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1kits （10Ibikoresho）
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HCGStructure Imiterere ya
Inkomoko: PubChem |
IUPAC Yegeranye: N (1) Cys-Gly-OH.H-Aad (1) -OH Inzira ya molekulari: C 11H 19N 3O 6S. Uburemere bwa molekuline: 321.35g / mol Numero ya CAS: 9002-61-3 PubChem CID: 4369448 Synonyme: Chorionic gonadotrophin ； CHEMBL1233255 |
Research Ubushakashatsi bwa HCG
Ni ubuhe bushakashatsi bwakozwe na HCG?
Chorionic Gonadotropin yumuntu (HCG) ni molekile ifite imirimo myinshi yingenzi kandi igira uruhare runini mubice nko gutwita na kanseri yabantu. Ubwa mbere, HCG ni molekile ikabije. Ni glycoproteine ya acide cyane kandi irimo isukari nyinshi. Icya kabiri, HCG ibaho muburyo butandukanye, harimo HCG isanzwe, sulfate HCG, hyperglycosylated HCG, ubuntu β-HCG, na hyperglycosylated free β-HCG, nibindi [1].
Kubijyanye no gutwita, HCG ningirakamaro mugihe cyimihango yumuntu no gutwita kwabantu. HCG isanzwe igira uruhare mukuzamura umusaruro wa corpus luteum progesterone kandi ikagira kandi ibikorwa byingenzi mugutandukanya selile trophoblast no kugaburira inda. Kurugero, itahura itangwa ryimirire yibyara binyuze muri angiogenezi mumitsi ya spiral ya myometrium [1] . Hyperglycosylated HCG nayo igira uruhare runini mugihe cyambere cyo gutwita. Niyo isoform nyamukuru ya HCG mugihe cyambere cyo gutwita, ishobora kubuza apoptose, gutera kwibasira ingirabuzimafatizo, gukura, no guhinduka nabi, no kugenzura iyatewe no gukura kw'ibibyimba [1].
Ku bijyanye na kanseri, ibibyimba bitari trophoblastique bibyara hyperglycosylated free β subunit ya HCG, ibyo bikaba nk'impamvu ya autocrine, bikomeza guteza imbere gukura no guhindura nabi ingirabuzimafatizo za kanseri mu kurwanya apoptose [1].
Byongeye kandi, gutahura HCG bikoreshwa cyane mu bikorwa by’amavuriro, harimo gupima inda, gukurikirana ingaruka z’inda zitwite, kumenya ibyago by’inda zanduye syndrome de Down, guhanura preeclampsia, gutahura indwara ya pitoito HCG, gutahura no gucunga indwara ziterwa na trophoblastique, gusuzuma indwara zifata imyanya ndangagitsina ya trophoblastique, kugenzura ibibyimba bya mikorobe ya selile na virusi ,.
Ni ubuhe buryo bwo gukora bwa HCG?
1. Ingaruka ku kwakirwa kwa endometrium
Ubushakashatsi bumwe bwerekanye ko HCG ishobora kugira ingaruka ku iyakirwa rya endometrium binyuze muri miR-126-3p / PIK3R2 / PI3K / Akt / eNOS kandi ikagira uruhare runini mu gikorwa cyo gutera urusoro (Wang W, 2023). Ibigaragara byihariye ni ibi bikurikira:
Kunoza imikorere yimikorere ya endometrale: Mugushiraho imbeba yimbeba yo kudakora neza kwa emboro (EID) no kuyivura hamwe na mifepristone, kandi icyarimwe ikora ubushakashatsi kumasemburo ya epitheli yumuntu (EECs), byagaragaye ko nyuma yo kuvura HCG, imitekerereze ya endometrium mu mbeba za EID yarushijeho kunozwa. Kurugero, urwego rwo kwerekana CD105 mu mbeba hamwe na poroteyine ya cadherine CD144 na CD146 byariyongereye nkuko byagenwe na immunohistochemie na Western blotting.
Kugena imvugo ya gene: HCG irashobora guteza imbere imvugo ya miR-126-3p kandi ikabuza imvugo ya PIK3R2, na miR-126-3p intego PIK3R2. Haba muri vivo no mubushakashatsi bwa vitro byagenzuye ko HCG ikora inzira ya PI3K / Akt / eNOS inyuze muri miR-126-3p / PIK3R2, bityo bigatuma imitekerereze ya endometrium ikomera.
2. Uruhare rwo gukomeza gutwita
HCG ikorwa cyane cyane na selile syncytiotrophoblast itandukanye kandi nikimenyetso gikomeye cyo gusama gikenewe mugukomeza gutwita [2].
Gukora ibimenyetso byinshi byerekana ibimenyetso: HCG irashobora guhuza imisemburo ya luteinizing / chorionic gonadotropin reseptor (LHCGR) kandi irashobora gukora ibimenyetso byinshi byerekana ibimenyetso, harimo nababyeyi barwanya decapentaplegic homologique 2 (Smad2) kubabyeyi bafite ubushobozi bwinshi, imikoranire ya protein kinase C (PKC), hamwe na / cyangwa protein kinase A (PKA).
Guteza imbere angiogenez muri endotelium ya nyababyeyi: HCG igira uruhare runini mugutezimbere angiogenez muri endotelium ya nyababyeyi, itanga ahantu heza ho gukura ku isoro.Gukomeza gutuza kwa myometrium: Ifasha kugumya guhagarara neza kwa nyababyeyi kandi bigatanga ibidukikije bikwiye mu mikurire y’inda ikingira. Imigaragarire-nyababyeyi kandi iringaniza igisubizo cyimikorere yumubiri wa nyababyeyi ku isoro.
3. Uruhare rwo kwimura urusoro rwahagaritswe
Ubushakashatsi bwerekanye ko amashanyarazi akoreshwa mu gukwirakwiza amashanyarazi (TEAS) hamwe no kuvura HCG bishobora guteza imbere ingaruka z’inda z’abarwayi barimo kwimura urusoro rwahagaritswe [3].
Kongera umubyimba wa endometrium: Kuvura TEAS bifasha cyane cyane kongera umubyimba wa endometrium kubarwayi barimo kwimura urusoro rwahagaritswe. Kuvura hamwe hamwe na HCG na TEAS nabyo bifasha kongera umubyimba wa endometrium muri aba barwayi.
Kugabanya igipimo cyamaraso yimitsi ya nyababyeyi: Indangagaciro zamaraso ya endometrale yamaraso PI na RI muri TEAS zifatanije nitsinda rya HCG hamwe nitsinda rya TEAS ryaragabanutse cyane ugereranije n’itsinda rishinzwe kugenzura, byerekana ko kuvura hamwe HCG na TEAS bishobora kugabanya igipimo cy’amaraso PI na RI y’imitsi y’inda y’abarwayi.
Kongera urwego rwibintu bijyanye no gufata neza inda muri serumu: Kuvura HCG bifasha cyane cyane kongera urugero rwa serumu P na leukemia inhibitory factor (LIF) kubarwayi barimo kwimura urusoro rwakonje. Urwego rwa serumu LIF mu itsinda rya TEAS, itsinda rya HCG, hamwe na TEAS ihujwe nitsinda rya HCG byose byari hejuru cyane ugereranije nabari mumatsinda yo kugenzura. Igipimo cyo gutera urusoro mu itsinda ryahujwe cyari hejuru cyane ugereranije n’itsinda rishinzwe kugenzura, byerekana ko kuvura hamwe TEAS na HCG bishobora kuzamura ingaruka z’inda z’abarwayi batewe no kwimura urusoro.
4. Kuvura hypogonadism y'abagabo
Hypogonadism iterwa no gusohora kudahagije kwa gonadotropine ku bagabo igira ingaruka zikomeye ku buzima bw'imyororokere ndetse n'ubuzima bw'abarwayi. Chorionic muntu Gonadotropin (HCG) igira uruhare runini mu kuvura izo ndwara. HCG ihuza reseptors hejuru yutugingo ngengabuzima twa Leydig muri testis, ikora inzira yerekana ibimenyetso byerekana ibimenyetso, kandi igatera ihinduka rya cholesterol muri testosterone. Ubwiyongere bwa testosterone burashobora guteza imbere imikurire niterambere rya testis no kongera ubwinshi bwa testis. Muri icyo gihe, testosterone irashobora kandi gutera imbaraga mu mikurire yimyororokere nka epididymis na vas deferens. Testosterone igira uruhare runini mugutezimbere igitsina cya kabiri cyigitsina gabo. Mu kongera urwego rwa testosterone, kuvura HCG birashobora guteza imbere ubwanwa bwa pome na pome ya Adam, bikongerera imbaraga imitsi, kandi bikongera ubushake bwimibonano mpuzabitsina, nibindi
Inkomoko ya selile, intego, bifitanye isano na kasade yerekana ibimenyetso, n'imikorere ya isoforms zitandukanye za HCG kubagore badatwite kandi batwite.
Inkomoko: Byatangajwe [2]
Ni ubuhe buryo bukenewe bwa HCG?
1. Ikoreshwa mugupima gutwita hakiri kare
Agaciro ko gupima ibiri muri serumu T-HCG na β-HCG mugihe cyo gutwita hakiri kare: Kusanya ibibazo byo gutwita bidasanzwe hakiri kare, gupima serumu T-HCG ukoresheje chemiluminescence, gupima serumu β-HCG ukoresheje radioimmunoassay, kandi ukore ubushakashatsi bukomeye. Ibisubizo byerekana ko ibiri muri serumu T-HCG na β-HCG mu itsinda ryo gutwita kwa ectopique biri hasi cyane ugereranije n’abari mu itsinda ryo gukuramo inda ndetse n’itsinda rishinzwe gutwita. Ibi byerekana ko gupima ibiri muri serumu T-HCG na β-HCG bifite umwihariko mwiza nigipimo cyo gusuzuma cyo gutandukanya inda ya ectopique no gutwita imbere, kandi T-HCG ifite sensibilité kandi yizewe [4].
Gupima inda ya ectopique hamwe no kumenya hamwe serumu HCG, β-HCG, na progesterone: Menya serumu HCG, β-HCG, na progesterone kubagore basanzwe batwite nabagore batwite batwite ectopique. Ibisubizo byerekana ko urwego rwa serumu HCG, β-HCG, na progesterone ku bagore basanzwe batwite aruta urw'abagore batwite batwite ectopique. Iyo bigaragaye byonyine, igipimo cyukuri cya serumu HCG mugupima inda ya ectopique ni 70.83%; igipimo cyukuri cya β-HCG ni 66.7%; igipimo cyukuri cya progesterone ni 54.17%. Kumenyekanisha hamwe serumu HCG, β-HCG, na progesterone bifite igipimo cyukuri kigera kuri 95.8% mugupima inda ya ectopique [5].
2. Gushyira mubikorwa bya tekinoroji yimyororokere ifashwa
Kunonosora imikurire ya follicular: Mugukangura intanga ngore, menopausal gonadotropin (hMG) irashobora gukura neza mumikurire nyuma ya pituito desensisisation iterwa na gonadotropine irekura imisemburo ya hormone (Gn-RH-analogue). Ibi biterwa ahanini nuko birinda imisemburo idasanzwe ya endogenous luteinizing hormone (LG) ibaho mugihe cyo kuvura hMG hafi kimwe cya gatatu cyinzira yo kuvura. Mu bushakashatsi bwinshi bwakoresheje buserelin cyangwa degarelix nk'ikigereranyo cya Gn-RH, abarwayi 282 bakiriye mu gusama kwa vitro, kwimura gamete intrafallopian, cyangwa mu rwego rwo kuvura 'muri vivo '. Ubuvuzi hamwe hamwe na GnRH analogue / hMG / HCG bwongereye cyane igipimo cyo gutwita mumatsinda yose. Umubare w'inda z'abarwayi bavuwe na HMG / HCG wari 17%, mu gihe ubuvuzi hamwe bwatumye 25% by'abarwayi batwite [6].
Gutezimbere kwakirwa kwa endometrium: Chorionic muntu Gonadotropine (HCG) nikimwe mubimenyetso byingenzi mbere yo gutera urusoro. Ubushakashatsi bwakoresheje isesengura ry’imitsi ya endometrale bwerekanye ko nyuma y’ubuyobozi bwa HCG, umubare w’utugingo ngengabuzima tugaragaza molekile ya endothelial selile adhesion molekile VE-cadherin (CD144) na S-Endo-1 (CD146) wiyongereye ku buryo bugaragara, byerekana ko molekile zifata ingirabuzimafatizo zishobora kuba uburyo HCG itezimbere no gutera inda [7]..
2. Kuvura kubura luteal
Mu myaka 20 ishize, ubuyobozi bwa progesterone exogenous bwakoreshejwe nkigikoresho cya luteal phase (LPS) kijyanye no gukurura intanga ngore ikoresheje Chorionic Gonadotropin (HCG) kugirango abantu bakure neza. Itangizwa rya GnRHa kugirango ritere ovulation yerekana ko ubuyobozi bwa progesterone exogenous budahagije kugirango ugere ku gipimo gishimishije cyo gutwita nta HCG yongeyeho.
Ibi byatumye habaho ingamba zindi zo gushyigikira icyiciro cya luteal. Kongera umusaruro wa progesterone ya endogenous ya corpora lutea nyinshi ningingo yingenzi, kuruhande rumwe, kugirango wirinde iterambere rya syndrome de ovarian hyperstimulation, kurundi ruhande, kugirango itange urugero ruhagije rwa progesterone kugirango ikomeze guterwa.
Ubushakashatsi buriho bwasuzumye uruhare rwa micro-dose HCG yo gushyigikira icyiciro cya luteal kandi yiga ibyiza byayo nibibi. Hashingiwe ku miterere ya farumasi ya HCG, icyitegererezo cy’imibare yo gukwirakwiza kwibanda kwa HCG mu cyiciro cya luteal cyasuzumwe hifashishijwe uburyo butandukanye bw’ubuyobozi bwa HCG nk'inkunga ya luteal. Birasabwa ko inkunga yicyiciro cya luteal itangwa hamwe na GnRHa itera (ni ukuvuga 1500IU) irakomeye cyane, kandi ubuyobozi bwa micro-dose ya buri munsi HCG bushobora gutanga ubufasha bwiza bwa luteal kumiti iboneka ubu. Ibisubizo byubuvuzi byambere byuburyo bwa micro-dose HCG nabyo biratangwa [8].
3. Kuvura testis itabogamye imbere-munda yinda
Ubushakashatsi bwakorewe ku barwayi batewe na orchidopexy ya testis itabogamye mu nda-yo mu nda kuva muri Nzeri 2010 kugeza muri Nzeri 2016. Nyuma y'ibyumweru bibiri nyuma yo kubagwa, ababyeyi b'abarwayi bavuwe na hormone bari bakeneye gukurikiza gahunda y'ibyumweru 6. Abarwayi bahawe inshinge zo munsi ya 500 UI (Gonasi-HP) buri cyumweru. Gukurikirana byakozwe nyuma yubuvuzi nyuma y amezi 6.
Ingano ya testis yapimwe na ultrasound na ultrasonic elastografiya kuri buri gukurikirana kandi ugereranije n’abarwayi batavuwe. Ibisubizo byagaragaje ko abarwayi 45 bavuwe, bafite impuzandengo y’amezi 18.0 ± 9.7. Abarwayi 32 bahawe imiti ya hormone nyuma yo kubagwa, kandi nta reaction mbi cyangwa ibibazo byo kwikuramo.
