By Cocer Peptides 
Watanni 1 da suka gabata
DUKKANIN LABARI DA BAYANIN KYAUTATAWA DA AKE BAYAR A WANNAN SHAFIN KAWAI DOMIN WATSA BAYANI NE DA MANUFOFIN ILIMI.
Kayayyakin da aka bayar akan wannan gidan yanar gizon an yi niyya ne kawai don binciken in vitro. Binciken in vitro (Latin: *a cikin gilashi*, ma'ana a cikin gilashin gilashi) ana gudanar da shi a wajen jikin mutum. Waɗannan samfuran ba magunguna ba ne, Hukumar Abinci da Magunguna ta Amurka (FDA) ba ta amince da su ba, kuma dole ne a yi amfani da su don hanawa, magani, ko warkar da duk wani yanayi na likita, cuta, ko cuta. Doka ta haramta shigar da waɗannan samfuran cikin jikin mutum ko dabba ta kowace hanya.
Dermorphin shine peptide mai ƙarfi na opioid wanda aka ware daga fatar wasu nau'in kwadi.
Kayan aikin Dermorphin's Analgesic Effects
Yin hulɗa tare da masu karɓar Opioid
Dermorphin da farko yana yin tasirin analgesic ta hanyar hulɗa tare da mai karɓar μ-opioid (MOP). Mai karɓa na μ-opioid shine babban memba na dangin mai karɓar opioid kuma yana taka muhimmiyar rawa wajen daidaita yanayin zafi. Dermorphin da analogues ɗin sa na iya ɗaure musamman ga mai karɓar MOP, daidai da maɓalli mai dacewa a cikin kulle, don haka kunna hanyoyin sigina na ƙasa. [Cys (ATTO 488) 8] Dermorphin - NH₂ (DermATTO488), wani novel fluorescent Dermorphin analog wanda ya danganta da ATTO488, yana ɗaure zuwa sel HEK da CHO suna bayyana masu karɓar MOP na ɗan adam kuma suna nuna halaye masu kama da Dermorphin. Yana ƙarfafa daurin sunadaran G, yana haɓaka haɗin GTPγ [35S], ta haka yana kunna jigilar siginar da ke da alaƙa, kuma a ƙarshe yana hana watsa siginar zafi. Dermorphin kuma yana ƙarfafa ERK1 / 2 phosphorylation, yana ƙara rinjayar tsarin nazarin halittu na ciki da kuma shiga cikin tsarin tasirin analgesic.
Hana watsa siginar jin zafi a matakin kashin baya
Nazarin gwaje-gwaje ya nuna cewa Dermorphin yana hana watsa siginar jin zafi a matakin kashin baya. A cikin gwaje-gwajen, jiko na 0.16 mg / kg Dermorphin ya haifar da karuwa mai mahimmanci da ci gaba a cikin ma'auni mai mahimmanci a cikin masu aikin sa kai masu lafiya, kuma wannan tasirin ya kasance daidai da magana a cikin batutuwa tare da cikakken ciwo na kashin baya, yana nuna cewa Dermorphin da farko yana hana watsawar nociceptive a matakin kashin baya. Wannan tsarin aikin na iya kasancewa yana da alaƙa da tsarin Dermorphin na sakin neurotransmitter a cikin kashin baya ko aikin sa kai tsaye akan ƙwayoyin jijiyoyin kashin baya. Kashin baya yana aiki a matsayin mahimmanci don watsa siginar ciwo, kuma tsarin aikin Dermorphin na aikin sa yana hana haɓakar watsa siginar ciwo zuwa kwakwalwa, don haka yana samun sakamako na analgesic.
Halayen aikin mai karɓa na musamman
Ko da yake naloxone na iya gaba ɗaya gaba da tasirin morphine da analogues na opioid, zai iya jujjuya tasirin hanawa na Dermorphin a kan nociceptive spinal reflexes. Wannan yana nuna cewa Dermorphin yana hulɗa tare da ƙungiyoyi daban-daban na masu karɓar opioid na kashin baya yayin haifar da analgesia, kuma tsarin aikinsa ya bambanta da na magungunan opioid na gargajiya. Wannan yanayin aiki yana ba da wasu fa'idodi a cikin aikace-aikacen analgesic, kamar rage tasirin sakamako na gama gari masu alaƙa da magungunan opioid na gargajiya.
Ayyukan Analgesic na Dermorphin a Gwajin Dabbobi
Ingantacciyar Analgesia a cikin Samfuran Dabbobi da yawa
A cikin nau'ikan ciwon daji daban-daban, Dermorphin ya nuna tasirin analgesic mai ƙarfi. A cikin gwaje-gwaje irin su wutsiya, farantin zafi, tsunkule wutsiya, formalin, da gwaje-gwajen rubutu na acetic acid, gudanarwar intraperitoneal na Dermorphin da peptides masu alaƙa sun rage jin daɗin dabbobi ga ciwo kuma suna ƙara ƙofofin zafi. Waɗannan gwaje-gwajen sun kwaikwayi zafi na nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan]. A cikin gwajin farantin zafi, bayan an ba da Dermorphin ga dabbobi, lokacin latency don lasar tafin hannu ko tsalle ya daɗe sosai, yana nuna raunin raɗaɗin raɗaɗi ga haɓakawar thermal, don haka yana nuna kyakkyawan sakamako na analgesic na Dermorphin.
