihe na-eto eto m na-ezobe site na CJC-1295, analog na-arụ ọrụ ogologo oge nke hormone na-ahapụ GH, na ndị toro eto dị mma [J]. Akwụkwọ akụkọ nke Clinical Endocrinology & Metabolism, 2006,91 (3): 799-805.DOI: 10.1210 / jc.2005-1536.

[4] Alba M, Fintini D, Sagazio A, et al. Nlekọta otu ugboro kwa ụbọchị nke CJC-1295, homonụ na-ewepụta homonụ na-ewepụta ogologo oge (GHRH), na-eme ka uto nke GHRH knockout òké [J]. Akwụkwọ akụkọ American nke Physiology-Endocrinology na Metabolism, 2006,291(6):E1290-E1294.DOI:10.1152/ajpendo.00201.2006.

[5] Timms M, Ganio K, Forbes G, et al. Ihuenyo mmeghachi omume chain polymerase maka nchọpụta nke CJC-1295 na analogues hormone na-ewepụta homonụ na-eto eto na plasma equine[J]. Nnwale ọgwụ na nyocha, 2019,11 (6): 804-812.DOI: 10.1002/dta.2554.

[6] Jetté L, Léger R, Thibaudeau K, et al. Ihe na-ewepụta homonụ na-eto mmadụ (hGRF) _1-29 -albumin bioconjugates na-eme ka onye na-anabata GRF dị na pituitary n'ihu na oke:: Nchọpụta nke CJC-1295 dị ka analog GRF na-adịte aka [J]. Endocrinology, 2005,146(7):3052-3058.DOI:10.1210/en.2004-1286.

Edemede na ozi ngwaahịa niile enyere na webụsaịtị a bụ naanị maka mgbasa ozi na ebumnuche mmụta.  

Ngwaahịa ndị enyere na webụsaịtị a bụ naanị maka nyocha in vitro. A na-eme nyocha in vitro (Latin: * na iko *, nke pụtara na ihe eji enyo) na mpụga ahụ mmadụ. Ngwaahịa ndị a abụghị ọgwụ, ndị US Food and Drug Administration (FDA) akwadoghị ya, ha agaghịkwa eji ya gbochie, gwọọ, ma ọ bụ gwọọ ọnọdụ ahụike ọ bụla, ọrịa, ma ọ bụ ọrịa. Iwu amachibidoro nke ọma iwebata ngwaahịa ndị a n'ime ahụ mmadụ ma ọ bụ anụmanụ n'ụdị ọ bụla.