Abarwayi bose barangije gukurikirana. Nta manza zatewe na testicular atrophy mumatsinda yombi. Mu itsinda ry’ubuvuzi, 81% by’abarwayi bageze ku bunini busanzwe bwa testis mu mezi 6, mu gihe ingano y’abandi barwayi yari ikiri nto. Mu itsinda ritavuwe, 46% by'abarwayi bageze ku bunini bwa testis. Gukoresha nyuma yubuvuzi bwa Chorionic Gonadotropine yumuntu (u-HCG) birashobora kunoza ingano nimikorere ya testis itera imbaraga zo gukura no gutera imbere kwa testis [9].
4. Gusaba mubushakashatsi bwurukingo rwa β-HCG
Kora ikizamini cyo gukangura HCG ku bagore bakingiwe urukingo rwa NII beta-HCG ariko bakaba badafite titeri ya antibody anti-HCG, kandi ukoreshe imbaraga zo gusohora serumu progesterone nk'ikimenyetso cyerekana igisubizo cya corpus luteum ku mitsi ya HCG. Ibisubizo byerekanye ko mu itsinda rishinzwe kugenzura, abagore benshi bari bafite urwego rwo hejuru rw’ururenda rwa progesterone nyuma yo gukanguka kwa HCG kurenza urwego rw’ibanze, mu gihe urwego rwo hejuru rwa progesterone ku bagore bo mu itsinda ryakingiwe rutarenze urwego rw’ibanze nyuma yo gukingirwa, byerekana ko antibodiyite zatewe nuru rukingo zishobora gukumira ingaruka za HCG zidasanzwe [10].
Mu gusoza, HCG igira uruhare rudasubirwaho mubuvuzi, cyane cyane mubice bijyanye n'imyororokere. Mu kuvura imyororokere, irashobora gutera intanga ngabo, bikazana ibyiringiro kubarwayi bafite ubugumba buterwa n'indwara ya ovulation. Muguhuza reseptor ya LH mumubiri, irashobora kuzuza urwego rwa LH rudahagije muri protocole ya antagonist kandi igafasha gukura neza. Kubura luteal, HCG irashobora gukangura corpus luteum kugirango isohore progesterone kandi ikomeze ibidukikije bikwiye kugirango itwite.
Kuri hypogonadism y'abagabo iterwa no gusohora kudahagije kwa gonadotropine, HCG irashobora gukangurira selile Leydig muri testis guhuza no gusohora testosterone, guteza imbere testicular, kunoza imiterere ya kabiri yimibonano mpuzabitsina, no kuzamura imikorere yimyororokere nubuzima bw abarwayi. Muri icyo gihe, HCG igira uruhare runini mu gukomeza gutwita hakiri kare, itera corpus luteum guhora isohora progesterone kugira ngo urusoro rushobore gutera imbere. Mu isuzuma ry’amavuriro, kumenya urwego rwa HCG ni ishingiro ry’ingenzi mu kumenya gutwita hakiri kare no kumenya inda zidasanzwe nko gutwita kwa ectopique, gutanga inkunga ikomeye yo gutabara no kuvurwa ku gihe.
Ibyerekeye Umwanditsi
Ibikoresho byavuzwe haruguru byose byakorewe ubushakashatsi, byahinduwe kandi byakozwe na Cocer Peptides.
Umwanditsi w'ikinyamakuru
Chinedu Nwabuobi ni umushakashatsi ufite ubuhanga mu bijyanye no kubyara & Gynecology, Imyororokere y’ibinyabuzima, Biochemie & Molecular Biology, Chemistry, na Oncology. Yifatanije n’ibigo byinshi bizwi, birimo kaminuza y’amajyepfo ya Floride, kaminuza ya Rochester, n’ikigo cy’urwibutso rwa Sloan Kettering. Ubushakashatsi bwe bwibanze ku ngingo nk'imikorere y'ibinyabuzima no gukoresha amavuriro ya chorionic gonadotropine ya muntu (HCG), atanga umusanzu mu gusobanukirwa uruhare rwayo mu buzima bw'imyororokere hamwe na patologi bifitanye isano. Chinedu Nwabuobi yanditse murutonde rwibisobanuro [2].