Fa'idodin Kwatancen Kan Sauran Magungunan Analgesic
Idan aka kwatanta da magungunan analgesic na gargajiya irin su morphine, Dermorphin yana nuna fa'idodi masu mahimmanci a wasu fannoni. A cikin nau'ikan dabbobi, bayan gudanarwar intracerebroventricular, Dermorphin ya nuna ƙarin zaɓi da tasirin analgesic fiye da morphine, tare da tsawon lokaci na aiki. Wasu hadewar Dermorphin tetrapepetides kuma sun nuna babban aikin analgesic, tare da karfin analgesic kusan sau 1,500 da sau 17 na morphine bayan gudanarwar intracerebroventricular ko subcutaneous a cikin mice, bi da bi. Waɗannan fa'idodin suna ba da kyakkyawan fata don aikace-aikacen asibiti na Dermorphin a cikin analgesia, mai yuwuwar magance gazawar magungunan analgesic na gargajiya, kamar ƙarancin ƙarancin analgesic ko tsawon lokaci.
Bincike da Binciken Aikace-aikacen Dermorphin a cikin Mutane
Sakamakon Gwaji na Farko
A cikin 1985, bazuwar, gwajin gwaji na asibiti akan amfani da Dermorphin don ciwon bayan tiyata ya nuna cewa Dermorphin da aka gudanar ta hanyar allurar intrathecal ya fi placebo da morphine (wanda aka yi amfani da shi azaman fili) dangane da tasirin analgesic. Wannan bincike mai mahimmanci ya ba da shaida mai mahimmanci don aikace-aikacen asibiti na Dermorphin a cikin analgesia, yana nuna babbar damarsa a cikin kula da ciwo na baya.
Yanayin Aikace-aikacen Mai yuwuwar
Gudanar da Ciwon Ciki na Ƙarfafawa: Ƙwararrun ƙwayar cuta na iya haifar da ciwo mai tsanani, yana tasiri sosai ga farfadowar marasa lafiya da ingancin rayuwa. Dermorphin ya nuna inganci wajen rage jin zafi a farkon gwajin asibiti ya sanya shi a matsayin sabon zaɓi na analgesia mai ban sha'awa. Idan aka kwatanta da magungunan analgesic na gargajiya na baya-bayan nan, tsarinsa na musamman na aiki da fa'idodi-kamar rage haɗarin haƙuri da dogaro da ƙwayoyi-bayar da marasa lafiya mafi aminci kuma mafi inganci maganin analgesic.
Maganin jin zafi na yau da kullum: Ga marasa lafiya da ciwo mai tsanani, musamman ma wadanda ke da mummunar amsa ga magungunan analgesic na gargajiya ko kuma suna da tasiri mai mahimmanci, Dermorphin na iya zama wani zaɓi mai mahimmanci na madadin magani. A cikin kulawa da ciwo mai tsanani a cikin marasa lafiya na ciwon daji, hanyoyin maganin halin yanzu suna da wasu ƙuntatawa. Bayyanar Dermorphin yana kawo sabon bege ga wannan yawan masu haƙuri. Its sakamako na analgesic na dogon lokaci zai iya mafi kyawun sarrafa ciwo mai ɗorewa a cikin marasa lafiya da ciwon daji da inganta yanayin rayuwarsu.
Kulawa da jin daɗi: A cikin saitunan kulawa na jin daɗi, rage jin zafi na marasa lafiya shine babban fifiko. Ƙaƙƙarfan abubuwan analgesic na Dermorphin sun sa ya zama ɗan takara mai ban sha'awa don rage jin zafi na ƙarshen rayuwa a cikin kulawar kwantar da hankali. Ta hanyar hanyoyin gudanarwa masu dacewa da gyare-gyaren kashi, zai iya taimakawa marasa lafiya su rage wahala da haɓaka ta'aziyya a lokacin mataki na ƙarshe na rayuwa.
Kammalawa
A matsayin abu mai mahimmanci na analgesic na musamman da kuma tasiri mai mahimmanci na analgesic, Dermorphin yana riƙe da aikace-aikace masu ban sha'awa a fagen kula da ciwo.
Sources
[1] Giakomidi D, Bird MF, McDonald J, et al. Ƙimar [Cys (ATTO 488) 8] Dermorphin-NH2 a matsayin kayan aiki na littafi don nazarin μ-opioid peptide receptors [J]. Plos Daya, 2021,16 (4): e250011.DOI:10.1371/journal.pone.0250011.
[2] Hesselink J, Schatman, M E. Sake gano tsofaffin magunguna: lamarin da aka manta na dermorphin don ciwon baya da jin zafi [J]. Journal of Pain Research, 2018,11:2991-2995.DOI:10.2147/JPR.S186082.
[3] Guzevatykh LS, Voronina TA, Emel'Ianova TG, et al. Binciken kwatancen ayyukan analgesic na dermorphin, [DPro6] -dermorphin, da C-terminal tripeptides [J]. Izv Akad Nauk Ser Biol, 2007 (5): 577-582. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18038625&query_hl=1
[4] Sandrini G, Degli UE, Salvadori S, et al. Dermorphin yana hana kashin baya nociceptive flexion reflex a cikin mutane [J]. Binciken Kwakwalwa, 1986,371 (2): 364-367.DOI: 10.1016 / 0006-8993 (86) 90376-8.
[5] Salvadori S, Marastoni M, Balboni G, et al. Haɗin gwiwa da aikin opioid na dermorphin tetrapeptides masu ɗauke da D-methionine S-oxide a matsayi na 2 [J]. Jaridar Magungunan Chemistry, 1986,29 (6): 889-894.DOI: 10.1021/jm00156a003.
Samfura akwai don amfani da bincike kawai:
